LOCUS       NZ_CP127006            10648 bp    DNA     circular CON 28-OCT-2023
DEFINITION  Escherichia coli strain YZ3-11 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP127006
VERSION     NZ_CP127006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35565424
            Assembly: GCF_033037735.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Wang,Y.
  TITLE     Novel one-tube RPA-CRISPR-Cas12b-based detection systems for
            sensitive and specific monitoring of mcr-1 and tet(X4)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   Wang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2023) College of Bioscience and Technology,
            Yangzhou University, 48 wen hui road, Yangzhou 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2023
            Assembly Method                   :: flye v. v2.8.3
            Assembly Name                     :: E. fergusonii-mcr-1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 142.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/28/2023 14:05:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,887
            CDSs (total)                      :: 4,767
            Genes (coding)                    :: 4,633
            CDSs (with protein)               :: 4,633
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 56 of 134
            Pseudo Genes (incomplete)         :: 83 of 134
            Pseudo Genes (internal stop)      :: 22 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            Pseudo Genes (short protein)      :: 2 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP127006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="YZ3-11"
                     /isolation_source="pork"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="China: Yangzhou"
                     /collection_date="2023-03"
     gene            complement(join(10214..10648,1..89))
                     /gene="cri"
                     /locus_tag="QQC31_RS23930"
                     /old_locus_tag="QQC31_23865"
                     /pseudo=""
     CDS             complement(join(10214..10648,1..89))
                     /gene="cri"
                     /locus_tag="QQC31_RS23930"
                     /old_locus_tag="QQC31_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /name="cri"
     gene            complement(247..1475)
                     /locus_tag="QQC31_RS23935"
                     /old_locus_tag="QQC31_23870"
     CDS             complement(join(247..1160,1160..1475))
                     /locus_tag="QQC31_RS23935"
                     /old_locus_tag="QQC31_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_317579512.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREVLHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRASN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            1569..1685
                     /locus_tag="QQC31_RS23940"
                     /old_locus_tag="QQC31_23875"
     CDS             1569..1685
                     /locus_tag="QQC31_RS23940"
                     /old_locus_tag="QQC31_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_317579513.1"
                     /translation="MLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1903..2016)
                     /locus_tag="QQC31_RS23945"
                     /old_locus_tag="QQC31_23880"
     CDS             complement(1903..2016)
                     /locus_tag="QQC31_RS23945"
                     /old_locus_tag="QQC31_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071699851.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2686..2877)
                     /locus_tag="QQC31_RS23950"
                     /old_locus_tag="QQC31_23885"
     CDS             complement(2686..2877)
                     /locus_tag="QQC31_RS23950"
                     /old_locus_tag="QQC31_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2942..3265
                     /locus_tag="QQC31_RS23955"
                     /old_locus_tag="QQC31_23890"
     CDS             2942..3265
                     /locus_tag="QQC31_RS23955"
                     /old_locus_tag="QQC31_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_040090312.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPLARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3474..>3905
                     /locus_tag="QQC31_RS23960"
                     /old_locus_tag="QQC31_23895"
                     /pseudo=""
     CDS             3474..>3905
                     /locus_tag="QQC31_RS23960"
                     /old_locus_tag="QQC31_23895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3943..4473
                     /locus_tag="QQC31_RS23965"
                     /old_locus_tag="QQC31_23900"
     CDS             3943..4473
                     /locus_tag="QQC31_RS23965"
                     /old_locus_tag="QQC31_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_073534392.1"
                     /translation="MSNLLQTGAEFEKKLKERAASTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFTTTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DKGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            4480..4713
                     /locus_tag="QQC31_RS23970"
                     /old_locus_tag="QQC31_23905"
     CDS             4480..4713
                     /locus_tag="QQC31_RS23970"
                     /old_locus_tag="QQC31_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_073534391.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRGNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5452..6149
                     /locus_tag="QQC31_RS23975"
                     /old_locus_tag="QQC31_23910"
     CDS             join(5452..5701,5701..6149)
                     /locus_tag="QQC31_RS23975"
                     /old_locus_tag="QQC31_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(7134..7553)
                     /locus_tag="QQC31_RS23980"
                     /old_locus_tag="QQC31_23915"
     CDS             complement(7134..7553)
                     /locus_tag="QQC31_RS23980"
                     /old_locus_tag="QQC31_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_239170938.1"
                     /translation="MRLTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPIKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7634..8071)
                     /locus_tag="QQC31_RS23985"
                     /old_locus_tag="QQC31_23920"
     CDS             complement(7634..8071)
                     /locus_tag="QQC31_RS23985"
                     /old_locus_tag="QQC31_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_169750488.1"
                     /translation="MGHCNFMAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            8297..10186
                     /gene="cra"
                     /locus_tag="QQC31_RS23990"
                     /old_locus_tag="QQC31_23925"
     CDS             8297..10186
                     /gene="cra"
                     /locus_tag="QQC31_RS23990"
                     /old_locus_tag="QQC31_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="WP_317579514.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDCWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDDFVDKLNNEIIRPAY"
                     /name="cra"
     Typing          2514..2601
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.506"
                     /coverage="100.0"
                     /evalue="3.21e-32"
     Typing          complement(1945..2491)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.264"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3256..4796
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.393"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaataataa ttaatgggat tatacccgcg aaagtaatca aaaaaagcat cctgtttaaa
       61 tctttgttgt aagtgtactc tttttgcatt ggtataatac tcccggtttt tcattgttat
      121 taatttagtt gttgatgtgt ataagcatat catgctgtgc gagtaatggt caccctaata
      181 ttaggggtat gagttaatga ggggatatat gaaaatgata ttatcaggag ggatgtggat
      241 ttgcccctat atttccagac acctgttatc acttaaccca ttactggccc gctgccgcag
      301 atattcccgt ggcgagcgat aacccagcgc actatgcgga tgccattcgt tataatgctc
      361 gaacgcctct gcaaggttct ttgctgccgt taacccgtct ggtttgggca tgatactgat
      421 gtagtcacgc tttatcgttt tcacgaagct ctctgctatt ccgttactct ccggactccg
      481 caccgccgtg ttcttcggtt caagtcccaa catccgggcg aactggcgtg tttcattagc
      541 ccggtagcat gaaccattat ccgtcagcca ctccactgga gacgacggaa gatcgttgcc
      601 gaagcggcgt tccaccgctc ccagcatgac gtcctgtact gtttcactgt tgaagccgcc
      661 ggtagtgact gcccagtgca gtacctcacg atcacagcag tccagtgcga acgtgacacg
      721 cagtctctct ccgttatcac agcagaactc gaacccgtca gagcaccatc gctgattgct
      781 ttctttcacg gccactctgc ctgtatgtgc ccgtttcgat ggcggtacag caggttttcg
      841 ctcaagcaac agcgcattct ggcgcatgat ccggtaaaca cgtttggcat tgatcgcagg
      901 cataccatca agttctgcct gtctgcgaag cagcgcccat acccgacgat aaccatacgt
      961 gggcagctct ccgataacat ggtgtatacg gagaagcaca tccgtatcat cagtgtgacg
     1021 actgcggcgg ccatccatcc agtcatcggt tcgtctgaga atgacgtgca actgcgcacg
     1081 cgacacccgg agacaacggc tgactaagct tactccccat ccccgggcaa taagggcgcg
     1141 tgcgctatcc acttttttgc ccgtccatat tcaacggctt ctttgaggag ttcattttcc
     1201 atcgttttct tgccgagcag gcgctggagt tctttaatct gcttcatggc ggcagcaagt
     1261 tcagaggcag gaacaacctg ttctccggcg gcgacagcag taagacttcc ttcctggtat
     1321 tgcttacgcc agagaaataa ctggctggct gctacaccat gttgccgggc aacgagggag
     1381 accgtcatcc ccggttcaaa gctctgctga acaattgcga tcttttcctg tgtggtacgc
     1441 cgtctgcgtt tctccggccc taagacatca atcatctgtt ctccaatgac tagtctaaaa
     1501 actagtatta agactatcac ttatttaagt gatactggtt gtctggagat tcagggggcc
     1561 agtctaggat gcttgtcttg atggctttgc tatcaggctg ccaggctaat tatattcgcg
     1621 atgttcaggg agggaccgtc gcaccatcct cctcttctga actgacggga atcgcggttc
     1681 agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1741 gcaaacaaaa aaaccaccgc taccaacggt ggtttgtttg ccggatcaag agctaccaac
     1801 tctttttccg aaggtaactg gcttggagga gcacagtcac caaaacctgt cttttcagtt
     1861 tagccttaac cggctcataa cttcaagacc aactcctcta aatcagttac cagtggctgc
     1921 tgccagtggc gctttgtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2041 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2101 gagaaaggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc gcacgaggga
     2161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg
     2281 cgaggccttt cgttgccctt tgcctttttg ctcacatgtt ctttccggcc ttatcccctg
     2341 attctgtgga taaccgtatt actgcctttg agtgagctgg caccgctcgc cgcagccgaa
     2401 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     2461 tccttacgca tctgtgcggt atttcacacc gtatatggcg ctgttttctt acaatccgca
     2521 ctgatgctgc atatctaagc cagtatacac tccgctatcg ctatgtgact gggtcagggc
     2581 tgcgccccga cacccgctaa aacctgctga cgcgccctga cgggcttgtc tgctcccggc
     2641 atccgctcac agacaagctg tgtccgtctc cgggagctgc atatgtcaga ggttttcacc
     2701 gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt cgtgaagcga
     2761 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttttcca gcagcgttaa
     2821 tgtctggctt ctgataaagc gcgccatgtt aagggcggtt ttttcctgtt tagtcactga
     2881 tgcctccgtg taagggggga tttctgttca tggggtaatg ataccgatga aacgcgagag
     2941 gatgctcaca atacgggtta ctgatgatga acatgcccgg ttacttgaac gctgtgaggg
     3001 taaacaactg gcggtatgga tgcggcgggt ctgcctgggg gagccgcttg cccgttccgg
     3061 gaaactgccg acactggcac cgccgttact gcgtcagctg gccgccatcg gaaataacct
     3121 gaatcagaca gcccgtaagg tgaacagcgg gcagtggtct tccggtgatc gggttcaggt
     3181 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     3241 acagggggcg cgggatgata gttaaatttc atgccagggg aaaaggtggt ggcagtggtc
     3301 cggttgatta cctgctgggg agggagcgta accgcgaagg cgcaacggtg cttcagggta
     3361 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtcag
     3421 gtgttctgtc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     3481 gctttgagcg tgtactgatg cccggtctcg aaaagaatca gtacagcatc ctgtgggtag
     3541 agcaccagga caagggacgg cttgagctga attttgtcat tccgaacatg gagttacaga
     3601 ccggaaaacg cctccagccg tactacgacc gggcagacag gccgagaatt gatgcctggc
     3661 agacgctggt taatcaccat tacgggctgc atgaccccaa cgccccggag aaccgccgga
     3721 tactgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag ggcgtcacac
     3781 gaggtataga tgcactttac catgccggag agataaaagg tcgtcaggat gtgattcagg
     3841 cgctcacgga agcggggctt gaagtggtca gagtgacgcg aagcagcatc agcattgcag
     3901 acccgaacgg cgggaagaat atcaggctga aaggagcatt ctatgagcaa tcttttacag
     3961 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg catctacaga gaaaatgctg
     4021 aacgacgagt tcaggaagct cgaagaatct gtaaacaggg aactgacctc aaacgaatct
     4081 ttaatcagga acgctatcaa cgaccataca acagccctga aagaacttct ggagaggtat
     4141 cagaaaacga ccgtcagtac gatggacaca cactggcaaa ccgtgctgaa aatgagcgtg
     4201 aaacgctggc tgtggctgat tatcgtctca gtgctgatgt ttaccacgac cgggagtctg
     4261 ctgtggtacc agggcatgaa gataaacgca aacatgaata ttctccgcga gcagaaagag
     4321 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgagga cagcaacgga
     4381 cgttttctgg tgttgccgaa ggggatgaaa gccgaaacga actggacgga ggacaaaggc
     4441 aaactgaacg cagtgagact ggtacaggag tgacagagca tgacggagct ggaaaaacat
     4501 ttgctgaacg cattagagca actgcaacag gactatatgc agcggctgaa cgaatgggag
     4561 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacggggcaa cgcgatgctg
     4621 aacgagcggg tcatgcagtt gagtcagcag gtgcagcact tgagcgagcg gacagagcgc
     4681 ttgagccagt tatacagcga gaacaggaga taagggatga acggctgatg caggaacgcg
     4741 aacatgagtt atctctggaa cgggagcgga cgctggatgg cccttcgctg ggatggtgac
     4801 tgaaagtccg gttgagtcgt aaacagaata ttttccagaa atgtcaggct gcctattggt
     4861 aatataacca gtcgttcttg cggtttattg atttttgtac aggttgttgc tgtttttttt
     4921 tgtaaagagg acagggattt ttctgaaaat gaacttgcgg gaatgcgctg attatgtaaa
     4981 aaatataatt gcgtatctcc gatatgtttg taataccgat gtggacacag cgaatctgga
     5041 aggggtcgga gatgcgcgtt ctctcatgtg ttaatattgt gttcttatat tgtgatgtga
     5101 atatttttta ttttttcttt gcatcttgga aacatgcaaa ttatattctg atatgcaaaa
     5161 gtcaggacta gacatactat ccacctattc agttcttacc ttacgccctc actaagttcc
     5221 tgccgtgagg gcttttttca tgggtgtgaa aaaaggaagc cccacctgac ggaggggctg
     5281 agtgatacag agcaaaaaaa tatcctggtg ttggtcacct ctggagagta ataagattaa
     5341 ccataaagcg aactataaac aagaatattg atgccaggaa ttttctgggt taattgggtg
     5401 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     5461 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     5521 accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc
     5581 acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc
     5641 gttggatgcc gggcaaccgc ccgcattatg ggcgttggcc tcaacacgat tttccgccat
     5701 ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg
     5761 tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc cagcgctggc
     5821 tgttttacgc gtatgacagg ctccggaaga cggttgttgc gcacgtattc ggtgaacgca
     5881 ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga
     5941 tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca
     6001 agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc
     6061 tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc
     6121 attatctgaa cataaaacac tatcaataag ttggagtcat taccggttaa ttgttttgtt
     6181 ttttacaaaa aagacaaaat aacagaaaat aaaatctaat catttttgtt gatcgttgtt
     6241 gctcggtgcc gttattctta agctgaaagt gaattcttct aattttttct tatattcaga
     6301 cgcaattaac agcccctgct ccttgaggga ctttttttta tctttttctc tctgttttat
     6361 actgttttga gtagtaacta taaaggcagt gaagttctgt cttatgagcg catttctggc
     6421 ggtgatgcga ctaacccatt ctacatcaac gaacaattca ccagaaacgc gcctgtaaat
     6481 ttttagctga gcaagggctc tgtgtcgaga ggtgtggatg tctggttcac cattttaaaa
     6541 cacttcttcc ggcggttttt agtttcgatg caggccaggg ggcgctgtaa agttatggtc
     6601 cctgattact gtgccagaat tctgagacag acaggaggtc taccggttat ttttcgaatt
     6661 gggcgcttcc ggaagagaga gtttcattgg acgttggtcg cggatggtga gcacgtttcg
     6721 atgttgtgtg acgaacattc agctcagttg atggagactg tcagaaacgc gctcagaagt
     6781 gttgtaacgc gatttctggt atcaggcaat tgtttccttt ttctggatcc ttatacagtg
     6841 tgtgtacggt gatgagttct gtcacgcaca gtaagattcc cccccccaga tgcttccatc
     6901 tggcaggaat tgatccggct gaaaatatga aaaaacaccc gtttaagtaa tctcaggcag
     6961 gtaattcgtt cgtgattcgc ctggctgcat agatatgcat aagattaaaa tttatcaggc
     7021 gcgatcgcgg cggtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgagatc
     7081 gcgctgaacg catttcagtg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     7141 cttccctcgt agccatcgag ataaaccgca gaaatcgtct cagccagcag cctggatttt
     7201 atcggggtaa ggtctttgct gcggtaatca gtacccgcaa attgagttgt gccccggagt
     7261 gagtttaaaa attcttggct gtgaaactgc tgaatgcgtt gttgcacatc actccgtgat
     7321 tttcctgtcg ctttatcttt agctcccata tcccagaact gccgaaaaaa aggcaccatc
     7381 attcgtgttg tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc atcagaagca
     7441 tatacgtaat gccgggtatg ttgttccacg ctgccctttg gggtacaggc tgtgagagct
     7501 actgttgctg ttatcatggc gataagacaa agtttgtttc cggtaagtct catgagtgtc
     7561 tccctacctt aaagtattta ttctcggtga gttattatcc gaagactatt ttatcatcag
     7621 tctctgacag cgttcagcga ccgaacaacc tggaccagaa tccccgtttc ttttccagtt
     7681 ccagtgcctg cctgagctgg gctatctcat tttgtagctg ttcccgttct gcttcctggt
     7741 gtctgcgatc catatcctgt gcctgtttat cctcaagcat cagcgtcagg cattgtttca
     7801 gctcattcag ttcccggaga atgcgttctg tctgctgatc atgtggattt tctgcctgtc
     7861 ccacagtgtg cctttcttgt gtctcatttt gctttaattc gccgtatgcc cggatcagtt
     7921 cactggtttc aaactccctt cgtccgtcct ggctggtacg gtaactgaca agacctgagc
     7981 gcatatcacg gtaaaactgt gaacgcgatc ttccagtcat tttcagtgcg tcctggagtg
     8041 tgtgccatgc cataaagtta cagtgtccca taggtgtctc atctcatagt ttcagtaaaa
     8101 cataatgagg tctgagaacg gtaatgtttg cgccggtttt tgtggtttcc ggcgggaata
     8161 gcgcgtggtg tgagagactg tttttttgat cgttttcaca aaaatggaag tccacagtct
     8221 tgacagggaa aatgcagcgg cgtagctttt atactgtaca taaaaccagt ggttatgtgt
     8281 acagtatttg tttttaatgg tttgttttaa aagtcaaaga ggaatgaata tgccggggtt
     8341 taattacggt gggtatggtg acggtactgg ctggagttct gaaagtggtg gtccagctcc
     8401 aggcggtggt atgcatggaa atagtggagg ccagcgcgga gataacgcaa acagtagcaa
     8461 ttctgtgagt cagcagattt ctgccattca gaatgatcag aaacttaaac agaaagtggt
     8521 gaatatgctg attgctgcga gaaaaatgaa tcctgaagca aaaatgattc tgggaagtat
     8581 tgctccttct ggtgttatgc aggtcaccat tgagggggta acctccactc aggccaggca
     8641 gcttggtttg ggagggctgg tcatgggata taacgcttct ggtgttattg gggctgtggg
     8701 cgaaatagac accggtcacc gccttaatgc atcaggagca agtactcccg gaagcgaaac
     8761 ttctgtggag agttttgtga acggacagaa accagccgga gaatggcatg ctgtggcgaa
     8821 ggactgctgg acaggggctg gtccagtgaa tgtagggctg gttaataacg ccatcaaaag
     8881 tgtccggatt atcaaaaaag gctatgtgac gggcgttctc acgccagaag aggtgatgaa
     8941 taaagcagag tacaaagcaa tgcggcaggc tttcgattcc cttccactgg caaaacaggg
     9001 agaggctgtc agacagattg ttgctgcttg gagtctggca tatcaggatt ttccggtaaa
     9061 tctgaaaaaa gagatggggc gtgtcactga acggattgtt gatgctatta acctagcgct
     9121 cattcttaat cagacagagt cccgtttgtc ggaatcacaa aagaatgttg atgtagcgaa
     9181 ccagattatc agtgatacag taaaagctat taatgatgta aataagaaaa tagcagaaaa
     9241 acgaaatcag caggtatcac tgactgattt gatgaataaa aaacaaaaag aggtcgaaga
     9301 tcttaaaaag atattcaaaa atcactctta tcaccgtatt cgtgatgccc agagggaata
     9361 tgatgatgcc cgaaataaat atgctttact ggccagtgat attaatgcgc tgcaggcaca
     9421 ggtttccgga ctgacggcaa ggaaacagca ggcagaacag aacaaggcag cagcagaaaa
     9481 agcaaaggca gatgcagcgg cgaaagctgc agcagaaaaa gcggctgctg aagcgaaagc
     9541 aaaggctgaa gctgaaaaag ccagaaagga agcagaggaa aaagcaaatg acgagaaggc
     9601 tgttctgaca aaagccagtg aaattatcat cagtgttggt gataaggctg gggagtatct
     9661 gggtgataaa tataaagttc tttctcgtga gatagcagat aatatcaaaa actttcaggg
     9721 caaaacgatt cgtagttatg atgaggcgat ggcttctgtc aataaactga tggctaatcc
     9781 tgatcttaaa attaatgccg ccgacaggga tgcaattgtg aatgcctgga aagcatttga
     9841 tgcagaggat atggggaata agtttgccgc gctgggtaag acgtttaaag ccgcagatta
     9901 tgtgatgaag gcaaacaacg tccgggagaa gagtatcgag gggtatcaga ccggaaactg
     9961 ggggccgctg atgctggaga ttgagtcctg ggtattgagt ggtattgctt ctgctgtggc
    10021 gctatcattt ttctcagtcg ctgttggttc attatttatt acagcgggcg tgtctgtaac
    10081 ggctgtggga cttatgggca ttatttttgc tggctttatc ggtgcgctga ttgatgatga
    10141 ttttgttgat aagttaaata atgaaattat tcgccctgcg tattaattga gagtcgaaag
    10201 gtagtcagga ggactacctt tctttccaga gtttatatgc tattactggt tggaggcaga
    10261 gggcgtaagt tatgaaaaaa gagcaataat acaggcagat ataaaagata agcaaagtaa
    10321 gatcgttctc agacatcaat cgaacaggtc ttcccgatgt tgttaactcg aagttatgcc
    10381 acaagaaaaa ataagcataa aaaatacaca aaaaggggga cagtatacaa gctgttatga
    10441 ttttttttct gtctgttatt ttgggggttc ttgtgtgttt gaatagcatg atgaaagcta
    10501 taaatgctaa taatggtgcg ctcttaccgt agatatccat cacctttgtc atcacagggt
    10561 tatatgaaga aattactgaa ggtatatttt gagtattatc gaaaatgatg cggattaaat
    10621 agaagttggg gtttgatgta taagttat
//
