LOCUS       NZ_CP127233            10060 bp    DNA     circular CON 20-JUN-2023
DEFINITION  Klebsiella pneumoniae strain 130119 plasmid p2_130119, complete
            sequence.
ACCESSION   NZ_CP127233
VERSION     NZ_CP127233.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35631498
            Assembly: GCF_030284505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Gong,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 130119
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Gong,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 18:44:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,884
            CDSs (total)                      :: 5,762
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 68 of 180
            Pseudo Genes (incomplete)         :: 121 of 180
            Pseudo Genes (internal stop)      :: 33 of 180
            Pseudo Genes (multiple problems)  :: 34 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127233.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130119"
                     /isolation_source="tracheal secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_130119"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-03-09"
     gene            join(9497..10060,1..1401)
                     /locus_tag="QRA14_RS29305"
                     /old_locus_tag="QRA14_29310"
     CDS             join(9497..10060,1..1401)
                     /locus_tag="QRA14_RS29305"
                     /old_locus_tag="QRA14_29310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1401..2132
                     /gene="mobC"
                     /locus_tag="QRA14_RS29310"
                     /old_locus_tag="QRA14_29315"
     CDS             1401..2132
                     /gene="mobC"
                     /locus_tag="QRA14_RS29310"
                     /old_locus_tag="QRA14_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2139..2669
                     /locus_tag="QRA14_RS29315"
                     /old_locus_tag="QRA14_29320"
     CDS             2139..2669
                     /locus_tag="QRA14_RS29315"
                     /old_locus_tag="QRA14_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2696..2875)
                     /locus_tag="QRA14_RS29320"
                     /old_locus_tag="QRA14_29325"
     CDS             complement(2696..2875)
                     /locus_tag="QRA14_RS29320"
                     /old_locus_tag="QRA14_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2901..3329)
                     /locus_tag="QRA14_RS29325"
                     /old_locus_tag="QRA14_29330"
     CDS             complement(2901..3329)
                     /locus_tag="QRA14_RS29325"
                     /old_locus_tag="QRA14_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3493..3807)
                     /locus_tag="QRA14_RS29330"
                     /old_locus_tag="QRA14_29335"
     CDS             complement(3493..3807)
                     /locus_tag="QRA14_RS29330"
                     /old_locus_tag="QRA14_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3795..4073)
                     /locus_tag="QRA14_RS29335"
                     /old_locus_tag="QRA14_29340"
     CDS             complement(3795..4073)
                     /locus_tag="QRA14_RS29335"
                     /old_locus_tag="QRA14_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4248..4613)
                     /locus_tag="QRA14_RS29340"
                     /old_locus_tag="QRA14_29345"
     CDS             complement(4248..4613)
                     /locus_tag="QRA14_RS29340"
                     /old_locus_tag="QRA14_29345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4610..4981)
                     /gene="tolA"
                     /locus_tag="QRA14_RS29345"
                     /old_locus_tag="QRA14_29350"
     CDS             complement(4610..4981)
                     /gene="tolA"
                     /locus_tag="QRA14_RS29345"
                     /old_locus_tag="QRA14_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5255..5500)
                     /locus_tag="QRA14_RS29350"
                     /old_locus_tag="QRA14_29355"
     CDS             complement(5255..5500)
                     /locus_tag="QRA14_RS29350"
                     /old_locus_tag="QRA14_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5827..7512
                     /locus_tag="QRA14_RS29355"
                     /old_locus_tag="QRA14_29360"
     CDS             5827..7512
                     /locus_tag="QRA14_RS29355"
                     /old_locus_tag="QRA14_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7522..7779
                     /locus_tag="QRA14_RS29360"
                     /old_locus_tag="QRA14_29365"
     CDS             7522..7779
                     /locus_tag="QRA14_RS29360"
                     /old_locus_tag="QRA14_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7864..8013
                     /locus_tag="QRA14_RS29365"
                     /old_locus_tag="QRA14_29370"
     CDS             7864..8013
                     /locus_tag="QRA14_RS29365"
                     /old_locus_tag="QRA14_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             827..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8117..8695)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1402..2129
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga aatgacagac
       61 gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccacg
      121 gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg
      181 ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc
      241 ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgct
      301 gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc
      361 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc
      421 gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc
      481 ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag
      541 cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc gttgccggag
      601 gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc
      661 gtcctgcaga cggacagcgg tatcgatgtg gcggggataa tcaacggcgg cggctgcctg
      721 tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc
      781 cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc
      841 atgctcgatg agattaaata tctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg
      901 cgcagccggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt cggtcagtgc
      961 gggcaggatc tgcccgctga ttttgtgaaa accacggtac tggataacac gccgatccgc
     1021 tggttctatc gcgcggccag ccaggagtcc gcgcagtggg cggcgggcca gaccggggag
     1081 atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt ggagcacatc
     1141 agcggcgaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa
     1201 cacctgcctg acgggttcgc ggtgatgacc gggctgggcg tggcgcggct ggcgttcagc
     1261 agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa ccttcccggt gctggcgaaa
     1321 acggacccgc tggcggagta cctgcctccg gccgacggcg gcggaccaaa agcggatgat
     1381 gattttgcgg ggctttactg atgacactgg agcagtacaa cgtcagccac gcgaagcggc
     1441 aggcgcggaa cgcggagaaa acacggctga ccctgcgctg gctgcgggaa gagctgtgtt
     1501 ccacggcgga gctggttgcg cgacggctgg ggattgcggc ggtacagccg gtttaccgct
     1561 tcctggacag tctggtggcg aaagggctgc tggtccgggc gaaatacccg gttgacggcc
     1621 gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc gttctcgttc gacgaggacg
     1681 agccgctgac ggacgttatt ccgtttcagc cgtcaagggt gtcggcggcg cagctgccgc
     1741 accggctggc cgtgcagtcc ctgcgcctct ccatggaggg ccggggggcc accggctggc
     1801 ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc ggacgcgctg gcggagctgg
     1861 acggcagaac ggtggcattt gaggtagagc gcaccgtcaa gagccgcagg cggtatcagg
     1921 aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg catcgatgaa atctggtaca
     1981 tctgccctga ccggccgacg cttgtccggg tacagcgggc gattctggcg gtggatgaaa
     2041 tcgtgaatcc acagaccggc gaggcccgca aaacggtgga gctggaccgg gagaggctgt
     2101 tcgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac atggtattta
     2161 ttgaggacgt ggaagacaaa gacggcgagc gctgcattat gccaaccagc gagcatctgg
     2221 aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg
     2281 cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc aacagcgacg
     2341 aaaaagccac gtactttttc accggtgaaa atccgcaatg ggaacgggac aaacccacag
     2401 gaatgaagct gtgtgattac ctctatcgaa aggtggagcg gttcgccatc gagaacggtg
     2461 tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg attgaggtgg
     2521 tcagcgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag ctgatgcggg
     2581 aatataacag ccgctatttt aacgtgccgg tgcctgaact gccggaggac aaacccgccc
     2641 gccggtggtg gccgttcggc aaaaaataac gccagcaccg gcatggccgg tgctgttatt
     2701 cttccccgag cttatcccgc atcacctgcc agagtgtttc cgcatcctcg tgaagccgtt
     2761 cgcacatgtc ggccggggta tccatgtcca gctcgttcag cttttccagc aacagcagcg
     2821 actgatcctt aatgaattta gccatattga gggcagtttt ttcctgcttc gtcatgttct
     2881 ccattccctt cggtaagttc tcagctaaac cagccggacc agaagcgttt tttcttctgt
     2941 gtcagttctg ttgtcaatct gtccacttcg gcctggagtt tccggcgctc atcatcgtgc
     3001 tggcggcgtt cttcttcccg ggatgtttta tcttccagca tcagggtgac agcctgccgg
     3061 agatcggcca gttccttttg cattgccaga agctgttcct gtgggaaatt tttttgtgta
     3121 ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga tattcacatt tccgtataca
     3181 cggatcagtt ctgacacatc cacgacaggg ttgtttttcc cgtcacgtga cacggtaacc
     3241 tttccctgtt ttacatgatt gtatagcgtt cttctggtaa tgcccgcggc ttgtgctgcc
     3301 tgtgaaaggt tgagcaatgt tttcgccact gtatacccct gattttatcg ggtgtgaaag
     3361 gtgtgtaatt tctcacggta aatgtatcgc tgaattttac ccgtttttcc gttcaggatg
     3421 tggttttctg acgcgctgcg cttgtcaaac cggcgacggc cacgcaatga attgcgcccc
     3481 cgtcatcggt tattagaggc tgtctgcccc cggatacccg gattctttct gatgcctgac
     3541 ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg tagagcatca catagccact
     3601 gtcgccaaac ccgatcacca gttccttata ttccagcggc agaaacggca ccgggcggcc
     3661 aatgtctggc aatgttttca gttgctggat agcccggacg atcacctcac ctccttttct
     3721 ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg cgttgtaaat cgcgctgtgc
     3781 cagcgcggaa atcgttacct gtggcatggc ggagcatcct gctcgttctc agttccccag
     3841 gtacttatcc aggcttccgc ttcttccgca gtaaggtgca gacccgtttc ctgatagtgc
     3901 tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct tctcttcccg gtcgatgtat
     3961 tcggtgattg cctccagcat tagcgcgtgc gctgagcggt gccggtcatc ggccagcgtt
     4021 ttcagccggt ccttaagttc ttcatcgagc ctgatggatg ttgctgatga cataaaagcc
     4081 cctttgtagt catatgtatt acacatgact actttaatcc gtggcggcgc tatttgccag
     4141 aaatacggct tttgctgccc atttttcagc ttcgcttcca ctaatcaggg ctgctacagg
     4201 ctcactcatc atccaggact attgcagcct gcgggttaaa ggacctgtta ctggcggcaa
     4261 ccatcatcat tgagcttttg ggtggtgact ggtcgcggat cctgtaagcc gtcatttttc
     4321 agactgaatt caaaatttat aacctgtcgc attgccgggt gccctgcggg atatagccag
     4381 tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac agccggtaag cgacacgttg
     4441 tccactcgtc catcgtccag aatatcaaac gtggcggtca cctgaccttc gatgcgtttt
     4501 gctgcggcct gtaccgggta ggttggctgc agtgtggtta cgggttttag tggtatcgca
     4561 tgtaccggag tgaggaacat ggccgccagc actgtcagag taaaagcttt catggtctga
     4621 aatccagagg ggcgtttttg aatatttgcc aggtattttc atccggtgcc ggcggaattt
     4681 tggcgtgggt aacggctgat attgctgctt tacagagttt cggatctcct ccttcagcaa
     4741 tggcgctgtt cagagtgcca tcacgttgta tggatatccg cacggtgcac atttttcccg
     4801 cataggcctc tggttcaaac atctgcattt caacagcggt cttgatggcc gcagcgtagc
     4861 tactgatttt agatgactta tccagagtat aaattttcgg cggctgggtg gtggaatgct
     4921 tacaggccgt caggagtaag gggctgcaca atgccagcag gataaagggt attaaacgca
     4981 tcgggttgat gacctgtcag taaaaaatgt tacatgtata tttgtcactc tttacggcca
     5041 cgttcattta cataaacacg gcttatcgta tcgtttttac accaaacggc ccgccatgca
     5101 gttgcgtgca taggcatgca gcgggttaca atttaccggg cgcgtttccg gcggttttcc
     5161 gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa cgccctcaga ccgtctgagc
     5221 ggtgcgggca atgcgacgtt atggtaaata ccccttaccg gcggcgctgt ggctgccagg
     5281 tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat ttcctgcgcc cgcctggctt
     5341 cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac ctgttgtttt accgcctttg
     5401 gcagcgtttt gttccctttc aggcccatgg ccagtgcctg agcgacaaag gcgtccgcca
     5461 ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat agtggcctcc gggcgtctca
     5521 ggtttcccga acagtttaac cgcaggtcct ctcctcatgc cagttagctt tcagatgtgt
     5581 ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat aataaaattt gttgccatga
     5641 gaaattttcg ggaaaagacg cgatctgctt acaggtggct ccgggcgctt ttttgtgcct
     5701 gaagatgaga actaatctca tatattgaca cctgaaaacg ggaggagtaa ggtaataatt
     5761 atactgtgta tatgtacagt atgttggctt taatttattg ttttaaatgt caaagaggaa
     5821 aacggtatga gtggtggaga cggtcgaggt ccgggtaatt caggtctggg acataatggt
     5881 ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag gtggcccttc atcaggtggt
     5941 ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacgccgaa tggagatatt
     6001 cataactata atccggggga gtttggtaac ggcgggagta aacccggtgg taatggtggt
     6061 aacagcggca atcatagcgg tagctctggt ggcggacagt cttcggccac tgcgatggcc
     6121 ttcggtctgc ctgccctggc tactccgggg gctgaaggac tggctttatc agtttccggc
     6181 gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt
     6241 ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg agattgcaaa agatgatccg
     6301 aaaatgatgt caaaaattat gacgtcatta ccggccgatg cggtgacgga gactccggca
     6361 agtactttac cactggacca ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg
     6421 aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg
     6481 gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct
     6541 ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag ccccgccaaa aggcattgtt
     6601 gctgaaaaag gtgattcacg tccggctggt tttacagccg gtggtaactc ccgtgaggcc
     6661 gttattcgtt tcccgaaaga gaccggacag aagccggttt atgtgtcggt gacagatgtt
     6721 cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg
     6781 gacgccgctc atccggaaga ggggctgaaa agagactatg ataaagcgaa agctgagctg
     6841 gatgccgaag ataaaaatat tgcgacctta aacagccgca ttgcatcgac agagaaggcg
     6901 ctccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca
     6961 aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat
     7021 caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa agcggctcag
     7081 gcatctttaa acgcaatgaa tgaatcctta tccagagata aggctgcact taccggggcg
     7141 atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa gttaaatgag
     7201 gaaaagaaca agcctcgcaa ggggactaaa gactacggcc atgattatca tccagtacct
     7261 aaaacagaag acataaaggg acttggagag ttaaagcgag gtgatcctaa aacacctaaa
     7321 caaggtggtg gtggtaagcg ggcccgatgg tatggcgata aaaaacgtaa aatttatgaa
     7381 tgggattccc agcacggtga gcttgaaggg tatcgtgcca gtgatggcga acacctaggc
     7441 gcatttgatc ccaaaacggg taagcaggtt aaagggccag atccaaaacg gaacattaaa
     7501 aaatatcttt gagaggtaaa tatggggctt aaattacgtt tgacatggtt tgataagaaa
     7561 actgaagagt ttaaagggga agagtattct aaagactttg gtgacgatgg ttctgtcatt
     7621 gaaagcttgg gaatgccttt aaaggataat attaataatg gtgagtttga tttgaagaaa
     7681 gaatgggttc ctttactaca gccatttttt aaaaatcaaa taaaacctga tgtggataat
     7741 tgttatatag cttttgatta tcgggacggt aactggtaac aaaaaattat caaggatgat
     7801 ttgaaatact gacttccggt gttcagggat gaatgccgga gtctttaaat acagggaaag
     7861 tatatgaaaa aggtaaagac tatttttctt tttatattga ttgttgctgg ctttcttctg
     7921 gccgcatgtc aggcaaatta tatccgggat gttcagggcg gaacggtggc accatcgtcc
     7981 tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc
     8041 ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt
     8101 tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc
     8161 acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac
     8221 cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg
     8281 gcgttttgcg tgctttcccg ggttggactc aagtgaacag ttaccggata aggcgcagca
     8341 gtcgggctga acggggagtt cttgcttaca gcccagcttg gagcgaacga cctacaccga
     8401 gccgagatac cagtgtgtga gctatgagaa agcgccacac ttcccgcaag ggagaacggc
     8461 ggacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg agcgacctac
     8521 cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc cgtactgtca gattcatggt
     8581 tgagcctcac ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actctggcag
     8641 gagggcggag cctatggaaa aacgccaccg gcgcggccct gccgttttgc ctcacatgtt
     8701 agtcccctgc ttatccacgg aatctgtggg taactttgta tgtgtccgca gcgcccgccg
     8761 cagtctcacg cccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc
     8821 tgcaagacgg tcaccggacg cgctgatgtg ggatattgtc ccctgattaa gtctttctat
     8881 tccctgacga tcaattatca ggataggtga accatgtcgg tttttcgctg aacattttac
     8941 gttaacctat ctctgttgct tttttctcct tgcggagaaa tgcaagtcat ccccgctggg
     9001 gcgtggctga cttcagggga aaagaccttg cttacgctgg ctggacgcca ccgacgcacg
     9061 gtcttttccc ctctttctgg ctctgtccgg ctggacgccg gccacgccag aaattcaccc
     9121 ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctga
     9181 cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct
     9241 gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc
     9301 tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct
     9361 ggcctccgcg gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt
     9421 ggggcatgtc cgcggcttgt gcagcggggg ctgcgcatca cgccggtaaa cccgtcgtct
     9481 gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc atatcccgga
     9541 gctggcccgg cagatttacc cgtgggtcag ctggctgtac cgggagtatt acagtctggc
     9601 cggttttttt ctggttatcg gcctgttttt cggtctggct gaactctggg ccggccgaat
     9661 gcgttttgtg gggctgagcc ataaggcgcg gcgggagtat gccgatcgct ataagcgcga
     9721 taataaccgg actgtgccat tttcggtgct ggcctcgctg gattcacccg ggcggatggt
     9781 cagggcacgg cgacatgtct ggaactacgc gtactggctg accggcgctg ccggtctggc
     9841 ccttctgggg gggctggttg tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg
     9901 cccggacctc tatgcgaacg cagattatta cctgctgcag ccggctaacc tgttcgggat
     9961 agcctggtgt gccggagggc ttgtcgccgg cgtccttctg gcgcggctgc tggcgttcct
    10021 gctgcttgat ggtcattttg cggccgctga cgaggcggtg
//
