LOCUS       NZ_CP128616             4445 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Escherichia coli strain WL297 plasmid pWL297-3, complete sequence.
ACCESSION   NZ_CP128616
VERSION     NZ_CP128616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35977202
            Assembly: GCF_030384885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Jiang,T.
  TITLE     Complete genome sequence of a carbapenem resistant E. coli isolate
            WL297
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Jiang,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) Department of Clinical Laboratory, The
            Affiliated Wenling Hospital, Chengxi, Wenling 317500, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/04/2023 08:21:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,887
            CDSs (total)                      :: 4,768
            Genes (coding)                    :: 4,605
            CDSs (with protein)               :: 4,605
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 50 of 163
            Pseudo Genes (incomplete)         :: 116 of 163
            Pseudo Genes (internal stop)      :: 28 of 163
            Pseudo Genes (multiple problems)  :: 29 of 163
            Pseudo Genes (short protein)      :: 3 of 163
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128616.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WL297"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pWL297-3"
                     /geo_loc_name="China: Wenling"
                     /collection_date="2021"
                     /collected_by="JT"
     gene            1412..1615
                     /locus_tag="QU520_RS24400"
                     /old_locus_tag="QU520_24400"
     CDS             1412..1615
                     /locus_tag="QU520_RS24400"
                     /old_locus_tag="QU520_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041634.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EELQNSIQTHFEAQYGIGVEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2584..2940)
                     /locus_tag="QU520_RS24405"
                     /old_locus_tag="QU520_24405"
     CDS             complement(2584..2940)
                     /locus_tag="QU520_RS24405"
                     /old_locus_tag="QU520_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_024168129.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFNTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDNSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(3143..3460)
                     /locus_tag="QU520_RS24410"
                     /old_locus_tag="QU520_24410"
     CDS             complement(3143..3460)
                     /locus_tag="QU520_RS24410"
                     /old_locus_tag="QU520_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001183575.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGIEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3647..3889
                     /locus_tag="QU520_RS24415"
                     /old_locus_tag="QU520_24415"
     CDS             3647..3889
                     /locus_tag="QU520_RS24415"
                     /old_locus_tag="QU520_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229898.1"
                     /translation="MALVRSGNKSKIFEADKKFNSVFDTEYQPGETCKYEGIYKCTGCG
                     KEVTIAGGKKIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            4009..4335
                     /locus_tag="QU520_RS24420"
                     /old_locus_tag="QU520_24420"
     CDS             4009..4335
                     /locus_tag="QU520_RS24420"
                     /old_locus_tag="QU520_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001388929.1"
                     /translation="MQESQENNSKPPAWRLFYLYLQLTLIYHSIHCIYSVLIGVFMSNE
                     LDSLSEVVKGLTKIELPGGGGGETDSPVVEAAIAVGIVGAAAYGIYKLFGRAEPQKDED
                     KLGF"
                     /name="hypothetical protein"
     Typing          complement(1212..1290)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.5"
                     /coverage="83.0"
                     /evalue="7.81e-24"
     Typing          1864..2255
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.924"
                     /coverage="91.0"
                     /evalue="3.73e-173"
ORIGIN
        1 aacagtgagc catgtagatg gttggaatgg ccttcctcaa tacgaaatca ggggaaataa
       61 aatctacagg acagtgagcc atgtagatgg ttggaatggt cttcctcaat acgaaatcag
      121 gggaaataaa atctacagga cagtgagcca tgtagatggt tggaatggtc ttcctcaata
      181 cgaaatcagg ggaaataaaa tctacacaac agtgagtcat gtaaacggtt ggaacggtct
      241 tccgcaatat gagatcagat aatttgcggg gcaaaccctg caaaaagaaa tcatatttac
      301 cataacatct gtattgtacg caccgttaaa acgcgctcag cgcgattctg gcgcaaaaac
      361 cgtaaaaagg gatgttttcc ccggggtaaa ccggaaaaat gcgtcaggat tgttttcagc
      421 gcgttgcatg actatgcatg aaactgaatg gcaatcgatc cgggcgcgtc cgctgtccct
      481 gaggcttatt tttggcagtt ttctttctgt ctgcgtcaca ttccatgcca gaacacatcc
      541 gtcagcaacg acattcaaca gcccaccatt agcccacaca caaaataccg atgggcacac
      601 cgatgtatca taaaaattta tccgttatat ttcatacaaa taagaggcca ccatcagccc
      661 actctattag cccacaaaac gcccacactt ttgctatcac tatgattatc aatataaaac
      721 ccacaaaagt gggctgatga tgggctaact gtgggcgtag aagcccatca tcagcccacc
      781 acatccgaca aaatagaatt ttttgccaga taaacaccac tataacgcac tgaatttaaa
      841 gtaaaatcac gcaaaacgca gttttgcgac aggtgtggta aatgcaggtc agaggagagg
      901 ggaatttcag gcgcggagcc ggtcctggtt cgggctacag gactctcaga aaaagcgaat
      961 aaatttgcgc tggccgtcag agcattacgc cggggtatcg gggtgaaacc ctgagcagga
     1021 ggtaattgta atagcgtcac gggtgacggt attacaatta cacatcctgt cctgtgtcag
     1081 gcagccggat acggtcgtcc tggtggcaac agtcgggcag ggggtacaac tgtaccccgt
     1141 cacatccgca aaagatttac ggcacgaaac aggagcgacg caacaactgc cggtaacacg
     1201 accccatgaa cgggacagga tgtgtaatcg tataaccgcg cacgcacgtt catccgatta
     1261 caacctggtc agggctttac cccgacaccc acgatgtgat gaacggatga acataaaaca
     1321 accggaaaaa cgctcactgt ccaggcaaag aacggaacac ggcataatca catgatgctg
     1381 aatacagaga cagaacggag aacgacagaa ggtgaacaaa cagcagcaaa ctgcacttaa
     1441 catggcgcga ttcatcaaaa gccagagtct gacactgctt gaaaagctgg atgccctcga
     1501 tgccgacgaa caggcaacca tgtgtgaacg gctgcacgaa ctggcggaag aactgcagaa
     1561 cagcatacag acgcattttg aagcgcagta cggcattgga gtggaacaac cataaagacg
     1621 ctggcgaaag cggtcgcggc ttgtccgtga gcgttttcgc cagcagacaa gcccgcaggg
     1681 cgcgtcagga cagtgacctg agaaagacac tggcgcgtaa gtgctcaaag caaacgggcg
     1741 gagaaattcc gcttcctcgc ccactcgctc actacgttcg gtcgctgggg ctgcggcgag
     1801 cggtggcggc tctctcataa ctgcagacgg gttatccaca aaattggggg acaaccagga
     1861 cagaacaaag gccgcggcag agccgttttt ccataggctc cgcccccctg acgagcatca
     1921 caaaaacaga cgctcaagtc agggggaggc gaaacccgac aggactataa agataccagg
     1981 tgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactggaagg acagtatttg
     2341 gtatctgcgc tctgctgaag ccagttacca ggttaagcag ttccccaact gacttaacct
     2401 tcgaaaaacc gcctccccag gcggtttttt cgtttacaga gcaagagatt acgcgtacag
     2461 agcacggatc tcaagaagat cgtaagaaga aagttaacat gagtgttttt atccctcaaa
     2521 aagaaggctt ctataaatag aagccttctt tttgagggat aacttatatt tttttaaaaa
     2581 aaatcatgca atagcatgtt tcttctcaga attatcaaca atgtgtttcc tttctatgat
     2641 ttcatgagcg ggaatcactc tgtctatttc ttcttgcaat cgagggctac tagtaacctc
     2701 ccataaatca acagactgct gtatatttag ccagaacttt gcagtggtat tgaaggcttt
     2761 agccaaccta aaagccatat cggtagacag ctttctatta ttgttaacta aagcactggc
     2821 agagtttcta tgcacattca acatttcagc caagtcattg atttttaaac cacttggttc
     2881 gagatattca taaagaataa tatcccctac agttgatggc tttctagttg cttgggacat
     2941 gctttactcc taaatttttt acttctctct gttagatgag gaagataagt atttcgtcta
     3001 ttaccagctg tagcgatgag taattatctt aaatatagtc acgtcattta gtttattaat
     3061 cattatttgt aatgacattt taatgtaggt gagggaacgc ctcacctact tgttttataa
     3121 gataacagcc acgacctgtt atctatcggt aatcatgtgg atcgagatac aaatcctcga
     3181 ttccgttttc accccactta aatattaagc ggtacttctc attcacccgt atggatgaat
     3241 accctgtcaa tggagggttg agattctcaa atcggtttcc cgggggagac cgcaaatctc
     3301 caactttatc agcagcattc agcatatcca gcttgcgacc taatgaagca ctaatcgcag
     3361 ttggaatctt tcgattggct ttaccatatt tgtaaaactg gtatagccaa tcgtctcgaa
     3421 actgaatgcg gcaatcatca tgttcatgat cttgctgcat ttttctttta cctcaatttt
     3481 tgccgcaact tggcaatttt ttagatcacc ttcttacctc cgatatagta cagcaaacac
     3541 aagaaatgca caacagcaca gtgcacttgt gcaaccaata accatactgt agaatgcacc
     3601 cacacacagt gcagatgtgc aaaatagagg gtaatttgga ggttacatgg ctcttgttcg
     3661 ttcaggaaac aaaagcaaga tttttgaagc agataagaag tttaactcgg tttttgacac
     3721 tgaatatcag ccgggagaaa catgtaaata tgaagggatt tataaatgta ccggttgcgg
     3781 taaagaagta actatcgcag gtgggaaaaa aatcccacca caaaaccacc accaacatac
     3841 ccccgcgcaa gggaacattc gttggcaatt gtttgtttat gctcagtgag gaatagaaaa
     3901 tggcagatcg tcgaccgctc aacccacacg ttgtcgttcc taaaccaggc ggcggaactc
     3961 aacctcgtga ccgtaacaat gacggaaggg tcagaggaaa acgctcagat gcaggaaagc
     4021 caagaaaata acagcaagcc gccagcctgg cggctttttt atttatatct acagctgaca
     4081 ctgatttacc atagcatcca ctgtatctac tcagtcttga taggtgtttt tatgagtaat
     4141 gaattagaca gcctttcaga agtagttaaa ggactcacaa agatagagct gcctggcggc
     4201 ggtggcggtg aaaccgatag ccctgtagtt gaggcagcta tagctgttgg cattgtaggg
     4261 gctgctgcat atgggattta taagcttttt ggtcgcgcag aaccccagaa agatgaggac
     4321 aaactcggtt tctagaacca taaggcagca tcaaaacggc attcatttac cataacatcc
     4381 gttaactgcg gccttgtcat aaaagaaatg gatattggag acaatatgag caataagatc
     4441 tatag
//
