LOCUS       NZ_CP128488             4822 bp    DNA     circular CON 01-JUL-2023
DEFINITION  Klebsiella pneumoniae strain YZEF2-2C plasmid pYZEF2-2C-5, complete
            sequence.
ACCESSION   NZ_CP128488
VERSION     NZ_CP128488.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35779361
            Assembly: GCF_030369655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Wang,Q. and Li,R.
  TITLE     Distribution and transmission of the tigecycline resistant gene
            cluster tmexCD-toprJ in Klebsiella pneumoniae from animal feces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Wang,Q. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2023) College of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/29/2023 10:05:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,653
            CDSs (total)                      :: 5,530
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 73 of 170
            Pseudo Genes (incomplete)         :: 100 of 170
            Pseudo Genes (internal stop)      :: 32 of 170
            Pseudo Genes (multiple problems)  :: 30 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128488.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="YZEF2-2C"
                     /isolation_source="Laying hen"
                     /db_xref="taxon:573"
                     /plasmid="pYZEF2-2C-5"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.24 N 119.26 E"
                     /collection_date="2022-11"
                     /collected_by="Qiaojun Wang"
     gene            complement(join(4574..4822,1..120))
                     /locus_tag="QTN20_RS27895"
                     /old_locus_tag="QTN20_27895"
     CDS             complement(join(4574..4822,1..120))
                     /locus_tag="QTN20_RS27895"
                     /old_locus_tag="QTN20_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290306.1"
                     /translation="MKTTSEFARDAQKWDDRELGASENHVEVASMADMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAERKQALRRELDKLNEREEIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            complement(317..610)
                     /locus_tag="QTN20_RS27900"
                     /old_locus_tag="QTN20_27900"
     CDS             complement(317..610)
                     /locus_tag="QTN20_RS27900"
                     /old_locus_tag="QTN20_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290307.1"
                     /translation="MTIVISKEIEEKLTTKHKVTKKEVHESLANLNGNLLIDNREQHKT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNEIERKIYATHGKKTT"
                     /name="hypothetical protein"
     gene            1743..1934
                     /locus_tag="QTN20_RS27905"
                     /old_locus_tag="QTN20_27905"
     CDS             1743..1934
                     /locus_tag="QTN20_RS27905"
                     /old_locus_tag="QTN20_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1934..2335
                     /locus_tag="QTN20_RS27910"
                     /old_locus_tag="QTN20_27910"
     CDS             1934..2335
                     /locus_tag="QTN20_RS27910"
                     /old_locus_tag="QTN20_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064175096.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3063..3326
                     /locus_tag="QTN20_RS27915"
                     /old_locus_tag="QTN20_27915"
     CDS             3063..3326
                     /locus_tag="QTN20_RS27915"
                     /old_locus_tag="QTN20_27915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290305.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLARQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYSMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     gene            complement(4057..4320)
                     /locus_tag="QTN20_RS27920"
                     /old_locus_tag="QTN20_27920"
     CDS             complement(4057..4320)
                     /locus_tag="QTN20_RS27920"
                     /old_locus_tag="QTN20_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072072086.1"
                     /translation="MAAHTSETVKFLQSVSSDQTWWKSRYNPGDSVPYSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(4390..4518)
                     /locus_tag="QTN20_RS27925"
                     /old_locus_tag="QTN20_27925"
     CDS             complement(4390..4518)
                     /locus_tag="QTN20_RS27925"
                     /old_locus_tag="QTN20_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072201952.1"
                     /translation="MSLKPGPKRIADSTGQPDRRQRDNKKTPGNTDKLKPSKSSSK"
                     /name="hypothetical protein"
     Typing          complement(1220..1695)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 tgcatcgttg aaagctgaca tgtcagccat cgatgctact tctacgtgat tctcagaggc
       61 gccgagctct cgatcatccc atttttgtgc atcacgtgcg aattcactgg tagttttcat
      121 tgtatagacc tctaggtgct gtttcctgca gatcaggatt aactgcaata tttatgcttt
      181 atctagttcc gaaaggaact tctatgttga gaaatcaggt tcagcttcat tttcatttta
      241 gttctttcgc tgatgtaaga tttttgcaag gtcttacatg gtaaggcctt gcaaaacaat
      301 tacttattta gcgctgttat gtcgtctttt ttccatgcgt agcatagatt ttacgttcta
      361 tttcgttcgg ttcgaaggct gttttgatat gaatgtcgcc atcgataatc atgaaacaca
      421 ctttcaactc tctacgctgg ttagtttcag cgataaacca gttggttgga ggattggttt
      481 tgtgttgttc tctgttatct atcaacaggt taccgttaag gtttgcaagg ctttcatgta
      541 cttctttttt agttacttta tgttttgtgg ttagtttttc ttcaatttct ttactgatta
      601 caattgtcat atttcatgat gcctcggcct ttgttgtgaa attgaattca ttgtatatac
      661 aaaatttaca tttgtcaatc catggcttga ttgttaaggt gttttcattt tctatgtgta
      721 tatacatagg ctgtttcgat tgcttcttcg cagcctttgg tccgttgata ttggttatga
      781 aagatagata tagaagaagt tatgagcaat acaatagcat tgcctcattc atgtcattgt
      841 tctgactatg attatcgatt gaacccagtc gtagctttga cctatcaaga ggttgacgct
      901 ctatttggtt ttagaaatct acgcggcaac gtagctcctc agacgtggtg tagagtttgt
      961 cgtgttaaag gtgctgcaga tagcaaaaag aaaaaggcac aatcttaggc tgaggatctt
     1021 cttgagatca tgtgttagtc gtcgtaatct cactgctgtg aaaacgaaaa aaccgccctg
     1081 caaggcggtt ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct
     1141 tggaggagca cagtcaccaa aactgttcgt ttagtttagc cttaaccggc gcataagttc
     1201 aagactatct cctctaaatc agttaccagt ggctgctgcc agtggcgctt ttgcatgtct
     1261 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcagtcgg actgaacggg
     1321 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga gtgtcaggcg
     1381 tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     1441 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg
     1501 gtttcgccac cgctgatttg agcgtccgtt tctgtgatgc ttgtcagggg ggcggagcct
     1561 atggaaaaac ggcaacgccg tggccttttc cacaggctct tttcttcctg gttatcgcga
     1621 tgactcttct gtgtgtcccg tccgctcgcc gcagccgaac ggcaggagcg tagcgacgga
     1681 gtgagcgagg aagcggaaaa gagaacatcc ctacactgag cactgacgcc ctgaggcccc
     1741 ttatgacgaa acaacagaaa actgccctga acatggctaa attcatccag aatcagtctc
     1801 tgctcctgct ggagaagctt aacgagcttg atctcgatgt cgaggccgac ctttgtgaga
     1861 aactccacga tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgcattac
     1921 aggatggtaa ctgatggcca gagtgtctat tagtgaggct gcccgactga cgggtaagag
     1981 caggacaaca ctgcacaggt taattaaaac aggtgacctg tccacctgta gtggtgagcg
     2041 caatgcaaaa atggtagata cttcagagct tttacgtgtc tttggtccct ttgagcaacc
     2101 gaagggtgaa caccattctg gacaggtctc tgaacagcgt gatacagcct ctgctgacca
     2161 gagtgaacag gtgattcgtc atctgaaaca ggagattgaa cacctgaaca ccttactttt
     2221 gtccaaggat tcgcacattg acagcctgaa acaggccatg ctccttcttg aaaatcacca
     2281 ggcaaaatta actgaatccc cgacgccatg gtggaaattc tggaaaaaat cctgaccccg
     2341 tcatgcttca gttttcctct ccggccatcc cggggagttc ctgaaccttc ccaaaaatcg
     2401 tcagatctct gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaaaatt
     2461 ttgcagattc cttatttaca acaatgggtt aatgaaaaat aaatgtgcgg tgatgtgtta
     2521 caaagtgcag atccaagtcg tggacaactc tatggcgaat cgctgacgaa ttgcccacga
     2581 ttcatatgtg aatcacgctt tattatcata ttgttatgat gaatcgactc ttgattcaat
     2641 tcatcactct gggttttttt tgttcatggt gactcttgat tcaattcacg attcgaatcg
     2701 ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc cggccgtcga
     2761 cgaacccgat tcctgaacgt gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac
     2821 ggccacgcaa taaattgcgt ccccgttcac cgttttcgga taggaagaac gccggaaaca
     2881 ggacttaccc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg catactcatg
     2941 catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt
     3001 tttaccggtt tcccgtcaga aacgccttga ggccgttttt gcggtgcgta caagaagacg
     3061 ttatggtaaa tctaaatgat atctgcagaa gcaaaaaagc cgcctggata ggcggctttt
     3121 tcattttttt ggcgcggcag aacggcattt tcgacaccaa ctctggttgg ttgctcctga
     3181 gggtacagtt cttaagccga acagtgctga gagctgtcct gctgtatttg cagttgttac
     3241 gttacagcat gggcatgtat gaggcagacc gaggtttcca gacattcttc atcactccat
     3301 ttttttacca acaaacgtgg atatgactgc acccaaggcg atcatgccta atgtgccgac
     3361 tccgccgcca atacctgatg tacatgacgg acacaactca tcgtcataca aacgaccacc
     3421 cttagcgtaa ttctcacacc ccggagcgcg acatatgtaa attgcttctt tgcactcagg
     3481 gcattcgcac ttatctcgtc gtacatggtt ttcttgaatt acacgaggta taacttcagc
     3541 tttacaaaaa ggacataacc ctttgtgttt tgatgtcatt ttgacctact taattgcact
     3601 gcaactaatt aattttgaac gacatttagg gcagtagttc ggtgttccaa acccactcct
     3661 actagtccat tcatgccgac aagccatgca tttagcccga tagataacaa caaccgccat
     3721 gaaaaaccac cattaatatc agtaactaat attcatttat aataactgac aaactaaaca
     3781 agtaaagtaa attgtgtatt tttttaagtg attgattttt aatttatttt tctattatct
     3841 aaaggctgta aattttaatt aatgcattaa atacaatgac ttaatctgat ttgttttttt
     3901 tgtttaactt ttttggaggg cgagaaagac tcatgcatct cgttcgtgcg ctcatgcagc
     3961 gtaacgattg ttagcataca agaagtgcgg ggctgggatg ttatggtaaa tatcttcgag
     4021 cttcaggtgc aaaaaaaagc cccggagggc tctcaatcat ttgattccga aacggtcacc
     4081 tttagtgtcc gttctgacaa tcaattgcca ttttattgct tgttgctgac tgtgctgatg
     4141 gtggttttgt ggtgggaacg ggtcgctgcc gttactggtg atctcttttc cgcaaactgt
     4201 acatcgataa attccagaat agggaacgct gtctcctgga ttatatcgtg acttccacca
     4261 tgtttgatcg ctactgacgc tttgtaaaaa cttcactgtt tcgctggtat gtgcagccat
     4321 ttagctttcc ttttctgaat ttataggact gacaatgaaa agccgcctaa aaggcggctt
     4381 ttaactgatt catttacttg aggatttact gggttttaat ttatcagtat ttcccggtgt
     4441 ttttttattg tcacgttggc gtctatcagg ttgccctgtt gaatctgcga ttctttttgg
     4501 accaggttta agactcatga taatcctcct ttcaaacgcg atgaaatttt ttctatctag
     4561 atggtaagct ctttcatgct tgttttctta tgctttcaat gaattcatca actggaactg
     4621 tgtcctcctg ctgatgtatt tcttctcgtt cattgagctt gtccaactct ctacgtaatg
     4681 cttgttttct ttctgcaata acaaatcgtt gcagaaggtc tctaaccatt ggttgataac
     4741 ctataccata gctttcagct atcgatttta aatcgcttac aagatttttc tgtaatctta
     4801 ttgagatggc ttgcagtgat ag
//
