LOCUS       NZ_CP127234             5596 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Klebsiella pneumoniae strain 130119 plasmid p3_130119, complete
            sequence.
ACCESSION   NZ_CP127234
VERSION     NZ_CP127234.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35631498
            Assembly: GCF_030284505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Gong,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 130119
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Gong,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 18:44:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,884
            CDSs (total)                      :: 5,762
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 68 of 180
            Pseudo Genes (incomplete)         :: 121 of 180
            Pseudo Genes (internal stop)      :: 33 of 180
            Pseudo Genes (multiple problems)  :: 34 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127234.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130119"
                     /isolation_source="tracheal secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3_130119"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-03-09"
     gene            complement(329..457)
                     /locus_tag="QRA14_RS29370"
                     /old_locus_tag="QRA14_29375"
     CDS             complement(329..457)
                     /locus_tag="QRA14_RS29370"
                     /old_locus_tag="QRA14_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            620..844
                     /locus_tag="QRA14_RS29375"
                     /old_locus_tag="QRA14_29380"
     CDS             620..844
                     /locus_tag="QRA14_RS29375"
                     /old_locus_tag="QRA14_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            828..1094
                     /locus_tag="QRA14_RS29380"
                     /old_locus_tag="QRA14_29385"
     CDS             828..1094
                     /locus_tag="QRA14_RS29380"
                     /old_locus_tag="QRA14_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1164..1514)
                     /locus_tag="QRA14_RS29385"
                     /old_locus_tag="QRA14_29390"
     CDS             complement(1164..1514)
                     /locus_tag="QRA14_RS29385"
                     /old_locus_tag="QRA14_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1580..2125)
                     /locus_tag="QRA14_RS29390"
                     /old_locus_tag="QRA14_29395"
     CDS             complement(1580..2125)
                     /locus_tag="QRA14_RS29390"
                     /old_locus_tag="QRA14_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2151..2477)
                     /locus_tag="QRA14_RS29395"
                     /old_locus_tag="QRA14_29400"
     CDS             complement(2151..2477)
                     /locus_tag="QRA14_RS29395"
                     /old_locus_tag="QRA14_29400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3633..3809
                     /locus_tag="QRA14_RS29400"
                     /old_locus_tag="QRA14_29405"
     CDS             3633..3809
                     /locus_tag="QRA14_RS29400"
                     /old_locus_tag="QRA14_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3799..4143
                     /locus_tag="QRA14_RS29405"
                     /old_locus_tag="QRA14_29410"
     CDS             3799..4143
                     /locus_tag="QRA14_RS29405"
                     /old_locus_tag="QRA14_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4352..>4786
                     /locus_tag="QRA14_RS29410"
                     /old_locus_tag="QRA14_29415"
                     /pseudo=""
     CDS             4352..>4786
                     /locus_tag="QRA14_RS29410"
                     /old_locus_tag="QRA14_29415"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4821..5330
                     /locus_tag="QRA14_RS29415"
                     /old_locus_tag="QRA14_29420"
     CDS             4821..5330
                     /locus_tag="QRA14_RS29415"
                     /old_locus_tag="QRA14_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5337..5549
                     /locus_tag="QRA14_RS29420"
                     /old_locus_tag="QRA14_29425"
     CDS             5337..5549
                     /locus_tag="QRA14_RS29420"
                     /old_locus_tag="QRA14_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3468..3547
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2885..3282)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4134..5596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.795"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 cggcagcacg aaaaggaact gacggaagcg cgttcccggc agcgaagcta tgacggtccg
       61 tcgctgggct gaaatgcagg aaaacagggt tctcatcggg catttacgct gaaaccggat
      121 gtcattcacc tggctgcatg gctatgcagc caggtaaaat ttatctgtcg cgaacggggc
      181 ggtttatcgg gtggtttgtt gccggtttta cctgactgcc accccgttcg cggcgaaccc
      241 gtccggggcg gtgcgggcaa cgggtgttat gtttaatcag cgtgaacggc agatacaaaa
      301 aagccccgga gggcttttaa ctactgactt atttttttga agatttgctg ggtttcaatt
      361 tgtcagtatt tcccggcgtt tttttattgt cacgctggcg tttatcaggt tcccctgttg
      421 aatcggcaat tcgtttcgga cccggcttaa gacccataat cgttatcctt tcaatagtta
      481 agaggaaagc cctcaagcta gatatattga ccgcgttacg gattatcaag aggggctaac
      541 aagattattt tttgttcttt taggttgatt gtggtactgt tgatatacat gtatagcaaa
      601 tggtgttcgg gaggtttata tgcttgctat ccggttatct gatgagattg agtcccgtct
      661 ggactcgctg gcgaagcaaa ccggcagaac aaagacgttt tatgcgcggg aagcaatact
      721 ggcgcatctg gaagacctgg aggattatta tctttcagca gaaactgctg cacgcgttcg
      781 ccgtggtgat gaagcagtgc attcgtctga agacgtgagg aagtcacttg gtctggacga
      841 ttaactattc cgatcgggcg ctcaaatcgt tacgcaagat ggacaaacag aacgcacgac
      901 ggattgtgga ttttatgagt ttacgcattg cagttgctgc cgatcctcgc cagtcaggga
      961 agccgctcaa aggtgagctg ggcgagttct ggcgctatcg ggtgggagat tatcgcgttc
     1021 tgtgtgagat ccgagatgac gagcttgtta tccttgccgc cacgattgga catcgccgcg
     1081 aagtttacga ctgaagcccg ctatgcgggc ttttttgatg ccgacaacag gtattatggt
     1141 aaatactcga ctaactacgt gttctactgt ttgtcggttt tagttaacca gcaggttgtc
     1201 ttaacgataa tgtagccgtt atttttaact aataaggtca tattgttaac gtcaggcata
     1261 ataaaagaca ttatggcctc agagttatca tcagaagcag ctctctgcat tactacttcg
     1321 attccctttt ctttgaatct ataagtatcc agttgcctat taaacttaga aagacctaca
     1381 accatactta gtttgttatc tttaacagta agagatgctt cctgagtacc attacatcgg
     1441 tagtaagcgg tgtaaccgtc agcaaaagat cgaccgggga atataaatgc taggaacgca
     1501 attaaccaat acatagttga gaatcttggt attacacatt tcactatcct ctctttcatt
     1561 tttcgttcac catactttgt tatctgattt ttaatttttc tattttataa accaagtctc
     1621 taccaaaaag gcgaaaatta tcctcaaata ataataattg tttaagaaca tccatgaaag
     1681 cagctgcttc accctcagtt atgggagcta ctctatataa catataacct gagttaaggg
     1741 tttcttttat actttcttca atgctcatta taaatattag tggattatca tttttcttcc
     1801 cccatgggtt tctaattaat ccttcgatgt actcaggtcc tttgcgtcct attgcctgcg
     1861 caatattttt tatttcatct gcgtgagggc tttgttgtac agctttgttt atgcctgctt
     1921 gagtgatttt cagagactct acaccgtgta tgttgtatga gataaaatgt aataaattaa
     1981 gatgcaatgc aattaatcca tttttgttta catctgagta tttggataaa aaacgtaatg
     2041 aagtgttaac tctcccttga agcctaacaa ttgggagtgc tgccttgtat atttccttct
     2101 ccgttaaata ccgtttaaaa aacataattc tccgcctccg tatagcgtgc ctattttatt
     2161 tcgtcgtaaa acttagctct gaaatattcc tttagaacca tcatgtcatt aataattgaa
     2221 tctatctcat ccattttttc ttggtattct tttaatggtg aagggagcgg taactcaata
     2281 tttgaaaagt gattggcgag atagggatca aggtttctca tccattcagg cttttcgcca
     2341 atgcgttctt caactgaaag tgaacctgat aatctctggt taataagatc acttagactc
     2401 attttggttt tattcgaaag ccgttgtaag gattgcttta atcctatatc tagcatgata
     2461 tttgttcttt ctctcattac ccccccttgc tattgcgttt tttgttgcgc acaaagctac
     2521 cattgcgcat taagaaacgc aataaaaaag gagggaggga gtggtttgta catgatgtgc
     2581 agtgcgctga gggcatcagt ggcgggtgtt tttccgtggg atggatggag ctccggggat
     2641 aacagaactg acgatcattt aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2701 cttgcactgt aaacgaaaaa accgcctggg gaggcggttt gatcgaaggt taagtcagtt
     2761 ggggaactgc ttaaccgggt aactgggtat acgagaccgc agataccaaa actgttcttt
     2821 cagtgtagcc gcagttaggc acccacttca agacctctca atatctctct cgtatatatc
     2881 cagttaccaa tggctgctgc cagtggcgtt tttgcgtgtc cgtccgggtt ggactcaaga
     2941 tgatagttac cggaaagggc gcagcagtcg ggctgaacgg ggggttcgtg catacagccc
     3001 agcttggagc gaacgaccta aaccgagccg agataccagc agcgtgagct atgagaaagc
     3061 gccacgcttc ccgaaaggga aaaaggcgga acaggtatcc ggtaaacggc agggtcggaa
     3121 caggagagcg cacgagggag ccgccggaga gaaatcaccg gcatctttaa gtcctgtcgg
     3181 gtttcgtcct cctctgactt gagcgtccat ttctgtgatg ctcgtcaggg gaggcggagc
     3241 ctatggaaaa acggcaacgc cgcatccttt tctccggccc tttctgattt gagcgccttt
     3301 gctttatccc gttatccgtg agtcggtcac gcccgccgca gccgaacggc aggagcgcag
     3361 ctaccgagtg agcgaggagg cgtcatttca tctgtccgtg catggtattg cggacgctcc
     3421 tgagccacat atcacacctg ccacatgagg cactttcatg aatcgtgctc tcatgccaac
     3481 atagtaagcc agtatacaca gcgtaaacgc tgtgactggt tcagggctgc gccccgaaac
     3541 ccgctaacac cactgacgcg ctggcgcttg tccaacgccg gagcgacccg gaaaactttt
     3601 ccgtgccgtc cggcgtttgc gaaggaggga taatgcagaa gccgaagaag aagccgctcg
     3661 tcgtaaaaaa aagatcctgg catccggcag tgatggcaaa tctgatgcgt caggcacagg
     3721 aggaagatta ccgccgggag cagatggcag agcttgagcg ttcagcccgg tcacattttg
     3781 cggatatcgg aggagaggat ggcggttaag cgtacccgtt ttttaggtat ccgggtgacc
     3841 gatggggagt accagcagct gctggagcgc tgcaatggaa ggcaactggc ggtgtggatg
     3901 cgggaaacct gtctcgatac gcgcccggcg cggtcgttgc ggctgccgtc catcgatccg
     3961 gttttgctgc gccagcttgc cgggatgggg aacaacctca accagatcgc ccggaaaatt
     4021 aacggcggcc agtggtcagg cgctgaccgg gttcaggtgg tggccgcgct gatggccatt
     4081 gatgccgaac tcgagcggct gcggcatgcc gtgctggaaa agggggcaga cgatgatcgt
     4141 taagtttcat ccgcgagggc gaggcggcgg tgccgggccg gtggattatc tgctggggaa
     4201 agaccgccag cgcgacgggg ccatcgtcct gcagggaaag ccggaggagg tccgggagct
     4261 tatcgacgcc tcgccctacg ccaaaaagta cacctccggc gtcctgtctt ttgctgaaca
     4321 ggatttaccg cccggccagc gtgaaaagct gatggcgagc ttcgagcggg ttctgatgcc
     4381 cggactcgat aaagaccagt acagcgtgct gtgggttgaa caccgggaca aggggcggct
     4441 ggaactgaat tttctggtac cgaacacgga actgctgacc ggcaggcgtc tccagccgta
     4501 ctacgaccgg gcagaccgtc cgcgcatcga tgcgtggcag accgtggtga acgggcgact
     4561 ggggctgcac gacccgaacg caccggagaa ccggcgcgcg ctggtgacgc catcttcgtt
     4621 acccaaaacg aagcaggaag ccgcagaggc tattacgcgg ggcttactgg ccctcgcctc
     4681 gtcaggggag cttaaaacgc gtcaggacgt cactgaggcg ctggaaagcg caggttttga
     4741 ggtcgtgcgc accacaaaga gcagcatcag cattgccgac ccggacggag ggcgaaacat
     4801 ccgacttaag ggagccatct atgaacagtc ttttaacgct ggcgaaggac ttagagcaga
     4861 aatcgaaagc gcagcagcag agtaccggcg aaatgctgaa agccgcattc agcgagcacg
     4921 agaagtctgt cagagcggaa ctgagcgaaa gcgggaagag aatcagcgcc gccatccgcg
     4981 cccacgagca gggtatgagc gcggccatgc agtcgaaccg cctgagcgtg atgcgtatgg
     5041 tcgggcggac atggctgacc atcacgatgg tgtcaggact gctgttcgcc agcctgagcg
     5101 gggtgttgtg gtatcagggg agcttgatag cgtcaaatct ggccgaaatc gaccggcaga
     5161 acgcagcgct gtcgaagctg aacgcgaaga cctggggcgt gacgtacctg gaagacagca
     5221 acggacgttt tctggtgctg ccgaagggga cggccgtaga caggacgcag agctggacgg
     5281 tcgggaacgg gcggtcgaag cagaacgcgc tgagactggt gaaggagtaa gtgccgatga
     5341 ccgagctgga aaaacagttg ctgagcgcat tagagcagct acagcaggac tactcgaaaa
     5401 ggctggacga gtgggagagc gccttcgggg aatggcggaa aatgtcaggg cttatgcaac
     5461 gggagaacgc ggcgctgagc gagcgcgtga cgcgcttgag tcagcaggtg gaacgcttga
     5521 gcgggcaact gcagcgtttg agccggtagt gcagcgtcat gagatggccg tggccgcaga
     5581 acgggcgcac gagcaa
//
