LOCUS       NZ_CP127313            11304 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Escherichia coli strain C51 plasmid pC5107, complete sequence.
ACCESSION   NZ_CP127313
VERSION     NZ_CP127313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35637912
            Assembly: GCF_030285565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Complete Genome Sequences of Three Commensal and Two Avian
            Pathogenic Escherichia coli Strains Isolated from Farm Animals in
            Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Laboratory of Metagenome Analysis, Skolkovo
            Institute of Science and Technology, Bolshoy Boulevard 30, bld. 1,
            Moscow 121205, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2023
            Assembly Method                   :: Flye v. 2.9.1
            Assembly Name                     :: E_coli_C51
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 16:50:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,401
            CDSs (total)                      :: 5,279
            Genes (coding)                    :: 4,916
            CDSs (with protein)               :: 4,916
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 204 of 363
            Pseudo Genes (incomplete)         :: 170 of 363
            Pseudo Genes (internal stop)      :: 64 of 363
            Pseudo Genes (multiple problems)  :: 67 of 363
            Pseudo Genes (short protein)      :: 2 of 363
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C51"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pC5107"
                     /geo_loc_name="Russia: Perm krai"
                     /collection_date="2020-07"
                     /collected_by="Kuznetsova Marina, Mihailovskaya Valentina"
     gene            complement(join(11291..11304,1..517))
                     /locus_tag="QRM70_RS26940"
                     /old_locus_tag="QRM70_26935"
     CDS             complement(join(11291..11304,1..517))
                     /locus_tag="QRM70_RS26940"
                     /old_locus_tag="QRM70_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<555..986)
                     /locus_tag="QRM70_RS26945"
                     /old_locus_tag="QRM70_26940"
                     /pseudo=""
     CDS             complement(<555..986)
                     /locus_tag="QRM70_RS26945"
                     /old_locus_tag="QRM70_26940"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1195..1518)
                     /locus_tag="QRM70_RS26950"
                     /old_locus_tag="QRM70_26945"
     CDS             complement(1195..1518)
                     /locus_tag="QRM70_RS26950"
                     /old_locus_tag="QRM70_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1583..1774
                     /locus_tag="QRM70_RS26955"
                     /old_locus_tag="QRM70_26950"
     CDS             1583..1774
                     /locus_tag="QRM70_RS26955"
                     /old_locus_tag="QRM70_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2977..3843)
                     /locus_tag="QRM70_RS26960"
                     /old_locus_tag="QRM70_26955"
     CDS             complement(2977..3843)
                     /locus_tag="QRM70_RS26960"
                     /old_locus_tag="QRM70_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3845..4897)
                     /locus_tag="QRM70_RS26965"
                     /old_locus_tag="QRM70_26960"
     CDS             complement(3845..4897)
                     /locus_tag="QRM70_RS26965"
                     /old_locus_tag="QRM70_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139589617.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDCFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(5378..5632)
                     /locus_tag="QRM70_RS26970"
                     /old_locus_tag="QRM70_26965"
     CDS             complement(5378..5632)
                     /locus_tag="QRM70_RS26970"
                     /old_locus_tag="QRM70_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_115722738.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5639..6169)
                     /locus_tag="QRM70_RS26975"
                     /old_locus_tag="QRM70_26970"
     CDS             complement(5639..6169)
                     /locus_tag="QRM70_RS26975"
                     /old_locus_tag="QRM70_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6207..6638)
                     /locus_tag="QRM70_RS26980"
                     /old_locus_tag="QRM70_26975"
                     /pseudo=""
     CDS             complement(<6207..6638)
                     /locus_tag="QRM70_RS26980"
                     /old_locus_tag="QRM70_26975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6847..7170)
                     /locus_tag="QRM70_RS26985"
                     /old_locus_tag="QRM70_26980"
     CDS             complement(6847..7170)
                     /locus_tag="QRM70_RS26985"
                     /old_locus_tag="QRM70_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7235..7426
                     /locus_tag="QRM70_RS26990"
                     /old_locus_tag="QRM70_26985"
     CDS             7235..7426
                     /locus_tag="QRM70_RS26990"
                     /old_locus_tag="QRM70_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8629..9495)
                     /locus_tag="QRM70_RS26995"
                     /old_locus_tag="QRM70_26990"
     CDS             complement(8629..9495)
                     /locus_tag="QRM70_RS26995"
                     /old_locus_tag="QRM70_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(9497..10549)
                     /locus_tag="QRM70_RS27000"
                     /old_locus_tag="QRM70_26995"
     CDS             complement(9497..10549)
                     /locus_tag="QRM70_RS27000"
                     /old_locus_tag="QRM70_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(11030..11284)
                     /locus_tag="QRM70_RS27005"
                     /old_locus_tag="QRM70_27000"
     CDS             complement(11030..11284)
                     /locus_tag="QRM70_RS27005"
                     /old_locus_tag="QRM70_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_115722738.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1869..1950)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(7521..7602)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2407..2594)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(8059..8246)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(4..1205)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5275..6857)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.674"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtctcactgc gttcagtttg ccgttgtcct tcgtccagtt cgtttcggct ttcatcccct
       61 tcggcaacac cagaaaacgt ccgttgctgt cctcgtgata acgcacgccc catgtcttcg
      121 cgttcagctt ctccagactc tctttctgct cgcggagaat attcatattc gcgtttatct
      181 tcatgccctg gtaccacagc agactcccgg tcgtggcaaa catcagtact gagacgataa
      241 tcagccacag ccagcgtttc acgctcattt tcagtacggt tttccagtgc gcgtccatcg
      301 tatcgacggt cgttttctga tacctctcca gcagttcttt cagggctgtt gtatggtcgt
      361 tgatagcgtt cctgattaaa gattcgtttg aggtcagttc cctgtttaca gattcttcga
      421 gcttcctgaa ctcgtcgttc agcattttct ctgtagattc ggctctctct ttcagctttt
      481 tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa acgctccttt cagtctgata
      541 ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct gaccacttca
      601 agccccgcct ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc tccggcatgg
      661 taaagcgcgt ctatcccgcg tgtgacgccc tcagcaagcg cctgtttcgt ttccggcagg
      721 ttatcaggaa gggtcagcgt ccggcggttc tccggggcgt tggggtcatg cagaccgtaa
      781 tggtgattca ccagcgtctg ccaggcatca attctcggcc tgtctgcccg gtcgtagtac
      841 ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc
      901 cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttctc gagaccgggc
      961 atcagtacac gctcaaagct cgccatcact ttttcacgcc ctccaggcgg cagctccttc
     1021 tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt
     1081 tcccggacct cttccggatt cccctgaagc accgttgccc cttcccggtt acgctccctt
     1141 cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg aaacttaact
     1201 atcatcccgc gccccctgtt ccctgaccgc cagacgcagc cggcgcagct catccccgat
     1261 ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc cgctgttcac
     1321 cttcctcgct gtctggttaa ggttattccc gatggcggcc agctgacgca gtaacggcgg
     1381 tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga cccgccgcat
     1441 ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt
     1501 aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccca tgaacagaac
     1561 ccccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     1621 cttcatcaga ggccagacat taacgctgct ggagaagctc aacgaactgg acgcggatga
     1681 acaggcagac atctgtgaat cgcttcacga ccacgccgat gagctttacc gcagttgcct
     1741 cgcacgcttc ggggatgacg gtgaaaaccg ctgacacatg cagctcccgg agacggtcac
     1801 agcttgtctg tgagcggatg ccgggagcag acaagcccgc cagggcgcgt caggccgttg
     1861 acctttgggg tgtcggggcg aagccctgac ccagtcacgt agcgatagcg gagtgtatac
     1921 tggcttaacc attcggcatc agtgcggatt gtatgaaaag tgcgccatgt ccggtgtgaa
     1981 atgccgcaca gatgcgtaag gagaaaatgc cagttcaggc gctcttccgc ttcctcgctc
     2041 actgactcgc tacgctcggt cgttcggctg cggcgagcgg actctccttt ctcaaaaacg
     2101 ctaaccctgt tatccacaga acccggggat aaaccaggaa agaacatgtg cgcaaaaaac
     2161 gaaaccccgg gaaaggccgc gccggaggcg ctttttccat aggctccgcc cccctgacgg
     2221 acttcacaaa acagacactt tttcggtgca tctgtgggag ccttgcggct caaccatgaa
     2281 atccgccagc ggtggcgaaa cccgacgtgg cttaaagatc atcaccgttt ccggtgaatc
     2341 gctccctcct gcgcccccac gttcccgcca tatcgattac cggatacctt ttccgccttt
     2401 ctcccttacg ggacacgtgg cgctttctca tagctcacgt cttctggtat ctcggggcga
     2461 cttaggcagt tcgctccggg ctgggctgta tgcaggaacc ccccgttcag cccgactgct
     2521 gcgccttctc cggtaactgt tctcttgggt ccaacccgga aaagacacca caaagcgcca
     2581 ctggcagcag ccattggtaa aagtcatgtg cgagatattt agagagtact tgaagtggct
     2641 gccggagtgc ggctacacta gaagaacaat tttggtgact cgtctcgcat aagacagtta
     2701 cctggttaag cagtctgcaa gctgacttaa ccttcgatca aaccgccctg caaggcggtt
     2761 ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagag gatcctttga
     2821 tctttttgtt acaaacgtct ctatttgact cccctgagag cgttttagcg cgcctctgac
     2881 ccccttggtt acattccgac accagaaacg ctcccgtttt ttacgtgcat ttggcacggt
     2941 atcgatggag gccggggggc aatcctgaac agaatgttac tcctctgagt attttctgtt
     3001 aagaaaatat acacttagca cagttacaat aaatcctacc ccctgtaaga atgtgaattg
     3061 ttcatccaaa aacaaccatg acaaaaataa tgcaactagc ggaataaagt acaacattat
     3121 ccctgccaaa tgaactggga gccaacgcaa ggagttcaag taaaaacaaa aagctaaaaa
     3181 atattgtaat attccagatg aaaaaacaat aatcacggat tccaacggaa gataaattga
     3241 tgatttagat ataaaaataa agagcgatga cagtataagg gagaatgtca actgtccgct
     3301 caataatgtg ataggctcaa acgatttaat aaggcgagac gatataacaa cataaaatgc
     3361 tgccgataac acaccggcaa caacaagaca atatccaaca atattattcg atccattact
     3421 gatgtctggc aaggctacca aaatactgcc aacaattgca cctataagga acaatataaa
     3481 atattgcggg gaattaattt taactgagca aataagaaca gacaacacca atataaacac
     3541 tgattcaagt gaaaaaataa ttgatgcgct tcctgctgaa ataaaataaa gtccgtagag
     3601 tgtaaaagta tacgctaaga atggttcaaa aacgcctgtc gcataagcaa gtatcgcttt
     3661 tgagtttaat gggagttttt ttctttttat aaaaagaact atccaagata gtattactgc
     3721 actacaaatc tgccagaaaa acacccctcc ggcatctagt tcttcaactc cttttttgct
     3781 cattacgata gctgcccccc atgagaacgc tgctaaaaaa gcaaaaagca ttcctttaat
     3841 catattaatc atcctctttc tcataaaggg gtttgttttc aaatttcctt ggttgaaagg
     3901 agttaaaaaa catagatgca tcatctacag gaataccaat aaactctcca taataaggat
     3961 catctatagg aaactcttct aatgaaacat aaaacaatac attccagttg aagatcattg
     4021 gatcactatt ttctatttca tggagcaatc tttcatcctt agttaattct cctggaatta
     4081 atacatcagt atgataaaaa ccgctgtaat aagaactatt accggtgtgc actgggcatc
     4141 caaaacaatc caaattagat tctgaatgcc atgttgtaga aatcccttcc ggatgtgaat
     4201 acccaccctt accaacagac tgaatagtca tgttacgaat atccgctaac gcttgttctt
     4261 gatctttata gaaacctttt tctatcgcca gcctcataaa atgccccggg gcatttacag
     4321 aaataagtat tccccctggt attcttctat catgatttct tgttctatga gcagaagagt
     4381 aaaaaacatt aacaccagcc ttatatattt ttagcccatc tttatcctga aatggtacgc
     4441 tttcagtata aataacatcc tcttttattg gatagcactc tggcatatgc agcgaacaaa
     4501 tatatttttg tatgtccact aactcttctg atggacgagc aaaggcaagt tgtggatgaa
     4561 taaaataaat gagatgcgca ctgacctctc ctcttcttgc gcgttcttta aaacgaactc
     4621 tttcattacg caaaaaatca aacaaatgac catcatcatt atatagttgg gggctattta
     4681 caatagataa atggaaatca agccctttaa ttttcttgct tgccaattta ccaaatacac
     4741 agggctgatt gcctgacacc cattttctta gtaaatcttc aattttatct ttagaagttt
     4801 cgttaaagat aaatgaatga atattatcaa tatcttttcc aaatgaaaaa ctggatgata
     4861 attgtggtag tttatttata cgttgttgaa gattcatgaa agctcccaca agacacctca
     4921 tatatttttt tatatactga aactacatta tatttattac cggcgtaaaa cgaaataatt
     4981 tattatcgtt accgatattt taaaaaaatc attatttgca cagtgttcga gttgttagta
     5041 tcactccttc ctgaaaatcc gctccattta ccataacgtc gcattgtacg caccgccaaa
     5101 acgcgttcgg tgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc acccgaaaaa
     5161 ctgccgcgat cgcacctgat aaattttaac tgcatgaata cctatgcaac cagatgctac
     5221 aggccacatt agcccgcctt aatccactga acacgtcatt tttcatggtt tcaccatccc
     5281 agcgaagggc catccagtgt ccgctcctgg atttccggct gacgctcccg ctccagagat
     5341 aactcatgtt cgcgttcctg catcagccgt tcatccctta tctcctgttc tcgctgtata
     5401 actggctcaa gcgttctgtc cgctcgctca agcgttctgt ccgctcgctc aagtgctgca
     5461 cctgctgact caactgcatg actcgcccgt tcagcatcgc gttgtcccgt tgcgtaagcg
     5521 aaaacatttt ctgcaattcc acgaaggcgt tctcccattc gttcagccgc tgcatatagt
     5581 cctgttgcag ttgctctaat gcgttcagca aatgtctttc cagctccgtc atgctctgtc
     5641 actcctgcac cagtctcact gcgttcagtt tgccgttgtc cttcgtccag ttcgtttcgg
     5701 ctttcatccc cttcggcaac accagaaaac gtccgttgct gtcctcgtga taacgcacgc
     5761 cccatgtctt cgcgttcagc ttctccagac tctctttctg ctcgcggaga atattcatat
     5821 tcgcgtttat cttcatgccc tggtaccaca gcagactccc ggtcgtggca aacatcagta
     5881 ctgagacgat aatcagccac agccagcgtt tcacgctcat tttcagtacg gttttccagt
     5941 gcgcgtccat cgtatcgacg gtcgttttct gatacctctc cagcagttct ttcagggctg
     6001 ttgtatggtc gttgatagcg ttcctgatta aagattcgtt tgaggtcagt tccctgttta
     6061 cagattcttc gagcttcctg aactcgtcgt tcagcatttt ctctgtagat tcggctctct
     6121 ctttcagctt tttctcgaac tccgcgcccg tctgtaaaag attgctcata aaacgctcct
     6181 ttcagtctga tattcctccc gccgttcggg tctgcaatgc tgatactgct tcgcgtcacc
     6241 ctgaccactt caagccccgc ctctgtgagc gcctgaatca catcctgacg gccttttatc
     6301 tctccggcat ggtaaagcgc gtctatcccg cgtgtgacgc cctcagcaag cgcctgtttc
     6361 gtttccggca ggttatcagg aagggtcagc gtccggcggt tctccggggc gttggggtca
     6421 tgcagaccgt aatggtgatt caccagcgtc tgccaggcat caattctcgg cctgtctgcc
     6481 cggtcgtagt acggctggag gcgttttccg gtctgtagct ccatgttcgg aatgacaaaa
     6541 ttcagctcaa gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgatttttc
     6601 tcgagaccgg gcatcagtac acgctcaaag ctcgccatca ctttttcacg ccctccaggc
     6661 ggcagctcct tctccgcgaa cgacagaaca cctgacgtgt atttcttcgc aaatggcgtg
     6721 gcatcgatga gttcccggac ctcttccgga ttcccctgaa gcaccgttgc cccttcccgg
     6781 ttacgctccc ttcccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca
     6841 tgaaacttaa ctatcatccc gcgccccctg ttccctgacc gccagacgca gccggcgcag
     6901 ctcatccccg atggccatca gtgcggccac cacctgaacc cggtcaccgg aagaccactg
     6961 cccgctgttc accttcctcg ctgtctggtt aaggttattc ccgatggcgg ccagctgacg
     7021 cagtaacggc ggtgccagtg tcggcagctt cccggaacgg gcaaccggct cacccaggca
     7081 gacccgccgc atccataccg ccagttgttt accctcacag cgttccagta accgggcatg
     7141 ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc
     7201 catgaacaga accccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct
     7261 taacatggcc cgcttcatca gaggccagac attaacgctg ctggagaagc tcaacgaact
     7321 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgccg atgagcttta
     7381 ccgcagttgc ctcgcacgct tcggggatga cggtgaaaac cgctgacaca tgcagctccc
     7441 ggagacggtc acagcttgtc tgtgagcgga tgccgggagc agacaagccc gccagggcgc
     7501 gtcaggccgt tgacctttgg ggtgtcgggg cgaagccctg acccagtcac gtagcgatag
     7561 cggagtgtat actggcttaa ccattcggca tcagtgcgga ttgtatgaaa agtgcgccat
     7621 gtccggtgtg aaatgccgca cagatgcgta aggagaaaat gccagttcag gcgctcttcc
     7681 gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggactctcct
     7741 ttctcaaaaa cgctaaccct gttatccaca gaacccgggg ataaaccagg aaagaacatg
     7801 tgcgcaaaaa acgaaacccc gggaaaggcc gcgccggagg cgctttttcc ataggctccg
     7861 cccccctgac ggacttcaca aaacagacac tttttcggtg catctgtggg agccttgcgg
     7921 ctcaaccatg aaatccgcca gcggtggcga aacccgacgt ggcttaaaga tcatcaccgt
     7981 ttccggtgaa tcgctccctc ctgcgccccc acgttcccgc catatcgatt accggatacc
     8041 ttttccgcct ttctccctta cgggacacgt ggcgctttct catagctcac gtcttctggt
     8101 atctcggggc gacttaggca gttcgctccg ggctgggctg tatgcaggaa ccccccgttc
     8161 agcccgactg ctgcgccttc tccggtaact gttctcttgg gtccaacccg gaaaagacac
     8221 cacaaagcgc cactggcagc agccattggt aaaagtcatg tgcgagatat ttagagagta
     8281 cttgaagtgg ctgccggagt gcggctacac tagaagaaca attttggtga ctcgtctcgc
     8341 ataagacagt tacctggtta agcagtctgc aagctgactt aaccttcgat caaaccgccc
     8401 tgcaaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
     8461 aggatccttt gatctttttg ttacaaacgt ctctatttga ctcccctgag agcgttttag
     8521 cgcgcctctg acccccttgg ttacattccg acaccagaaa cgctcccgtt ttttacgtgc
     8581 atttggcacg gtatcgatgg aggccggggg gcaatcctga acagaatgtt actcctctga
     8641 gtattttctg ttaagaaaat atacacttag cacagttaca ataaatccta ccccctgtaa
     8701 gaatgtgaat tgttcatcca aaaacaacca tgacaaaaat aatgcaacta gcggaataaa
     8761 gtacaacatt atccctgcca aatgaactgg gagccaacgc aaggagttca agtaaaaaca
     8821 aaaagctaaa aaatattgta atattccaga tgaaaaaaca ataatcacgg attccaacgg
     8881 aagataaatt gatgatttag atataaaaat aaagagcgat gacagtataa gggagaatgt
     8941 caactgtccg ctcaataatg tgataggctc aaacgattta ataaggcgag acgatataac
     9001 aacataaaat gctgccgata acacaccggc aacaacaaga caatatccaa caatattatt
     9061 cgatccatta ctgatgtctg gcaaggctac caaaatactg ccaacaattg cacctataag
     9121 gaacaatata aaatattgcg gggaattaat tttaactgag caaataagaa cagacaacac
     9181 caatataaac actgattcaa gtgaaaaaat aattgatgcg cttcctgctg aaataaaata
     9241 aagtccgtag agtgtaaaag tatacgctaa gaatggttca aaaacgcctg tcgcataagc
     9301 aagtatcgct tttgagttta atgggagttt ttttcttttt ataaaaagaa ctatccaaga
     9361 tagtattact gcactacaaa tctgccagaa aaacacccct ccggcatcta gttcttcaac
     9421 tccttttttg ctcattacga tagctgcccc ccatgagaac gctgctaaaa aagcaaaaag
     9481 cattccttta atcatattaa tcatcctctt tctcataaag gggtttgttt tcaaatttcc
     9541 ttggttgaaa ggagttaaaa aacatagatg catcatctac aggaatacca ataaactctc
     9601 cataataagg atcatctata ggaaactctt ctaatgaaac ataaaacaat acattccagt
     9661 tgaagatcat tggatcacta ttttctattt catggagcaa tctttcatcc ttagttaatt
     9721 ctcctggaat taatacatca gtatgataaa aaccgctgta ataagaacta ttaccggtgt
     9781 gcactgggca tccaaaacga tccaaattag attctgaatg ccatgttgta gaaatccctt
     9841 ccggatgtga atacccaccc ttaccaacag actgaatagt catgttacga atatccgcta
     9901 acgcttgttc ttgatcttta tagaaacctt tttctatcgc cagcctcata aaatgccccg
     9961 gggcatttac agaaataagt attccccctg gtattcttct atcatgattt cttgttctat
    10021 gagcagaaga gtaaaaaaca ttaacaccag ccttatatat ttttagccca tctttatcct
    10081 gaaatggtac gctttcagta taaataacat cctcttttat tggatagcac tctggcatat
    10141 gcagcgaaca aatatatttt tgtatgtcca ctaactcttc tgatggacga gcaaaggcaa
    10201 gttgtggatg aataaaataa atgagatgcg cactgacctc tcctcttctt gcgcgttctt
    10261 taaaacgaac tctttcatta cgcaaaaaat caaacaaatg accatcatca ttatatagtt
    10321 gggggctatt tacaatagat aaatggaaat caagcccttt aattttcttg cttgccaatt
    10381 taccaaatac acagggctga ttgcctgaca cccattttct tagtaaatct tcaattttat
    10441 ctttagaagt ttcgttaaag ataaatgaat gaatattatc aatatctttt ccaaatgaaa
    10501 aactggatga taattgtggt agtttattta tacgttgttg aagattcatg aaagctccca
    10561 caagacacct catatatttt tttatatact gaaactacat tatatttatt accggcgtaa
    10621 aacgaaataa tttattatcg ttaccgatat tttaaaaaaa tcattatttg cacagtgttc
    10681 gagttgttag tatcactcct tcctgaaaat ccgctccatt taccataacg tcgcattgta
    10741 cgcaccgcca aaacgcgttc ggtgcgatca cggcagcaga caggtaaaaa tggcaacaaa
    10801 ccacccgaaa aactgccgcg atcgcacctg ataaatttta actgcatgaa tacctatgca
    10861 accagatgct acaggccaca ttagcccgcc ttaatccact gaacacgtca tttttcatgg
    10921 tttcaccatc ccagcgaagg gccatccagt gtccgctcct ggatttccgg ctgacgctcc
    10981 cgctccagag ataactcatg ttcgcgttcc tgcatcagcc gttcatccct tatctcctgt
    11041 tctcgctgta taactggctc aagcgttctg tccgctcgct caagcgttct gtccgctcgc
    11101 tcaagtgctg cacctgctga ctcaactgca tgactcgccc gttcagcatc gcgttgtccc
    11161 gttgcgtaag cgaaaacatt ttctgcaatt ccacgaaggc gttctcccat tcgttcagcc
    11221 gctgcatata gtcctgttgc agttgctcta atgcgttcag caaatgtctt tccagctccg
    11281 tcatgctctg tcactcctgc acca
//
