LOCUS       NZ_CP127319            11176 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Escherichia coli strain Ch1 plasmid pCh104, complete sequence.
ACCESSION   NZ_CP127319
VERSION     NZ_CP127319.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35637910
            Assembly: GCF_030285585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Complete Genome Sequences of Three Commensal and Two Avian
            Pathogenic Escherichia coli Strains Isolated from Farm Animals in
            Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11176)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Laboratory of Metagenome Analysis, Skolkovo
            Institute of Science and Technology, Bolshoy Boulevard 30, bld. 1,
            Moscow 121205, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2023
            Assembly Method                   :: Flye v. 2.9.1
            Assembly Name                     :: E_coli_Ch1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 17:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,591
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,077
            CDSs (with protein)               :: 5,077
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 1 of 390
            Pseudo Genes (frameshifted)       :: 206 of 390
            Pseudo Genes (incomplete)         :: 208 of 390
            Pseudo Genes (internal stop)      :: 86 of 390
            Pseudo Genes (multiple problems)  :: 92 of 390
            Pseudo Genes (short protein)      :: 4 of 390
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127319.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ch1"
                     /isolation_source="feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pCh104"
                     /geo_loc_name="Russia: Perm krai"
                     /collection_date="2020-07"
                     /collected_by="Kuznetsova Marina, Mihailovskaya Valentina"
     gene            217..918
                     /locus_tag="QRM71_RS27565"
                     /old_locus_tag="QRM71_27565"
     CDS             217..918
                     /locus_tag="QRM71_RS27565"
                     /old_locus_tag="QRM71_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2645..4804)
                     /gene="mobQ"
                     /locus_tag="QRM71_RS27570"
                     /old_locus_tag="QRM71_27570"
     CDS             complement(2645..4804)
                     /gene="mobQ"
                     /locus_tag="QRM71_RS27570"
                     /old_locus_tag="QRM71_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_072648256.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELQQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFETQWQQWGASGLAALREQL
                     RKREEELRRKEQEETERRRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFECVDLMRDRSGYICDRSEIVQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAIPSPAARKVDFMQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNYDADSQISPAPELMKFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRRDEQHRKQERYPGNHRGMSM"
                     /name="mobQ"
     gene            4983..5273
                     /locus_tag="QRM71_RS27575"
                     /old_locus_tag="QRM71_27575"
     CDS             4983..5273
                     /locus_tag="QRM71_RS27575"
                     /old_locus_tag="QRM71_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029490380.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            5805..6506
                     /locus_tag="QRM71_RS27580"
                     /old_locus_tag="QRM71_27580"
     CDS             5805..6506
                     /locus_tag="QRM71_RS27580"
                     /old_locus_tag="QRM71_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(8233..10392)
                     /gene="mobQ"
                     /locus_tag="QRM71_RS27585"
                     /old_locus_tag="QRM71_27585"
     CDS             complement(8233..10392)
                     /gene="mobQ"
                     /locus_tag="QRM71_RS27585"
                     /old_locus_tag="QRM71_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_072648256.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELQQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFETQWQQWGASGLAALREQL
                     RKREEELRRKEQEETERRRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFECVDLMRDRSGYICDRSEIVQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAIPSPAARKVDFMQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNYDADSQISPAPELMKFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRRDEQHRKQERYPGNHRGMSM"
                     /name="mobQ"
     gene            10571..10861
                     /locus_tag="QRM71_RS27590"
                     /old_locus_tag="QRM71_27590"
     CDS             10571..10861
                     /locus_tag="QRM71_RS27590"
                     /old_locus_tag="QRM71_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029490380.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     Typing          complement(4447..4805)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          4806..4982
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="1.22e-84"
     Typing          complement(10035..10393)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          10394..10570
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="1.22e-84"
     Typing          218..918
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.459"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5806..6506
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.316"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2652..4891)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.448"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8240..10479)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.448"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcttatg gaattcaata gtttagaaga ctataggtaa tataatttaa ggtaaaggca
       61 atatgattta aggtggatag gcaatatgat ttaaggtaaa ggcaatatga tttaaggtaa
      121 tatccacagg aaaagggata ttaaaattaa atcatattgc ctttacctaa gggaatgttt
      181 atattttact cattgcctta ggttgaggga tttacaatgt cggatttgat tgcctacaaa
      241 tccaatgcac tggttgaggc cagctataaa ttgactcttc aggaacagcg attcttgtta
      301 ctttgcattg gtcgattgaa gtcgggttct gatgcagaat ccactgaatt gcaaaagacc
      361 atgactgtta ccgccgaaga gtatttcgac tcattccctg atatggggcg gaaaaatgcc
      421 gaggttcagc tacaggaagc aattgatcgt ttgtgggaca ggtcaatcat ccttaaggac
      481 gatgagaagc gcgaggagtt ccgctggatc cagtatcggg cacagtacgc taaaggtgag
      541 ggcaaagccc aaatcacttt ttcagatgcg gtcatgcctt atctgacaca gttgaagggg
      601 caatttacta gagttgtaat taagaatatc tccaatttat caagaagtta cagcataaga
      661 atatacgaac ttctccagca attccgcagc acaggggaac gtattatcgc cctcgatgat
      721 tttcgctcaa tgcttgatat agagcataaa tatcaaacat ataagtctct gaaccagcaa
      781 ttattaagac cttgcgttga cgaactgaat caaaaaagcg acttggctgt aacgcttgag
      841 acgatcaaga aggggcgcac ggttgtagcc ctgcattttc gcttcaagga agacaaacag
      901 atcaagatga cgatctaaaa tcgattttaa ggcatctaaa acgccatctg gttcgttttt
      961 ttggtgtcag gtatgcgatg gtactaccaa gcaaataaat tgcgtcaaaa tcgattttag
     1021 agagcggtat ggcaaaccat gtcgaacatt acttgtttta cttgacgtga gagatgcgtt
     1081 aatgccgcca gcagcagtcc cccatcgaag tgactcatgc gtgactaaag agcaagcctg
     1141 agcaacgtag gaatctccaa ccttcaggac gggatggata tcaagcacca gggcacactt
     1201 gaaaacaaaa cctcgcctct ggcaaagcgc gtcctttcac gatgacgtag aggtatttga
     1261 atatctgtag gagttcatca cgctatttta acgcttaaaa agcgcgtttc tgattgtgta
     1321 actaaacaca ggtagtaatt agtaggtgtg gggggggcag aaacgcgcaa ttaatattta
     1381 tcgtgaattt gattttatgt ctagtatcga gaaacagaat ataaaaagat tatctttgga
     1441 aatatagaaa aaaaataaaa taataaacta aaaacaatca aaaaataata aatattacac
     1501 tgcaacatgg caacaatata ttcattcata tagtgatata agctatggtt ttcaaaattt
     1561 tccttgagct gaagaacgga gttttatggc aaacgaggta atcttgcaca ggaaaaatag
     1621 gttgtatcta tgtcatggtt ttgttatttt tttttgctca gttattgatg gtcaatatgt
     1681 aaaataaatc aggtacttcc ttacacttac cctcattcat tgagggtttt ttttatccat
     1741 atctaagttg acatcctcca tgccctgaag gacggggttt tacagcacac cggataacta
     1801 tctcgacatg gatattgata cacgttaaaa atacgtgagg cgtttctggc agatatctga
     1861 ggtctatttc gccgtgttag ccacactgtc agaaacgcgt tcaatttctc ccgttgagct
     1921 gatatgctca tcaccgcaat cacggtagta tccgaagacg aaccgttaat gcatcttccc
     1981 cccgatactg ggctttcggg gggaagattt actcggtgcg ccgtaaaacc ccgtccttca
     2041 gggcatggag gatgtcaaca cgaatcccct atcttctttt cagtaagaaa cggtcattga
     2101 aaacttatat aacactacat ttggtgtgta ttttgcgata aaaatatccc ctatcctaat
     2161 gatagggaat acaaacgatg aattaacagt tcttaattct cacgtgaaaa gatagatttt
     2221 aacgaagaca caatcagcag tcttatatcg aatggctatc aatcctgaat cctgatgagt
     2281 ggggatgttt ctgtactacc aatgaaaagc tatccggcta gagactttac gatacagaaa
     2341 cacccctctt tcttttatct catcagatgc catagcgcaa aacaatttcg tttcacagat
     2401 gtgacttaca atcaaactgt gcaacataaa tcacaaaaca agaaggtttt gttattattt
     2461 tgttgatgag ttgtttttcg ttggttgttt ttttgaggcg tacatacgcg cacaaatgta
     2521 aaactaaatt cgtgttttcg ataattttaa ggccacaatc attatggcat tttttatcct
     2581 gatcgccgtc aaaatctgtc cttcatggca gggtttacgg cgatccggat aacaccaaag
     2641 atacctacat gctcattccc ctgtggtttc caggatatct ttcctgtttt ctgtgctgtt
     2701 catcgcgccg ccattcgtcc caggtacgat taaaacccag agagcggaac agatgatccg
     2761 gctgtttttc tggtgtattg acggcagcaa aaagcggtgc ctcacgggtt ttcagatgaa
     2821 acttcataag ttccggtgca ggtgatatct ggctgtctgc atcgtaattg acggcaacac
     2881 cataccctgc ataccgtaac cccttgtcgg tcagcgcact gatgaccaga tgcctgttat
     2941 cggctgaggg aacggcaaac gcgggataca gcgtaccatt aagccggaca acaccgccca
     3001 gagagtcatc cacgcccatg accagcaaaa gctgaagctg gcgggtttcc cggtcgacag
     3061 aactcatgac cggcatcagc gtcggtggca gacgtccctg cataaaatcc accttccttg
     3121 ccgctggtga tggtatggct ggctggcggt cttctccaag acagaaagac tccggccatg
     3181 tcttcggctg gcggagattg cgggtatatt gcggccatgt ctggcaaacc tgcgtcagaa
     3241 gttgctcaaa ggtcgcagag gaataaacac gttgcagtgg tggatgttgg acgatttcgc
     3301 ttcggtcaca gatataacca ctcctgtcac gcattaaatc aacacactca aagattctgg
     3361 tgcggtattt ctccagctcg ttcccccaga gcgtatattc tccgttccgg ttgtgaagca
     3421 gaaggtaaca ggtcatttcc ccgttaccag gcatgggggc ctcgccatac cccgtaacga
     3481 ctgcgctcag tacccctgaa tccataacgg cattgtcatt tttcctgagc tgttccgccc
     3541 gtcgctccgc gcgacgacgt tctgtttcct cctgctcctt ccggcgcaat tcctcttcac
     3601 gtttacgcaa ctgctctctg agagctgcaa ggccggatgc gccccactgc tgccactgtg
     3661 tttcaaatgc cgtctgactc gcgggaatgt cctgtttctc caccttccgg taatgctgtc
     3721 gcaatttttc catttgctgg ctcagagact ggatttttcc ctgctcacta cggtattccg
     3781 gtgcagccca ttgcatcagg ggtatacggg aaaaaccgga cgaaaacggc gatgattttt
     3841 tcaggctggc agcatggctc atcgcttcct ttcgttcggt gttcagctct tcccagcgtc
     3901 tgcgcgtcgc ctgcatatct gccatcatcg acatggcgtt actggtgagt ttccatgccg
     3961 cgttactgtc cttcggcaac gcttcgatga tgtcacgtag tgtgtacgaa gttgcgttgt
     4021 caggaagcac gatattcaga aggtcagcca cacggcgggc aggctctgtc agcccgctat
     4081 tcctgagttc agtgcgggaa atctgtattt ccaggttaag cctgttaatc agacgattct
     4141 gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc acaggcagac tttccgaggt
     4201 gaataccagg cgttttctca agcccctgtc gctcgtatga acgatggtct atctcttcct
     4261 gataaccagc gttcgccagt gctctgtttg catggtcagc ccatgatgca cgccacgtct
     4321 cggcatgtgt ccggtcgttc cagtccctga cctttccgcc gaacccagca ggtccgacag
     4381 ccctcgtggt cagcatgatg tgcgcatggg gatttgtttt atccatgtga tggaacgcca
     4441 catcagcaat catgccctga ctgacaaaat tttctctgac aaaggcgaga acggtttccc
     4501 gtgcggcatc ctgctgcagt tcacggggga tagcgagttc aatttcccgc gcaagttgtg
     4561 agttcttccg gcgttcggcc ttctcaacgg cattccagag ttcggcacgg ttcgtacacc
     4621 aggcaggggc tgtcgcgggg gtcagaatca cagcttcagc cacaccgctt ttgcgggtgt
     4681 agtcgtgagt cagtccggta cgctcatcaa gcatcagtga accggaacga taggcagcgg
     4741 aagctacggc gctgtagccg ctgcttctgg agataatttt catgctgaga tgataaatcg
     4801 ccatttctgc cgtaatgcct ttttgtagtt ttctttcagc cccgcagggc gcacacaatc
     4861 gaaacggagt gaagattgta taagtgcgca cttcgtgatg tgccgtgcat tatgccagct
     4921 cagtttttaa actaccagtg acgcgctaaa atgcgatttt tcatcgcgca cggagttaca
     4981 tcatgagcag aacgctcgaa cagaagattg ctgacgctga ggcgagatta cagcgcctga
     5041 aggcgaagag ccggagcctg gacacagcgc agaaggttgt tgtaggtgca gcattgctgg
     5101 caaaggtcag aaagccggag gaagtgcagt tgcgagcctg gttactccag ttcctgaagg
     5161 cagaggtaac acggcaggct gatgtgagca ggatacagcc gttgattgac gaactgaacg
     5221 cgttgccaaa accagtcccc aaagaagtgt cagaaaacga ccaacaggcg tgaatgcact
     5281 gtccgctcgc cgcagcccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa
     5341 gtgaaccgta gccaggttga gcacatacgc acggtttttt gacaggtgat atgcgcttaa
     5401 cccgcatgaa tactggattt ccacagggtt gagcgggagt tttactaaag tccgcgaacc
     5461 tgttttttgt aacatgttgt ttttgctttt ttttcctttt acgcccccca agttttccac
     5521 aggctcgctg ttaagttttt agatcttgtt tttaagatct tgtttttaat acttgtttta
     5581 gggtcgaatt ttcttatgga attcaatagt ttagaagact ataggtaata taatttaagg
     5641 taaaggcaat atgatttaag gtggataggc aatatgattt aaggtaaagg caatatgatt
     5701 taaggtaata tccacaggaa aagggatatt aaaattaaat catattgcct ttacctaagg
     5761 gaatgtttat attttactca ttgccttagg ttgagggatt tacaatgtcg gatttgattg
     5821 cctacaaatc caatgcactg gttgaggcca gctataaatt gactcttcag gaacagcgat
     5881 tcttgttact ttgcattggt cgattgaagt cgggttctga tgcagaatcc actgaattgc
     5941 aaaagaccat gactgttacc gccgaagagt atttcgactc attccctgat atggggcgga
     6001 aaaatgccga ggttcagcta caggaagcaa ttgatcgttt gtgggacagg tcgatcatcc
     6061 ttaaggacga tgagaagcgc gaggagttcc gctggatcca gtatcgggca cagtacgcta
     6121 aaggtgaggg caaagcccaa atcacttttt cagatgcggt catgccttat ctgacacagt
     6181 tgaaggggca atttactaga gttgtaatta agaatatctc caatttatca agaagttaca
     6241 gcataagaat atacgaactt ctccagcaat tccgcagcac aggggaacgt attatcgccc
     6301 tcgatgattt tcgctcaatg cttgatatag agcataaata tcaaacatat aagtctctga
     6361 accagcaatt attaagacct tgcgttgacg aactgaatca aaaaagcgac ttggctgtaa
     6421 cgcttgagac gatcaagaag gggcgcacgg ttgtagccct gcattttcgc ttcaaggaag
     6481 acaaacagat caagatgacg atctaaaatc gattttaagg catctaaaac gccatctggt
     6541 tcgttttttt ggtgtcaggt atgcgatggt actaccaagc aaataaattg cgtcaaaatc
     6601 gattttagag agcggtatgg caaaccatgt cgaacattac ttgttttact tgacgtgaga
     6661 gatgcgttaa tgccgccagc agcagtcccc catcgaagtg actcatgcgt gactaaagag
     6721 caagcctgag caacgtagga atctccaacc ttcaggacgg gatggatatc aagcaccagg
     6781 gcacacttga aaacaaaacc tcgcctctgg caaagcgcgt cctttcacga tgacgtagag
     6841 gtatttgaat atctgtagga gttcatcacg ctattttaac gcttaaaaag cgcgtttctg
     6901 attgtgtaac taaacacagg tagtaattag taggtgtggg gggggcagaa acgcgcaatt
     6961 aatatttatc gtgaatttga ttttatgtct agtatcgaga aacagaatat aaaaagatta
     7021 tctttggaaa tatagaaaaa aaataaaata ataaactaaa aacaatcaaa aaataataaa
     7081 tattacactg caacatggca acaatatatt cattcatata gtgatataag ctatggtttt
     7141 caaaattttc cttgagctga agaacggagt tttatggcaa acgaggtaat cttgcacagg
     7201 aaaaataggt tgtatctatg tcatggtttt gttatttttt tttgctcagt tattgatggt
     7261 caatatgtaa aataaatcag gtacttcctt acacttaccc tcattcattg agggtttttt
     7321 ttatccatat ctaagttgac atcctccatg ccctgaagga cggggtttta cagcacaccg
     7381 gataactatc tcgacatgga tattgataca cgttaaaaat acgtgaggcg tttctggcag
     7441 atatctgagg tctatttcgc cgtgttagcc acactgtcag aaacgcgttc aatttctccc
     7501 gttgagctga tatgctcatc accgcaatca cggtagtatc cgaagacgaa ccgttaatgc
     7561 atcttccccc cgatactggg ctttcggggg gaagatttac tcggtgcgcc gtaaaacccc
     7621 gtccttcagg gcatggagga tgtcaacacg aatcccctat cttcttttca gtaagaaacg
     7681 gtcattgaaa acttatataa cactacattt ggtgtgtatt ttgcgataaa aatatcccct
     7741 atcctaatga tagggaatac aaacgatgaa ttaacagttc ttaattctca cgtgaaaaga
     7801 tagattttaa cgaagacaca atcagcagtc ttatatcgaa tggctatcga tcctgaatcc
     7861 tgatgagtgg ggatgtttct gtactaccaa tgaaaagcta tccggctaga gactttacga
     7921 tacagaaaca cccctctttc ttttatctca tcagatgcca tagcgcaaaa caatttcgtt
     7981 tcacagatgt gacttacaat caaactgtgc aacataaatc acaaaacaag aaggttttgt
     8041 tattattttg ttgatgagtt gtttttcgtt ggttgttttt ttgaggcgta catacgcgca
     8101 caaatgtaaa actaaattcg tgttttcgat aattttaagg ccacaatcat tatggcattt
     8161 tttatcctga tcgccgtcaa aatctgtcct tcatggcagg gtttacggcg atccggataa
     8221 caccaaagat acctacatgc tcattcccct gtggtttcca ggatatcttt cctgttttct
     8281 gtgctgttca tcgcgccgcc attcgtccca ggtacgatta aaacccagag agcggaacag
     8341 atgatccggc tgtttttctg gtgtattgac ggcagcaaaa agcggtgcct cacgggtttt
     8401 cagatgaaac ttcataagtt ccggtgcagg tgatatctgg ctgtctgcat cgtaattgac
     8461 ggcaacacca taccctgcat accgtaaccc cttgtcggtc agcgcactga tgaccagatg
     8521 cctgttatcg gctgagggaa cggcaaacgc gggatacagc gtaccattaa gccggacaac
     8581 accgcccaga gagtcatcca cgcccatgac cagcaaaagc tgaagctggc gggtttcccg
     8641 gtcgacagaa ctcatgaccg gcatcagcgt cggtggcaga cgtccctgca taaaatccac
     8701 cttccttgcc gctggtgatg gtatggctgg ctggcggtct tctccaagac agaaagactc
     8761 cggccatgtc ttcggctggc ggagattgcg ggtatattgc ggccatgtct ggcaaacctg
     8821 cgtcagaagt tgctcaaagg tcgcagagga ataaacacgt tgcagtggtg gatgttggac
     8881 gatttcgctt cggtcacaga tataaccact cctgtcacgc attaaatcaa cacactcaaa
     8941 gattctggtg cggtatttct ccagctcgtt cccccagagc gtatattctc cgttccggtt
     9001 gtgaagcaga aggtaacagg tcatttcccc gttaccaggc atgggggcct cgccataccc
     9061 cgtaacgact gcgctcagta cccctgaatc cataacggca ttgtcatttt tcctgagctg
     9121 ttccgcccgt cgctccgcgc gacgacgttc tgtttcctcc tgctccttcc ggcgcaattc
     9181 ctcttcacgt ttacgcaact gctctctgag agctgcaagg ccggatgcgc cccactgctg
     9241 ccactgtgtt tcaaatgccg tctgactcgc gggaatgtcc tgtttctcca ccttccggta
     9301 atgctgtcgc aatttttcca tttgctggct cagagactgg atttttccct gctcactacg
     9361 gtattccggt gcagcccatt gcatcagggg tatacgggaa aaaccggacg aaaacggcga
     9421 tgattttttc aggctggcag catggctcat cgcttccttt cgttcggtgt tcagctcttc
     9481 ccagcgtctg cgcgtcgcct gcatatctgc catcatcgac atggcgttac tggtgagttt
     9541 ccatgccgcg ttactgtcct tcggcaacgc ttcgatgatg tcacgtagtg tgtacgaagt
     9601 tgcgttgtca ggaagcacga tattcagaag gtcagccaca cggcgggcag gctctgtcag
     9661 cccgctattc ctgagttcag tgcgggaaat ctgtatttcc aggttaagcc tgttaatcag
     9721 acgattctgt tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac aggcagactt
     9781 tccgaggtga ataccaggcg ttttctcaag cccctgtcgc tcgtatgaac gatggtctat
     9841 ctcttcctga taaccagcgt tcgccagtgc tctgtttgca tggtcagccc atgatgcacg
     9901 ccacgtctcg gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga acccagcagg
     9961 tccgacagcc ctcgtggtca gcatgatgtg cgcatgggga tttgttttat ccatgtgatg
    10021 gaacgccaca tcagcaatca tgccctgact gacaaaattt tctctgacaa aggcgagaac
    10081 ggtttcccgt gcggcatcct gctgcagttc acgggggata gcgagttcaa tttcccgcgc
    10141 aagttgtgag ttcttccggc gttcggcctt ctcaacggca ttccagagtt cggcacggtt
    10201 cgtacaccag gcaggggctg tcgcgggggt cagaatcaca gcttcagcca caccgctttt
    10261 gcgggtgtag tcgtgagtca gtccggtacg ctcatcaagc atcagtgaac cggaacgata
    10321 ggcagcggaa gctacggcgc tgtagccgct gcttctggag ataattttca tgctgagatg
    10381 ataaatcgcc atttctgccg taatgccttt ttgtagtttt ctttcagccc cgcagggcgc
    10441 acacaatcga aacggagtga agattgtata agtgcgcact tcgtgatgtg ccgtgcatta
    10501 tgccagctca gtttttaaac taccagtgac gcgctaaaat gcgatttttc atcgcgcacg
    10561 gagttacatc atgagcagaa cgctcgaaca gaagattgct gacgctgagg cgagattaca
    10621 gcgcctgaag gcgaagagcc ggagcctgga cacagcgcag aaggttgttg taggtgcagc
    10681 attgctggca aaggtcagaa agccggagga agtgcagttg cgagcctggt tactccagtt
    10741 cctgaaggca gaggtaacac ggcaggctga tgtgagcagg atacagccgt tgattgacga
    10801 actgaacgcg ttgccaaaac cagtccccaa agaagtgtca gaaaacgacc aacaggcgtg
    10861 aatgcactgt ccgctcgccg cagccccagc gaccgagcgt tagcgagcga gtgggcgagg
    10921 aagcggaagt gaaccgtagc caggttgagc acatacgcac ggttttttga caggtgatat
    10981 gcgcttaacc cgcatgaata ctggatttcc acagggttga gcgggagttt tactaaagtc
    11041 cgcgaacctg ttttttgtaa catgttgttt ttgctttttt ttccttttac gccccccaag
    11101 ttttccacag gctcgctgtt aagtttttaa atcttgtttt taagatcttg tttttaatac
    11161 ttgttttagg gtcgaa
//
