LOCUS       NZ_CP127309            10798 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Escherichia coli strain C51 plasmid pC5108, complete sequence.
ACCESSION   NZ_CP127309
VERSION     NZ_CP127309.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35637912
            Assembly: GCF_030285565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Complete Genome Sequences of Three Commensal and Two Avian
            Pathogenic Escherichia coli Strains Isolated from Farm Animals in
            Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Laboratory of Metagenome Analysis, Skolkovo
            Institute of Science and Technology, Bolshoy Boulevard 30, bld. 1,
            Moscow 121205, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2023
            Assembly Method                   :: Flye v. 2.9.1
            Assembly Name                     :: E_coli_C51
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 16:50:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,401
            CDSs (total)                      :: 5,279
            Genes (coding)                    :: 4,916
            CDSs (with protein)               :: 4,916
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 204 of 363
            Pseudo Genes (incomplete)         :: 170 of 363
            Pseudo Genes (internal stop)      :: 64 of 363
            Pseudo Genes (multiple problems)  :: 67 of 363
            Pseudo Genes (short protein)      :: 2 of 363
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127309.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C51"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pC5108"
                     /geo_loc_name="Russia: Perm krai"
                     /collection_date="2020-07"
                     /collected_by="Kuznetsova Marina, Mihailovskaya Valentina"
     gene            175..1101
                     /locus_tag="QRM70_RS24960"
                     /old_locus_tag="QRM70_24955"
     CDS             175..1101
                     /locus_tag="QRM70_RS24960"
                     /old_locus_tag="QRM70_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_285965586.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLCELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQYELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1104..1637
                     /locus_tag="QRM70_RS24965"
                     /old_locus_tag="QRM70_24960"
     CDS             1104..1637
                     /locus_tag="QRM70_RS24965"
                     /old_locus_tag="QRM70_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_285965587.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEIRSEVQKLDVAGLIGERISQDFQRLEESVAKMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            1857..2981
                     /locus_tag="QRM70_RS24970"
                     /old_locus_tag="QRM70_24965"
     CDS             1857..2981
                     /locus_tag="QRM70_RS24970"
                     /old_locus_tag="QRM70_24965"
                     /inference="COORDINATES: protein motif:HMM:NF013211.2"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_285965590.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WRPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPASGNKLTRDTIKSFIEFKAKLEDDAVKDAISKVNDFYKELASKYGEKLAKEA
                     RALADEAKGKTLRNSKEAIKAFDKYKNVLNKKFSVSDREAIAKSLESLNKEQMAKQLKI
                     FGKAFGVVGQVTQWGDFINNLAKGFKTGDWNDAIISGEKIAAGKVASVMVTVAFSAMAV
                     NPVGILGFAVIMAVTSSLITDERLKQLNDFIKTL"
                     /name="colicin-like pore-forming protein"
     gene            3892..4806
                     /locus_tag="QRM70_RS24975"
                     /old_locus_tag="QRM70_24970"
     CDS             3892..4806
                     /locus_tag="QRM70_RS24975"
                     /old_locus_tag="QRM70_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_285965589.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLACGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWTDRNAPAPNVTVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPDLSQWRIAVIQRVEMYNAQLPVPLSP
                     AECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(4999..5397)
                     /locus_tag="QRM70_RS24980"
                     /old_locus_tag="QRM70_24975"
     CDS             complement(4999..5397)
                     /locus_tag="QRM70_RS24980"
                     /old_locus_tag="QRM70_24975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            5574..6500
                     /locus_tag="QRM70_RS24985"
                     /old_locus_tag="QRM70_24980"
     CDS             5574..6500
                     /locus_tag="QRM70_RS24985"
                     /old_locus_tag="QRM70_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_285965586.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLCELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQYELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            6503..7036
                     /locus_tag="QRM70_RS24990"
                     /old_locus_tag="QRM70_24985"
     CDS             6503..7036
                     /locus_tag="QRM70_RS24990"
                     /old_locus_tag="QRM70_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_285965587.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEIRSEVQKLDVAGLIGERISQDFQRLEESVAKMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            7256..8380
                     /locus_tag="QRM70_RS24995"
                     /old_locus_tag="QRM70_24990"
     CDS             7256..8380
                     /locus_tag="QRM70_RS24995"
                     /old_locus_tag="QRM70_24990"
                     /inference="COORDINATES: protein motif:HMM:NF013211.2"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_285965588.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPADTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WRPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPASGNKLTRDTIKSFIEFKAKLEDDAVKDAISKVNDFYKELASKYGEKLAKEA
                     RALADEAKGKTLRNSKEAIKAFDKYKNVLNKKFSVSDREAIAKSLESLNKEQMAKQLKI
                     FGKAFGVVGQVTQWGDFINNLAKGFKTGDWNDAIISGEKIAAGKVASVMVTVAFSAMAV
                     NPVGILGFAVIMAVTSSLITDERLKQLNDFIKTL"
                     /name="colicin-like pore-forming protein"
     gene            9291..10205
                     /locus_tag="QRM70_RS25000"
                     /old_locus_tag="QRM70_24995"
     CDS             9291..10205
                     /locus_tag="QRM70_RS25000"
                     /old_locus_tag="QRM70_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_285965589.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLACGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWTDRNAPAPNVTVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPDLSQWRIAVIQRVEMYNAQLPVPLSP
                     AECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(10398..10796)
                     /locus_tag="QRM70_RS25005"
                     /old_locus_tag="QRM70_25000"
     CDS             complement(10398..10796)
                     /locus_tag="QRM70_RS25005"
                     /old_locus_tag="QRM70_25000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          3893..4806
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.742"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9292..10205
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.742"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          176..1098
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5575..6497
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgtgtgctc cctggtgttt ttttgcgatg tgttgtggtc gtgcagtata tcgcttgcgg
       61 tcggtgggtg caggggggta tcttccgtgc taattcgtaa aattagaagg aagcgcactt
      121 atatgtttct ctccttcgtc gagaaacagt gcgctcctgc ggagggctga aaccgtggca
      181 acctatcacc tcagtgttaa atttggtggc aaggggcagg ctgcaaacca cgccgattac
      241 atcgagcgca aagagaaata ccgcgaccgt caggatttgg aatattccgc gcacggaaac
      301 atgccggaat gggctaggga taatccatcc catttctggc aggcggctga ccagtttgaa
      361 cgtgcgaacg gttcgactta tcgggaactg gaaatcgcgc tgccccgtga actgacaccg
      421 gaacagcgcc ttgaactggt gcaggatttt gtacgtcagg aagccggcga acggcacgcc
      481 tggagttttg ccattcacaa tccgaaagcg agcattgacg gcggcgaaca gcctcatgcg
      541 catatcatga tgagtcagcg tgtgaatgat ggcattgaac gtacaccgga acagtatttc
      601 cggcgctata acgccaggta tcccgaccgg ggaggggcga aaaaggattc cggaaacctg
      661 acccccactc agcaaaaaga gcagttgtgt gaattgcgaa aacgctggga ggtgaagcac
      721 aacgaacata tgagaaaaca tggctttgaa aggggcttta ttgactgccg gacgctgaaa
      781 gaacagggca ttgaacgacg tcccgaagtg catctgggtt ttgaagctgc cggacggctt
      841 gatgatgccc agcgtcatga catcatgcaa aaacggtctg aaccggacta ttaccgccat
      901 cttgaacagg cgcgaatggt cgggcatgac atcagggaat ggctggatac accggttaag
      961 caatatgaac tggtcagaag gctgcaacat gatgttgcgc gggagcatgg ctttgacatg
     1021 agagacagag tgagcagaca gaaccgaact gaacctgaac gggttaaatc accgtcacgt
     1081 ggattttcta tgggattctg aacatggcca gcacagaaga aaaattactc acgctcctgt
     1141 tatcggtgga gcatctgcaa cataccgcaa tggagcaaca gaaagctgtt gctgattctc
     1201 tggctgtggc aagggaaaca cttcgcggat atcgggatat ttctgacggt gtggcaaaac
     1261 aggtcaggga tgaaatcaga agcgaggtgc agaagctgga tgttgcaggg ctgataggtg
     1321 agcgcatcag ccaggatttt cagcggctgg aagagtcagt cgcaaaaatg aaggcaaacg
     1381 cggatgccct gaacagggat attgcgacaa ttcacagcaa tatcgtgcag gaatacaaca
     1441 cccttaaagg gtatagctgg aaatggctgg gtggggtgtc agtggctttt ctggttatgt
     1501 tctgtgtgct cggatgggga atgaaaacaa tgctcgacag gaactacaac gccacagcgg
     1561 cggtgtatca gaaagttgtg acactggaag ataaactggt ggagaaacct gtcacgggta
     1621 agagccggaa aaactgacgc gcttcgcttg tccaacgcct cacgacccgc gaaaggattt
     1681 cgcgttccgt tcaccgtttt catgagtgat tcctccgaac tgccaaaaaa tatcccccct
     1741 gacttgacag aaaaaataat ggcgagtact ttttgatctg tacataaaac cagtggtttt
     1801 atgtacagca ttaactatgt aattaattat tttaacgctt aaaagaggga atttttatga
     1861 gtggtggaga tggtcgtggc cctggtaata cagggcttgg acataacggt ggtaaaccca
     1921 gtggaaacgt gaatggctca tctggacatg gaggaccatc atctggtggt gggtatcatt
     1981 acaatccggg aaaaggtcct gcaagcactg taggtcctga cggggaggtg catatcaaca
     2041 ttaccggtgg tatggaaaaa gatcctccag gaaaacatca cggtaatggt ggctcaggta
     2101 atggtcacgg tgctggaaat cataacagca attcaggcgg caatggaaaa acaacgtcta
     2161 ttcctggatg gagacctgac tctgggaaac ctggcagata tttaccgaca aataaagata
     2221 agttaattaa gggacttatt cagtcaatag atgaccattc tggaacgtac actatccatt
     2281 atactggtgg tgcagtagct acagtaactg taccaaacgg tgatgtgaat aaaatgaatg
     2341 ttcactataa tatcggtagt gggccggcat caggtaataa actgacccgt gacaccatta
     2401 aaagctttat agaattcaaa gccaaacttg aagatgatgc ggtaaaggat gcaatcagta
     2461 aagttaatga tttttacaag gaactggcat caaaatacgg agaaaagctg gcaaaagaag
     2521 ctagggctct tgccgatgag gctaaaggaa aaacgcttcg gaattctaaa gaggcaatta
     2581 aagcctttga caagtacaag aatgtactga ataagaaatt cagtgtatcc gaccgggaag
     2641 ccattgcaaa atctctggag tctctcaata aagagcagat ggctaaacag ttgaaaatct
     2701 ttggtaaggc ttttggtgtg gtgggtcaag taactcaatg gggtgatttc atcaacaatt
     2761 tagctaaagg attcaaaacc ggtgactgga atgacgctat tattagcggc gaaaagatag
     2821 ctgcgggtaa ggtggcatca gttatggtga cggtggcatt cagtgccatg gcagtaaacc
     2881 ctgtaggtat tttagggttt gcagtaatta tggctgtgac cagttcctta attacagacg
     2941 aaagactgaa gcaactaaat gattttataa aaacactata acaaagcaaa aaagcatgtc
     3001 attttaaatg acatgcttta catcctagat atttttttgt tttttataaa aaaataagtt
     3061 ataaatataa atccaattgg tggtaatgat agcaataagc atatctccgc tccccagtta
     3121 acaaaattaa atgtagttgt taaaaatttt acctttcgaa caaaaaacca aaagacaaaa
     3181 gggtatatta atgccccaga acaaaaaaac aaagaaacaa caaaaaacca aaaactattt
     3241 atttcatttt tgaaccaaat aaaaccgatc aacgctatat aaaaaaaagc ggagtaaaga
     3301 agatttttaa aataaaaaga taaagtcatt ttatccctcc atgagcatgt ttacgattct
     3361 gtgtaaatat attttctcag tagtgctcct catcgaactc gataataaag cggctcattg
     3421 ccattcgcca gtcttgttgt ggtattggga gccgcttgga attcggaaag aaaataaacc
     3481 acgttaagac tattatcccc gtgcagaacc attccatcca atgggagaaa acttgtcatt
     3541 ttgacctgtt cgcccttcgg aacagtcgaa accgatcgcg catcgctttc gtgcatagtt
     3601 atgcaacgcg ctaaaaacga ttctgacgca ttttttatgt ttaccctggt gtttccttgt
     3661 ctttttgcgt tttttgcgtc agaaagcgat tgagggcgtt ttaaggggtg cgtacaacgg
     3721 gagttatggt aaatggatcg gtttttcggg aaggatcgac aggatttgcc gttgggtgta
     3781 gtgtaagcga ctgaaaaaca aacgcccctg aaattcgcgt gagatccggc cagatttatc
     3841 acgaataaac aggggcgagg ttggtcgata acctggaagg aatcgtaaca catgagcgcc
     3901 gcgcttcaat acttcgaaga aaatttacct cacagaccgt atcacacgga cgatcttgcc
     3961 tgtggccttc gcatttccgg caaagggcgc gccctgcttg cgcgctacat tcagcagaac
     4021 cagccgcatg cgcaattctg gctggtcttt gacgttgacc gcgtgggggc ggcgatcgac
     4081 tggaccgacc ggaacgcccc tgcaccgaat gttaccgtta aaaatcctgt gaacggtcat
     4141 gctcacctgc tctacgcgct caatatcgcc gtgagaaccg cgccggacag ctcggttaag
     4201 gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg agaaactttg tgctgatgtg
     4261 aactacagcg gactgatttg taaaaatccg ttccacctgg aatggcaggt gatggagtgg
     4321 cgagaggaag cctataccct cgatgaactg gctgattatc tcgatttgag cgcctcagcg
     4381 cgccgtagca tcgataaaca ttacgggatg gggcgaaact gccacctgtt cgaaatgacg
     4441 cgcaaatggg cttacagggc gattcgtcag ggctggcccg atctttcgca gtggcggatc
     4501 gcagtgatcc agcgtgtcga aatgtacaat gcgcagcttc cggttccgct ttcaccggct
     4561 gaatgtcggg cgattggcag gagcatcgcg aaatacacgc acaggaactt tacaccggaa
     4621 acgttcgcac agtatgtggc tgatacgcac acaccagaaa ttcaggctgc acgcggtcgc
     4681 aagggcggtt ctaagtctaa gcgcagcaca gtggctacat ctgcacgcac gctgaaaccg
     4741 tgggaggcat tagggatcag tcgcgcctgg tattaccaac tgaaaaaacg aggtctcgta
     4801 gagtagacca aataagccta tatcagataa cagcgccttt ttggcgtctc tttgagcagc
     4861 cgtttttccc cgttcagccc tgcaatggtg ctgttttccg catgattgcg ctggtagtgc
     4921 tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc gagtaagcac cagggaatcg
     4981 gaacacggat ggaggcactt acgcgtctgg gtttggggat gtctccagat cgtacttaag
     5041 ttccttaagt gcctgtaata tcctgtcttt tttatagcat agctcgcctg tttttgtgtc
     5101 taccagtcct gcttcgatca gtagcccagc cgcgagaaag agccggtgat tgcgttctcg
     5161 tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt tttcgttcgc tctcctgtgc
     5221 ctgtaagtcg tttttttccg ctgttgccag tacgtggaga agtttgcgtt cctgttctgt
     5281 gagtcctgct tcgttgtcga gcaggtcaat aatctgtctc tgtcgtgcgg tttgttctcc
     5341 gagcttcctg atgaatccga tccgtttctt cagccattcg atgttgctga tgcgcatgtt
     5401 cgtgtgctcc ctggtgtttt tttgcgatgt gttgtggtcg tgcagtatat cgcttgcggt
     5461 cggtgggtgc aggggggtat cttccgtgct aattcgtaaa attagaagga agcgcactta
     5521 tatgtttctc tccttcgtcg agaaacagtg cgctcctgcg gagggctgaa accgtggcaa
     5581 cctatcacct cagtgttaaa tttggtggca aggggcaggc tgcaaaccac gccgattaca
     5641 tcgagcgcaa agagaaatac cgcgaccgtc aggatttgga atattccgcg cacggaaaca
     5701 tgccggaatg ggctagggat aatccatccc atttctggca ggcggctgac cagtttgaac
     5761 gtgcgaacgg ttcgacttat cgggaactgg aaatcgcgct gccccgtgaa ctgacaccgg
     5821 aacagcgcct tgaactggtg caggattttg tacgtcagga agccggcgaa cggcacgcct
     5881 ggagttttgc cattcacaat ccgaaagcga gcattgacgg cggcgaacag cctcatgcgc
     5941 atatcatgat gagtcagcgt gtgaatgatg gcattgaacg tacaccggaa cagtatttcc
     6001 ggcgctataa cgccaggtat cccgaccggg gaggggcgaa aaaggattcc ggaaacctga
     6061 cccccactca gcaaaaagag cagttgtgtg aattgcgaaa acgctgggag gtgaagcaca
     6121 acgaacatat gagaaaacat ggctttgaaa ggggctttat tgactgccgg acgctgaaag
     6181 aacagggcat tgaacgacgt cccgaagtgc atctgggttt tgaagctgcc ggacggcttg
     6241 atgatgccca gcgtcatgac atcatgcaaa aacggtctga accggactat taccgccatc
     6301 ttgaacaggc gcgaatggtc gggcatgaca tcagggaatg gctggataca ccggttaagc
     6361 aatatgaact ggtcagaagg ctgcaacatg atgttgcgcg ggagcatggc tttgacatga
     6421 gagacagagt gagcagacag aaccgaactg aacctgaacg ggttaaatca ccgtcacgtg
     6481 gattttctat gggattctga acatggccag cacagaagaa aaattactca cgctcctgtt
     6541 atcggtggag catctgcaac ataccgcaat ggagcaacag aaagctgttg ctgattctct
     6601 ggctgtggca agggaaacac ttcgcggata tcgggatatt tctgacggtg tggcaaaaca
     6661 ggtcagggat gaaatcagaa gcgaggtgca gaagctggat gttgcagggc tgataggtga
     6721 gcgcatcagc caggattttc agcggctgga agagtcagtc gcaaaaatga aggcaaacgc
     6781 ggatgccctg aacagggata ttgcgacaat tcacagcaat atcgtgcagg aatacaacac
     6841 ccttaaaggg tatagctgga aatggctggg tggggtgtca gtggcttttc tggttatgtt
     6901 ctgtgtgctc ggatggggaa tgaaaacaat gctcgacagg aactacaacg ccacagcggc
     6961 ggtgtatcag aaagttgtga cactggaaga taaactggtg gagaaacctg tcacgggtaa
     7021 gagccggaaa aactgacgcg cttcgcttgt ccaacgcctc acgacccgcg aaaggatttc
     7081 gcgttccgtt caccgttttc atgagtgatt cctccgaact gccaaaaaat atcccccctg
     7141 acttgacaga aaaaataatg gcgagtactt tttgatctgt acataaaacc agtggtttta
     7201 tgtacagcat taactatgta attaattatt ttaacgctta aaagagggaa tttttatgag
     7261 tggtggagat ggtcgtggcc ctggtaatac agggcttgga cataacggtg gtaaacccag
     7321 tggaaacgtg aatggctcat ctggacatgg aggaccatca tctggtggtg ggtatcatta
     7381 caatccggga aaaggtcctg cagacactgt aggtcctgac ggggaggtgc atatcaacat
     7441 taccggtggt atggaaaaag atcctccagg aaaacatcac ggtaatggtg gctcaggtaa
     7501 tggtcacggt gctggaaatc ataacagcaa ttcaggcggc aatggaaaaa caacgtctat
     7561 tcctggatgg agacctgact ctgggaaacc tggcagatat ttaccgacaa ataaagataa
     7621 gttaattaag ggacttattc agtcaataga tgaccattct ggaacgtaca ctatccatta
     7681 tactggtggt gcagtagcta cagtaactgt accaaacggt gatgtgaata aaatgaatgt
     7741 tcactataat atcggtagtg ggccggcatc aggtaataaa ctgacccgtg acaccattaa
     7801 aagctttata gaattcaaag ccaaacttga agatgatgcg gtaaaggatg caatcagtaa
     7861 agttaatgat ttttacaagg aactggcatc aaaatacgga gaaaagctgg caaaagaagc
     7921 tagggctctt gccgatgagg ctaaaggaaa aacgcttcgg aattctaaag aggcaattaa
     7981 agcctttgac aagtacaaga atgtactgaa taagaaattc agtgtatccg accgggaagc
     8041 cattgcaaaa tctctggagt ctctcaataa agagcagatg gctaaacagt tgaaaatctt
     8101 tggtaaggct tttggtgtgg tgggtcaagt aactcaatgg ggtgatttca tcaacaattt
     8161 agctaaagga ttcaaaaccg gtgactggaa tgacgctatt attagcggcg aaaagatagc
     8221 tgcgggtaag gtggcatcag ttatggtgac ggtggcattc agtgccatgg cagtaaaccc
     8281 tgtaggtatt ttagggtttg cagtaattat ggctgtgacc agttccttaa ttacagacga
     8341 aagactgaag caactaaatg attttataaa aacactataa caaagcaaaa aagcatgtca
     8401 ttttaaatga catgctttac atcctagata tttttttgtt ttttataaaa aaataagtta
     8461 taaatataaa tccaattggt ggtaatgata gcaataagca tatctccgct ccccagttaa
     8521 caaaattaaa tgtagttgtt aaaaatttta cctttcgaac aaaaaaccaa aagacaaaag
     8581 ggtatattaa tgccccagaa caaaaaaaca aagaaacaac aaaaaaccaa aaactattta
     8641 tttcattttt gaaccaaata aaaccaatca acgctatata aaaaaaagcg gagtaaagaa
     8701 gatttttaaa ataaaaagat aaagtcattt tatccctcca tgagcatgtt tacgattctg
     8761 tgtaaatata ttttctcagt agtgctcctc atcgaactcg ataataaagc ggctcattgc
     8821 cattcgccag tcttgttgtg gtattgggag ccgcttggaa ttcggaaaga aaataaacca
     8881 cgttaagact attatccccg tgcagaacca ttccatccaa tgggagaaaa cttgtcattt
     8941 tgacctgttc gcccttcgga acagtcgaaa ccgatcgcgc atcgctttcg tgcatagtta
     9001 tgcaacgcgc taaaaacgat tctgacgcat tttttatgtt taccctggtg tttccttgtc
     9061 tttttgcgtt ttttgcgtca gaaagcgatt gagggcgttt taaggggtgc gtacaacggg
     9121 agttatggta aatggatcgg tttttcggga aggatcgaca ggatttgccg ttgggtgtag
     9181 tgtaagcgac tgaaaaacaa acgcccctga aattcgcgtg agatccggcc agatttatca
     9241 cgaataaaca ggggcgaggt tggtcgataa cctggaagga atcgtaacac atgagcgccg
     9301 cgcttcaata cttcgaagaa aatttacctc acagaccgta tcacacggac gatcttgcct
     9361 gtggccttcg catttccggc aaagggcgcg ccctgcttgc gcgctacatt cagcagaacc
     9421 agccgcatgc gcaattctgg ctggtctttg acgttgaccg cgtgggggcg gcgatcgact
     9481 ggaccgaccg gaacgcccct gcaccgaatg ttaccgttaa aaatcctgtg aacggtcatg
     9541 ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc gccggacagc tcggttaagg
     9601 cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt gctgatgtga
     9661 actacagcgg actgatttgt aaaaatccgt tccacctgga atggcaggtg atggagtggc
     9721 gagaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc gcctcagcgc
     9781 gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc gaaatgacgc
     9841 gcaaatgggc ttacagggcg attcgtcagg gctggcccga tctttcgcag tggcggatcg
     9901 cagtgatcca gcgtgtcgaa atgtacaatg cgcagcttcc ggttccgctt tcaccggctg
     9961 aatgtcgggc gattggcagg agcatcgcga aatacacgca caggaacttt acaccggaaa
    10021 cgttcgcaca gtatgtggct gatacgcaca caccagaaat tcaggctgca cgcggtcgca
    10081 agggcggttc taagtctaag cgcagcacag tggctacatc tgcacgcacg ctgaaaccgt
    10141 gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga ggtctcgtag
    10201 agtagaccaa ataagcctat atcagataac agcgcctttt tggcgtctct ttgagcagcc
    10261 gtttttcccc gttcagccct gcaatggtgc tgttttccgc atgattgcgc tggtagtgct
    10321 cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg agtaagcacc agggaatcgg
    10381 aacacggatg gaggcactta cgcgtctggg tttggggatg tctccagatc gtacttaagt
    10441 tccttaagtg cctgtaatat cctgtctttt ttatagcata gctcgcctgt ttttgtgtct
    10501 accagtcctg cttcaatcag tagcccagcc gcgagaaaga gccggtgatt gcgttctcgt
    10561 cgttgttttt ttccctcgat tcgtttttgg accgcctgtt ttcgttcgct ctcctgtgcc
    10621 tgtaagtcgt ttttttccgc tgttgccagt acgtggagaa gtttgcgttc ctgttctgtg
    10681 agtcctgctt cgttgtcgag caggtcaata atctgtctct gtcgtgcggt ttgttctccg
    10741 agcttcctga tgaatccgat ccgtttcttc agccattcga tgttgctgat gcgcatgt
//
