LOCUS       NZ_CP127253            13294 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Escherichia coli strain C41 plasmid pC4102, complete sequence.
ACCESSION   NZ_CP127253
VERSION     NZ_CP127253.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35637331
            Assembly: GCF_030285485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Bacteriocin-Producing Escherichia coli Q5 and Escherichia coli C41
            strains with probiotic properties: in silico, in vitro, in vivo
            studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13294)
  AUTHORS   Mihailovskaya,V., Sutormin,D., Trofimova,A., Mamontov,V.,
            Kuznetsova,M. and Severinov,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Laboratory of Metagenome Analysis, Skolkovo
            Institute of Science and Technology, Bolshoy Boulevard 30, bld. 1,
            Moscow 121205, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2023
            Assembly Method                   :: Flye v. 2.9.1
            Assembly Name                     :: E_coli_C41
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 16:37:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,051
            CDSs (total)                      :: 4,935
            Genes (coding)                    :: 4,732
            CDSs (with protein)               :: 4,732
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 119 of 203
            Pseudo Genes (incomplete)         :: 78 of 203
            Pseudo Genes (internal stop)      :: 45 of 203
            Pseudo Genes (multiple problems)  :: 37 of 203
            Pseudo Genes (short protein)      :: 2 of 203
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127253.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C41"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /culture_collection="VKM:B-3707D"
                     /db_xref="taxon:562"
                     /plasmid="pC4102"
                     /geo_loc_name="Russia: Perm krai"
                     /collection_date="2020-07"
                     /collected_by="Kuznetsova Marina, Mihailovskaya Veronika"
     gene            328..555
                     /locus_tag="QQ971_RS24595"
                     /old_locus_tag="QQ971_24605"
     CDS             328..555
                     /locus_tag="QQ971_RS24595"
                     /old_locus_tag="QQ971_24605"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(552..2120)
                     /gene="cea"
                     /locus_tag="QQ971_RS24600"
                     /old_locus_tag="QQ971_24610"
     CDS             complement(552..2120)
                     /gene="cea"
                     /locus_tag="QQ971_RS24600"
                     /old_locus_tag="QQ971_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2380..2817
                     /locus_tag="QQ971_RS24605"
                     /old_locus_tag="QQ971_24615"
     CDS             2380..2817
                     /locus_tag="QQ971_RS24605"
                     /old_locus_tag="QQ971_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2898..3317
                     /locus_tag="QQ971_RS24610"
                     /old_locus_tag="QQ971_24620"
     CDS             2898..3317
                     /locus_tag="QQ971_RS24610"
                     /old_locus_tag="QQ971_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3635..3868)
                     /locus_tag="QQ971_RS24615"
                     /old_locus_tag="QQ971_24625"
     CDS             complement(3635..3868)
                     /locus_tag="QQ971_RS24615"
                     /old_locus_tag="QQ971_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3875..4393)
                     /locus_tag="QQ971_RS24620"
                     /old_locus_tag="QQ971_24630"
     CDS             complement(3875..4393)
                     /locus_tag="QQ971_RS24620"
                     /old_locus_tag="QQ971_24630"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4431..4862)
                     /locus_tag="QQ971_RS24625"
                     /old_locus_tag="QQ971_24635"
                     /pseudo=""
     CDS             complement(<4431..4862)
                     /locus_tag="QQ971_RS24625"
                     /old_locus_tag="QQ971_24635"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5071..5394)
                     /locus_tag="QQ971_RS24630"
                     /old_locus_tag="QQ971_24640"
     CDS             complement(5071..5394)
                     /locus_tag="QQ971_RS24630"
                     /old_locus_tag="QQ971_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5458..5649
                     /locus_tag="QQ971_RS24635"
                     /old_locus_tag="QQ971_24645"
     CDS             5458..5649
                     /locus_tag="QQ971_RS24635"
                     /old_locus_tag="QQ971_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6676..6813)
                     /locus_tag="QQ971_RS24640"
                     /old_locus_tag="QQ971_24650"
     CDS             complement(6676..6813)
                     /locus_tag="QQ971_RS24640"
                     /old_locus_tag="QQ971_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            6975..7202
                     /locus_tag="QQ971_RS24645"
                     /old_locus_tag="QQ971_24655"
     CDS             6975..7202
                     /locus_tag="QQ971_RS24645"
                     /old_locus_tag="QQ971_24655"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(7199..8767)
                     /gene="cea"
                     /locus_tag="QQ971_RS24650"
                     /old_locus_tag="QQ971_24660"
     CDS             complement(7199..8767)
                     /gene="cea"
                     /locus_tag="QQ971_RS24650"
                     /old_locus_tag="QQ971_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            9027..9464
                     /locus_tag="QQ971_RS24655"
                     /old_locus_tag="QQ971_24665"
     CDS             9027..9464
                     /locus_tag="QQ971_RS24655"
                     /old_locus_tag="QQ971_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            9545..9964
                     /locus_tag="QQ971_RS24660"
                     /old_locus_tag="QQ971_24670"
     CDS             9545..9964
                     /locus_tag="QQ971_RS24660"
                     /old_locus_tag="QQ971_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(10282..10515)
                     /locus_tag="QQ971_RS24665"
                     /old_locus_tag="QQ971_24675"
     CDS             complement(10282..10515)
                     /locus_tag="QQ971_RS24665"
                     /old_locus_tag="QQ971_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(10522..11040)
                     /locus_tag="QQ971_RS24670"
                     /old_locus_tag="QQ971_24680"
     CDS             complement(10522..11040)
                     /locus_tag="QQ971_RS24670"
                     /old_locus_tag="QQ971_24680"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<11078..11509)
                     /locus_tag="QQ971_RS24675"
                     /old_locus_tag="QQ971_24685"
                     /pseudo=""
     CDS             complement(<11078..11509)
                     /locus_tag="QQ971_RS24675"
                     /old_locus_tag="QQ971_24685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(11718..12041)
                     /locus_tag="QQ971_RS24680"
                     /old_locus_tag="QQ971_24690"
     CDS             complement(11718..12041)
                     /locus_tag="QQ971_RS24680"
                     /old_locus_tag="QQ971_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            12105..12296
                     /locus_tag="QQ971_RS24685"
                     /old_locus_tag="QQ971_24695"
     CDS             12105..12296
                     /locus_tag="QQ971_RS24685"
                     /old_locus_tag="QQ971_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5735..5822)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(12382..12469)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5833..6391
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12480..13038
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3532..5081)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10179..11728)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc agtttagaag
       61 aggaggatgg tgcgatggtc cctccctgaa catcacgtat atagttagcc tgacatccaa
      121 caaggaggtt tatcgcgaat attcccacaa aaaatctttt cctcataact cgatccttat
      181 aaaatgaaaa gaatatatgg cgaggtttaa tttatgagct taagatacta cataaaaaat
      241 attttatttg gcctgtactg cacacttata tatatatacc ttataacaaa aaacagcgaa
      301 gggtattatt tccttgtgtc agataagatg ctatatgcaa tagtgataag cactattcta
      361 tgtccatatt caaaatatgc tattgaatac atagctttta acttcataaa gaaagatttt
      421 ttcgaaagaa gaaaaaacct aaataacgcc cccgtagcaa aattaaacct atttatgcta
      481 tataatctac tttgtttggt cctagcaatc ccatttggat tgctaggact ttttatatca
      541 ataaagaata attaaatccc taacacctca tttatagtat taagtttatt cttatcaata
      601 taggagcata gaattcctgt aacaatagca ataccccaaa tacctaatgt agttccagca
      661 agcaagctaa aaagtaaagc aacaacataa ctcacccctg catctgcagc tttcttctct
      721 aatgtaagaa atagtggctt ccagtcacct gtatccttaa tttttaggat atcagatacc
      781 acatcatatc caaaagaaac atgccccgta atcttcaagt acttggcaaa ctgatctaaa
      841 tgtttagccc agtcatcata cttcaccgat gccaacgcat taaaaatagc atcacgatcg
      901 gctttgctga atttcttatt taaaacatcc ttgtattttt caaaagcagc gagagcttca
      961 ttcacattgc cgattttctt acctttagac ttatcagcaa gttcctgtgc cattttcgaa
     1021 tatttttcac catatttttc agtcagcgtt tgataaaagc taactgttgc atcaacagca
     1081 tccttaatct gtgaattaag gagattattc tgtgcttttt tcaaattttc ttcagcttca
     1141 tgaacacgag cgataccggc attacgatta ttactgacct gagaaatagc cttctggatc
     1201 tgagttatat cagcatttat ccggttaata cgtgtttctg atgctgttac ctgtttttgt
     1261 ttttcttctc taatcttacc ggccccaacc cgtcgtctgg ttgcttcaaa aaaaggacgg
     1321 ttctgaagcg gatcattggc tcttggtgat agttttttga ccagctcatc cagttcttta
     1381 tatttagcgg atgcctgagc cagttcattt cgttttccag cgagcgtttt catttctgca
     1441 tcacgggcat ggatactgga gcttaaacga gaattgagag tcttaatctc tccatccatt
     1501 ttcaccactt cagattgtgc agcagaaagt tttttttggg cgatctcaac agctttagct
     1561 tcttcactca atgcagccag tcgtttctct tcagcttcag ccagtttcaa ctggcgttct
     1621 gtttcagcct tctcccgttc aatctcttta cgtcgttgtt ctgcttcctg aaaagccttt
     1681 tctgctgctt ccgcttcttt acgggctttt tcttctgctt tcgcaaggcg caaacgctct
     1741 gcttccgcct gcatagctgc attattagca tgagcaagct ctgttgctga aggcgtacgt
     1801 gaggcattgt gacgaagagc ctcattcacg atatccttca ggcgctgagt cagcgcatcc
     1861 ctgtttgcct ttgctttcgc ctgtgcttcc gctgcagctt ttgcccgggc agcctgctct
     1921 gcctgtgttt tctttaattg agcagtagac catttagcag ttgcatgaat agctgcagaa
     1981 ctttcacttt tactgcctcc ttttccacct ccgccgccag agccactccc gtcaggagta
     2041 ccattcaaaa gagtaataat tacctgtccc ttatcatcat aaggaacacc atctttatag
     2101 tacgctaccg cggtttccat tataaaatcc tctttgactt ttaaaacaat aagttaaaaa
     2161 taaatactgt acatataacc actggtttta tatacagcat aaaagctacg ccgctgcatt
     2221 ttccctgtca agactgtgga cttccatttt tgtgaaaacg atcaaaaaaa cagtctttca
     2281 caccacgcgc tattctcgcc cgatgccaca aaaaccagca caaacattac cgttctcaga
     2341 cctcattatg ttttactgaa actatgagat gagacatcta tgggacactg tcactttatg
     2401 gcatggcaca cactccggga cgcactaaaa atgacaggca gatcgcgttc acagttttac
     2461 cgtgatatgc gcgcaggcct tgtcagttac cgtaccggca gggacggacg acgggagttt
     2521 gaaaccagtg aactgatccg ggcatacggc gaattaaagc agaatgagac accagaaagg
     2581 cacagtgagg gacatgcaga aaatccacat gatcagcaga cagaacgcat tctccgggag
     2641 ctgaatgagc tgaaacaatg cctgacgctg atgcttgagg ataaacaggc acaggatatg
     2701 gatcgcagac gccaggaagc agaacgggaa cagctacaaa atgagatagc ccagctcagg
     2761 caggcactgg aactggaaaa gaaacgggga ttctggtcca ggttgttcgg tcgctgaacg
     2821 ctgtcagaga ctgatgataa aatagtcttc ggataataac tcaccgagaa taaatacttt
     2881 aaggtaggga gacactcatg agacgtaccg gaaacaaact ttgtcttatc gccatgataa
     2941 cagcaacagt agctctcaca gcctgtaccc caaagggcag cgtggaacaa catacccggc
     3001 attacgtata tgcttctgat gacggttttg atcccaactt ttccacccaa aaagccgaca
     3061 caacacgaat gatggtgcct ttttttcggc agttctggga tatgggagct aaagataaag
     3121 cgacaggaaa atcacggagt gatgtgcaac aacgcattca gcagtttcac agccaggaat
     3181 ttttaaactc actccggggc acaactcaat ttgcgggtac tgattaccgc agcaaagacc
     3241 ttaccccgaa aaaatccagg ctgctggctg acacgatttc tgcggtttat ctcgatggct
     3301 acgagggcag acagtaagtg gatttaccat aatcccttaa ttgtacgcac cgctaaaacg
     3361 cgttcagcgc gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg
     3421 ccgcgatcgc gcctgataaa ttttaaccgt atgaatacct atgcaaccag agggtacagg
     3481 ccacattacc cccacttaat ccactgaagc tgccattttt catggtttca ccatcccagc
     3541 gaagggccat ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac
     3601 acatgttcgc gctcctgtat cagccgttcc tctcttatct ccagttctcg ctgtataact
     3661 ggctcaagcg ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc
     3721 gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa catcttctgc aattccacga
     3781 aggcgctctc ccattcgctc agccgctgca tatagtcctg ttgcagctgc tctaaggcgt
     3841 tcagcaaatg tgtttccagc tctgtcactc tgtgtcactc cttcagatgt acccactctt
     3901 tcccctgaaa gggaatcacc tccgctgatt tcccgtacgg aaggacaagg aatttcctgt
     3961 tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt
     4021 cctgcttcct gatttcttcc aggttcgcct gtatcctccc tccaagatac cagagcgtcc
     4081 cgccactcgc ggtaaacagg agaaagacta tccccagtaa catcatgccc gtattccctg
     4141 ccagctttaa cacgtccctc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt
     4201 tttccagcga ttcctctgtc gaggctgtga acagggctat agcgtctctg attttcgtct
     4261 cgtttgatgt cacagcctcg cttacagatt cgccgagcct cctgaactcg ttgttcagca
     4321 ttttctctgt agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgca
     4381 aaagattgct cataaaatgc tcctttcagc ctgatattct tcccgccgtt cggatctgca
     4441 atgctgatac tgcttcgcgt caccctgacc acttccagcc ccgcctcagt gagcgcctga
     4501 atcacatcct gacggccttt tatctctccg gcatggtaaa gtgcatctat acctcgcgtg
     4561 acgccctcag caagcgcctg tttcgtttca ggcaggttat cagggagtgt cagcgtcctg
     4621 cggttctccg gggcgttcgg gtcatgcagc ccgtaatggt gattcaccag cgtctgccag
     4681 gcatcaattc taggcctgtc tgcgcggtcg tagtacggct ggaggcgttt tccggtctgt
     4741 agctccatgt tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc
     4801 cacaggatgc tgtactgatt tttttcgaga ccgggcatca gtacacgctc aaagctcgcc
     4861 atcacttttt cacgtcctcc cggcggcagc tccttctccg cgaacgacag aacaccggac
     4921 gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc ggacttcttc cggattaccc
     4981 tgaagcaccg ttgcgccttc gcggttacgc tccctcccca gcaggtaatc aaccggacca
     5041 ctgccaccac cttttcccct ggcatgaaat ttaactatca tcccgcgccc cctgttccct
     5101 gacagccaga cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg
     5161 aacccggtca ccggaagacc actgcccgct gttcacctta cgggctgtct gattcaggtt
     5221 atttccgatg gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gttttccgga
     5281 acgggcaacc ggctccccca ggcagacccg ccgcatccat accgccagtt gtttaccctc
     5341 acagcgttca agtaaccggg catgttcatc atcagtaacc cgtattgtga gcatcctctc
     5401 gcgtttcatc ggtatcatta ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     5461 actaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     5521 ctgctggaga agctcaacga actggacgca gatgaacagg ccgatatttg tgaatcgctt
     5581 cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac gtttcgggga tgacggtgaa
     5641 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtgagc ggatgccggg
     5701 agctgacaag cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg cgcagccctg
     5761 acccagtcac gtagcgatag cggagtgtat actggcttaa ccatgcggca tcagtgcgga
     5821 ttgtatgaaa agtacgccat gccgggtgtg aaatgccgca cagatgcgta aggagaaaat
     5881 gcacgtccag gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     5941 tgcggcgagc ggtactgact cacacaaaaa cggtaacaca gttatccaca gaatcagggg
     6001 ataaggccgg aaagaacatg tgagcaaaag accaggaaca ggaagaaggc cacgtagcag
     6061 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6241 ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt
     6301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6361 ggtaactatc gtcttgagtc caacccggta aggcacgcct taacgccact ggcagcagcc
     6421 actggtaacc ggattagcag agcgatgatg gcacaaacgg tgctacagag ttcttgaagt
     6481 agtggcccga ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6541 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgttggta
     6601 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6661 atcctttgat cttttctact gaaccgcgat ccccgtcagt ttagaagagg aggatggtgc
     6721 gatggtccct ccctgaacat cacgtatata gttagcctga catccaacaa ggaggtttat
     6781 cgcgaatatt cccacaaaaa atcttttcct cataactcaa tccttataaa atgaaaagaa
     6841 tatatggcga ggtttaattt atgagcttaa gatactacat aaaaaatatt ttatttggcc
     6901 tgtactgcac acttatatat atatacctta taacaaaaaa cagcgaaggg tattatttcc
     6961 ttgtgtcaga taagatgcta tatgcaatag tgataagcac tattctatgt ccatattcaa
     7021 aatatgctat tgaatacata gcttttaact tcataaagaa agattttttc gaaagaagaa
     7081 aaaacctaaa taacgccccc gtagcaaaat taaacctatt tatgctatat aatctacttt
     7141 gtttggtcct agcaatccca tttggattgc taggactttt tatatcaata aagaataatt
     7201 aaatccctaa cacctcattt atagtattaa gtttattctt atcaatatag gagcatagaa
     7261 ttcctgtaac aatagcaata ccccaaatac ctaatgtagt tccagcaagc aagctaaaaa
     7321 gtaaagcaac aacataactc acccctgcat ctgcagcttt cttctctaat gtaagaaata
     7381 gtggcttcca gtcacctgta tccttaattt ttaggatatc agataccaca tcatatccaa
     7441 aagaaacatg ccccgtaatc ttcaagtact tggcaaactg atctaaatgt ttagcccagt
     7501 catcatactt caccgatgcc aacgcattaa aaatagcatc acgatcggct ttgctgaatt
     7561 tcttatttaa aacatccttg tatttttcaa aagcagcgag agcttcattc acattgccga
     7621 ttttcttacc tttagactta tcagcaagtt cctgtgccat tttcgaatat ttttcaccat
     7681 atttttcagt cagcgtttga taaaagctaa ctgttgcatc aacagcatcc ttaatctgtg
     7741 aattaaggag attattctgt gcttttttca aattttcttc agcttcatga acacgagcga
     7801 taccggcatt acgattatta ctgacctgag aaatagcctt ctggatctga gttatatcag
     7861 catttatccg gttaatacgt gtttctgatg ctgttacctg tttttgtttt tcttctctaa
     7921 tcttaccggc cccaacccgt cgtctggttg cttcaaaaaa aggacggttc tgaagcggat
     7981 cattggctct tggtgatagt tttttgacca gctcatccag ttctttatat ttagcggatg
     8041 cctgagccag ttcatttcgt tttccagcga gcgttttcat ttctgcatca cgggcatgga
     8101 tactggagct taaacgagaa ttgagagtct taatctctcc atccattttc accacttcag
     8161 attgtgcagc agaaagtttt ttttgggcga tctcaacagc tttagcttct tcactcaatg
     8221 cagccagtcg tttctcttca gcttcagcca gtttcaactg gcgttctgtt tcagccttct
     8281 cccgttcaat ctctttacgt cgttgttctg cttcctgaaa agccttttct gctgcttccg
     8341 cttctttacg ggctttttct tctgctttcg caaggcgcaa acgctctgct tccgcctgca
     8401 tagctgcatt attagcatga gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac
     8461 gaagagcctc attcacgata tccttcaggc gctgagtcag cgcatccctg tttgcctttg
     8521 ctttcgcctg tgcttccgct gcagcttttg cccgggcagc ctgctctgcc tgtgttttct
     8581 ttaattgagc agtagaccat ttagcagttg catgaatagc tgcagaactt tcacttttac
     8641 tgcctccttt tccacctccg ccgccagagc cactcccgtc aggagtacca ttcaaaagag
     8701 taataattac ctgtccctta tcatcataag gaacaccatc tttatagtac gctaccgcgg
     8761 tttccattat aaaatcctct ttgactttta aaacaataag ttaaaaataa atactgtaca
     8821 tataaccact ggttttatat acagcataaa agctacgccg ctgcattttc cctgtcaaga
     8881 ctgtggactt ccatttttgt gaaaacgatc aaaaaaacag tctttcacac cacgcgctat
     8941 tctcgcccga tgccacaaaa accagcacaa acattaccgt tctcagacct cattatgttt
     9001 tactgaaact atgagatgag acatctatgg gacactgtca ctttatggca tggcacacac
     9061 tccgggacgc actaaaaatg acaggcagat cgcgttcaca gttttaccgt gatatgcgcg
     9121 caggccttgt cagttaccgt accggcaggg acggacgacg ggagtttgaa accagtgaac
     9181 tgatccgggc atacggcgaa ttaaagcaga atgagacacc agaaaggcac agtgagggac
     9241 atgcagaaaa tccacatgat cagcagacag aacgcattct ccgggagctg aatgagctga
     9301 aacaatgcct gacgctgatg cttgaggata aacaggcaca ggatatggat cgcagacgcc
     9361 aggaagcaga acgggaacag ctacaaaatg agatagccca gctcaggcag gcactggaac
     9421 tggaaaagaa acggggattc tggtccaggt tgttcggtcg ctgaacgctg tcagagactg
     9481 atgataaaat agtcttcgga taataactca ccgagaataa atactttaag gtagggagac
     9541 actcatgaga cgtaccggaa acaaactttg tcttatcgcc atgataacag caacagtagc
     9601 tctcacagcc tgtaccccaa agggcagcgt ggaacaacat acccggcatt acgtatatgc
     9661 ttctgatgac ggttttgatc ccaacttttc cacccaaaaa gccgacacaa cacgaatgat
     9721 ggtgcctttt tttcggcagt tctgggatat gggagctaaa gataaagcga caggaaaatc
     9781 acggagtgat gtgcaacaac gcattcagca gtttcacagc caggaatttt taaactcact
     9841 ccggggcaca actcaatttg cgggtactga ttaccgcagc aaagacctta ccccgaaaaa
     9901 atccaggctg ctggctgaca cgatttctgc ggtttatctc gatggctacg agggcagaca
     9961 gtaagtggat ttaccataat cccttaattg tacgcaccgc taaaacgcgt tcagcgcgat
    10021 cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcgcc
    10081 tgataaattt taaccgtatg aatacctatg caaccagagg gtacaggcca cattaccccc
    10141 acttaatcca ctgaagctgc catttttcat ggtttcacca tcccagcgaa gggccatcca
    10201 gcgtgcgttc ctgtatttcc ggctgacgct cccgttccag ggataacaca tgttcgcgct
    10261 cctgtatcag ccgttcctct cttatctcca gttctcgctg tataactggc tcaagcgttc
    10321 tgtctgctcg ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat
    10381 cgcgttgtcc cgttgcgtaa gcgaaaacat cttctgcaat tccacgaagg cgctctccca
    10441 ttcgctcagc cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt
    10501 ttccagctct gtcactctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg
    10561 aatcacctcc gctgatttcc cgtacggaag gacaaggaat ttcctgttcc cgtcctgcac
    10621 aaactccacg ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat
    10681 ttcttccagg ttcgcctgta tcctccctcc aagataccag agcgtcccgc cactcgcggt
    10741 aaacaggaga aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac
    10801 gtccctcctg tgctgcatca tcgcctcttt caccccttcc cggtgttttt ccagcgattc
    10861 ctctgtcgag gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac
    10921 agcctcgctt acagattcgc cgagcctcct gaactcgttg ttcagcattt tctctgtaga
    10981 ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgcaaaa gattgctcat
    11041 aaaatgctcc tttcagcctg atattcttcc cgccgttcgg atctgcaatg ctgatactgc
    11101 ttcgcgtcac cctgaccact tccagccccg cctcagtgag cgcctgaatc acatcctgac
    11161 ggccttttat ctctccggca tggtaaagtg catctatacc tcgcgtgacg ccctcagcaa
    11221 gcgcctgttt cgtttcaggc aggttatcag ggagtgtcag cgtcctgcgg ttctccgggg
    11281 cgttcgggtc atgcagcccg taatggtgat tcaccagcgt ctgccaggca tcaattctag
    11341 gcctgtctgc gcggtcgtag tacggctgga ggcgttttcc ggtctgtagc tccatgttcg
    11401 gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt
    11461 actgattttt ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc actttttcac
    11521 gtcctcccgg cggcagctcc ttctccgcga acgacagaac accggacgtg tatttcttcg
    11581 caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg
    11641 cgccttcgcg gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt
    11701 ttcccctggc atgaaattta actatcatcc cgcgccccct gttccctgac agccagacgc
    11761 agccggcgca gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcaccg
    11821 gaagaccact gcccgctgtt caccttacgg gctgtctgat tcaggttatt tccgatggcg
    11881 gccagctgac gcagtaacgg cggtgccagt gtcggcagtt ttccggaacg ggcaaccggc
    11941 tcccccaggc agacccgccg catccatacc gccagttgtt taccctcaca gcgttcaagt
    12001 aaccgggcat gttcatcatc agtaacccgt attgtgagca tcctctcgcg tttcatcggt
    12061 atcattaccc catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa
    12121 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctg ctggagaagc
    12181 tcaacgaact ggacgcagat gaacaggccg atatttgtga atcgcttcac gaccacgccg
    12241 atgagcttta ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca
    12301 tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc tgacaagccc
    12361 gtcagggcgc gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta
    12421 gcgatagcgg agtgtatact ggcttaacca tgcggcatca gtgcggattg tatgaaaagt
    12481 acgccatgcc gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgca cgtccaggcg
    12541 cttttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt
    12601 actgactcac acaaaaacgg taacacagtt atccacagaa tcaggggata aggccggaaa
    12661 gaacatgtga gcaaaagacc aggaacagga agaaggccac gtagcaggcg tttttccata
    12721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    12781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    12841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    12901 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    12961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    13021 ttgagtccaa cccggtaagg cacgccttaa cgccactggc agcagccact ggtaaccgga
    13081 ttagcagagc gatgatggca caaacggtgc tacagagttc ttgaagtagt ggcccgacta
    13141 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    13201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gttggtagcg gtggtttttt
    13261 tgtttgcaag cagcagatta cgcgcagaaa aaaa
//
