LOCUS       NZ_CP127259            17105 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Escherichia coli strain Q5 plasmid pQ504, complete sequence.
ACCESSION   NZ_CP127259
VERSION     NZ_CP127259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35637330
            Assembly: GCF_030285505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Bacteriocin-Producing Escherichia coli Q5 and Escherichia coli C41
            strains with probiotic properties: in silico, in vitro, in vivo
            studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17105)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Laboratory of Metagenome Analysis, Skolkovo
            Institute of Science and Technology, Bolshoy Boulevard 30, bld. 1,
            Moscow 121205, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2023
            Assembly Method                   :: Flye v. 2.9.1
            Assembly Name                     :: E_coli_Q5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 16:29:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,174
            CDSs (total)                      :: 5,058
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 178 of 290
            Pseudo Genes (incomplete)         :: 140 of 290
            Pseudo Genes (internal stop)      :: 35 of 290
            Pseudo Genes (multiple problems)  :: 55 of 290
            Pseudo Genes (short protein)      :: 3 of 290
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Q5"
                     /isolation_source="feces"
                     /host="Coturnix coturnix"
                     /culture_collection="VKM:B-3706D"
                     /db_xref="taxon:562"
                     /plasmid="pQ504"
                     /geo_loc_name="Russia: Perm krai"
                     /collection_date="2020-07"
                     /collected_by="Kuznetsova Marina, Mihailovskaya Veronika"
     gene            complement(725..865)
                     /gene="crl"
                     /locus_tag="QQ972_RS25420"
                     /old_locus_tag="QQ972_25425"
     CDS             complement(725..865)
                     /gene="crl"
                     /locus_tag="QQ972_RS25420"
                     /old_locus_tag="QQ972_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            985..1509
                     /gene="cui"
                     /locus_tag="QQ972_RS25425"
                     /old_locus_tag="QQ972_25430"
     CDS             985..1509
                     /gene="cui"
                     /locus_tag="QQ972_RS25425"
                     /old_locus_tag="QQ972_25430"
                     /inference="COORDINATES: protein motif:HMM:NF015795.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            complement(1537..3426)
                     /gene="cya"
                     /locus_tag="QQ972_RS25430"
                     /old_locus_tag="QQ972_25435"
     CDS             complement(1537..3426)
                     /gene="cya"
                     /locus_tag="QQ972_RS25430"
                     /old_locus_tag="QQ972_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_106372970.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKEAEAKANNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            3652..4089
                     /locus_tag="QQ972_RS25435"
                     /old_locus_tag="QQ972_25440"
     CDS             3652..4089
                     /locus_tag="QQ972_RS25435"
                     /old_locus_tag="QQ972_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            4170..4598
                     /locus_tag="QQ972_RS25440"
                     /old_locus_tag="QQ972_25445"
     CDS             4170..4598
                     /locus_tag="QQ972_RS25440"
                     /old_locus_tag="QQ972_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6226..6459)
                     /locus_tag="QQ972_RS25445"
                     /old_locus_tag="QQ972_25450"
     CDS             complement(6226..6459)
                     /locus_tag="QQ972_RS25445"
                     /old_locus_tag="QQ972_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6466..6996)
                     /locus_tag="QQ972_RS25450"
                     /old_locus_tag="QQ972_25455"
     CDS             complement(6466..6996)
                     /locus_tag="QQ972_RS25450"
                     /old_locus_tag="QQ972_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<7034..7465)
                     /locus_tag="QQ972_RS25455"
                     /old_locus_tag="QQ972_25460"
                     /pseudo=""
     CDS             complement(<7034..7465)
                     /locus_tag="QQ972_RS25455"
                     /old_locus_tag="QQ972_25460"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7674..7997)
                     /gene="mobC"
                     /locus_tag="QQ972_RS25460"
                     /old_locus_tag="QQ972_25465"
     CDS             complement(7674..7997)
                     /gene="mobC"
                     /locus_tag="QQ972_RS25460"
                     /old_locus_tag="QQ972_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032286519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="mobC"
     gene            8062..8253
                     /locus_tag="QQ972_RS25465"
                     /old_locus_tag="QQ972_25470"
     CDS             8062..8253
                     /locus_tag="QQ972_RS25465"
                     /old_locus_tag="QQ972_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9277..9417)
                     /gene="crl"
                     /locus_tag="QQ972_RS25470"
                     /old_locus_tag="QQ972_25475"
     CDS             complement(9277..9417)
                     /gene="crl"
                     /locus_tag="QQ972_RS25470"
                     /old_locus_tag="QQ972_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            9537..10061
                     /gene="cui"
                     /locus_tag="QQ972_RS25475"
                     /old_locus_tag="QQ972_25480"
     CDS             9537..10061
                     /gene="cui"
                     /locus_tag="QQ972_RS25475"
                     /old_locus_tag="QQ972_25480"
                     /inference="COORDINATES: protein motif:HMM:NF015795.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            complement(10089..11978)
                     /gene="cya"
                     /locus_tag="QQ972_RS25480"
                     /old_locus_tag="QQ972_25485"
     CDS             complement(10089..11978)
                     /gene="cya"
                     /locus_tag="QQ972_RS25480"
                     /old_locus_tag="QQ972_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_106372970.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKEAEAKANNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            12204..12641
                     /locus_tag="QQ972_RS25485"
                     /old_locus_tag="QQ972_25490"
     CDS             12204..12641
                     /locus_tag="QQ972_RS25485"
                     /old_locus_tag="QQ972_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            12722..13150
                     /locus_tag="QQ972_RS25490"
                     /old_locus_tag="QQ972_25495"
     CDS             12722..13150
                     /locus_tag="QQ972_RS25490"
                     /old_locus_tag="QQ972_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(14778..15011)
                     /locus_tag="QQ972_RS25495"
                     /old_locus_tag="QQ972_25500"
     CDS             complement(14778..15011)
                     /locus_tag="QQ972_RS25495"
                     /old_locus_tag="QQ972_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(15018..15548)
                     /locus_tag="QQ972_RS25500"
                     /old_locus_tag="QQ972_25505"
     CDS             complement(15018..15548)
                     /locus_tag="QQ972_RS25500"
                     /old_locus_tag="QQ972_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<15586..16017)
                     /locus_tag="QQ972_RS25505"
                     /old_locus_tag="QQ972_25510"
                     /pseudo=""
     CDS             complement(<15586..16017)
                     /locus_tag="QQ972_RS25505"
                     /old_locus_tag="QQ972_25510"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(16226..16549)
                     /gene="mobC"
                     /locus_tag="QQ972_RS25510"
                     /old_locus_tag="QQ972_25515"
     CDS             complement(16226..16549)
                     /gene="mobC"
                     /locus_tag="QQ972_RS25510"
                     /old_locus_tag="QQ972_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032286519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="mobC"
     gene            16614..16805
                     /locus_tag="QQ972_RS25515"
                     /old_locus_tag="QQ972_25520"
     CDS             16614..16805
                     /locus_tag="QQ972_RS25515"
                     /old_locus_tag="QQ972_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(8339..8426)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(16891..16978)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          10..442
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.632"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8449..8994
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.353"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6144..7684)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.09"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(14696..16236)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.09"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggtaccggc gcactcaaag gcggtaatac ggttatccac agaatcaggg gataaggccg
       61 gaaagaacat gtgagcaaaa aggcaaaggc caacgaaagg ccgcgccgct ggcgtttttc
      121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      301 gcgctttctc atagctcacg ctgttggtat ctcggcttgg tgtaggtcgt tcgctccaag
      361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      421 cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac tggcagcagc cactggtaac
      481 cggattagca gagcgatgat ggcacaaacg gtgctacaga gttcttgaag tagtggcccg
      541 actacggcta cactgaaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      601 tggttaagca gttccccaac tgacctaacc ttcgatcaaa ccgcctcccc aggcggtttt
      661 ttcgtttaca ggcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tccttttaac
      721 ttttctactg aaccgcgatc cccgtcagtt tagaagagga ggatggtgcg acagtccctc
      781 cctgaacatc gcggatgtaa ttagcttggc agcctgatag caaagccatc aagacaagca
      841 tccctcctga taatatcatt ttcatatatt ccctcattaa cacatacccc taatcttagg
      901 atgaccatta cttgtacagc atgatatgct tatacacatc tacaactaaa ttaataataa
      961 tgaaaagctt ggagagttat accaatgaga aaagagcaca ctgacaacaa agatttaaac
     1021 aggacgcttt ttttgattac ttttgcaggc ataacaccat taatcattat ttttataact
     1081 tatacatcaa accccaagtt ctatttaatt agcatcattt ttgataacac acaaaatata
     1141 ccttcaataa tttccgcata caatcctgtg atgacaaagg tgatggatat ctacgggaaa
     1201 agcgctccct tactggcact tattgctttt actctccaac tccgcgacag aaagctagag
     1261 acaatagcca acagagaaaa actaattact gccagcatat ttagtccatt tttttatgct
     1321 ttttatgcat atttctttct gtggaataat ttcgaattaa ccactgcagg taggactgtc
     1381 agatggatgt ccgataatga ttttactttg tttatttttt acgcctgcct ctatttttgc
     1441 tctttcttta tgacttatgc tctgtgttat attccggttg tgtcatataa actctggaaa
     1501 gaaaggtagt cttcttgact acctttcgac tctaggttaa tacgcagggc gaataatttc
     1561 gttatttaac ctgtctataa atttgtcatc aatcaatgct ccaataaaac tggctataat
     1621 aataccgata atccctacga ctgcagctga tgcccccaca gcaagcaggt aagctccaag
     1681 ggtggcagaa aaaatagcaa gagccacagc agaagcaata ccactgacaa cccaggactc
     1741 gacctcccgc attaacggcc cccagtttcc ggtctgatat ccctctatac tcttctcccg
     1801 gacgttattt gccttcatca cataatctgc ggctttaaat gtcttaccca gcgcggcaaa
     1861 cttattcccc atatcctctg catcaaatgc tttccaggca ttcacaatga catccctgtc
     1921 tgcagcatta attttaagat caggattagc catcagttta ttgacagaag ccatcgcctc
     1981 atcataacta cggatcgtct taccttgaaa gtttttgata ttacctgcta tctcacgaga
     2041 aagagcttta tatttatcgc caagatattc tccgacctta tcacccacac taatgataat
     2101 ttcactggct tttgtcagaa cagctttctc gttatttgct tttgcttcag cttcctttct
     2161 ggctttttca gcttcagctt ttgcttttgc ttcagcagcc gctttttcag cttcagcttt
     2221 cgcctttgca tcagcttttg ctttttctgc tgccgccttg ttcttttctg cctgctgttt
     2281 ccttgcagtc agtccggaaa cctgtgcctg cagcgcatta atatcactga ccagtaaatc
     2341 atttttgttc cgggcatcat catatgctct ctgagcatca cgaatacggt gataagaatg
     2401 gttcttaaat acttttttta gttcttcaac ctctttctgt ttttgtttca taaggcttaa
     2461 taatggtacc tgttgtttct tcttctccgc tattttaaga tttacgtcgt taatggcttt
     2521 cacggtctca ttaataatcc ggttcgccgc atcaacatcc ttctgtgagg ccgacattcc
     2581 ggatgatatc tggttgagaa tgagcgccaa attaaccgca tccaccaccc gttcagtaac
     2641 tcgccccata tcttttttca gatttaccgg gaaatcctga tatgccagac tccaggctgc
     2701 aacaatctgt ctgacagcct ctccctgttt tgccagtgga agggaatcga aagcctgccg
     2761 cattgctttg tactctgctt tattcatcac ctcttcaggc aagagaacac cagtcacata
     2821 gccttttttg ataatccgga cacttttgat ggcgttatta accagcccta cattcactgg
     2881 accagcccct gtccagctgt ccttcgccac agcatgccat tctccggcgg gtttctgtcc
     2941 gttcacaaaa ctctccacag aagtttcgct tccgggagta cttgctcctg atgcattaag
     3001 gcggtgaccg gtgtctattt cgcccacagc cccaataaca ccagaagcgt tatatcccat
     3061 gaccagccct cccaaaccaa gctgcttggc ctgagtggag gttatcccct caatggtgac
     3121 ctgcataaca ccagaaggag caatacttcc cagaatcatt tttgcttcag gattcatttt
     3181 tctcgcagca atcagcatat tcaccacttt ctgtttaagt ttctgatcat tctgaatggc
     3241 agaaatctgc tgactcacag aattgctact gtttgcgtta tctccgcgct ggcctccact
     3301 atttccatgc ataccaccgc ctggagctgg accaccactt tcagaactcc agccagtacc
     3361 gtcaccatac ccaccgtaat taaaccccgg catattcatt cctctttgac ttttaaaaca
     3421 aaccattaaa aacaaatact gtatacataa ccactggttt tatgtacagt acaaaagcta
     3481 cgccgctgca ttttccctgt caagactgtg gacttctatt tttgtgaaaa cgatcaaaaa
     3541 aacagtctct cacaccacgc gctattcccg ccggatacca caaaaaccgg cgcaaacatt
     3601 accgttctca gacctcatta tgttttactg aaaccatgag atgagacacc tatgggacac
     3661 tgtaacttta tggcatggca cacactccgg gacgcactga aaatgactgg aagatcgcgt
     3721 tcacagtttt accgtgatat gcgctcaggt cttgtcagtt accgtaccag ccaggacgga
     3781 cgaagggagt ttgaaaccag tgaactgatc cgggcatacg gcgaattaaa gcaaaatgag
     3841 acacaagaaa ggcacactgt gggacaggca gaaaatccac atgatcagca gacagaacgc
     3901 attctccggg aactgaatga gctgaaacaa tgcctgacgc tgatgcttga ggataaacag
     3961 gcacaggata tggatcgcag gcaccaggaa tcagaacggg aacagttaca gaatgagata
     4021 gcccagctca ggcaggcact ggaacaggaa aagaaacggg gattctggtc cagactcttc
     4081 ggtcactgaa ctcggtcaga ggctgatgat aagatagtat tcggataata actcaccgag
     4141 aataaatact ttaaggtaag gagacacgta tgaaacgttc tggtggcaca cgctatctgt
     4201 atcttatatc cctgataact gtggctgcag ccctcacggc ctgtaccccg aaaggcagtg
     4261 tggaacaata tacccgacat tatgtgtacg cttccgatga ccgttctgac cctaacttct
     4321 acaccaacaa ggccgacacc acacggatga tgattccgtt ctttcagcag ttcagggaga
     4381 tgggggagaa agacaaagca gccggagtat cagcagaaac ggcacagcaa cgcataaaag
     4441 aattccactc agaaaaattt cttcagtctc tcagaagcac aaccaccttt gcgggcagaa
     4501 aatatacgaa cagtgatatg ccatcaccgg aaaaaatgaa gctactggct gacacgattt
     4561 ctgcggttta tctcgatggc tacgagggaa gacagtaaat ggatttacca taatccctta
     4621 attgtacgca ccactgaaat gcgttcagcg cgatcacggc agcagacagg taaaaatgac
     4681 aacaaaccac ccgaaaaacc gccgcgatcg cacctgataa attttaacct catgcatatc
     4741 tatgcagcca tgcgaatcac gaacgaattg cctgcctgat gtaactgaaa cgggggtttt
     4801 ttcatatttt cggcaggttc aactcctgcc agatggaagc atcaggaggg aaatattact
     4861 gtacgtgaca gaactcatca ccgtacacac aggtatcatg ctccagaaaa aggaaacaat
     4921 tgcctgatac cagaaatcgc gttacaacac ttctgagcgc gtttctgaca gtctccatca
     4981 actgagctga atgttcgtca cacaacatcg aaacgtgctc accatccgcg accaacgccc
     5041 aatgaaactc tctcttccgg aagcgcccaa ttcgaaaaat aaccggtaga cctcctgtct
     5101 gtctcaggat tctggcacag taatcaggga acataacttt acagcgcccc ctgacctgca
     5161 tcgaaactaa aaaccgccgg aagaagtttt ttaaaatggc gaaccagaca tccacaactc
     5221 tcgacacaga gcccttgctc agctaaaaat ttacaggcgc gtttctggtg aattgttcgt
     5281 tgatgtagaa tgggttagtc gcatcaccgc cagaaacgcg ctcataagac agaacttcac
     5341 tgcctttata gttactactc aaaacaatat aaaacagaga gaaaaagaca aaaaaaagtc
     5401 cctcaaggag cagggactgt taattgcgtc tgaatataag aaaaaattag aagaattcac
     5461 tttcagctta agaataacgg tacagagcaa taacaatcaa caaaaatgat cagattttac
     5521 tttctgttat tttgtctttt ttgtaaaaaa caaaaaaata accaggaaaa ttcctggcat
     5581 caatattctt gtttatagtt tgttttatgg ttaatcttat tactctccag aggtgaccaa
     5641 caccaggata tttttttgct ctgtatcact cagcccctcc gtcaggtggg gcttcctttt
     5701 ttcacaccca tgaaaaaagc cctcacggca ggaaatcagt gagggcgtaa ggtaaggact
     5761 gaatcgatgg atagtatgtc tagtcctgac ttttgcatat cagaatataa ttcgcagact
     5821 tcaaatatgc aaaaaaaata tgcaaccaca tgtaagaact caatattaac acatgagaga
     5881 acgcgcatct ccgacccctt ccagattcgc tgtgtccaca tcggtattac aaacatatcg
     5941 gagatacgca attatatttt ttacataacc cgcgcattcc cgcaagttca ttttcagaaa
     6001 aatacctgtc ctctttacaa aaaaaacagc aacaacctgt acaaaaatca ataaaccgca
     6061 agaacgactg gttatattac caataggcag cctgacattt ctggaaaata ttctgtttac
     6121 gactcaaccg gactttcagt caccatccca gcgaagggcc atccagcttc cgctcccgtt
     6181 ccagagataa ctcatgttcg cgttcctgca tcagccgttc gtcccttatc tcctgttctc
     6241 gctgtataac tggctcaagc gctctgtccg ctcgctcaag tgctgcacct gctgactcaa
     6301 cttcatgacc cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa acattttctg
     6361 caattccacg aaggcgctct cccattcgtt cagccgctgc atatagtcct gttgcagttg
     6421 ctctaatgcg ttcagcaaat gtttttccag ctccgtcatg ctctgtcact cctgtaccag
     6481 tctcactgca ttcagtttgc cgttgtcctc cgtccagttc gtttcggctt tcatcccctt
     6541 cggcaacaca agaaaacgcc cgttgctgtc ctcgtgataa cgcacgcccc atgtcttcgc
     6601 gttcagcttc tccagactct ctttctgctc gcggagaata ttcatgtttg catttatctt
     6661 catgccctga taccacagca gactcccggt cgtggcaaac atcagcactg agacgataat
     6721 cagccacagc cagcgtttca cgctcatttt cagtacggtt tgccagtgtg tatccatcgt
     6781 gctgatggtc gttttctgat atctctccag cagttctttc agggctgttg tatggtcgtt
     6841 gatagcgttc ctgattaaag attcgtttga ggtcagttcc ctgtttacag attcttcgag
     6901 cttcctgaac tcgtcgttca gcattttctc tgtagattcg gctctctctt tcagcttttt
     6961 ctcgaactcc gcgcccgtct gtaaaagatt gctcataaaa cgctcctttc agtctgatat
     7021 tcttccctcc gttcgggtct gcaatgctga tactgcttcg cgtcaccctg accacttcaa
     7081 tccctgcctc cgtgagcgcc tgaatcacat cctgacggcc ttttatctcc ccgacatgat
     7141 aaagagcgtc tatcccgcgt gtgacgctct ctgcaagcgc ctgtttcgtt tccggcaggt
     7201 tatcagggag agtcagtatc cggcggttct ccggggcgtt ggggtcatgc agcccgtaat
     7261 ggtgattaac cagcgtctgc caggcatcaa ttctcggcct gtctgcccgg tcgtagtacg
     7321 gctggaggcg ttttccgctc tgtagctcca tgttcggaat gacaaaattc agctcaagcc
     7381 gtcccttgtc ctggtgctcc acccacagga tgctgtactg atttttttcg agaccgggca
     7441 tcagtacacg ctcaaagctc gccatcactt tttcacgtcc tcccggcggc agctccttct
     7501 ccgcgaacga cagaacacct gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt
     7561 cccggacttc ttccggatta ccctgaagca ccgttgcccc ttcccggtta cgctcccttc
     7621 ccagcaggta atcaaccgga ccactgccac cactttttcc cctggcatga aacttaacta
     7681 tcatcccgcg tcccctgttc cctgacggcc agacgcagcc ggcgcagctc atccccgatg
     7741 gccatcagtg cggccaccac ctgaacccgg tcaccggaag accactgccc gctgttcacc
     7801 ttcctcgctg tctgattcag gttattcccg atggcggcca gctgacgcag taacggcggt
     7861 gccagtgtcg gcagcttccc ggaacgggca accggctcac ccaggcagac ccgccgcatc
     7921 cataccgcca tttgtttacc ctcacagcgt tccagtaacc gggcatgttc atcatcagta
     7981 acccgtatcg tgagcatcct ctcgcgtttc atcggtatca ttacccccat gaacagaaat
     8041 cccccttaca cggaggcatc agtgacaaaa caggaaaaaa ccgcccttaa catggcccgc
     8101 tttatcagaa gccagacatt aacgctgctg gagaagctca acgaactgga cgcggatgaa
     8161 caggcagata tctgtgaatc gcttcacgac cacgccgatg agctttaccg cagctgcctc
     8221 gcacgcttcg gggatgacgg tgaaaaccac tgacacatgc agctcccgga gacgtacaca
     8281 actggtctgt gaatggatgc cgagagcaga caagcccgtc agggcgcgtc agcaggtttt
     8341 agcgggtgtc ggggcgcagc cctgacccag tcacgtagcg atagcggagt gtatactggc
     8401 ttaactatgc ggcatcagtg cggattgtat gaaaagtgca ccatagccga tgtgaaatgc
     8461 cgcacagatg cgtaaggaga aaatgcaact tccgacactc ttccgcttcc tcgctcactg
     8521 actcgctacg ctcggtcgtt cggctgcggc gagcggtacc ggcgcactca aaggcggtaa
     8581 tacggttatc cacagaatca ggggataagg ccggaaagaa catgtgagca aaaaggcaaa
     8641 ggccaacgaa aggccgcgcc gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8701 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8761 gcgtttcccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt
     8881 atctcggctt ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaaggcacg
     9001 ccttaacgcc actggcagca gccactggta accggattag cagagcgatg atggcacaaa
     9061 cggtgctaca gagttcttga agtagtggcc cgactacggc tacactgaaa ggacagtatt
     9121 tggtatctgc gctctgctga agccagttac cttggttaag cagttcccca actgacctaa
     9181 ccttcgatca aaccgcctcc ccaggcggtt ttttcgttta caggcagcag attacgcgca
     9241 gaaaaaaagg atctcaagaa gatcctttta acttttctac tgaaccgcga tccccgtcag
     9301 tttagaagag gaggatggtg cgacagtccc tccctgaaca tcgcggatgt aattagcttg
     9361 gcagcctgat agcaaagcca tcaagacaag catccctcct gataatatca ttttcatata
     9421 ttccctcatt aacacatacc cctaatctta ggatgaccat tacttgtaca gcatgatatg
     9481 cttatacaca tctacaacta aattaataat aatgaaaagc ttggagagtt ataccaatga
     9541 gaaaagagca cactgacaac aaagatttaa acaggacgct ttttttgatt acttttgcag
     9601 gcataacacc attaatcatt atttttataa cttatacatc aaaccccaag ttctatttaa
     9661 ttagcatcat ttttgataac acacaaaata taccttcaat aatttccgca tacaatcctg
     9721 tgatgacaaa ggtgatggat atctacggga aaagcgctcc cttactggca cttattgctt
     9781 ttactctcca actccgcgac agaaagctag agacaatagc caacagagaa aaactaatta
     9841 ctgccagcat atttagtcca tttttttatg ctttttatgc atatttcttt ctgtggaata
     9901 atttcgaatt aaccactgca ggtaggactg tcagatggat gtccgataat gattttactt
     9961 tgtttatttt ttacgcctgc ctctattttt gctctttctt tatgacttat gctctgtgtt
    10021 atattccggt tgtgtcatat aaactctgga aagaaaggta gtcttcttga ctacctttcg
    10081 actctaggtt aatacgcagg gcgaataatt tcgttattta acctgtctat aaatttgtca
    10141 tcaatcaatg ctccaataaa actggctata ataataccga taatccctac gactgcagct
    10201 gatgccccca cagcaagcag gtaagctcca agggtggcag aaaaaatagc aagagccaca
    10261 gcagaagcaa taccactgac aacccaggac tcgacctccc gcattaacgg cccccagttt
    10321 ccggtctgat atccctctat actcttctcc cggacgttat ttgccttcat cacataatct
    10381 gcggctttaa atgtcttacc cagcgcggca aacttattcc ccatatcctc tgcatcaaat
    10441 gctttccagg cattcacaat gacatccctg tctgcagcat taattttaag atcaggatta
    10501 gccatcagtt tattgacaga agccatcgcc tcatcataac tacggatcgt cttaccttga
    10561 aagtttttga tattacctgc tatctcacga gaaagagctt tatatttatc gccaagatat
    10621 tctccgacct tatcacccac actaatgata atttcactgg cttttgtcag aacagctttc
    10681 tcgttatttg cttttgcttc agcttccttt ctggcttttt cagcttcagc ttttgctttt
    10741 gcttcagcag ccgctttttc agcttcagct ttcgcctttg catcagcttt tgctttttct
    10801 gctgccgcct tgttcttttc tgcctgctgt ttccttgcag tcagtccgga aacctgtgcc
    10861 tgcagcgcat taatatcact gaccagtaaa tcatttttgt tccgggcatc atcatatgct
    10921 ctctgagcat cacgaatacg gtgataagaa tggttcttaa atactttttt tagttcttca
    10981 acctctttct gtttttgttt cataaggctt aataatggta cctgttgttt cttcttctcc
    11041 gctattttaa gatttacgtc gttaatggct ttcacggtct cattaataat ccggttcgcc
    11101 gcatcaacat ccttctgtga ggccgacatt ccggatgata tctggttgag aatgagcgcc
    11161 aaattaaccg catccaccac ccgttcagta actcgcccca tatctttttt cagatttacc
    11221 gggaaatcct gatatgccag actccaggct gcaacaatct gtctgacagc ctctccctgt
    11281 tttgccagtg gaagggaatc gaaagcctgc cgcattgctt tgtactctgc tttattcatc
    11341 acctcttcag gcaagagaac accagtcaca tagccttttt tgataatccg gacacttttg
    11401 atggcgttat taaccagccc tacattcact ggaccagccc ctgtccagct gtccttcgcc
    11461 acagcatgcc attctccggc gggtttctgt ccgttcacaa aactctccac agaagtttcg
    11521 cttccgggag tacttgctcc tgatgcatta aggcggtgac cggtgtctat ttcgcccaca
    11581 gccccaataa caccagaagc gttatatccc atgaccagcc ctcccaaacc aagctgcttg
    11641 gcctgagtgg aggttatccc ctcaatggtg acctgcataa caccagaagg agcaatactt
    11701 cccagaatca tttttgcttc aggattcatt tttctcgcag caatcagcat attcaccact
    11761 ttctgtttaa gtttctgatc attctgaatg gcagaaatct gctgactcac agaattgcta
    11821 ctgtttgcgt tatctccgcg ctggcctcca ctatttccat gcataccacc gcctggagct
    11881 ggaccaccac tttcagaact ccagccagta ccgtcaccat acccaccgta attaaacccc
    11941 ggcatattca ttcctctttg acttttaaaa caaaccatta aaaacaaata ctgtatacat
    12001 aaccactggt tttatgtaca gtacaaaagc tacgccgctg cattttccct gtcaagactg
    12061 tggacttcta tttttgtgaa aacgatcaaa aaaacagtct ctcacaccac gcgctattcc
    12121 cgccggatac cacaaaaacc ggcgcaaaca ttaccgttct cagacctcat tatgttttac
    12181 tgaaaccatg agatgagaca cctatgggac actgtaactt tatggcatgg cacacactcc
    12241 gggacgcact gaaaatgact ggaagatcgc gttcacagtt ttaccgtgat atgcgctcag
    12301 gtcttgtcag ttaccgtacc agccaggacg gacgaaggga gtttgaaacc agtgaactga
    12361 tccgggcata cggcgaatta aagcaaaatg agacacaaga aaggcacact gtgggacagg
    12421 cagaaaatcc acatgatcag cagacagaac gcattctccg ggaactgaat gagctgaaac
    12481 aatgcctgac gctgatgctt gaggataaac aggcacagga tatggatcgc aggcaccagg
    12541 aatcagaacg ggaacagtta cagaatgaga tagcccagct caggcaggca ctggaacagg
    12601 aaaagaaacg gggattctgg tccagactct tcggtcactg aactcggtca gaggctgatg
    12661 ataagatagt attcggataa taactcaccg agaataaata ctttaaggta aggagacacg
    12721 tatgaaacgt tctggtggca cacgctatct gtatcttata tccctgataa ctgtggctgc
    12781 agccctcacg gcctgtaccc cgaaaggcag tgtggaacaa tatacccgac attatgtgta
    12841 cgcttccgat gaccgttctg accctaactt ctacaccaac aaggccgaca ccacacggat
    12901 gatgattccg ttctttcagc agttcaggga gatgggggag aaagacaaag cagccggagt
    12961 atcagcagaa acggcacagc aacgcataaa agaattccac tcagaaaaat ttcttcagtc
    13021 tctcagaagc acaaccacct ttgcgggcag aaaatatacg aacagtgata tgccatcacc
    13081 ggaaaaaatg aagctactgg ctgacacgat ttctgcggtt tatctcgatg gctacgaggg
    13141 aagacagtaa atggatttac cataatccct taattgtacg caccactgaa atgcgttcag
    13201 cgcgatcacg gcagcagaca ggtaaaaatg acaacaaacc acccgaaaaa ccgccgcgat
    13261 cgcacctgat aaattttaac ctcatgcata tctatgcagc catgcgaatc acgaacgaat
    13321 tgcctgcctg atgtaactga aacgggggtt ttttcatatt ttcggcaggt tcaactcctg
    13381 ccagatggaa gcatcaggag ggaaatatta ctgtacgtga cagaactcat caccgtacac
    13441 acaggtatca tgctccagaa aaaggaaaca attgcctgat accagaaatc gcgttacaac
    13501 acttctgagc gcgtttctga cagtctccat caactgagct gaatgttcgt cacacaacat
    13561 cgaaacgtgc tcaccatccg cgaccaacgc ccaatgaaac tctctcttcc ggaagcgccc
    13621 aattcgaaaa ataaccggta gacctcctgt ctgtctcagg attctggcac agtaatcagg
    13681 gaacataact ttacagcgcc ccctgacctg catcgaaact aaaaaccgcc ggaagaagtt
    13741 ttttaaaatg gcgaaccaga catccacaac tctcgacaca gagcccttgc tcagctaaaa
    13801 atttacaggc gcgtttctgg tgaattgttc gttgatgtag aatgggttag tcgcatcacc
    13861 gccagaaacg cgctcataag acagaacttc actgccttta tagttactac tcaaaacaat
    13921 ataaaacaga gagaaaaaga caaaaaaaag tccctcaagg agcagggact gttaattgcg
    13981 tctgaatata agaaaaaatt agaagaattc actttcagct taagaataac ggtacagagc
    14041 aataacaatc aacaaaaatg atcagatttt actttctgtt attttgtctt ttttgtaaaa
    14101 aacaaaaaaa taaccaggaa aattcctggc atcaatattc ttgtttatag tttgttttat
    14161 ggttaatctt attactctcc agaggtgacc aacaccagga tatttttttg ctctgtatca
    14221 ctcagcccct ccgtcaggtg gggcttcctt ttttcacacc catgaaaaaa gccctcacgg
    14281 caggaaatca gtgagggcgt aaggtaagga ctgaatcgat ggatagtatg tctagtcctg
    14341 acttttgcat atcagaatat aattcgcaga cttcaaatat gcaaaaaaaa tatgcaacca
    14401 catgtaagaa ctcaatatta acacatgaga gaacgcgcat ctccgacccc ttccagattc
    14461 gctgtgtcca catcggtatt acaaacatat cggagatacg caattatatt ttttacataa
    14521 cccgcgcatt cccgcaagtt cattttcaga aaaatacctg tcctctttac aaaaaaaaca
    14581 gcaacaacct gtacaaaaat caataaaccg caagaacgac tggttatatt accaataggc
    14641 agcctgacat ttctggaaaa tattctgttt acgactcaac cggactttca gtcaccatcc
    14701 cagcgaaggg ccatccagct tccgctcccg ttccagagat aactcatgtt cgcgttcctg
    14761 catcagccgt tcgtccctta tctcctgttc tcgctgtata actggctcaa gcgctctgtc
    14821 cgctcgctca agtgctgcac ctgctgactc aacttcatga cccgctcgtt cagcatcgcg
    14881 ttgtcccgtt gcgtaagcga aaacattttc tgcaattcca cgaaggcgct ctcccattcg
    14941 ttcagccgct gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa atgtttttcc
    15001 agctccgtca tgctctgtca ctcctgtacc agtctcactg cattcagttt gccgttgtcc
    15061 tccgtccagt tcgtttcggc tttcatcccc ttcggcaaca caagaaaacg cccgttgctg
    15121 tcctcgtgat aacgcacgcc ccatgtcttc gcgttcagct tctccagact ctctttctgc
    15181 tcgcggagaa tattcatgtt tgcatttatc ttcatgccct gataccacag cagactcccg
    15241 gtcgtggcaa acatcagcac tgagacgata atcagccaca gccagcgttt cacgctcatt
    15301 ttcagtacgg tttgccagtg tgtatccatc gtgctgatgg tcgttttctg atatctctcc
    15361 agcagttctt tcagggctgt tgtatggtcg ttgatagcgt tcctgattaa agattcgttt
    15421 gaggtcagtt ccctgtttac agattcttcg agcttcctga actcgtcgtt cagcattttc
    15481 tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt ctgtaaaaga
    15541 ttgctcataa aacgctcctt tcagtctgat attcttccct ccgttcgggt ctgcaatgct
    15601 gatactgctt cgcgtcaccc tgaccacttc aatccctgcc tccgtgagcg cctgaatcac
    15661 atcctgacgg ccttttatct ccccgacatg ataaagagcg tctatcccgc gtgtgacgct
    15721 ctctgcaagc gcctgtttcg tttccggcag gttatcaggg agagtcagta tccggcggtt
    15781 ctccggggcg ttggggtcat gcagcccgta atggtgatta accagcgtct gccaggcatc
    15841 aattctcggc ctgtctgccc ggtcgtagta cggctggagg cgttttccgc tctgtagctc
    15901 catgttcgga atgacaaaat tcagctcaag ccgtcccttg tcctggtgct ccacccacag
    15961 gatgctgtac tgattttttt cgagaccggg catcagtaca cgctcaaagc tcgccatcac
    16021 tttttcacgt cctcccggcg gcagctcctt ctccgcgaac gacagaacac ctgacgtgta
    16081 tttcttcgca aatggcgtgg catcgatgag ttcccggact tcttccggat taccctgaag
    16141 caccgttgcc ccttcccggt tacgctccct tcccagcagg taatcaaccg gaccactgcc
    16201 accacttttt cccctggcat gaaacttaac tatcatcccg cgtcccctgt tccctgacgg
    16261 ccagacgcag ccggcgcagc tcatccccga tggccatcag tgcggccacc acctgaaccc
    16321 ggtcaccgga agaccactgc ccgctgttca ccttcctcgc tgtctgattc aggttattcc
    16381 cgatggcggc cagctgacgc agtaacggcg gtgccagtgt cggcagcttc ccggaacggg
    16441 caaccggctc acccaggcag acccgccgca tccataccgc catttgttta ccctcacagc
    16501 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctcgcgtt
    16561 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacaa
    16621 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgctgc
    16681 tggagaagct caacgaactg gacgcggatg aacaggcaga tatctgtgaa tcgcttcacg
    16741 accacgccga tgagctttac cgcagctgcc tcgcacgctt cggggatgac ggtgaaaacc
    16801 actgacacat gcagctcccg gagacgtaca caactggtct gtgaatggat gccgagagca
    16861 gacaagcccg tcagggcgcg tcagcaggtt ttagcgggtg tcggggcgca gccctgaccc
    16921 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag tgcggattgt
    16981 atgaaaagtg caccatagcc gatgtgaaat gccgcacaga tgcgtaagga gaaaatgcaa
    17041 cttccgacac tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg
    17101 gcgag
//
