LOCUS       NZ_CP128461             4802 bp    DNA     circular CON 03-JUL-2023
DEFINITION  Enterococcus faecalis strain RE25 plasmid pVPRE25b, complete
            sequence.
ACCESSION   NZ_CP128461
VERSION     NZ_CP128461.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35741683
            Assembly: GCF_030376825.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Perreten,V., Collaud,A. and Teuber,M.
  TITLE     Complete genome of Enterococcus faecalis RE25 from dry sausage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Perreten,V., Collaud,A. and Teuber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-JUN-2023
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: RE25.fasta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 268.0x
            Sequencing Technology             :: Oxford Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/01/2023 07:10:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,894
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 24 of 60
            Pseudo Genes (incomplete)         :: 23 of 60
            Pseudo Genes (internal stop)      :: 20 of 60
            Pseudo Genes (multiple problems)  :: 7 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128461.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="RE25"
                     /isolation_source="dry sausage"
                     /db_xref="taxon:1351"
                     /plasmid="pVPRE25b"
                     /geo_loc_name="Switzerland"
                     /collection_date="1991"
     gene            join(3427..4802,1..304)
                     /gene="mobV"
                     /locus_tag="QSV41_RS00005"
                     /old_locus_tag="QSV41_00005"
     CDS             join(3427..4802,1..304)
                     /gene="mobV"
                     /locus_tag="QSV41_RS00005"
                     /old_locus_tag="QSV41_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_174104218.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQKNTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPNLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYE
                     LTLEKEQLTAFEEKQLNYDQSEQIKQELDKTTQKVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGAKGVTMSLNPHFYPLKSPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLENKAFTFEQSTDRKVWQESKNHSKGLSKNRGLSR"
                     /name="mobV"
     gene            425..625
                     /locus_tag="QSV41_RS00010"
                     /old_locus_tag="QSV41_00010"
     CDS             425..625
                     /locus_tag="QSV41_RS00010"
                     /old_locus_tag="QSV41_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="WP_142966541.1"
                     /translation="MNKYKELSEKELKQTIGGGWGDFLGGLLGGLAPTRTAEQLNGSDY
                     ANFKARNCGPYGQGGTPNSCN"
                     /name="bacteriocin"
     gene            649..966
                     /locus_tag="QSV41_RS00015"
                     /old_locus_tag="QSV41_00015"
     CDS             649..966
                     /locus_tag="QSV41_RS00015"
                     /old_locus_tag="QSV41_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016388709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033922266.1"
                     /translation="MKTLYKKKLADVWEISKTNEQPEWVKQAFAKNYLRWTTDNRLVVL
                     MSALEPSVITNIKFGMVGSVGGGFAGYAMYSVGDIGDFLDATNHKIVSKKRFHKQYVIQ
                     G"
                     /name="hypothetical protein"
     gene            1534..2271
                     /locus_tag="QSV41_RS00020"
                     /old_locus_tag="QSV41_00020"
     CDS             1534..2271
                     /locus_tag="QSV41_RS00020"
                     /old_locus_tag="QSV41_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_142966542.1"
                     /translation="MKEKTELAVKYQNKLNLVPLKNFNSKEMDLFFALCARMKDRGTEE
                     ISFTFEELRNLSDYKMTATKAFIKDLDSMYSKMLQLTYRENLFDDDDDGFRRFVLFTGF
                     EVSTKQQVVKVSVNSKLESVINGLTTEFSKFELAAFTSIRSSYAKTLFRLLMQYRSTGY
                     YQVNIEEFKEIMDVPKSYTQMGQIDQKVLKPAMKELHNYFEDLEITKIKAKKGNKIAKL
                     EFTFTGLKTNKPSITMHDWVNGE"
                     /name="replication initiation protein"
     gene            2400..2696
                     /locus_tag="QSV41_RS00025"
                     /old_locus_tag="QSV41_00025"
     CDS             2400..2696
                     /locus_tag="QSV41_RS00025"
                     /old_locus_tag="QSV41_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033922262.1"
                     /translation="MGVMTGYNMTKEEYEVTQAVLTRLNKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLLDTVVYKDTLEEDIACGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     Typing          3371..4800
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.634"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cattgacgaa aaagggacat tgtttttacc accagatacg ggggcaaaag gggtaacaat
       61 gtccttgaac ccacatttct accccttaaa atccccgttt tacaacagag aaacagggtg
      121 ctatgagttt gtcggcgagt atatggcaaa tggccgttct cgaacgaata ctttgcaact
      181 gtctgaagaa caagtcctag agaataaggc ttttacgttt gaacaatcaa cggatagaaa
      241 ggtttggcaa gaaagtaaaa accattcaaa aggactgtca aaaaaccgag gcttatcccg
      301 ataattgttt tagtaagtat aataactcgt gtcacctata gatatctttt gacgactcta
      361 attttcacat gataatcttg aaatgaatca aaaaattcat acaaaaagaa aggcggtaaa
      421 ttcaatgaac aagtataaag aattgtcaga gaaagaatta aaacaaacca ttggcggcgg
      481 atggggcgac tttttaggag gccttttagg aggattagct cctactcgta ctgctgaaca
      541 gttaaatggt tcagattatg caaattttaa agctcgtaat tgtggtcctt acggacaagg
      601 tggcacgcct aactcttgta actaataaga aagcgctggt ggtttactat gaaaacttta
      661 tataaaaaaa agttagctga tgtgtgggaa attagtaaaa ctaacgaaca gccagaatgg
      721 gtcaaacaag ctttcgctaa aaattatcta agatggacta ctgataatag actagtcgtt
      781 ttaatgagtg cgttagaacc ttcagtgata acaaatataa aatttggaat ggttggttct
      841 gttggtggtg gttttgctgg ttatgcaatg tattctgtag gcgatatagg cgatttttta
      901 gatgctacta accataaaat tgtgtcaaaa aaaagatttc ataaacaata tgtaattcag
      961 ggttgaaaaa ggttttcggg ttgttagggc ttgccctgac cgcctgaaag gcgccagcct
     1021 aacaagatta taaaaaacta ctaaaaatgt cttagagagc cttagataag caaatagagc
     1081 ctatccaaag agtaccttat caatggtttt gactttggtt ttagcgagtg aacgaagtga
     1141 acgctgcaag taaaatgtga gcgtgatttt cgctcactct ttttttgtct tttgattttt
     1201 taatgacagt tttgtaaata tcgcgaacct gtttgtagaa aaaagagaag aattttaggc
     1261 gggaaatttc ctcgcgcgcg tacgcgttta ttttatatat attttatata tatttttaaa
     1321 aaccttttag gcgcgctgtg tgctatgggg ctctaaggga tagcagaggg taccaacacc
     1381 tttttatctg actaccaaca cctttttatc tgactatcaa caccttttta tctgactacc
     1441 aacacctttt tatctgtttc ctataacaaa cactacctat tgactatgca ggtagtgttt
     1501 gttatagtga tatctgtgag gggggcgaaa ggaatgaaag aaaaaacgga attagctgtt
     1561 aagtatcaaa ataaattaaa cttagtgcct ttgaaaaatt tcaactccaa agaaatggat
     1621 ttattttttg ctttatgtgc acgaatgaaa gatcgtggaa ctgaagagat ctcatttact
     1681 tttgaagaac ttcggaatct tagtgactac aagatgactg ctactaaagc ttttataaag
     1741 gatttagaca gcatgtacag taaaatgtta caactaactt accgagaaaa tttatttgat
     1801 gacgatgatg acggattcag acgttttgtt ttatttacag gctttgaggt aagtacaaaa
     1861 cagcaagtag ttaaagttag cgtaaattct aaacttgaat cagttataaa tggcttaact
     1921 accgaattta gtaagtttga acttgcagcc tttacgtcga ttcgttcaag ttatgcaaaa
     1981 acattgttcc gtttgcttat gcagtatcgc tctacaggat attaccaagt aaatattgag
     2041 gaattcaagg aaataatgga cgttccaaaa tcgtacacac aaatgggcca aattgaccaa
     2101 aaggttttaa aaccagcaat gaaagaactc cataactact ttgaagattt agaaataaca
     2161 aaaatcaaag caaaaaaagg caataagatt gccaaactag aatttacgtt tactggtctg
     2221 aaaaccaaca aaccaagcat aactatgcat gactgggtaa acggagaata aaaaaagaac
     2281 ttgcccccct cacattaagc aagttagcaa gttcctcacg caatccacta cgactgctag
     2341 attattttag catatttgcg tgaggacttc taaaataaat tattttggag gtcatacaca
     2401 tgggagtaat gacaggatac aacatgacaa aagaggagta cgaagtcaca caggctgtac
     2461 tgactcgact aaataagttt gagagtgaac tagaagcgct attgcttgat ttggagccgc
     2521 ttgacttatc acgattagat actgtagttc gagacgaaac cctaagaatg tttaattatt
     2581 taagtcggtc gtacattcta cttcttgata cggtcgtcta caaagatacg ctagaagaag
     2641 atatcgcctg tgggatcgtg aagattgacg gtcaatcact agacgaagaa gaataaaaac
     2701 tagccttgct tgttataaaa aatgttgact atttgggata acactcgaaa aatatcaaga
     2761 ttagcctact tccattttgg ggtaggcttt ctttttgcaa gcggagcaac atggtaccat
     2821 gttgcgatat tttgaacgac ccctaccaaa aatactggta ggagtcgttc aaatttttgg
     2881 ggttccccca atcccactgc tgttattttt tgcttttact gttgaaattc gcttaatttc
     2941 ttcctgtgtt tcggaagagc ataagggaaa ctgggtccca cacaaggtgg gacgccttgc
     3001 agtgcaaggc tttcccgtgt ggtacacttt cgttcttcgc tagcacttta ctagcagtta
     3061 ctttcaggta aactagcaca tttttagcac tcactagcag gtttctagca ccaaattagc
     3121 acattactag caccagctag cagaaagcta gcacgctatc tattctcagt cagtcgttat
     3181 ttaaaagttt ttttgacgga gggaaatttg gagtttctgc cttagcagaa atggaaagtt
     3241 tgaccgtagg caatcggcta agtgcgagat tgggaagcct aaaggctgac ctttcgagta
     3301 ctagccgaca gccctcggca gagcgcacct ttacgaagta aagtatagtg cgttatactt
     3361 tacttggaag tactgccgaa atccaaagac accgaacgac acaagaaaaa aaggggggga
     3421 gcgctcatgt caaaaattgt tgcacgtatg gaaaaaatga aagacggaaa tttaagtggt
     3481 atccaacgac ataatcaacg agaaaccaat aatcattcca atcctgatat tgatattgag
     3541 aaatctcact tgaattatga cttagtcaat cctggttcaa tcaattatcg ggagaaaatc
     3601 aaacaaatca ttgagagcca acgaatcagt aaacgagcgg ttagaaaaga cgcagtcctt
     3661 gtgaacgaat ggataatcac tagtgatacc gccttttttc aaaagaatac agacacacaa
     3721 gcatttttta ccgatgttgt cgcgtatttc tctgatcgct gcggtcgaca aaatgtcgcc
     3781 tatgccacgg tacatttaga cgaaaccacg ccccatatgc acttaggaat tgtgcctatg
     3841 tacgaagggc gattgagcag taaacaggtg tttagtcggc aaaatttgtt agagattcaa
     3901 gaagaattgc caacctattt gcaagaacga ggctatgcta ttgagcgtgg actccgtggg
     3961 agtccgcaaa agcatttatc tgtcaaagaa tacaaagacg ttcaaaagga gctacaagtt
     4021 tctaaacagg aactcgctaa tcaggaacaa attctggcag aaaaagagag agaactccat
     4081 tcttatgaac aggcaatctt ccgtattgac cgccttcttg aagagaagaa cgaagaagta
     4141 aagggcaaag tccaagcaat gaaccaactg gaaacgaagc gaaacgaaat cattaactct
     4201 ctacaaacaa ccgttttacc taatttagca aatgtcaaag aaaagaaatt tagtttaaac
     4261 ggtttaaaat acgttttaag cgcttctgac ttttcggcga taaaaagtat tactaaagat
     4321 ttcgagacgc ttagaacgca aaataagcac cttaaaatcg aaaacaatac cttggcacag
     4381 aaaaataaag aattgacaca agtaaatcat gaacaagaat tacaaataac ggacttagcc
     4441 tatgaattaa ccctggaaaa agaacaatta acagcgtttg aagaaaaaca actaaactat
     4501 gaccagagcg aacaaatcaa acaagaactt gataaaacaa cacaaaaagt ttctgtgtta
     4561 gaaacagaga ataagcggtt aaaagataaa attactgact taacctacga cttttctgaa
     4621 ctaaacgaaa aatatcaaga aataaaatta aagtttgacg ggttaactac ttaccttcat
     4681 gaaagactag gtcatgctaa agaaaccatt ttatttacga ttcagcaagg agttaagtac
     4741 cttcagaacg cgcctaaatt aaaaaaatcc ctggagcgat attccaagga cagaacctac
     4801 ct
//
