LOCUS       NZ_CP128462             5141 bp    DNA     circular CON 03-JUL-2023
DEFINITION  Enterococcus faecalis strain RE25 plasmid pVPRE25a, complete
            sequence.
ACCESSION   NZ_CP128462
VERSION     NZ_CP128462.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35741683
            Assembly: GCF_030376825.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Perreten,V., Collaud,A. and Teuber,M.
  TITLE     Complete genome of Enterococcus faecalis RE25 from dry sausage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Perreten,V., Collaud,A. and Teuber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-JUN-2023
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: RE25.fasta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 268.0x
            Sequencing Technology             :: Oxford Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/01/2023 07:10:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,894
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 24 of 60
            Pseudo Genes (incomplete)         :: 23 of 60
            Pseudo Genes (internal stop)      :: 20 of 60
            Pseudo Genes (multiple problems)  :: 7 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128462.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="RE25"
                     /isolation_source="dry sausage"
                     /db_xref="taxon:1351"
                     /plasmid="pVPRE25a"
                     /geo_loc_name="Switzerland"
                     /collection_date="1991"
     gene            1..723
                     /locus_tag="QSV41_RS00030"
                     /old_locus_tag="QSV41_00030"
     CDS             1..723
                     /locus_tag="QSV41_RS00030"
                     /old_locus_tag="QSV41_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            850..1146
                     /locus_tag="QSV41_RS00035"
                     /old_locus_tag="QSV41_00035"
     CDS             850..1146
                     /locus_tag="QSV41_RS00035"
                     /old_locus_tag="QSV41_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            1922..3601
                     /gene="mobV"
                     /locus_tag="QSV41_RS00040"
                     /old_locus_tag="QSV41_00040"
     CDS             1922..3601
                     /gene="mobV"
                     /locus_tag="QSV41_RS00040"
                     /old_locus_tag="QSV41_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_289690431.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQKNTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            3690..3890
                     /locus_tag="QSV41_RS00045"
                     /old_locus_tag="QSV41_00045"
     CDS             3690..3890
                     /locus_tag="QSV41_RS00045"
                     /old_locus_tag="QSV41_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(4409..4495)
                     /locus_tag="QSV41_RS00050"
                     /old_locus_tag="QSV41_00050"
     CDS             complement(4409..4495)
                     /locus_tag="QSV41_RS00050"
                     /old_locus_tag="QSV41_00050"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          complement(1379..1638)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          2..723
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1866..3598
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.423"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgata aatcggaatt agctgttaaa tatcaaaatg agttaaatct tgtgccactt
       61 aaaaacttta atgcaaaaca aatggatctg ttctttgcgt tatgcgcacg aatgaaagat
      121 aaaggagtag agaatattcg tttctccttc gaggaattaa aggaattgag tgactacaaa
      181 atgactgcta caaaagcctt tgtagcagac ttagaaaagt tgtataaaga tatgctgaac
      241 ctttcttatc gtacagaaaa cgatgacgaa atagagtatt ttgttttatt caatggattt
      301 aaaatcgaca aaaaacaaaa gtttgttgaa gttcgagtta atcaagattt agactatata
      361 ataaatggtt taacaacaga gtttagtagg tttgagctgt ccgcttttac ttcaatacgc
      421 tcaacatatg ctaaaacatt gtttcgtttg ttaatgcaat atcgttcaac agggtattat
      481 gtagtcagta ttgaagactt cagagaattg ttagacatac ccgaatatta tcaaatgggt
      541 aacattgacc aaaaggtttt aaaaccagca atgaaagaac tccataacta ttttgagaat
      601 ttagaagtaa caaaaatcaa agccaaaaaa ggcaataaga ttgccaaact agaatttacg
      661 tttactggtc tgaaaaccaa caaaccaagc gtaaccatgc acgattgggt aaacggagaa
      721 taaaaaaaga acttgccccc tcacattaag caagttagca agttcctcac gcaatcttcc
      781 ggactgctag attattttag catatttgcg tgaggacttc taaaataaat tattttggag
      841 gtcctactta tgggattaat gacaggatac aacatgacaa aagaggagta cgaagtcaca
      901 caggctgtac tggagcgact agataaattt gagagtgaac tagaagcgct attgcttgat
      961 ttggagccac ttgacttatc acgattagat actgtagttc gagacgaaac gctaagaatg
     1021 tttaattatc taagtcggtc atacattcta cttattgata cggtagccta caaagttacg
     1081 ctagaagaag atatttcctg tgggattgtg aagattgacg gtcaatctct agacgaagaa
     1141 gaataaaaac tacccttgct tgttataaaa aaatgttgac tatttgggat aacactcgaa
     1201 aaatatcaag attagcctac ttccattttg gggtaggctt tctttttgca agcggagcaa
     1261 catggtacca tgttgcgata ttttgaacga ctcctaccaa aaatactggt aggagtcgtt
     1321 caaatttttg gggttccccc aatcccactg ctgttatttt ttgcttttac tgttgaaatt
     1381 cgcttaattt cttcctgtgt ttcggaagag cataagggga actggggggc acacaaggta
     1441 ccccgccttg cagtgcaagg ctttcccgtg gggtacactt tcgttcttcg ctagcacttt
     1501 actagcaggt actttcaggt aaactagcac atttttagca ctcaatagca ggtttctagc
     1561 acccaaacta gcagattcct agcagtgact agcaggtttt tagcaccaaa ttagcacatt
     1621 actagcaccg gctagcagaa agctagcacg ttcactaatt tcaggcagtc gttatttaaa
     1681 cggttttctg acggagggaa atttggagtt tctgccttag cagaaatgga aagtttgacc
     1741 gtaggcaatc ggctaagtgc gagattggga agcctaaagg ctgacctttc gagtactagc
     1801 cgacagccct cggcagagcg cacctttacg aagtaaagta tagtgcgtta tactttactt
     1861 ggaagtactg ccgaaatcca aagacaccga acgacacaag aaaaaaaggg ggggagcgct
     1921 catgtcaaaa attgttgcac gtatggaaaa aatgaaagac ggaaatttaa gtggtatcca
     1981 acgacataat caacgagaaa ccaataatca ttccaatcct gatattgata ttgagaaatc
     2041 tcacttgaat tatgacttag tcaatcctgg ttcaatcaat tatcgggaga aaatcaaaca
     2101 aatcattgag agccaacgaa tcagtaaacg agcggttaga aaagacgcag tccttgtgaa
     2161 cgaatggata atcactagtg ataccgcctt ttttcaaaag aatacagaca cacaagcatt
     2221 ttttaccgat gttgtcgcgt atttctctga tcgctgcggt cgacaaaatg tcgcctatgc
     2281 cacggtacat ttagacgaaa ccacgcccca tatgcactta ggaattgtgc ctatgtacga
     2341 agggcgattg agcagtaaac aggtgtttag tcggcaaaat ttgttagaga ttcaagaaga
     2401 attgccaacc tatttgcaag aacgaggcta tgctattgag cgtggactcc gtgggagtcc
     2461 gcaaaagcat ttatctgtca aagaatacaa agacgttcaa aaggagctac aagtttctaa
     2521 acaggaactc gctaatcagg aacaaattct ggcagaaaaa gagagagaac tccattctta
     2581 tgaacaggca atcttccgta ttgaccgcct tcttgaagag aagaacgaag aagtaaaggg
     2641 caaagtccaa gcaatgaacc aactggaaac gaagcgaaac gaaatcatta actctctaca
     2701 aacaaccgtt ttacctgatt tagcaaatgt caaagaaaag aaatttagtt taaacggttt
     2761 aaaatacgtt ttaagcgctt ctgacttttc ggcgataaaa agtattacga aagatttcga
     2821 gacgcttaga acgcaaaata agcaccttaa aatcgaaaac aataacttgg cacagaaaaa
     2881 taaagaattg acacaagtaa atcatgaaca agaattacaa ataacggaat tagcctatga
     2941 attaaccctg aaaaaagaac aattaacagc gtttgaagaa aaacaactaa actatgacca
     3001 gagcgaacaa atcaaacaag aacttgatac aacaacacaa acagtttctg tgttagaaac
     3061 agagaataag cggttaaaag ataaaattac tgacttaacc tacgactttt ctgaactaaa
     3121 tgaaaaatat caagaaataa aattaaagtt tgacgggtta actacttacc ttcacgaaag
     3181 actaggtcat gctaaagaaa ccattttatt tacgattcag caaggagtga agtaccttca
     3241 gaacgcgcct aaattaaaaa aatccctaga gcgatattcc aaggatagaa cttacctcat
     3301 tgacgaaaaa gggacattgt ttttaccacc agatacgggg tcaaaagggg taacaatgtc
     3361 cttaaaccca catttctacc ccctaaaagc cccgttttac aacagagaaa cagggtgtta
     3421 tgagtttgtc ggcgaatata tggctaatgg acgttctcga acgaatactt tacaactgtc
     3481 tgaagaacaa gtgctagaga agaaagcttt tacgtttgaa cagtcgacag ataaaaaggc
     3541 ttggcaacag agtaaaaaat attcaagaga tatgtcaaaa ggacgaggtt tatctcgtta
     3601 aaaataattt aattgataaa ttattgacac ataaaaacag ttaaattaaa atatattaat
     3661 aaaaattaaa aaaaagtgag gtaagcatta tgaaaaagtt gttagttcta attttagggg
     3721 tgtcttttat ctctgttgct ttaagttcaa ctacagaagc aagtgctaga gagtgggatt
     3781 ctatagagtg tcagccagga ttttattgga aaaaaggcgg gtggactgca ggaggatatc
     3841 ataaagccgg tggattttgt atcccaatgc atacaagatg gacaaaatag ttgctaaatt
     3901 tgaggttatt tcatgaaaaa atatctaata aaaaatatat tttatattac tattccttta
     3961 gttttaagct atataacttc ttttttagtt aatattgatt tgcccatatt gataattatt
     4021 ttttacggaa ttttattatt ttttttaatt ccttcagaag tatatttggg aagtacaatg
     4081 gattacaacg cgaaagtagt gaatcccact tacagaccag aaaataaaag ctttgaagat
     4141 agtccaaaaa gtaaaatatt gtctatcctc atagtattac tatgtttaat actaactatt
     4201 tcgatatggt atttctctaa ttgacgtttt gcgtaatcta atctgatgtt tgaaaataca
     4261 gaaaaaaaat gttattgtgt aagtgcgaag cgaaattaag caaaatcatg tttacaccaa
     4321 aaagccctcc tattctcagt aggagggctt ttttacactg ttgcgatttc agcgttggag
     4381 ctagaaaagc aactgcatag attatctgct aatcgtctag ccaacgatca actagcaaaa
     4441 gtactaaacc cacaaaaagt ggggcgataa tcaacgaaat taagctttca atcatgttta
     4501 cacccccttt cggggcagtt gccaaaacaa gtatatcaca agaagaattt cttgtggggg
     4561 ttgttagggc ttgccctgac cgcctgcaag gcgcaatcct aaccaagata ccctaaaaca
     4621 actaaacatg tcttaaagat cttcagacaa gcaaatagag cctaaccaaa tgatttacct
     4681 tgtcaacggt attgattttg attttagcga gtgaacgaag tgaacgctgc aagcaaaatg
     4741 tgagcgggat tttcgctcac tccttttttg attttttagt gccagttttg taaatatcgc
     4801 ggacctgttt gtagaaaaaa gaggttaatt ttgggcggga aatttccgcg cgcgcacgcg
     4861 tttcttttat atatatttta tatatatttt taaaaccttt ttaggcgggc tgtgtgccac
     4921 gtggctctaa ggaagagcag atggtaccaa caacttttta tctgactacc aacaactttt
     4981 tatctgacta ccaacaactt tttatctgac taccaacaac tttttatctg actaccaaca
     5041 actttttatc tgttttattt tcataatatt aaaacaaaca accaactgtt gacaaagttg
     5101 gttgtttgtt ttaatattcg aattgtgagg gggcgaaagg a
//
