LOCUS       NZ_CP128617             4074 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Escherichia coli strain WL297 plasmid pWL297-4, complete sequence.
ACCESSION   NZ_CP128617
VERSION     NZ_CP128617.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35977202
            Assembly: GCF_030384885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Jiang,T.
  TITLE     Complete genome sequence of a carbapenem resistant E. coli isolate
            WL297
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Jiang,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) Department of Clinical Laboratory, The
            Affiliated Wenling Hospital, Chengxi, Wenling 317500, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/04/2023 08:21:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,887
            CDSs (total)                      :: 4,768
            Genes (coding)                    :: 4,605
            CDSs (with protein)               :: 4,605
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 50 of 163
            Pseudo Genes (incomplete)         :: 116 of 163
            Pseudo Genes (internal stop)      :: 28 of 163
            Pseudo Genes (multiple problems)  :: 29 of 163
            Pseudo Genes (short protein)      :: 3 of 163
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WL297"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pWL297-4"
                     /geo_loc_name="China: Wenling"
                     /collection_date="2021"
                     /collected_by="JT"
     gene            complement(join(3872..4074,1..499))
                     /locus_tag="QU520_RS24425"
                     /old_locus_tag="QU520_24425"
     CDS             complement(join(3872..4074,1..499))
                     /locus_tag="QU520_RS24425"
                     /old_locus_tag="QU520_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1032..1286)
                     /gene="mobC"
                     /locus_tag="QU520_RS24430"
                     /old_locus_tag="QU520_24430"
     CDS             complement(1032..1286)
                     /gene="mobC"
                     /locus_tag="QU520_RS24430"
                     /old_locus_tag="QU520_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1465..3630
                     /gene="mobQ"
                     /locus_tag="QU520_RS24435"
                     /old_locus_tag="QU520_24435"
     CDS             1465..3630
                     /gene="mobQ"
                     /locus_tag="QU520_RS24435"
                     /old_locus_tag="QU520_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_289787453.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEVLPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGLAALRAQL
                     LKREDELRRKELEDTERSRAERRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFESVDLIRDRSGYICDRSEIGQHPPLQRIYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAVVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRPDRGWSQGM"
                     /name="mobQ"
     Typing          complement(1288..1464)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1465..1823
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          complement(2..499)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.399"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1379..3621
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.326"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgatagctat cgttctgtca ccggtgctgc ggaactgctg gagcagctcg taaatgcgga
       61 tgctgtaggt gctggagagg ctggagacgt tcttcacgac tacccgcgta aactgcccct
      121 tgagctgtgt cagatacggc atgatggcat cggagaaggt gatctctact ttcgcctcac
      181 ctttgaaata ctgcgcccgg tactgcaccc agcggaactc ttctcgtttt tcctcgttct
      241 tgatgattac tgacctgtcc cagaggcgat ctatcgcttc cttgagccta gcttcagcgt
      301 gctgccgtcc catgtcagga aaggcatcat gaaactctgc agccgtgatg gtcatggttt
      361 tttgttcatc cggcgaaggg gaatcttctc cggatttcag gcggctgata cagaccagca
      421 ggaagcgctg ttcctgcaaa gtgagcttgt agcttgcctc gataagggcg tttgatttgt
      481 aagctatcag atctgacatt tcgcttgctc gttgctattg gtggcgtttt tttactgtac
      541 gccacctatc atattggtgg aagttttttt tttgcaaccc cttttttctg tggattacac
      601 ccccaaaagc tccacttatc acccccaaaa gctccaccta tcacccccaa aagctccacc
      661 tatcaccccc aaaagctcca cctatctcgc cagaacacct ttaaaatcat gtggttacaa
      721 gacctaaaaa ggttttaaaa aaaaagatct aaaaataaga tcttaaaaac ttaacggcga
      781 gcctgtggat aacttggggt gctgaaaata aaaatccaat aaaaacaatg tgttgcaaaa
      841 aaccaggatt gaagattccg aatacacccc ctccaaccct gtggaaatcc agtattcatg
      901 cgggttcagc gcatatcacc tgtcgaaaaa ccgtgcgtat gtgctcaaca tggcgactga
      961 ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg gtcgctgggc tgcggcgagc
     1021 ggacgataca atcactgctc tggcagcgcc tccagctcgt taatcagcgg cagtattcgc
     1081 gtcacatcag cctgccgtgt cacctctgcc ttcaggaact ggagtaacca ggctcgcaac
     1141 tgcacttcct ccggctttct gacctttgcc agcaatgctg cacccacaat gaccttctgc
     1201 gctgtgtcca ggctccggct cttcgccttc aggcgctgta atctcgcctc agcatcagca
     1261 atcttctgtt cgagcgttct gctcatgatg taactccatg cgcgatgaaa aatcgcattt
     1321 tagcgcgtca ctggtagttt aaaaactgag ctggcataat gcacggcaaa tcacgaagtg
     1381 cgcacttata caatctccac tccgtttcga ttgtgtgcgc cctgcggggc tgaaagaaaa
     1441 ctgcaaaaag gcattacggc agaaatggcg atttatcatc tcagtatgaa aatcatctcc
     1501 agaagcaacg gctacagcgc cgttgcttct gctgcctatc gttctggttc actaatgctt
     1561 gatgagcgta ccggactgac tcacgactac acccgcaaaa gcggtgtggc tgaggctgtg
     1621 attctgaccc ccgcgacagc ccctgcctgg tgtacgaacc gtgccgaact ctggaatgcc
     1681 gttgagaagg ccgaacgccg gaagaactca caacttgcgc gggaaattga actcgccatc
     1741 ccccacgaac tgccgcagga tgccgcacgg gaaaccgttc tcgcctttgt cagagaaaac
     1801 tttgtcagtc agggcatgat tgctgatgtg gcgttccatc acatggataa aacaaatccc
     1861 catgcgcaca ttatgctgac cacgagggct gtcggacctg cggggttcgg cggaaaggtc
     1921 agggactgga acgaccggac acatgccgag acgtggcgtg catcatgggc tgaccatgca
     1981 aacagggcac tggcgaacgc cggttatcag aaagagatag accatcgttc atacgagcga
     2041 caggggcttg agaaaacgcc gggtattcac ctcggaaagt ctgcctgtgc gatggaaaca
     2101 cgcggtatcg aaacagaacg cggcgaacag aatcgtctga ttaacaggct taacctggaa
     2161 atacagattt cccgcaccga actcaggaac agcgggctga cagagcctgc ccgccgtgtg
     2221 gctgaccttc tgaatatcgg gcttcctgac aacgcaactt cgtacacact acgtgacatc
     2281 atcgaagtgt tgccgaagga cagtaacgcg gcatggaaac tcaccagtaa cgccatgtcg
     2341 atgatggcag atatgcaggc gacgcgcaga cgctgggaag agctgaacac cgaacgaaag
     2401 gaagcgatga gccatgctgc cagcctgaaa aaatcatcgc cgttttcgtc cggtttttcc
     2461 cgtattcccc tgatgcaatg ggctgcaccg gaataccgta gtgagcaggg aaaaatccag
     2521 tctctgagcc agcaaatgga aaaattgcga cagcattacc ggaaggtgga gaaacaggac
     2581 attcccgcga gtcagacggc atttgaaaaa cagtggcagc agtgggggga atccggcctt
     2641 gcagctctca gagcccagtt gcttaaacgt gaagatgaat tacgccggaa ggagctggag
     2701 gatacagaac gtagtcgcgc ggagcgacga gcggaacagc tcaggaaaaa tgacaatgcc
     2761 gttatggatt caggggtgct gagagcagtc gttacggggt atggcgaggc tcccatgcct
     2821 ggtaacgggg aaatgacttg ttaccttctg cttcacaacc ggaacggaga atacacgctc
     2881 tgggggaacg agctggagaa ataccgcacc aggatctttg agagtgttga tttaatacgt
     2941 gacaggagtg gttatatctg tgaccgaagc gaaatcggtc aacatccacc actgcaacgt
     3001 atttattcct ctgcgacctt tgagcaactt ctgacgcagg tttgccagac atggccgcaa
     3061 catacccgca atctccgcca gccgaagaca tggccggagt ctttctgtct tggagaagac
     3121 cgccagccag ccatgccatc actggcagca aggaaggtgg attttacgca gggacgtctg
     3181 ccaccgacgc tgatgccggt catgagttct gtcgaccggg aaacccgcca gcttcagctt
     3241 ttgctggtca tgggcgtgga tgactctctg ggcggtgttg tccggcttaa tggtacgctg
     3301 tatcccgcgt ttgccgttcc ctcagccgat aacagccagc tggttatcag tgcactgacc
     3361 gataaggggt tacggtatgc agggtatggt gttgccgtaa atcatgatgc agacagccac
     3421 atatcacctg caccggaact tatggagttt catctgaaaa cccgtgaggc accgcttttt
     3481 gctgtcgtca acacaccaga aaaacagccg gatcatctgt tccgctcgct gggtttcaat
     3541 cgtacctggg acgaatggcg gcgcgaggaa gatgcacgaa cacacgccac cgagaggaga
     3601 cctgatcgcg gttggtcaca gggaatgtga acaatatttt gttttgttta acatcgtgtt
     3661 atgttcgtcg atgttcatcg acagggatga atgcaagaag cacactcaca tagcgcaaac
     3721 atagttttgt ttttccgccc ccaaaaattg ggggttcttt ttttgctgca tcagagcgtt
     3781 tttgacgcaa cttttcactt caatagtacc ttcgcattcc tgacacaaga aaaacgcgac
     3841 agtgagcgtt ttaggtgcct taaaatcgat tctagatcgt catcttgatc tgtttgtctt
     3901 ccttgaagcg aaagtgcagg gctacaaccg tgcgcccctt tttgatcgtc tcaaccgtta
     3961 caaccagatc gcttttttcg ttcagttcgg cgatggctgg cttgattatc atcttattca
     4021 agtctttgaa ttgttgatat ttatcttcaa cggcaagcat ggttcggaaa tcat
//
