LOCUS       NZ_CP127238            10060 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Klebsiella pneumoniae strain 130128 plasmid p2_130128, complete
            sequence.
ACCESSION   NZ_CP127238
VERSION     NZ_CP127238.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35632517
            Assembly: GCF_030284485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Gong,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 130128
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Gong,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 18:41:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,843
            CDSs (total)                      :: 5,720
            Genes (coding)                    :: 5,563
            CDSs (with protein)               :: 5,563
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 57 of 157
            Pseudo Genes (incomplete)         :: 105 of 157
            Pseudo Genes (internal stop)      :: 17 of 157
            Pseudo Genes (multiple problems)  :: 22 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127238.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130128"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_130128"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018-06-28"
     gene            complement(join(9992..10060,1..303))
                     /gene="tolA"
                     /locus_tag="QRA17_RS29100"
                     /old_locus_tag="QRA17_29100"
     CDS             complement(join(9992..10060,1..303))
                     /gene="tolA"
                     /locus_tag="QRA17_RS29100"
                     /old_locus_tag="QRA17_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(577..822)
                     /locus_tag="QRA17_RS29105"
                     /old_locus_tag="QRA17_29105"
     CDS             complement(577..822)
                     /locus_tag="QRA17_RS29105"
                     /old_locus_tag="QRA17_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1149..2834
                     /locus_tag="QRA17_RS29110"
                     /old_locus_tag="QRA17_29110"
     CDS             1149..2834
                     /locus_tag="QRA17_RS29110"
                     /old_locus_tag="QRA17_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2844..3101
                     /locus_tag="QRA17_RS29115"
                     /old_locus_tag="QRA17_29115"
     CDS             2844..3101
                     /locus_tag="QRA17_RS29115"
                     /old_locus_tag="QRA17_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3186..3335
                     /locus_tag="QRA17_RS29120"
                     /old_locus_tag="QRA17_29120"
     CDS             3186..3335
                     /locus_tag="QRA17_RS29120"
                     /old_locus_tag="QRA17_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4819..6783
                     /locus_tag="QRA17_RS29125"
                     /old_locus_tag="QRA17_29125"
     CDS             4819..6783
                     /locus_tag="QRA17_RS29125"
                     /old_locus_tag="QRA17_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6783..7514
                     /gene="mobC"
                     /locus_tag="QRA17_RS29130"
                     /old_locus_tag="QRA17_29130"
     CDS             6783..7514
                     /gene="mobC"
                     /locus_tag="QRA17_RS29130"
                     /old_locus_tag="QRA17_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7521..8051
                     /locus_tag="QRA17_RS29135"
                     /old_locus_tag="QRA17_29135"
     CDS             7521..8051
                     /locus_tag="QRA17_RS29135"
                     /old_locus_tag="QRA17_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8078..8257)
                     /locus_tag="QRA17_RS29140"
                     /old_locus_tag="QRA17_29140"
     CDS             complement(8078..8257)
                     /locus_tag="QRA17_RS29140"
                     /old_locus_tag="QRA17_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8283..8711)
                     /locus_tag="QRA17_RS29145"
                     /old_locus_tag="QRA17_29145"
     CDS             complement(8283..8711)
                     /locus_tag="QRA17_RS29145"
                     /old_locus_tag="QRA17_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8875..9189)
                     /locus_tag="QRA17_RS29150"
                     /old_locus_tag="QRA17_29150"
     CDS             complement(8875..9189)
                     /locus_tag="QRA17_RS29150"
                     /old_locus_tag="QRA17_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9177..9455)
                     /locus_tag="QRA17_RS29155"
                     /old_locus_tag="QRA17_29155"
     CDS             complement(9177..9455)
                     /locus_tag="QRA17_RS29155"
                     /old_locus_tag="QRA17_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9630..9995)
                     /locus_tag="QRA17_RS29160"
                     /old_locus_tag="QRA17_29160"
     CDS             complement(9630..9995)
                     /locus_tag="QRA17_RS29160"
                     /old_locus_tag="QRA17_29160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1..7834
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3439..4017)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6784..7511
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tttggcgtgg gtaacggctg atattgctgc tttacagagt ttcggatctc ctccttcagc
       61 aatggcgctg ttcagagtgc catcacgttg tatggatatc cgcacggtgc acatttttcc
      121 cgcataggcc tctggttcaa acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta
      181 gctactgatt ttagatgact tatccagagt ataaattttc ggcggctggg tggtggaatg
      241 cttacaggcc gtcaggagta aggggctgca caatgccagc aggataaagg gtattaaacg
      301 catcgggttg atgacctgtc agtaaaaaat gttacatgta tatttgtcac tctttacggc
      361 cacgttcatt tacataaaca cggcttatcg tatcgttttt acaccaaacg gcccgccatg
      421 cagttgcgtg cataggcatg cagcgggtta caatttaccg ggcgcgtttc cggcggtttt
      481 ccgggtggtt tgttgcctgt tttatcccgt agccgccgga aacgccctca gaccgtctga
      541 gcggtgcggg caatgcgacg ttatggtaaa taccccttac cggcggcgct gtggctgcca
      601 ggtgaaggtg gtttccgtag cctcgtattt ctcccgacgc atttcctgcg cccgcctggc
      661 ttcacgttcg tcctgaatgg cacgaagcac cctttcccgc acctgttgtt ttaccgcctt
      721 tggcagcgtt ttgttccctt tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc
      781 caggcgcttt tcttcagcgg gagtcagttt caggtgcgtc atagtggcct ccgggcgtct
      841 caggtttccc gaacagttta accgcaggtc ctctcctcat gccagttagc tttcagatgt
      901 gtctatacca tctgaaatgt aagtgtgatg aatgtcttaa ataataaaat ttgttgccat
      961 gagaaatttt cgggaaaaga cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc
     1021 ctgaagatga gaactaatct catatattga cacctgaaaa cgggaggagt aaggtaataa
     1081 ttatactgtg tatatgtaca gtatgttggc tttaatttat tgttttaaat gtcaaagagg
     1141 aaaacggtat gagtggtgga gacggtcgag gtccgggtaa ttcaggtctg ggacataatg
     1201 gtggtcaggc cagtgggaat gtgaacggta cgtctggtaa aggtggccct tcatcaggtg
     1261 gtggtacgga tccaaacagc gggccgggct ggggtacgac gcatacgccg aatggagata
     1321 ttcataacta taatccgggg gagtttggta acggcgggag taaacccggt ggtaatggtg
     1381 gtaacagcgg caatcatagc ggtagctctg gtggcggaca gtcttcggcc actgcgatgg
     1441 ccttcggtct gcctgccctg gctactccgg gggctgaagg actggcttta tcagtttccg
     1501 gcgatgcgtt gtcggccgcc gttgctgatg tgctggctgc cctgaagggg ccgtttaagt
     1561 ttggtctgtg ggggattgcg atctacggtg tgctgccttc tgagattgca aaagatgatc
     1621 cgaaaatgat gtcaaaaatt atgacgtcat taccggccga tgcggtgacg gagactccgg
     1681 caagtacttt accactggac caggcgacgg ttcgtgtcag acaacgggtt gtggatgtgg
     1741 tgaaggatga gcggcagcat attgcggttg tcgcaggtcg gccaatgagt gtccctgtgg
     1801 tggatgcgaa accgacaaaa cgtccggggg tattcagtgt gtcgattccg ggtctcccgt
     1861 ctctgcaggt gagcgtaccg aaaggtgttc cgacagcgaa agccccgcca aaaggcattg
     1921 ttgctgaaaa aggtgattca cgtccggctg gttttacagc cggtggtaac tcccgtgagg
     1981 ccgttattcg tttcccgaaa gagaccggac agaagccggt ttatgtgtcg gtgacagatg
     2041 ttcttacccc ggcacaggta aaacagcgtc aggaggaaga aaagcgtcgc cagcaggcat
     2101 gggacgccgc tcatccggaa gaggggctga aaagagacta tgataaagcg aaagctgagc
     2161 tggatgccga agataaaaat attgcgacct taaacagccg cattgcatcg acagagaagg
     2221 cgctccccgg tgcaagggct gctgttcagg aagccgataa aaaggtaaaa gaggcagagg
     2281 caaataaaga tgattttgtg acttataacc cgcctcatga atatggctcc gggtggcagg
     2341 atcaggttcg ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagcggctc
     2401 aggcatcttt aaacgcaatg aatgaatcct tatccagaga taaggctgca cttaccgggg
     2461 cgatggagag ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aagttaaatg
     2521 aggaaaagaa caagcctcgc aaggggacta aagactacgg ccatgattat catccagtac
     2581 ctaaaacaga agacataaag ggacttggag agttaaagcg aggtgatcct aaaacaccta
     2641 aacaaggtgg tggtggtaag cgggcccgat ggtatggcga taaaaaacgt aaaatttatg
     2701 aatgggattc ccagcacggt gagcttgaag ggtatcgtgc cagtgatggc gaacacctag
     2761 gcgcatttga tcccaaaacg ggtaagcagg ttaaagggcc agatccaaaa cggaacatta
     2821 aaaaatatct ttgagaggta aatatggggc ttaaattacg tttgacatgg tttgataaga
     2881 aaactgaaga gtttaaaggg gaagagtatt ctaaagactt tggtgacgat ggttctgtca
     2941 ttgaaagctt gggaatgcct ttaaaggata atattaataa tggtgagttt gatttgaaga
     3001 aagaatgggt tcctttacta cagccatttt ttaaaaatca aataaaacct gatgtggata
     3061 attgttatat agcttttgat tatcgggacg gtaactggta acaaaaaatt atcaaggatg
     3121 atttgaaata ctgacttccg gtgttcaggg atgaatgccg gagtctttaa atacagggaa
     3181 agtatatgaa aaaggtaaag actatttttc tttttatatt gattgttgct ggctttcttc
     3241 tggccgcatg tcaggcaaat tatatccggg atgttcaggg cggaacggtg gcaccatcgt
     3301 cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga
     3361 tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg
     3421 tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga
     3481 gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca
     3541 accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag
     3601 tggcgttttg cgtgctttcc cgggttggac tcaagtgaac agttaccgga taaggcgcag
     3661 cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc
     3721 gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca agggagaacg
     3781 gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgacct
     3841 accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg
     3901 gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc
     3961 aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg
     4021 ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg cagcgcccgc
     4081 cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat ttccccggca
     4141 cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt aagtctttct
     4201 attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc tgaacatttt
     4261 acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc atccccgctg
     4321 gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc caccgacgca
     4381 cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc agaaattcac
     4441 ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct ggacgcacct
     4501 gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact
     4561 ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac
     4621 gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg cggaaagagg
     4681 ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg ccgccatgcc
     4741 ttggggcatg tccgcggctt gtgcagcggg ggctgcgcat cacgccggta aacccgtcgt
     4801 ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg gcatatcccg
     4861 gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta ttacagtctg
     4921 gccggttttt ttctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggccga
     4981 atgcgttttg tggggctgag ccataaggcg cggcgggagt atgccgatcg ctataagcgc
     5041 gataataacc ggactgtgcc attttcggtg ctggcctcgc tggattcacc cgggcggatg
     5101 gtcagggcac ggcgacatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg
     5161 gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg
     5221 cgcccggacc tctatgcgaa cgcagattat tacctgctgc agccggctaa cctgttcggg
     5281 atagcctggt gtgccggagg gcttgtcgcc ggcgtccttc tggcgcggct gctggcgttc
     5341 ctgctgcttg atggtcattt tgcggccgct gacgaggcgg tgaacgcgaa actgaatcag
     5401 gagagccggc gcagcagcca gcggaccgga gaaatgacag acgtccgcca cctgcatttc
     5461 ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc ccggaaacag
     5521 caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc tcgtgatacc
     5581 tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag cgcgatggcc
     5641 accaacgcgc tcattcgctg cgtacgcgat ttcggggacg ctgtggtgta cttcgacccc
     5701 aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt caccctgctg
     5761 gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga ccagtacgcc
     5821 ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac
     5881 taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc gcagggggca
     5941 aacattcagc aggtgctggc ggcggcgtac gcgttgccgg aggccctgaa aaaggacgtg
     6001 aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca gacggacagc
     6061 ggtatcgatg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg
     6121 gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc
     6181 attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga tgagattaaa
     6241 tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg ggactgcaac
     6301 ctgctgctcg cgcaccagtc gctgggcgac ttcggtcagt gcgggcagga tctgcccgct
     6361 gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta tcgcgcggcc
     6421 agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg agatccgggt ggacgtggag
     6481 cgccgccggg ccagccggga ggcggggaac gtggagcaca tcagcggcga cagcttcatc
     6541 cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc tgacgggttc
     6601 gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct gcgggttgag
     6661 cgccgggaaa tttcgctgaa aaccttcccg gtgctggcga aaacggaccc gctggcggag
     6721 tacctgcctc cggccgacgg cggcggacca aaagcggatg atgattttgc ggggctttac
     6781 tgatgacact ggagcagtac aacgtcagcc acgcgaagcg gcaggcgcgg aacgcggaga
     6841 aaacacggct gaccctgcgc tggctgcggg aagagctgtg ttccacggcg gagctggttg
     6901 cgcgacggct ggggattgcg gcggtacagc cggtttaccg cttcctggac agtctggtgg
     6961 cgaaagggct gctggtccgg gcgaaatacc cggttgacgg ccgtcaggtc agcgtgtggg
     7021 ggctgacgcc gcacggggtg gcgttctcgt tcgacgagga cgagccgctg acggacgtta
     7081 ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc gcaccggctg gccgtgcagt
     7141 ccctgcgcct ctccatggag ggccgggggg ccaccggctg gcggtacctg caccgtatgg
     7201 ccctgaaagg gatgaaagtg ccggacgcgc tggcggagct ggacggcaga acggtggcat
     7261 ttgaggtaga gcgcaccgtc aagagccgca ggcggtatca ggaagtggtg tccgggtatc
     7321 tgttcaaccg ccgggcgaac ggcatcgatg aaatctggta catctgccct gaccggccga
     7381 cgcttgtccg ggtacagcgg gcgattctgg cggtggatga aatcgtgaat ccacagaccg
     7441 gcgaggcccg caaaacggtg gagctggacc gggagaggct gttcgcgtgc tttaaattta
     7501 tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaggac gtggaagaca
     7561 aagacggcga gcgctgcatt atgccaacca gcgagcatct ggaagacatg gtggtgctcg
     7621 cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga
     7681 tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc acgtactttt
     7741 tcaccggtga aaatccgcaa tgggaacggg acaaacccac aggaatgaag ctgtgtgatt
     7801 acctctatcg aaaggtggag cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg
     7861 ggaagtgctc accggagcag attaacgcga tgattgaggt ggtcagcgag cggcacgggg
     7921 aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac agccgctatt
     7981 ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg tggccgttcg
     8041 gcaaaaaata acgccagcac cggcatggcc ggtgctgtta ttcttccccg agcttatccc
     8101 gcatcacctg ccagagtgtt tccgcatcct cgtgaagccg ttcgcacatg tcggccgggg
     8161 tatccatgtc cagctcgttc agcttttcca gcaacagcag cgactgatcc ttaatgaatt
     8221 tagccatatt gagggcagtt ttttcctgct tcgtcatgtt ctccattccc ttcggtaagt
     8281 tctcagctaa accagccgga ccagaagcgt tttttcttct gtgtcagttc tgttgtcaat
     8341 ctgtccactt cggcctggag tttccggcgc tcatcatcgt gctggcggcg ttcttcttcc
     8401 cgggatgttt tatcttccag catcagggtg acagcctgcc ggagatcggc cagttccttt
     8461 tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg tattttcacg gtgtgaaatg
     8521 ccgggaattt gtttctcagg gatattcaca tttccgtata cacggatcag ttctgacaca
     8581 tccacgacag ggttgttttt cccgtcacgt gacacggtaa cctttccctg ttttacatga
     8641 ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg cctgtgaaag gttgagcaat
     8701 gttttcgcca ctgtataccc ctgattttat cgggtgtgaa aggtgtgtaa tttctcacgg
     8761 taaatgtatc gctgaatttt acccgttttt ccgttcagga tgtggttttc tgacgcgctg
     8821 cgcttgtcaa accggcgacg gccacgcaat gaattgcgcc cccgtcatcg gttattagag
     8881 gctgtctgcc cccggatacc cggattcttt ctgatgcctg acggtgacga tcacaatctg
     8941 gtccatttcc cggtcgtggc ggtagagcat cacatagcca ctgtcgccaa acccgatcac
     9001 cagttcctta tattccagcg gcagaaacgg caccgggcgg ccaatgtctg gcaatgtttt
     9061 cagttgctgg atagcccgga cgatcacctc acctcctttt ctggctgcca gccgattttt
     9121 ggttttgaga aagtcctgaa ggcgttgtaa atcgcgctgt gccagcgcgg aaatcgttac
     9181 ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc aggtacttat ccaggcttcc
     9241 gcttcttccg cagtaaggtg cagacccgtt tcctgatagt gctgccatgc ggcctgtccg
     9301 tcccgcaggt actggctgcg cttctcttcc cggtcgatgt attcggtgat tgcctccagc
     9361 attagcgcgt gcgctgagcg gtgccggtca tcggccagcg ttttcagccg gtccttaagt
     9421 tcttcatcga gcctgatgga tgttgctgat gacataaaag cccctttgta gtcatatgta
     9481 ttacacatga ctactttaat ccgtggcggc gctatttgcc agaaatacgg cttttgctgc
     9541 ccatttttca gcttcgcttc cactaatcag ggctgctaca ggctcactca tcatccagga
     9601 ctattgcagc ctgcgggtta aaggacctgt tactggcggc aaccatcatc attgagcttt
     9661 tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt tcagactgaa ttcaaaattt
     9721 ataacctgtc gcattgccgg gtgccctgcg ggatatagcc agtgcctcat tgccgtcagc
     9781 gtttcccgaa aaaaaaggtg acagccggta agcgacacgt tgtccactcg tccatcgtcc
     9841 agaatatcaa acgtggcggt cacctgacct tcgatgcgtt ttgctgcggc ctgtaccggg
     9901 taggttggct gcagtgtggt tacgggtttt agtggtatcg catgtaccgg agtgaggaac
     9961 atggccgcca gcactgtcag agtaaaagct ttcatggtct gaaatccaga ggggcgtttt
    10021 tgaatatttg ccaggtattt tcatccggtg ccggcggaat
//
