LOCUS       NZ_CP128612            10682 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Escherichia coli strain HZ13 plasmid pHZ13-3, complete sequence.
ACCESSION   NZ_CP128612
VERSION     NZ_CP128612.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35889837
            Assembly: GCF_030384845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Jiang,T.
  TITLE     Complete genome sequence of a carbapenem resistant E. coli isolate
            HZ13
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Jiang,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2023) Department of Clinical Laboratory, The
            Affiliated Wenling Hospital, Chengxi, Wenling 317500, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/04/2023 08:34:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,071
            CDSs (total)                      :: 4,949
            Genes (coding)                    :: 4,707
            CDSs (with protein)               :: 4,707
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 67 of 242
            Pseudo Genes (incomplete)         :: 171 of 242
            Pseudo Genes (internal stop)      :: 40 of 242
            Pseudo Genes (multiple problems)  :: 36 of 242
            Pseudo Genes (short protein)      :: 5 of 242
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128612.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="HZ13"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pHZ13-3"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2021"
                     /collected_by="JT"
     gene            join(10054..10682,1..352)
                     /locus_tag="QU238_RS25295"
                     /old_locus_tag="QU238_25295"
     CDS             join(10054..10682,1..352)
                     /locus_tag="QU238_RS25295"
                     /old_locus_tag="QU238_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_000019402.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     gene            complement(461..1405)
                     /locus_tag="QU238_RS25300"
                     /old_locus_tag="QU238_25300"
     CDS             complement(461..1405)
                     /locus_tag="QU238_RS25300"
                     /old_locus_tag="QU238_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_100183899.1"
                     /translation="MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQARYASD
                     NTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHTTIYS
                     TIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHW
                     EADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKALTEALKYLPAELRKTLTY
                     DRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYL
                     NQVAMSLNTRPRKALDWLTPLGKVRISGEILLG"
                     /name="IS30 family transposase"
     gene            complement(1682..4699)
                     /locus_tag="QU238_RS25305"
                     /old_locus_tag="QU238_25305"
     CDS             complement(1682..4699)
                     /locus_tag="QU238_RS25305"
                     /old_locus_tag="QU238_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /protein_id="WP_004199413.1"
                     /translation="MAFEERVQILSEAEQDELYGPPAFTSADQRFFFSLNDKELAIAKS
                     LRHRGQRYMLVVLLGYFKAKPVVLNPGFHQIKQDLKYVYQTVLPGPGCRPFNLTPKENE
                     RIYQRVFQLCNYQRWNVKDHGAALRDYLSQQARAWTAPRHLFDAANEYCSGQKIAIPAY
                     STLQKIISQVVGDEQEHMAAHLERAMSRGLKQALAELVNGTGPLPFRQLRQSARNFTGT
                     ELEKELIVYRHIQHWMPEVDLLLSTLSLSQKNLQHLAEKVDYYGAKLKRQTVGSQWLYL
                     LCYLQTRWQQALERIADGFVHHVRQTKQKAKDYAQEAVFKDWQKAAKNVSKAAEVLHLF
                     IDDSIDLQLPFATVRQQALSLLTKRDLESVCLFLNEQRRSVDEAMWQYCDEKESLRKGL
                     LRELFLCLRFEGCDGTQHLAAALAKTQNELNGQDAQLQTADTRLLSKKSREFLLDGEGN
                     ILIDRYEWFLYQQIPDRLNGQLTLPDITKYRALDADLIDGEHWRKNKYTLLQQSHFTKL
                     AEEPEKLIKQMAMELDTRLYEVGEYLEQEDNRNIILRNPQGKRFWRLPSASKHHLVNNP
                     FFQQIPTTGIADVLRMVDRDTGFIDCFAHVLGSQSRSRSHEYDLLAILVGNATNQGIYG
                     MAQISDRTYDQLSTIQANYLRLETLNAANDNINNATAKLPIFRYYNIQEDVIHASADGQ
                     KFEARRETFKTRYSSKYFGTQKGVSAMTLIANHAAINARVIGANEHESHYIFDLLMSNT
                     SDIIPDVLSTDTHGVNHVNFALLDLFGYQFAPRYAQVGKVINDMFDVKEDKEHRIQLCL
                     KKPINTHRIAQHWDTIQRIAVSLKQRKTTQATLVRKLSEYKRNHPLLEALTEYNRLVKA
                     NYLLCYIDDASLRNYVQRALNRGEAYHQLRRAVSSVNGDQFRGSSDEEIQLWNECARLV
                     TNAIVYFNSRILSQLLTSFEYQGDTKRIDIVKQASPVAWHNINLKGTYHFELSEKLPDL
                     EELMRSIEGYLPVSEK"
                     /name="Tn3-like element IS3000 family transposase"
     gene            complement(<4841..5317)
                     /locus_tag="QU238_RS25310"
                     /old_locus_tag="QU238_25310"
                     /pseudo=""
     CDS             complement(<4841..5317)
                     /locus_tag="QU238_RS25310"
                     /old_locus_tag="QU238_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            5419..5658
                     /locus_tag="QU238_RS25315"
                     /old_locus_tag="QU238_25315"
     CDS             5419..5658
                     /locus_tag="QU238_RS25315"
                     /old_locus_tag="QU238_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001549892.1"
                     /translation="MPRHYEIDSAWRASIKREPNGRQTVTTEAFVSQLALINFHWSCRQ
                     ANQWIETYVTVFKDISTQEGENRTFMLFNPNGGR"
                     /name="hypothetical protein"
     gene            5661..6017
                     /locus_tag="QU238_RS25320"
                     /old_locus_tag="QU238_25320"
     CDS             5661..6017
                     /locus_tag="QU238_RS25320"
                     /old_locus_tag="QU238_25320"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="WP_004199403.1"
                     /translation="MGFPSPATDYQENRLSLDQHCNTGAPGVFFFRSDTYSFREGIKPG
                     ALLIVDFGGTPVDGSLVLCVLEQEFRIMRLRLHPKRCLQELDKLDNFRAIPDDDEDGLE
                     VRGVITHIVALLQS"
                     /name="S24 family peptidase"
     gene            complement(6051..6755)
                     /locus_tag="QU238_RS25325"
                     /old_locus_tag="QU238_25325"
     CDS             complement(6051..6755)
                     /locus_tag="QU238_RS25325"
                     /old_locus_tag="QU238_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            6814..6948
                     /gene="cutA"
                     /locus_tag="QU238_RS25330"
                     /old_locus_tag="QU238_25330"
     CDS             6814..6948
                     /gene="cutA"
                     /locus_tag="QU238_RS25330"
                     /old_locus_tag="QU238_25330"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_023408307.1"
                     /translation="MPPALQERLRQLHPYELPELLAVEAASGLPEYLQWLAAESRPVN"
                     /name="cutA"
     gene            6953..>7327
                     /locus_tag="QU238_RS25335"
                     /old_locus_tag="QU238_25335"
                     /pseudo=""
     CDS             6953..>7327
                     /locus_tag="QU238_RS25335"
                     /old_locus_tag="QU238_25335"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /name="protein-disulfide reductase DsbD family protein"
     gene            <7427..7984
                     /locus_tag="QU238_RS25340"
                     /old_locus_tag="QU238_25340"
                     /pseudo=""
     CDS             <7427..7984
                     /locus_tag="QU238_RS25340"
                     /old_locus_tag="QU238_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation (TAT) pathway signal
                     sequence domain protein"
                     /name="twin-arginine translocation (TAT) pathway signal
                     sequence domain protein"
     gene            complement(7995..8633)
                     /locus_tag="QU238_RS25345"
                     /old_locus_tag="QU238_25345"
     CDS             complement(7995..8633)
                     /locus_tag="QU238_RS25345"
                     /old_locus_tag="QU238_25345"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /GO_function="GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="WP_004201168.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="phosphoribosylanthranilate isomerase"
     gene            complement(8638..9003)
                     /gene="ble"
                     /locus_tag="QU238_RS25350"
                     /old_locus_tag="QU238_25350"
     ARG             complement(8642..9003)
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9007..9819)
                     /locus_tag="QU238_RS25355"
                     /old_locus_tag="QU238_25355"
     CDS             complement(9007..9819)
                     /locus_tag="QU238_RS25355"
                     /old_locus_tag="QU238_25355"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase NDM-5"
                     /protein_id="WP_023408309.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="subclass B1 metallo-beta-lactamase NDM-5"
     ARG             complement(9011..9819)
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(8639..9003)
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gcatggagag gagcaatttg tctcagccga tgccggctac caaggggcgc cacagcgcga
       61 ggagctggcc gaggtggatg tggactggct gatcgccgag cgccccggca aggtaagaac
      121 cttgaaacag catccacgca agaacaaaac ggccatcaac atcgaataca tgaaagccag
      181 catccgggcc aaggtggagc acccatttcg catcatcaag cgacagttcg gcttcgtgaa
      241 agccagatac aaggggttgc tgaaaaacga taaccaactg gcgatgttat tcacgctggc
      301 caacctgttt cgggcggacc aaatgatacg tcagtgggag agatctcact aaaaactggg
      361 gataacgcct taaatggcga agaaacggtc taaataggct gattcaaggc atttacggga
      421 gaaaaaatcg gctcaaacat gaagaaatga aatgactgag tcagccgaga agaatttccc
      481 cgcttattcg caccttccct aatggtgtaa gccaatctaa cgcctttcta ggacgagtat
      541 tcagtgacat ggcaacttga tttaaataat gctgatctgc ctgatttaaa tcaatccctt
      601 taggtaaata ttgcctaatt aaaccattca tattttcgca tgtgcctttt tgccagggtg
      661 aatgtgggtc acagaaatat acatctatgc ctaaatcttc ttcgagtatt ttatgttctg
      721 acatctcacg tccacggtca taggtcaacg ttttacgcag ttctgcaggt aaatatttca
      781 gagcttcagt taaagccttg cgcactgatt ctgcctttgc atcaggtaat gttgccaaga
      841 tacagagccg tgtatttcgt tcaataagtg ttgctatcga acttttattg tctttacctt
      901 taattaaatc agcttcccaa tgacccggta tttttctttc ttgaacttcg gctgggcgct
      961 catgaatagt tttaatatcc tgtaatatag aatctttttt aggttcaccg ttagcttttc
     1021 gctttttatt ttcatgacgt agacaggata ataagtcttt tttcaactca ccctttggta
     1081 atgctcgtat cgttgaataa atcgttgtat ggcttacatt cattgtttga tccaaatcag
     1141 gaaatgtctt taaacgcttt gctatttgct gaggagacca taaacaacgg atcgcttcaa
     1201 caataaattt ccagaggatt gaatcgattt tgagttttct gtgaccacgt ctacgtctag
     1261 cgaaggtgtt atcagaagca tatcgagctt gataaacgtc attgatgcta tttcttttaa
     1321 gctcacgata gatcgtacta ggatgtcttt taatgagttc agcaaatttt ctggctgaaa
     1381 agccttcttt tcttgactca agcattaatg cagtacgatc ttcaaagtta agatgatggt
     1441 atgacaattt tatatactcc ataaaccctt taaattaatt aggtggttta tgtcgcactt
     1501 caagttttac tctgccaact ttaaatcagt ggctccacta acactcttca cttatttatt
     1561 taaaatttag cgacgttaaa agacaattaa gtatttttag ataatggggt tctagggatt
     1621 ttccgtccaa aaacgacaaa gtgctctgga ggccgcgccg tccgtggcct ccagaggggt
     1681 attacttttc gctgacgggt aaatatccct cgattgagcg cataagctcc tccagatctg
     1741 gcaatttttc gctcaattcg aagtggtaag tccccttgag gttaatgttg tgccaggcca
     1801 caggggatgc ctgtttgacg atatctattc tcttggtatc tccttggtat tcgaagctgg
     1861 tcaacagctg gctgagtatc ctggagttga agtaaacgat ggcattggtg accaggcgag
     1921 cgcactcatt ccatagctgg atttcttcgt ctgaactgcc ccggaactga tccccattga
     1981 cgctgctcac ggcccgacgc agttggtgat aggcctctcc ccggttcagc gcgcgctgaa
     2041 catagtttct taaactggca tcatcgatgt agcacagtag ataattcgct ttcaccaggc
     2101 gattgtattc cgtcagggct tccagcagcg ggtgattgcg cttgtactcc gagagctttc
     2161 tcaccaaggt ggcttgcgtt gttttccgct gcttaagtga tactgcaatc cgttggatgg
     2221 tatcccagtg ctgcgcaata cgatgggtat tgattggctt ttttaagcac agctgaattc
     2281 ggtgttcttt gtcttccttg acatcaaaca tgtcattgat cactttgcca acctgggcat
     2341 agcgtggggc aaactggtat ccgaacagat ccagtaacgc gaagttcaca tggttcaccc
     2401 catgggtatc ggttgagagc acatccggaa tgatgtctga cgtattgctc atcaacaaat
     2461 caaagatgta gtgcgattca tgttcgttgg cgccgatcac tctggcgttg atcgcagcgt
     2521 gattggcgat caaggtcatg gcagaaacac ctttttgagt gccaaagtac ttcgacgaat
     2581 aacgggtttt gaaggtctcg cgccgggctt cgaacttttg accatcggca ctggcgtgga
     2641 tcacatcttc ctggatgttg tagtaccgga agatgggtag cttggctgtc gcgttattga
     2701 tgttgtcgtt agcagcattc aatgtttcca ggcgaagata gttcgcctgg atagtgctga
     2761 gctgatcata ggtacgatca gagatctgtg ccatgccgta aatgccttga ttggttgcat
     2821 tgccgaccag aattgccaac aggtcatatt catgggaacg gcttctggat tgggaaccca
     2881 gcacatgagc gaagcagtca atgaaaccgg tgtcacgatc aaccatgcgc agtacatccg
     2941 caatccccgt tgtgggaatt tgctggaaga agggattgtt gaccagatga tgtttgctgg
     3001 ccgaaggcag gcgccagaag cgtttaccct gcggattacg caagatgata ttccggttgt
     3061 cttcctgttc aagatattcg ccgacttcgt acaaacgggt atccaattcc atggccatct
     3121 gtttgatcag cttttcaggc tcctccgcta attttgtaaa atggctctgt tgaagcagcg
     3181 tatatttgtt ttttcgccaa tgttccccgt cgatcaggtc ggcgtcgagt gcccggtatt
     3241 tagtgatatc aggcagcgtc agctggccat tcaggcgatc aggaatctgt tgatagagga
     3301 accactcata acgatcgatc aggatattcc cttcaccatc cagcaggaat tcacgtgatt
     3361 ttttggaaag gagtctggtg tcggcagttt gcaactgagc gtcctggccg ttgagttcgt
     3421 tttgtgtttt cgccaaggcg gccgctaagt gctgggtgcc gtcgcagcct tcgaagcgca
     3481 gacataggaa caactctcgc agcaaacctt tccgcagact ttctttctcg tcgcagtact
     3541 gccacatggc ttcatcgacc gatcgtcgct gctcgttcag gaagagacat acagattcca
     3601 gatccctttt ggtcagcagg ctcagtgctt gctgtcttac tgttgcaaac ggtagttgca
     3661 gatcaatgct gtcatcaatg aacaggtgca gtacttcagc tgccttgctg acatttttag
     3721 ctgctttttg ccaatcctta aacaccgctt cctgcgcata atcctttgcc ttctgtttgg
     3781 tctgtctgac atgatgcaca aagccatcgg caatacgttc cagcgcttgc tgccatcgcg
     3841 tttgaagata gcacaataaa tacagccatt ggctgcctac ggtttgacgt ttcagtttgg
     3901 caccgtagta gtcgactttc tctgccaggt gttgcagatt tttctgcgac agtgatagcg
     3961 tgctcaacag cagatccact tccggcatcc aatgctggat atggcgatag acaatcagtt
     4021 ctttctccaa ctcggttccg gtgaagttac gagccgattg tcgcaactgt cggaacggca
     4081 gtggacctgt gccgttcacg agctccgcca gtgcttgctt aagaccgcgt gacattgcac
     4141 gctcaaggtg cgcggccatg tgttcctgtt cgtctcccac cacctgactg atgatctttt
     4201 gcagcgtgct gtaggcaggg attgcgatct tctgccccga acaatattca ttggccgcat
     4261 cgaagaggtg cctaggcgct gtccaggctc tggcttgctg ggataagtaa tctctcaacg
     4321 ccgctccatg gtctttgaca ttccagcgct ggtagttgca gagctggaaa acacgttggt
     4381 aaatccgttc gttctctttt ggcgtgagat taaagggtct acagcctggg cctggtagaa
     4441 cggtttgata aacgtatttg aggtcctgct tgatctgatg aaagccggga ttcagcacca
     4501 ccggcttggc tttgaaatag cccagcagta ccaccagcat gtaacgctgg ccccgatgac
     4561 ggaggctttt agcgatcgcc agttccttat cgttcagcga gaaaaagaag cgttggtcgg
     4621 cggaggtgaa agcggggggt ccatataact catcttgttc tgcctcggac aagatttgga
     4681 cacgttcttc gaatgccatg gatttagccc aaaaagacta aatcttactc aaacagagcc
     4741 caaataggga tctcgaaaaa agtaataccc ctctggaggc cacagacggc ggggcctcca
     4801 gagcactttg tcgtttttgg acggaaaatc cctagaaccc cacattcata tgacgggcgg
     4861 ataatggctt ttttatccgg gcaagacctc gcttaactgc ccgggtcata atgccgtcaa
     4921 ccgcattcca actgagctta agttgcttcc tgacagcatc aacggtgctg attttcagcc
     4981 atgagagaac gaacgattcg aatagcaacg tataccggct tccggggcca gcccacggaa
     5041 caggcaacgt caggcagcca tgctccggac acataattcg tggaacatcg gcttcaacaa
     5101 tagtggtgaa ctggcaggta tcaagatggc gccatttacg atgacggtga tcgtgaacag
     5161 aacaggattt accgcaggtc ggacaggcta gccgggtgtt ttcagcgatc tcaatagtga
     5221 cagtaacaga accggcattt tcatcgagag aaagggactt tacctgccac ggatcggaca
     5281 ggttgagaat atgagcgtag agggactttt cgtccatggc ggtgacctct ggcgattaaa
     5341 tacaccatta tcatgccttc agccaccaca acaagggaag acccatatgc atacagctat
     5401 attgatcgga ggtttttcat gcctagacac tatgaaattg actcagcctg gcgtgcgagc
     5461 attaagcgtg agccgaacgg tcgtcagaca gttacgacag aagcctttgt cagccagctt
     5521 gcgctgatta attttcactg gagttgtcgc caagcaaatc agtggattga aacctacgtg
     5581 accgttttta aagacatttc cactcaggaa ggagagaacc ggacattcat gctttttaac
     5641 cccaacggag gccgttaaaa atgggttttc catcgccagc aaccgactac caggaaaaca
     5701 ggctgtcact cgatcagcac tgtaatacag gagcgccggg agtattcttt tttcgaagtg
     5761 atacctactc tttccgggaa gggataaagc ctggtgcgct gctcatagta gattttgggg
     5821 gcacgcccgt tgacgggtct ttggtgctct gtgtcctgga acaagaattc aggatcatgc
     5881 gattgcgact acaccccaag cggtgtctcc aggaactcga taagctggat aacttcaggg
     5941 ctatacctga tgacgatgag gacgggttgg aggtcagggg ggtcattacg cacattgtgg
     6001 cactgttgca aagttagcga tgaggcagcc ttttgtctta ttcaaaggcc ttacatttca
     6061 aaaactctgc ttaccaggcg catttcgccc aggggatcac cataataaaa tgctgaggcc
     6121 tggcctttgc gtagtgcacg catcacctca atacctttga tggtggcgta agccgtcttc
     6181 atggatttaa atcccagcgt ggcgccgatt atccgtttca gtttgccatg atcgcattca
     6241 atcacgttgt tccggtactt aatctgtcgg tgttcaacgt cagacgggca ccggccttcg
     6301 cgtttgagca gagcaagcgc gcgaccatag gcgggcgctt tatccgtgtt gatgaatcgc
     6361 gggatctgcc acttcttcac gttgttgagg attttaccca gaaaccggta tgcagctttg
     6421 ctgttacgac gggaggagag ataaaaatcg acagtgcggc cccggctgtc gacggcccgg
     6481 tacagatacg cccagcggcc attgaccttc acgtaggttt catccatgtg ccacgggcaa
     6541 agatcggaag ggttacgcca gtaccagcgc agccgttttt ccatttcagg cgcataacgc
     6601 tgaacccagc ggtaaatcgt ggagtgatcg acattcactc cgcgttcagc cagcatctcc
     6661 tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg
     6721 tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga
     6781 tgataagttt atcaccaccg actatttgca acagtgcccc ccgccctgca ggaacgcctg
     6841 cgccaactgc atccctacga actcccggag ctgctcgcgg tcgaagccgc gtccggcctg
     6901 cccgaatacc tgcaatggct ggccgccgag agccgaccgg taaactgagc caatgaccgc
     6961 atccacgatc cgcctgcgcc gctggctggc cgggctcgcc ctgctgcttg cgctgcccgc
     7021 gacctcggcc gtcgcccagg acttcgaact gccgccggtc gacgaggtct tcgtcctgtc
     7081 cgcgcaggcc accgcgccgg accgcatcga ggtgcgctgg cggatcgccg acggctatta
     7141 cctgtaccgg caccgcacct cggtcaaggc cgatgccgcc ttcaccggcg cgaccatggc
     7201 gctgccgaag ggcaaggcct accgcgacga attcttcggc gacgtcgaaa cctaccgcaa
     7261 ggaattgctc ggcaccctca ccggcacgcc cgcggccggc gcgagcgcga ccaccctgac
     7321 cgtgaagtac cagggctgcg ccgatgccgg cgtgtgctac ccgccgcaga cccgcaccct
     7381 gaaggtcgcg ttgccgggcg aagcgggcgc tggcggcttc ggctgtaagg cgcgcgggct
     7441 gcatgattat gtcgtcaaga acggctctgc cgatcatccc aacgccgagg tgaaattcgc
     7501 actgggtgat gtggtcaaca ccatgatcgg ctgcactaat ggtgaaacga tcatgctgtg
     7561 ccacgacacc tcgctgccgc gcccctattc tctcggcttt cgggtgcaag gcaccgaggg
     7621 gctgtggatg gacgtcaaca agtcgatcta tctggagggc aagagcccac agccgcaccg
     7681 ctgggagcct gccgagggct ggtttgcgaa atacgatcac ccgctatgga aacgctacgc
     7741 cgatctggcg gcaggggccg ggcatggcgg gatggactgg ttcgtgatcc acgcttttgt
     7801 cgaggcgctg aaggccaagg ccccgatgcc aatcgacatt tacgacgcgc tggcctggag
     7861 cgcgatcacg cctctgtcgg aacaatcgat tgctgagggc aatcgcacgt tagattttcc
     7921 cgacttcacc cgagggcagt ggcgcacccg caagccgatc tttgcgctga acgacgccta
     7981 ttgatcgacg cgatttaggc caagcgcacc gcaaaggcga aattggtaat cttgtcggta
     8041 tccttgacgc ccggcgcgct ttcgacgccg ctggaggtat cgaccagcgg cgctccggtg
     8101 cgcgcaatcg cctcggcaac attcgtcgga tttagcccgc ctgccagccc ccacggcaag
     8161 gcaccgcgat atccggccag cagcgaccag tcgaacgcca accccatgcc gccgggcagc
     8221 gcgcctttgg gggtcttggc gtcgaacaag atcaagtccg ccgccccggc ataggctgcg
     8281 gcgcgtgcga catcgctggc gctggcgacg ggcagcgcct tccacaccgg cttgccaaac
     8341 cgcgcgcgca actgggccac gcgttcgggc gattccgaac cgtgcagctg cagcgcgttc
     8401 agcttggctg ccaccagtgc gtcggcgatg acagcatcat ccgcatcgac gaacaaaccg
     8461 accatggcga tctggccagc tgcgcgcgat gtcaaagcgc ccgcgacatt cgacgtaacc
     8521 gcacggggcg acgctggata gaacaccaac ccggcatagt ccgcccgcgc cgcgatggtc
     8581 gcatcgagcg cctcgggtgt gctgatcccg caaatcttga ttttcgcggg catgcggtca
     8641 gtcggggttc tggatcagcc gcaccagcgt gcagtcggga tcgatcaggt agccgatcct
     8701 caggccgctc gcctccagtt gcggagcttt gaagcgcggc cagccggtgc ttttttcctc
     8761 ggctcccgcc gcgttcacca atgccaccat ggcatcgaga tcatccaacc gcaggcaaca
     8821 gccgaacgag ctcgtagctg ggtcgaggtc aggatagggg aagaattcga gctgcaaacc
     8881 gccgcgctgc aggatcatcc agccgcgatc cttccaactc gtcgcaaagc ccagcttcgc
     8941 ataaaacgcc tctgtcacat cgaaatcgcg cgatggcaga ttgggggtga cgtggtcagc
     9001 catggctcag cgcagcttgt cggccatgcg ggccgtatga gtgattgcgg cgcggctatc
     9061 gggggcggaa tggctcatca cgatcatgct ggccttgggg aacgccgcac caaacgcgcg
     9121 cgctgacgcg gcgtagtgct cagtgtcggc atcaccgaga ttgccgagcg acttggcctt
     9181 gctgtccttg atcaggcagc caccaaaagc gatgtcggtg ccgtcgatcc caacggtgat
     9241 attgtcactg gtgtggccgg ggccggggta aaataccttg agcgggccaa agttgggcgc
     9301 ggttgctggt tcgacccagc cattggcggc gaaagtcagg ctgtgttgcg ccgcaaccag
     9361 cccctcttgc ggggcaagct ggttcgacaa cgcattggca taagtcgcaa tccccgccgc
     9421 atgcagcgcg tccataccgc ccatcttgtc ctgatgcgcg tgagtcacca ccgccagcgc
     9481 gaccggcagg ttgatctcct gcttgatcca gttgaggatc tgggcggtct ggtcatcggt
     9541 ccaggcggta tcgaccaaca gcacgcggcc gccatccctg acgatcaaac cgttggaagc
     9601 gactgccccg aaacccggca tgtcgagata ggaagtgtgc tgccagacat tcggtgcgag
     9661 ctggcggaaa accagatcgc caaaccgttg gtcgccagtt tccatttgct ggccaatcgt
     9721 cgggcggatt tcaccgggca tgcacccgct cagcatcaat gcagcggcta atgcggtgct
     9781 cagcttcgcg accgggtgca taatattggg caattccatc aagttttcct tttattcagc
     9841 attaaaaacc ccgcaaatgc gaggcctagt aaatagatga tcttaatttg gttcactgta
     9901 gcaaaaatat ggggcgaatt caaacatgag gtgcgacagt ttcaaaagcc atatgataat
     9961 caacaagctg agcaaatttc tctaaggaag gtgcgaacaa gtccctgata tgagatcatg
    10021 tttgtcatct ggagccatgg aacagggttc atcatgagtc atcaacttac cttcgccgac
    10081 agtgaattca gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag
    10141 cagattctgc catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt
    10201 aatggccggc gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg
    10261 tacaacctga gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg
    10321 tttgcccggt tatccctgga tagcgccttg cctgaccgca ccaccatcat gaatttccgc
    10381 cacctgctgg agcagcatca actggcccgc caattgttca agaccatcaa tcgctggctg
    10441 gccgaagcag gcgtcatgat gactcaaggc accttggtcg atgccaccat cattgaggca
    10501 cccagctcga ccaagaacaa agagcagcaa cgcgatccgg agatgcatca gaccaagaaa
    10561 ggcaatcagt ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg
    10621 acccacagcc tggtcaccac cgcggccaac gagcatgacc tcaatcagct gggtaatctg
    10681 ct
//
