LOCUS       NZ_CP127158             5596 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain T1 plasmid pT1_p5,
            complete sequence.
ACCESSION   NZ_CP127158
VERSION     NZ_CP127158.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35562370
            Assembly: GCF_030263875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhou,A.
  TITLE     Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) strains isolated
            from Shanghai East Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhou,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2023) Medical Laboratory, Shanghai East Hospital,
            Yuntai Road 1800, Shanghai, Shanghai 200123, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 16:12:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,005
            CDSs (total)                      :: 5,880
            Genes (coding)                    :: 5,693
            CDSs (with protein)               :: 5,693
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 69 of 187
            Pseudo Genes (incomplete)         :: 126 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 31 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127158.1.
            Bacteria and source DNA available from Shanghai East Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T1"
                     /isolation_source="Shanghai East Hospital"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pT1_p5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.11 N 121.29 E"
                     /collection_date="2021"
                     /collected_by="Shangai East Hospital"
     gene            661..885
                     /locus_tag="QP513_RS29980"
                     /old_locus_tag="QP513_29980"
     CDS             661..885
                     /locus_tag="QP513_RS29980"
                     /old_locus_tag="QP513_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            869..1135
                     /locus_tag="QP513_RS29985"
                     /old_locus_tag="QP513_29985"
     CDS             869..1135
                     /locus_tag="QP513_RS29985"
                     /old_locus_tag="QP513_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1205..1555)
                     /locus_tag="QP513_RS29990"
                     /old_locus_tag="QP513_29990"
     CDS             complement(1205..1555)
                     /locus_tag="QP513_RS29990"
                     /old_locus_tag="QP513_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149795812.1"
                     /translation="MYWLITFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1621..2166)
                     /locus_tag="QP513_RS29995"
                     /old_locus_tag="QP513_29995"
     CDS             complement(1621..2166)
                     /locus_tag="QP513_RS29995"
                     /old_locus_tag="QP513_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2192..2518)
                     /locus_tag="QP513_RS30000"
                     /old_locus_tag="QP513_30000"
     CDS             complement(2192..2518)
                     /locus_tag="QP513_RS30000"
                     /old_locus_tag="QP513_30000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3674..3850
                     /locus_tag="QP513_RS30005"
                     /old_locus_tag="QP513_30005"
     CDS             3674..3850
                     /locus_tag="QP513_RS30005"
                     /old_locus_tag="QP513_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3840..4184
                     /locus_tag="QP513_RS30010"
                     /old_locus_tag="QP513_30010"
     CDS             3840..4184
                     /locus_tag="QP513_RS30010"
                     /old_locus_tag="QP513_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4393..>4827
                     /locus_tag="QP513_RS30015"
                     /old_locus_tag="QP513_30015"
                     /pseudo=""
     CDS             4393..>4827
                     /locus_tag="QP513_RS30015"
                     /old_locus_tag="QP513_30015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4862..5371
                     /locus_tag="QP513_RS30020"
                     /old_locus_tag="QP513_30020"
     CDS             4862..5371
                     /locus_tag="QP513_RS30020"
                     /old_locus_tag="QP513_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5378..5590
                     /locus_tag="QP513_RS30025"
                     /old_locus_tag="QP513_30025"
     CDS             5378..5590
                     /locus_tag="QP513_RS30025"
                     /old_locus_tag="QP513_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3509..3588
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2926..3323)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4175..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.789"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtcatgagat ggccgtggcc gcagaacggg cgcacgagca acggcagcac gaaaaggaac
       61 tgacggaagc gcgttcccgg cagcgaagct atgacggtcc gtcgctgggc tgaaatgcag
      121 gaaaacaggg ttctcatcgg gcatttacgc tgaaaccgga tgtcattcac ctggctgcat
      181 ggctatgcag ccaggtaaaa tttatctgtc gcgaacgggg cggtttatcg ggtggtttgt
      241 tgccggtttt acctgactgc caccccgttc gcggcgaacc cgtccggggc ggtgcgggca
      301 acgggtgtta tgtttaatca gcgtgaacgg cagatacaaa aaagccccgg agggctttta
      361 actactgact tatttttttg aagatttgct gggtttcaat ttgtcagtat ttcccggcgt
      421 ttttttattg tcacgctggc gtttatcagg ttcccctgtt gaatcggcaa ttcgtttcgg
      481 acccggctta agacccataa tcgttatcct ttcaatagtt aagaggaaag ccctcaagct
      541 agatatattg accgcgttac ggattatcaa gaggggctaa caagattatt ttttgttctt
      601 ttaggttgat tgtggtactg ttgatataca tgtatagcaa atggtgttcg ggaggtttat
      661 atgcttgcta tccggttatc tgatgagatt gagtcccgtc tggactcgct ggcgaagcaa
      721 accggcagaa caaagacgtt ttatgcgcgg gaagcaatac tggcgcatct ggaagacctg
      781 gaggattatt atctttcagc agaaactgct gcacgcgttc gccgtggtga tgaagcagtg
      841 cattcgtctg aagacgtgag gaagtcactt ggtctggacg attaactatt ccgatcgggc
      901 gctcaaatcg ttacgcaaga tggacaaaca gaacgcacga cggattgtgg attttatgag
      961 tttacgcatt gcagttgctg ccgatcctcg ccagtcaggg aagccgctca aaggtgagct
     1021 gggcgagttc tggcgctatc gggtgggaga ttatcgcgtt ctgtgtgaga tccgagatga
     1081 cgagcttgtt atccttgccg ccacgattgg acatcgccgc gaagtttacg actgaagccc
     1141 gctatgcggg cttttttgat gccgacaaca ggtattatgg taaatactcg actaactacg
     1201 tgttctactg tttgtcggtt ttagttaacc agcaggttgt cttaacgata atgtagccgt
     1261 tatttttaac taataaggtc atattgttaa cgtcaggcat aataaaagac attatggcct
     1321 cagagttatc atcagaagca gctctctgca ttactacttc gattcccttt tctttgaatc
     1381 tataagtatc cagttgccta ttaaacttag aaagacctac aaccatactt agtttgttat
     1441 ctttaacagt aagagatgct tcctgagtac cattacatcg gtagtaagcg gtgtaaccgt
     1501 cagcaaaaga tcgaccgggg aatataaatg ctaggaacgt aattaaccaa tacatagttg
     1561 agaatcttgg tattacacat ttcactatcc tctctttcat ttttcgttca ccatactttg
     1621 ttatctgatt tttaattttt ctattttata aaccaagtct ctaccaaaaa ggcgaaaatt
     1681 atcctcaaat aataataatt gtttaagaac atccatgaaa gcagctgctt caccctcagt
     1741 tatgggagct actctatata acatataacc tgagttaagg gtttctttta tactttcttc
     1801 aatgctcatt ataaatatta gtggattatc atttttcttc ccccatgggt ttctaattaa
     1861 tccttcgatg tactcaggtc ctttgcgtcc tattgcctgc gcaatatttt ttatttcatc
     1921 tgcgtgaggg ctttgttgta cagctttgtt tatgcctgct tgagtgattt tcagagactc
     1981 tacaccgtgt atgttgtatg agataaaatg taataaatta agatgcaatg caattaatcc
     2041 atttttgttt acatctgagt atttggataa aaaacgtaat gaagtgttaa ctctcccttg
     2101 aagcctaaca attgggagtg ctgccttgta tatttccttc tccgttaaat accgtttaaa
     2161 aaacataatt ctccgcctcc gtatagcgtg cctattttat ttcgtcgtaa aacttagctc
     2221 tgaaatattc ctttagaacc atcatgtcat taataattga atctatctca tccatttttt
     2281 cttggtattc ttttaatggt gaagggagcg gtaactcaat atttgaaaag tgattggcga
     2341 gatagggatc aaggtttctc atccattcag gcttttcgcc aatgcgttct tcaactgaaa
     2401 gtgaacctga taatctctgg ttaataagat cacttagact cattttggtt ttattcgaaa
     2461 gccgttgtaa ggattgcttt aatcctatat ctagcatgat atttgttctt tctctcatta
     2521 cccccccttg ctattgcgtt ttttgttgcg cacaaagcta ccattgcgca ttaagaaacg
     2581 caataaaaaa ggagggaggg agtggtttgt acatgatgtg cagtgcgctg agggcatcag
     2641 tggcgggtgt ttttccgtgg gatggatgga gctccgggga taacagaact gacgatcatt
     2701 taaaggatct tcttgagatc ctttttttct gcgcgtaatc tcttgcactg taaacgaaaa
     2761 aaccgcctgg ggaggcggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccggg
     2821 taactgggta tacgagaccg cagataccaa aactgttctt tcagtgtagc cgcagttagg
     2881 cacccacttc aagacctctc aatatctctc tcgtatatat ccagttacca atggctgctg
     2941 ccagtggcgt ttttgcgtgt ccgtccgggt tggactcaag atgatagtta ccggaaaggg
     3001 cgcagcagtc gggctgaacg gggggttcgt gcatacagcc cagcttggag cgaacgacct
     3061 aaaccgagcc gagataccag cagcgtgagc tatgagaaag cgccacgctt cccgaaaggg
     3121 aaaaaggcgg aacaggtatc cggtaaacgg cagggtcgga acaggagagc gcacgaggga
     3181 gccgccggag agaaatcacc ggcatcttta agtcctgtcg ggtttcgtcc tcctctgact
     3241 tgagcgtcca tttctgtgat gctcgtcagg ggaggcggag cctatggaaa aacggcaacg
     3301 ccgcatcctt ttctccggcc ctttctgatt tgagcgcctt tgctttatcc cgttatccgt
     3361 gagtcggtca cgcccgccgc agccgaacgg caggagcgca gctaccgagt gagcgaggag
     3421 gcgtcatttc atctgtccgt gcatggtatt gcggacgctc ctgagccaca tatcacacct
     3481 gccacatgag gcactttcat gaatcgtgct ctcatgccaa catagtaagc cagtatacac
     3541 agcgtaaacg ctgtgactgg ttcagggctg cgccccgaaa cccgctaaca ccactgacgc
     3601 gctggcgctt gtccaacgcc ggagcgaccc ggaaaacttt tccgtgccgt ccggcgtttg
     3661 cgaaggaggg ataatgcaga agccgaagaa gaagccgctc gtcgtaaaaa aaagatcctg
     3721 gcatccggca gtgatggcaa atctgatgcg tcaggcacag gaggaagatt accgccggga
     3781 gcagatggca gagcttgagc gttcagcccg gtcacatttt gcggatatcg gaggagagga
     3841 tggcggttaa gcgtacccgt tttttaggta tccgggtgac cgatggggag taccagcagc
     3901 tgctggagcg ctgcaatgga aggcaactgg cggtgtggat gcgggaaacc tgtctcgata
     3961 cgcgcccggc gcggtcgttg cggctgccgt ccatcgatcc ggttttgctg cgccagcttg
     4021 ccgggatggg gaacaacctc aaccagatcg cccggaaaat taacggcggc cagtggtcag
     4081 gcgctgaccg ggttcaggtg gtggccgcgc tgatggccat tgatgccgaa ctcgagcggc
     4141 tgcggcatgc cgtgctggaa aagggggcag acgatgatcg ttaagtttca tccgcgaggg
     4201 cgaggcggcg gtgccgggcc ggtggattat ctgctgggga aagaccgcca gcgcgacggg
     4261 gccatcgtcc tgcagggaaa gccggaggag gtccgggagc ttatcgacgc ctcgccctac
     4321 gccaaaaagt acacctccgg cgtcctgtct tttgctgaac aggatttacc gcccggccag
     4381 cgtgaaaagc tgatggcgag cttcgagcgg gttctgatgc ccggactcga taaagaccag
     4441 tacagcgtgc tgtgggttga acaccgggac aaggggcggc tggaactgaa ttttctggta
     4501 ccgaacacgg aactgctgac cggcaggcgt ctccagccgt actacgaccg ggcagaccgt
     4561 ccgcgcatcg atgcgtggca gaccgtggtg aacgggcgac tggggctgca cgacccgaac
     4621 gcaccggaga accggcgcgc gctggtgacg ccatcttcgt tacccaaaac gaagcaggaa
     4681 gccgcagagg ctattacgcg gggcttactg gccctcgcct cgtcagggga gcttaaaacg
     4741 cgtcaggacg tcactgaggc gctggaaagc gcaggttttg aggtcgtgcg caccacaaag
     4801 agcagcatca gcattgccga cccggacgga gggcgaaaca tccgacttaa gggagccatc
     4861 tatgaacagt cttttaacgc tggcgaagga cttagagcag aaatcgaaag cgcagcagca
     4921 gagtaccggc gaaatgctga aagccgcatt cagcgagcac gagaagtctg tcagagcgga
     4981 actgagcgaa agcgggaaga gaatcagcgc cgccatccgc gcccacgagc agggtatgag
     5041 cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg gtcgggcgga catggctgac
     5101 catcacgatg gtgtcaggac tgctgttcgc cagcctgagc ggggtgttgt ggtatcaggg
     5161 gagcttgata gcgtcaaatc tggccgaaat cgaccggcag aacgcagcgc tgtcgaagct
     5221 gaacgcgaag acctggggcg tgacgtacct ggaagacagc aacggacgtt ttctggtgct
     5281 gccgaagggg acggccgtag acaggacgca gagctggacg gtcgggaacg ggcggtcgaa
     5341 gcagaacgcg ctgagactgg tgaaggagta agtgccgatg accgagctgg aaaaacagtt
     5401 gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg agtgggagag
     5461 cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa cgggagaacg cggcgctgag
     5521 cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg agcgggcaac tgcagcgttt
     5581 gagccggtag tgcagc
//
