LOCUS       NZ_CP127258             7790 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Escherichia coli strain Q5 plasmid pQ505, complete sequence.
ACCESSION   NZ_CP127258
VERSION     NZ_CP127258.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35637330
            Assembly: GCF_030285505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Bacteriocin-Producing Escherichia coli Q5 and Escherichia coli C41
            strains with probiotic properties: in silico, in vitro, in vivo
            studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Laboratory of Metagenome Analysis, Skolkovo
            Institute of Science and Technology, Bolshoy Boulevard 30, bld. 1,
            Moscow 121205, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2023
            Assembly Method                   :: Flye v. 2.9.1
            Assembly Name                     :: E_coli_Q5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 16:29:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,174
            CDSs (total)                      :: 5,058
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 178 of 290
            Pseudo Genes (incomplete)         :: 140 of 290
            Pseudo Genes (internal stop)      :: 35 of 290
            Pseudo Genes (multiple problems)  :: 55 of 290
            Pseudo Genes (short protein)      :: 3 of 290
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127258.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Q5"
                     /isolation_source="feces"
                     /host="Coturnix coturnix"
                     /culture_collection="VKM:B-3706D"
                     /db_xref="taxon:562"
                     /plasmid="pQ505"
                     /geo_loc_name="Russia: Perm krai"
                     /collection_date="2020-07"
                     /collected_by="Kuznetsova Marina, Mihailovskaya Veronika"
     gene            complement(join(7565..7790,1..794))
                     /locus_tag="QQ972_RS25390"
                     /old_locus_tag="QQ972_25395"
     CDS             complement(join(7565..7790,1..794))
                     /locus_tag="QQ972_RS25390"
                     /old_locus_tag="QQ972_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023148269.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVNQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1250..2791)
                     /locus_tag="QQ972_RS25395"
                     /old_locus_tag="QQ972_25400"
     CDS             complement(1250..2791)
                     /locus_tag="QQ972_RS25395"
                     /old_locus_tag="QQ972_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_285985052.1"
                     /translation="MASYHCTVKTGAKGAALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPLGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGIKRTPEVHLGPVHAA
                     NLNEGQIAAIQEHRAARQQERAARNPAPERQQELEQKQKIRTVERSLELLAQYRKVMKT
                     VIQGEERLARLGDANPDALKEHKLLQNAKAKKESLSEWSRRIYEGARYLDKLGRNVVSA
                     QRELRELQEQRNALNGIRGLFRGADKREIDARILEQESVLETAEREENEFRYKLQQAES
                     EWKEKYAAFQRTEGYKYVDPTGLDKYREREILAAVSLENTRQKVAEEISVARSQMLSLE
                     PELNMSGDEKAQMRHELLAEMALEQQQQEEKALRIQQTWVREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            2963..3409
                     /locus_tag="QQ972_RS25400"
                     /old_locus_tag="QQ972_25405"
     CDS             2963..3409
                     /locus_tag="QQ972_RS25400"
                     /old_locus_tag="QQ972_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_021537414.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLAKDSA"
                     /name="conjugal transfer protein TraD"
     gene            complement(3670..4689)
                     /locus_tag="QQ972_RS25405"
                     /old_locus_tag="QQ972_25410"
     CDS             complement(3670..4689)
                     /locus_tag="QQ972_RS25405"
                     /old_locus_tag="QQ972_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023148269.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVNQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(5145..6686)
                     /locus_tag="QQ972_RS25410"
                     /old_locus_tag="QQ972_25415"
     CDS             complement(5145..6686)
                     /locus_tag="QQ972_RS25410"
                     /old_locus_tag="QQ972_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_285985052.1"
                     /translation="MASYHCTVKTGAKGAALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPLGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGIKRTPEVHLGPVHAA
                     NLNEGQIAAIQEHRAARQQERAARNPAPERQQELEQKQKIRTVERSLELLAQYRKVMKT
                     VIQGEERLARLGDANPDALKEHKLLQNAKAKKESLSEWSRRIYEGARYLDKLGRNVVSA
                     QRELRELQEQRNALNGIRGLFRGADKREIDARILEQESVLETAEREENEFRYKLQQAES
                     EWKEKYAAFQRTEGYKYVDPTGLDKYREREILAAVSLENTRQKVAEEISVARSQMLSLE
                     PELNMSGDEKAQMRHELLAEMALEQQQQEEKALRIQQTWVREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            6858..7304
                     /locus_tag="QQ972_RS25415"
                     /old_locus_tag="QQ972_25420"
     CDS             6858..7304
                     /locus_tag="QQ972_RS25415"
                     /old_locus_tag="QQ972_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_021537414.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLAKDSA"
                     /name="conjugal transfer protein TraD"
     Typing          complement(20..794)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3915..4689)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(1254..2791)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.085"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5149..6686)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.085"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccttgcgac cgcgtgctgc ctgaatttct ggcgtgtgcg tatcagccac atactgtgcg
       61 aaagtttccg gcgtgaagtt cctgtgcgtg tatttcgcaa tgctcttgcc aatagcccga
      121 cattcagcca gtgaaagcgg aacgggaagc gatgcattgt acatttcgac acgctggttc
      181 accgcttcaa gccactgtga gaacgctggc cagccctgac gaatcgccct gtaagcccat
      241 ttgcgcgtca tttcgaacag gtggcagttt cgccccatcc cgtaatgttt atcgatgcta
      301 cgacgctctg aggcgctcaa atcgagataa tctgccagtt catcgagggt ataggcgtcc
      361 tcgcgccact ccatcacctg ccattccagg tggaacggat ttttgcaaat caggccgctg
      421 tagttcacat cagcacaaag tttctcacac agcgaacgtt caaccgctgc ggcatatttc
      481 agcgccttaa ccgaggcatc aggcgcggtt ctcacggcga tgttgagtgc gtagagcagg
      541 tgagcgtgtc cgttcacagg atttttaacg gtgatgttcg gcgcgggggc gttccggtcg
      601 ctccagtcaa tcgcagcccc ctcgcggtca acgtcaaaaa ccagccagaa ctgcgcatga
      661 ggctggttct gctggatgta ccgcgcaaga agcgcacgcc ctttgccgga gatgcgaagg
      721 ccgaaagcga gatcatccgt gtgatagggg cggtggggta aattttcttc gaagtattga
      781 agcgcggcgc tcatgtgtta cgattccttc caggttatcg accgacctcg cccctgtatt
      841 ctcgtgacaa tcttggcgga gagacacgaa tttcaggggc gtttgttttt cagtcgctta
      901 cactaccccc aacaacaaaa cctgtcgagc cttcccgcaa aaccgatcca tttaccataa
      961 ctcccgttgt gcgcccccct taaaacgctc tcagacgctt tctgacgcaa aaaacgcaga
     1021 aatacaagga aacaccagga agaaccagaa aaacgcggca gaattgtttt taggaggctg
     1081 cataactatt cacgaaagca atgcgcgatc tgtttcgagc gttcagaagg gcgaacgggt
     1141 caaaacagca aactttctca tcagaaaacg gtgaacggga cgcggaattt tttcgcgttc
     1201 cgtgaaacgt tggcggggtt cggggcgcag tcccgatatt gctacatttt caacgctcca
     1261 tatcttgatc acgttcgtta ctacgagatg cctctctgac ccaggtttgc tgtatacgca
     1321 gcgctttttc ttcctgttgc tgctgttcca acgccatttc tgccagtaac tcatgtcgca
     1381 tttgagcttt ttcatctcca gacatattaa gttcaggctc cagagataac atctggctac
     1441 gggcaacgga tatttcttcg gcaacttttt gcctggtgtt ctctaaagag actgcggcca
     1501 gaatctcacg ttctctgtac ttgtccagcc ccgtaggatc aacgtactta tatccctcgg
     1561 tacgctgaaa agcagcgtat ttctctttcc actcggactc agcttgctgt agcttgtacc
     1621 tgaactcatt ctcttcacgt tcggcggttt ccagcacgct ttcttgctcc agaatacgcg
     1681 catcaatctc ccttttgtca gccccccgaa acaggccacg aatcccattc agagcattgc
     1741 gttgttcctg taactctcgt agctcccttt gtgcggaaac aacattacgc cccagtttgt
     1801 ctaaataccg tgccccttca tatattcgcc tgctccattc ggaaagagat tcttttttag
     1861 ctttcgcatt ctgaagcagc ttatgttctt tgagcgcatc ggggttggca tcccctaaac
     1921 gagcaagcct ttcctctccc tgaatgaccg ttttcataac cttgcggtac tgagccagta
     1981 actccagtga acgctcaact gtcctgattt tttgcttttg ctccagttcc tgttggcgct
     2041 ctggcgctgg gttgcgtgct gcgcgttctt gctgacgcgc cgccctgtgc tcctgaattg
     2101 ccgcgatctg cccctcgttc agattggcgg cgtgaacagg gccaagatga acttccggcg
     2161 tgcgcttgat gccctgttct ttcagcgaac gatgatctac gcgcgactcg tgaccgtgtc
     2221 gcgcaaggtg ctcattctgc acatcagccc agcgggaacg tagcgcaaca agggcggctt
     2281 ttcgctctcc ggcggtctgg ggaatactcg ccttttttgc gcctccccgc tccggtgatt
     2341 tgctgttggc gcgtttaaaa aattgctgcg gatcgcgttc aataccatca ttaatccgct
     2401 cgcaaaacat gatatgcgcg tgtggctgtt cctttccgtc gattgcacct agcggattgt
     2461 ggatcgcgta ctggtaggca tggcgatcgc caagttcctt ttgcacgaaa tcttcaacaa
     2521 gttctatccg ttgttcgcgt gtcagttcac gcggtaatgc gatctcaatt tcacggtatg
     2581 ccgtcccgtt ttttcgttca aattcatctg cggctttcca cagttctgcg ggatttttct
     2641 tcgcccatga tggcatgtta ccggatgacg aaaactccag atcctcccgc gatttgtagc
     2701 tcttatattc gccggatctg gaaatgtaat cagcatgttt taaggctgcg ccttttgctc
     2761 cggttttaac ggtgcaatgg tagcttgcca tctgcctttt agccctccgc aggagcgcac
     2821 agtttcttga tgaaatcgag aaactactaa gtgcgcatcc ctgaattaat cagggatgat
     2881 tttacggtct cacacgcagg caaacaaggt gatattatgc acaataacaa ctcacattaa
     2941 cggcaaaagt gcagaggtaa gaatgacaaa tcgtgaagag tggttaagtg caaaaattgc
     3001 ctacatcaac ggactgaaat caccgtcaga gcagcagaga ttacttgtgt tactggcaga
     3061 gaaaaaaaac cgaacaacga cggatgaaaa aacgttgtcc gcactgatac gggcagaaaa
     3121 aaccgcagag aaagcagccg cagcaaaagc cagggtaacg gcaatcatag cggcagaacg
     3181 gaaggcagcc gcaagggcag aacgaaaggc gcgggatcat gaactttaca aggccgcagg
     3241 cctcatgatt gtggcaggac tggtggacag caaaaccgga aaaccaaagt ttagtgctgc
     3301 cgaactggta ggcgcactcg caggaatcgc agagctaccg cgcaaccatc ccaaatggca
     3361 ggaatgggaa aaacgcggga aagagctact cgccaaagac agtgcgtaag agcctccgtt
     3421 cacgttccgg ttccctggtg cttcctcgct cattcgctcg ctacgctcgg tcatgagact
     3481 gcggcgagca cgaccacctc aatcatgcgg taagccgcac cattgctggg ctgagaggag
     3541 aaaaccgccg gaaagcccgc tgctcaaaaa gacgccgaaa ggcgctgtta tctgatatag
     3601 gcttgttcat gtccgcgaat ttaaccaacc attaacaaaa aacagggggt tttgttgctt
     3661 ctttgttaac taacatttaa ttatttgttt cttggttagc cattctccag aattatccat
     3721 ccagcgtcga atagtccggc tgctaacacc aaccgccact gcaatatctt ccgttgacgc
     3781 tcccaagata tacatatcaa gcgccctacg acgtttgtcg tcgttagcag tccaccgaac
     3841 agcggcgctt tttttccctt tgtccgactg attttctgac gaattggcct tcccgccctt
     3901 gcgaccgcgt gctgcctgaa tttctggcgt gtgcgtatca gccacatact gtgcgaaagt
     3961 ttccggcgtg aagttcctgt gcgtgtattt cgcaatgctc ttgccaatag cccgacattc
     4021 agccagtgaa agcggaacgg gaagcgatgc attgtacatt tcgacacgct ggttcaccgc
     4081 ttcaagccac tgtgagaacg ctggccagcc ctgacgaatc gccctgtaag cccatttgcg
     4141 cgtcatttcg aacaggtggc agtttcgccc catcccgtaa tgtttatcga tgctacgacg
     4201 ctctgaggcg ctcaaatcga gataatctgc cagttcatcg agggtatagg cgtcctcgcg
     4261 ccactccatc acctgccatt ccaggtggaa cggatttttg caaatcaggc cgctgtagtt
     4321 cacatcagca caaagtttct cacacagcga acgttcaacc gctgcggcat atttcagcgc
     4381 cttaaccgag gcatcaggcg cggttctcac ggcgatgttg agtgcgtaga gcaggtgagc
     4441 gtgtccgttc acaggatttt taacggtgat gttcggcgcg ggggcgttcc ggtcgctcca
     4501 gtcaatcgca gccccctcgc ggtcaacgtc aaaaaccagc cagaactgcg catgaggctg
     4561 gttctgctgg atgtaccgcg caagaagcgc acgccctttg ccggagatgc gaaggccgaa
     4621 agcgagatca tccgtgtgat aggggcggtg gggtaaattt tcttcgaagt attgaagcgc
     4681 ggcgctcatg tgttacgatt ccttccaggt tatcgaccga cctcgcccct gtattctcgt
     4741 gacaatcttg gcggagagac acgaatttca ggggcgtttg tttttcagtc gcttacacta
     4801 cccccaacaa caaaacctgt cgagccttcc cgcaaaaccg atccatttac cataactccc
     4861 gttgtgcgcc ccccttaaaa cgctctcaga cgctttctga cgcaaaaaac gcagaaatac
     4921 aaggaaacac caggaagaac cagaaaaacg cggcagaatt gtttttagga ggctgcataa
     4981 ctattcacga aagcaatgcg cgatctgttt cgagcgttca gaagggcgaa cgggtcaaaa
     5041 cagcaaactt tctcatcaga aaacggtgaa cgggacgcgg aattttttcg cgttccgtga
     5101 aacgttggcg gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct
     5161 tgatcacgtt cgttactacg agatgcctct ctgacccagg tttgctgtat acgcagcgct
     5221 ttttcttcct gttgctgctg ttccaacgcc atttctgcca gtaactcatg tcgcatttga
     5281 gctttttcat ctccagacat attaagttca ggctccagag ataacatctg gctacgggca
     5341 acggatattt cttcggcaac tttttgcctg gtgttctcta aagagactgc ggccagaatc
     5401 tcacgttctc tgtacttgtc cagccccgta ggatcaacgt acttatatcc ctcggtacgc
     5461 tgaaaagcag cgtatttctc tttccactcg gactcagctt gctgtagctt gtacctgaac
     5521 tcattctctt cacgttcggc ggtttccagc acgctttctt gctccagaat acgcgcatca
     5581 atctcccttt tgtcagcccc ccgaaacagg ccacgaatcc cattcagagc attgcgttgt
     5641 tcctgtaact ctcgtagctc cctttgtgcg gaaacaacat tacgccccag tttgtctaaa
     5701 taccgtgccc cttcatatat tcgcctgctc cattcggaaa gagattcttt tttagctttc
     5761 gcattctgaa gcagcttatg ttctttgagc gcatcggggt tggcatcccc taaacgagca
     5821 agcctttcct ctccctgaat gaccgttttc ataaccttgc ggtactgagc cagtaactcc
     5881 agtgaacgct caactgtcct gattttttgc ttttgctcca gttcctgttg gcgctctggc
     5941 gctgggttgc gtgctgcgcg ttcttgctga cgcgccgccc tgtgctcctg aattgccgcg
     6001 atctgcccct cgttcagatt ggcggcgtga acagggccaa gatgaacttc cggcgtgcgc
     6061 ttgatgccct gttctttcag cgaacgatga tctacgcgcg actcgtgacc gtgtcgcgca
     6121 aggtgctcat tctgcacatc agcccagcgg gaacgtagcg caacaagggc ggcttttcgc
     6181 tctccggcgg tctggggaat actcgccttt tttgcgcctc cccgctccgg tgatttgctg
     6241 ttggcgcgtt taaaaaattg ctgcggatcg cgttcaatac catcattaat ccgctcgcaa
     6301 aacatgatat gcgcgtgtgg ctgttccttt ccgtcgattg cacctagcgg attgtggatc
     6361 gcgtactggt aggcatggcg atcgccaagt tccttttgca cgaaatcttc aacaagttct
     6421 atccgttgtt cgcgtgtcag ttcacgcggt aatgcgatct caatttcacg gtatgccgtc
     6481 ccgttttttc gttcaaattc atctgcggct ttccacagtt ctgcgggatt tttcttcgcc
     6541 catgatggca tgttaccgga tgacgaaaac tccagatcct cccgcgattt gtagctctta
     6601 tattcgccgg atctggaaat gtaatcagca tgttttaagg ctgcgccttt tgctccggtt
     6661 ttaacggtgc aatggtagct tgccatctgc cttttagccc tccgcaggag cgcacagttt
     6721 cttgatgaaa tcgagaaact actaagtgcg catccctgaa ttaatcaggg atgattttac
     6781 ggtctcacac gcaggcaaac aaggtgatat tatgcacaat aacaactcac attaacggca
     6841 aaagtgcaga ggtaagaatg acaaatcgtg aagagtggtt aagtgcaaaa attgcctaca
     6901 tcaacggact gaaatcaccg tcagagcagc agagattact tgtgttactg gcagagaaaa
     6961 aaaaccgaac aacgacggat gaaaaaacgt tgtccgcact gatacgggca gaaaaaaccg
     7021 cagagaaagc agccgcagca aaagccaggg taacggcaat catagcggca gaacggaagg
     7081 cagccgcaag ggcagaacga aaggcgcggg atcatgaact ttacaaggcc gcaggcctca
     7141 tgattgtggc aggactggtg gacagcaaaa ccggaaaacc aaagtttagt gctgccgaac
     7201 tggtaggcgc actcgcagga atcgcagagc taccgcgcaa ccatcccaaa tggcaggaat
     7261 gggaaaaacg cgggaaagag ctactcgcca aagacagtgc gtaagagcct ccgttcacgt
     7321 tccggttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     7381 gagcacgacc acctcaatca tgcggtaagc cgcaccattg ctgggctgag aggagaaaac
     7441 cgccggaaag cccgctgctc aaaaagacgc cgaaaggcgc tgttatctga tataggcttg
     7501 ttcatgtccg cgaatttaac caaccattaa caaaaaacag ggggttttgt tgcttctttg
     7561 ttaactaaca tttaattatt tgtttcttgg ttagccattc tccagaatta tccatccagc
     7621 gtcgaatagt ccggctgcta acaccaaccg ccactgcaat atcttccgtt gacgctccca
     7681 agatatacat atcaagcgcc ctacgacgtt tgtcgtcgtt agcagtccac cgaacagcgg
     7741 cgcttttttt ccctttgtcc gactgatttt ctgacgaatt ggccttcccg
//
