LOCUS       NZ_CP127157            11971 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain T1 plasmid pT1_p4,
            complete sequence.
ACCESSION   NZ_CP127157
VERSION     NZ_CP127157.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35562370
            Assembly: GCF_030263875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Zhou,A.
  TITLE     Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) strains isolated
            from Shanghai East Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Zhou,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2023) Medical Laboratory, Shanghai East Hospital,
            Yuntai Road 1800, Shanghai, Shanghai 200123, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 16:12:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,005
            CDSs (total)                      :: 5,880
            Genes (coding)                    :: 5,693
            CDSs (with protein)               :: 5,693
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 69 of 187
            Pseudo Genes (incomplete)         :: 126 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 31 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127157.1.
            Bacteria and source DNA available from Shanghai East Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T1"
                     /isolation_source="Shanghai East Hospital"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pT1_p4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.11 N 121.29 E"
                     /collection_date="2021"
                     /collected_by="Shangai East Hospital"
     gene            join(11333..11971,1..1326)
                     /locus_tag="QP513_RS29910"
                     /old_locus_tag="QP513_29910"
     CDS             join(11333..11971,1..1326)
                     /locus_tag="QP513_RS29910"
                     /old_locus_tag="QP513_29910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1326..2057
                     /gene="mobC"
                     /locus_tag="QP513_RS29915"
                     /old_locus_tag="QP513_29915"
     CDS             1326..2057
                     /gene="mobC"
                     /locus_tag="QP513_RS29915"
                     /old_locus_tag="QP513_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2064..2594
                     /locus_tag="QP513_RS29920"
                     /old_locus_tag="QP513_29920"
     CDS             2064..2594
                     /locus_tag="QP513_RS29920"
                     /old_locus_tag="QP513_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2621..2800)
                     /locus_tag="QP513_RS29925"
                     /old_locus_tag="QP513_29925"
     CDS             complement(2621..2800)
                     /locus_tag="QP513_RS29925"
                     /old_locus_tag="QP513_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2826..3254)
                     /locus_tag="QP513_RS29930"
                     /old_locus_tag="QP513_29930"
     CDS             complement(2826..3254)
                     /locus_tag="QP513_RS29930"
                     /old_locus_tag="QP513_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3418..3732)
                     /locus_tag="QP513_RS29935"
                     /old_locus_tag="QP513_29935"
     CDS             complement(3418..3732)
                     /locus_tag="QP513_RS29935"
                     /old_locus_tag="QP513_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3720..3998)
                     /locus_tag="QP513_RS29940"
                     /old_locus_tag="QP513_29940"
     CDS             complement(3720..3998)
                     /locus_tag="QP513_RS29940"
                     /old_locus_tag="QP513_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4173..4538)
                     /locus_tag="QP513_RS29945"
                     /old_locus_tag="QP513_29945"
     CDS             complement(4173..4538)
                     /locus_tag="QP513_RS29945"
                     /old_locus_tag="QP513_29945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4535..4906)
                     /gene="tolA"
                     /locus_tag="QP513_RS29950"
                     /old_locus_tag="QP513_29950"
     CDS             complement(4535..4906)
                     /gene="tolA"
                     /locus_tag="QP513_RS29950"
                     /old_locus_tag="QP513_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5180..5425)
                     /locus_tag="QP513_RS29955"
                     /old_locus_tag="QP513_29955"
     CDS             complement(5180..5425)
                     /locus_tag="QP513_RS29955"
                     /old_locus_tag="QP513_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6070..7557
                     /gene="ltrA"
                     /locus_tag="QP513_RS29960"
                     /old_locus_tag="QP513_29960"
     CDS             6070..7557
                     /gene="ltrA"
                     /locus_tag="QP513_RS29960"
                     /old_locus_tag="QP513_29960"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7662..9347
                     /locus_tag="QP513_RS29965"
                     /old_locus_tag="QP513_29965"
     CDS             7662..9347
                     /locus_tag="QP513_RS29965"
                     /old_locus_tag="QP513_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9357..9614
                     /locus_tag="QP513_RS29970"
                     /old_locus_tag="QP513_29970"
     CDS             9357..9614
                     /locus_tag="QP513_RS29970"
                     /old_locus_tag="QP513_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9699..9848
                     /locus_tag="QP513_RS29975"
                     /old_locus_tag="QP513_29975"
     CDS             9699..9848
                     /locus_tag="QP513_RS29975"
                     /old_locus_tag="QP513_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2662..11971
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9952..10531)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.113"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1327..2054
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ttcggggagc cggtaccggt taacgcgctg gccgattttt ccacggagca ggcccggaaa
       61 cagcaggcgg tgtttctggg taaggatgag cagggccagc cggtgctggt ccctcgtgat
      121 acctggcgca agaccaatat tcagatcctc ggcctgcccg ggagcggcaa gagcgcgatg
      181 gccaccaacg cgctcattcg ctgcgtacgc gatttcgggg acgctgtggt gtacttcgac
      241 cccaaagggg acgcgtgggc gccgcacgtc ttccgggcgc actgcccgga cttcaccctg
      301 ctggatctgc ggccggggaa accggcgcag ctgaatctgt tccgcgatct cgaccagtac
      361 gccctgaaga atctgctggt tgccgggttt aacctgagtg agaccggcga cgtggcggac
      421 cactaccgca tcagcgagca gaaagcggcg aagctgattg cggagcagtt cccgcagggg
      481 gcaaacattc agcaggtgct ggcggcggcg tacgcgttgc cggaggccct gaaaaaggac
      541 gtgaaggggc tgatcaccaa gctggagaac gtggccgacc tgagcgtcct gcagacggac
      601 agcggtatcg atgtggcggg gataatcaac ggcggcggct gcctgtacgt catcggctcg
      661 atggacgatg aggcggtgat ccgggtgcag aagatgctgt ttgcccgctg cgcgcaaatc
      721 atcattgcgc gggatgagtt ccggaggtgg ccgcacgcga gcatcatgct cgatgagatt
      781 aaatatctgc tgtcgaagta cgtgctgaac gcgctgggga cgctgcgcag ccgggactgc
      841 aacctgctgc tcgcgcacca gtcgctgggc gacttcggtc agtgcgggca ggatctgccc
      901 gctgattttg tgaaaaccac ggtactggat aacacgccga tccgctggtt ctatcgcgcg
      961 gccagccagg agtccgcgca gtgggcggcg ggccagaccg gggagatccg ggtggacgtg
     1021 gagcgccgcc gggccagccg ggaggcgggg aacgtggagc acatcagcgg cgacagcttc
     1081 atccagaagg aggcgcggcc gctgtttgac gtgaacaccc tgcaacacct gcctgacggg
     1141 ttcgcggtga tgaccgggct gggcgtggcg cggctggcgt tcagcagccc gctgcgggtt
     1201 gagcgccggg aaatttcgct gaaaaccttc ccggtgctgg cgaaaacgga cccgctggcg
     1261 gagtacctgc ctccggccga cggcggcgga ccaaaagcgg atgatgattt tgcggggctt
     1321 tactgatgac actggagcag tacaacgtca gccacgcgaa gcggcaggcg cggaacgcgg
     1381 agaaaacacg gctgaccctg cgctggctgc gggaagagct gtgttccacg gcggagctgg
     1441 ttgcgcgacg gctggggatt gcggcggtac agccggttta ccgcttcctg gacagtctgg
     1501 tggcgaaagg gctgctggtc cgggcgaaat acccggttga cggccgtcag gtcagcgtgt
     1561 gggggctgac gccgcacggg gtggcgttct cgttcgacga ggacgagccg ctgacggacg
     1621 ttattccgtt tcagccgtca agggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc
     1681 agtccctgcg cctctccatg gagggccggg gggccaccgg ctggcggtac ctgcaccgta
     1741 tggccctgaa agggatgaaa gtgccggacg cgctggcgga gctggacggc agaacggtgg
     1801 catttgaggt agagcgcacc gtcaagagcc gcaggcggta tcaggaagtg gtgtccgggt
     1861 atctgttcaa ccgccgggcg aacggcatcg atgaaatctg gtacatctgc cctgaccggc
     1921 cgacgcttgt ccgggtacag cgggcgattc tggcggtgga tgaaatcgtg aatccacaga
     1981 ccggcgaggc ccgcaaaacg gtggagctgg accgggagag gctgttcgcg tgctttaaat
     2041 ttatgacaac ggagtgaggg attatggcgc tgtacatggt atttattgag gacgtggaag
     2101 acaaagacgg cgagcgctgc attatgccaa ccagcgagca tctggaagac atggtggtgc
     2161 tcgcctggca gaccggggcg tatgttttct gttttgtcga ccgggcccag catctggcga
     2221 tgatgaaatc catcgggatg tatgacgagc ggctcaacag cgacgaaaaa gccacgtact
     2281 ttttcaccgg tgaaaatccg caatgggaac gggacaaacc cacaggaatg aagctgtgtg
     2341 attacctcta tcgaaaggtg gagcggttcg ccatcgagaa cggtgtgcag gtggttgatg
     2401 ccgggaagtg ctcaccggag cagattaacg cgatgattga ggtggtcagc gagcggcacg
     2461 gggaaagcgc ggtgaacgag aagctgaaag cggagctgat gcgggaatat aacagccgct
     2521 attttaacgt gccggtgcct gaactgccgg aggacaaacc cgcccgccgg tggtggccgt
     2581 tcggcaaaaa ataacgccag caccggcatg gccggtgctg ttattcttcc ccgagcttat
     2641 cccgcatcac ctgccagagt gtttccgcat cctcgtgaag ccgttcgcac atgtcggccg
     2701 gggtatccat gtccagctcg ttcagctttt ccagcaacag cagcgactga tccttaatga
     2761 atttagccat attgagggca gttttttcct gcttcgtcat gttctccatt cccttcggta
     2821 agttctcagc taaaccagcc ggaccagaag cgttttttct tctgtgtcag ttctgttgtc
     2881 aatctgtcca cttcggcctg gagtttccgg cgctcatcat cgtgctggcg gcgttcttct
     2941 tcccgggatg ttttatcttc cagcatcagg gtgacagcct gccggagatc ggccagttcc
     3001 ttttgcattg ccagaagctg ttcctgtggg aaattttttt gtgtattttc acggtgtgaa
     3061 atgccgggaa tttgtttctc agggatattc acatttccgt atacacggat cagttctgac
     3121 acatccacga cagggttgtt tttcccgtca cgtgacacgg taacctttcc ctgttttaca
     3181 tgattgtata gcgttcttct ggtaatgccc gcggcttgtg ctgcctgtga aaggttgagc
     3241 aatgttttcg ccactgtata cccctgattt tatcgggtgt gaaaggtgtg taatttctca
     3301 cggtaaatgt atcgctgaat tttacccgtt tttccgttca ggatgtggtt ttctgacgcg
     3361 ctgcgcttgt caaaccggcg acggccacgc aatgaattgc gcccccgtca tcggttatta
     3421 gaggctgtct gcccccggat acccggattc tttctgatgc ctgacggtga cgatcacaat
     3481 ctggtccatt tcccggtcgt ggcggtagag catcacatag ccactgtcgc caaacccgat
     3541 caccagttcc ttatattcca gcggcagaaa cggcaccggg cggccaatgt ctggcaatgt
     3601 tttcagttgc tggatagccc ggacgatcac ctcacctcct tttctggctg ccagccgatt
     3661 tttggttttg agaaagtcct gaaggcgttg taaatcgcgc tgtgccagcg cggaaatcgt
     3721 tacctgtggc atggcggagc atcctgctcg ttctcagttc cccaggtact tatccaggct
     3781 tccgcttctt ccgcagtaag gtgcagaccc gtttcctgat agtgctgcca tgcggcctgt
     3841 ccgtcccgca ggtactggct gcgcttctct tcccggtcga tgtattcggt gattgcctcc
     3901 agcattagcg cgtgcgctga gcggtgccgg tcatcggcca gcgttttcag ccggtcctta
     3961 agttcttcat cgagcctgat ggatgttgct gatgacataa aagccccttt gtagtcatat
     4021 gtattacaca tgactacttt aatccgtggc ggcgctattt gccagaaata cggcttttgc
     4081 tgcccatttt tcagcttcgc ttccactaat cagggctgct acaggctcac tcatcatcca
     4141 ggactattgc agcctgcggg ttaaaggacc tgttactggc ggcaaccatc atcattgagc
     4201 ttttgggtgg tgactggtcg cggatcctgt aagccgtcat ttttcagact gaattcaaaa
     4261 tttataacct gtcgcattgc cgggtgccct gcgggatata gccagtgcct cattgccgtc
     4321 agcgtttccc gaaaaaaaag gtgacagccg gtaagcgaca cgttgtccac tcgtccatcg
     4381 tccagaatat caaacgtggc ggtcacctga ccttcgatgc gttttgctgc ggcctgtacc
     4441 gggtaggttg gctgcagtgt ggttacgggt tttagtggta tcgcatgtac cggagtgagg
     4501 aacatggccg ccagcactgt cagagtaaaa gctttcatgg tctgaaatcc agaggggcgt
     4561 ttttgaatat ttgccaggta ttttcatccg gtgccggcgg aattttggcg tgggtaacgg
     4621 ctgatattgc tgctttacag agtttcggat ctcctccttc agcaatggcg ctgttcagag
     4681 tgccatcacg ttgtatggat atccgcacgg tgcacatttt tcccgcatag gcctctggtt
     4741 caaacatctg catttcaaca gcggtcttga tggccgcagc gtagctactg attttagatg
     4801 acttatccag agtataaatt ttcggcggct gggtggtgga atgcttacag gccgtcagga
     4861 gtaaggggct gcacaatgcc agcaggataa agggtattaa acgcatcggg ttgatgacct
     4921 gtcagtaaaa aatgttacat gtatatttgt cactctttac ggccacgttc atttacataa
     4981 acacggctta tcgtatcgtt tttacaccaa acggcccgcc atgcagttgc gtgcataggc
     5041 atgcagcggg ttacaattta ccgggcgcgt ttccggcggt tttccgggtg gtttgttgcc
     5101 tgttttatcc cgtagccgcc ggaaacgccc tcagaccgtc tgagcggtgc gggcaatgcg
     5161 acgttatggt aaatacccct taccggcggc gctgtggctg ccaggtgaag gtggtttccg
     5221 tagcctcgta tttctcccga cgcatttcct gcgcccgcct ggcttcacgt tcgtcctgaa
     5281 tggcacgaag caccctttcc cgcacctgtt gttttaccgc ctttggcagc gttttgttcc
     5341 ctttcaggcc catggccagt gcctgagcga caaaggcgtc cgccaggcgc ttttcttcag
     5401 cgggagtcag tttcaggtgc gtcatagtgg cctccgggcg tctcaggttt cccgaacagt
     5461 ttaaccgcag gtcctctcct catgccagtt agctttcaga tgtgtctata ccatctgaaa
     5521 tgtaagtgtg atgaatgtct taaataataa aatttgttgc catgagaaat tttcgggaaa
     5581 agacgcgatc tgcttacagg tggctccggg cgcttttttg tgcctgaaga tgagaactaa
     5641 tctcatatat tgacacctga aaacgggagg agtaaggtaa taattatact gtgtatatgt
     5701 acagtgtgcg ccgggagacc ggttagagat caaggggtga aagtccccga ccattgaagg
     5761 accagcaatc cacaaggtcc ccgagtcatg cgttgcatac cgcgaggtat ggggcgaagc
     5821 gttgacaggg gtgttgacag gccagccatt gagccacgaa atgtatatta aattaccggg
     5881 tgccgacgtt gtactgttaa cggaaggcaa catcataggg tgcgacactg cgagtgccac
     5941 atggacccgg cggggtctga gaccctggca tgtcaatacg atctctacgc gggaaccggg
     6001 agatctcccc tctgaccatc tgccagtgtc ggagatggcc cgcaccggga agacgaggag
     6061 tcatagccgg tgatgtacgg agaggagaag tcggactcgc tcatagtagc ggcgaagcag
     6121 gcgaacaacc cgaaaggagc ggagtcagtg gagcgaagga gcggggccaa ggggaacgcg
     6181 gaacagccac acatgcgccg gacacagagc cgggaaagca tgtcacagag gctgtcacgc
     6241 gtgcgggaag ctgcgaagca gcggaagaaa gaacggttta cagcattgtt ccacctgctg
     6301 acagtcgaag cactggaagc cgcattcctc tccctgagca ggaaagcggc cgccggagtg
     6361 gatggcatca ggtggatgga ctacgccgga aacatgaaga acaacataac agatctgcac
     6421 cggaggctac atcagggcag ctacagggcg cagcccggca ggcgtcacta catcccaaaa
     6481 gcggatggaa aacaacgccc gctcggcatc gcctcgctgg aggacaagat cgtccagtat
     6541 gcgctggtga aaatcctgaa cgcagtctat gaaaacgact ttatggggtt ctcatacggg
     6601 ttcagacccg ggcgaagcca gcacgatgca ctggacgcac tggccacagg gctggtacgc
     6661 actaacgtaa actgggtact ggatgccgac atcagtcagt tcttcgacag ggtgagccac
     6721 gaatggctga tcaggttcac agagcatcgg atcggcgacc ggagggtaat caggctcata
     6781 cgtaagtggc tcacagccgg gacgtcggag gagggtcaat ggcgagcaac ggaggaaggc
     6841 accccacagg gtgcggtcat ctcaccgctg ctggcaaaca tatacctcca ctacgtcttc
     6901 gatctgtggg cgcatcagtg gcgacgtcgc tatgccacag gcaatgtggt aatggtcaga
     6961 tacgccgatg acatcgtcat cgggttcgac aaacgatacg atgcccggcg cttccgtata
     7021 gccatgcagc gcagactgag ggagttcgga ctcacggttc acccggagaa aacccgtctg
     7081 atggagttcg gccgcttcgc tgccgaaaac cgtgccatca ggggaaaagg caaaccagaa
     7141 acgttcaact tcctcgggtt cacgcacatc agcgggaaag atcgcaacgg caggttcatg
     7201 ctgatacgaa agacccgccg ggatcggatg acggcaactc tgaaagccat caaagacggt
     7261 ctgcgaaggc gctggcatta ctcaatcccc gaacagggaa aatggctcag gagagtggtt
     7321 cagggatacc tgaactatca ctcggtaccg ggcaacttcc ccaccatgca gaagttcagg
     7381 acacacgtaa caaacctctg gcgccgggcg ctcaggcgca ggagccagaa ggatgatacg
     7441 acctggacga aagcaaacaa actggcagcc gcatggctac caagggttcg ggttcttcat
     7501 ccatggcctg tggagcggtt caccgccaga cacccgaggc aggagcccgg tgcgtaaatc
     7561 gcgcacgccg ggatctgtgc ggggggtatc cggtaacggg tatccctacc gcgacatgtt
     7621 ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt ggagacggtc
     7681 gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg aatgtgaacg
     7741 gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac agcgggccgg
     7801 gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg ggggagtttg
     7861 gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat agcggtagct
     7921 ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc ctggctactc
     7981 cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc gccgttgctg
     8041 atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt gcgatctacg
     8101 gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt
     8161 cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg gaccaggcga
     8221 cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag catattgcgg
     8281 ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca aaacgtccgg
     8341 gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta ccgaaaggtg
     8401 ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg
     8461 ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg aaagagaccg
     8521 gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag gtaaaacagc
     8581 gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg gaagaggggc
     8641 tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa aatattgcga
     8701 ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg gctgctgttc
     8761 aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt gtgacttata
     8821 acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt gataaggata
     8881 ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca atgaatgaat
     8941 ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa caaaaggaga
     9001 aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct cgcaagggga
     9061 ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata aagggacttg
     9121 gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt aagcgggccc
     9181 gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac ggtgagcttg
     9241 aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa acgggtaagc
     9301 aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag gtaaatatgg
     9361 ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa ggggaagagt
     9421 attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg cctttaaagg
     9481 ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta ctacagccat
     9541 tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt gattatcggg
     9601 acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt ccggtgttca
     9661 gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta aagactattt
     9721 ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca aattatatcc
     9781 gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg
     9841 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg
     9901 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg
     9961 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag
    10021 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc
    10081 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgtct ttcccgggtt
    10141 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg
    10201 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta
    10261 tgagaaagcg ccacacttcc cgcaagggag aacggcggac aggtatccgg taaacggcag
    10321 ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg atctttaagt
    10381 cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg
    10441 caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg
    10501 ccaccggcgc ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc
    10561 tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg
    10621 accgagtgag cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct
    10681 gatgtgggat attgtcccct gattaagtct ttctattccc tgacgatcaa ttatcaggat
    10741 aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt
    10801 tctccttgcg gagaaatgca agtcatcccc gctggggcgt ggctgacttc aggggaaaag
    10861 accttgctta cgctggctgg acgccaccga cgcacggtct tttcccctct ttctggctct
    10921 gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga
    10981 ccacccacaa aaatccacgg tgctggacgc acctgacgaa gcagcaaagc tgctcctgat
    11041 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
    11101 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
    11161 gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgcggtga aagctgaacg
    11221 caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg gcttgtgcag
    11281 cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa
    11341 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
    11401 ggtcagctgg ctgtaccggg agtattacag tctggccggt ttttttctgg ttatcggcct
    11461 gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt tttgtggggc tgagccataa
    11521 ggcgcggcgg gagtatgccg atcgctataa gcgcgataat aaccggactg tgccattttc
    11581 ggtgctggcc tcgctggatt cacccgggcg gatggtcagg gcacggcgac atgtctggaa
    11641 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
    11701 cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga
    11761 ttattacctg ctgcagccgg ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt
    11821 cgccggcgtc cttctggcgc ggctgctggc gttcctgctg cttgatggtc attttgcggc
    11881 cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
    11941 cggagaaatg acagacgtcc gccacctgca t
//
