LOCUS       NZ_CP126991            10060 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain T3 plasmid pT3_p4,
            complete sequence.
ACCESSION   NZ_CP126991
VERSION     NZ_CP126991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35562460
            Assembly: GCF_030252445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhou,A.
  TITLE     Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) strains isolated
            from Shanghai East Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhou,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2023) Medical Laboratory, Shanghai East Hospital,
            Yuntai Road 1800, Shanghai, Shanghai 200123, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030252445.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 04:48:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,476
            CDSs (total)                      :: 5,354
            Genes (coding)                    :: 5,229
            CDSs (with protein)               :: 5,229
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 48 of 125
            Pseudo Genes (incomplete)         :: 75 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 14 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126991.1.
            Bacteria and source DNA available from Shanghai East Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T3"
                     /isolation_source="Shanghai East Hospital"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pT3_p4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.11 N 121.29 E"
                     /collection_date="2021"
                     /collected_by="Shangai East Hospital"
     gene            join(9316..10060,1..941)
                     /locus_tag="QP573_RS27245"
                     /old_locus_tag="QP573_27245"
     CDS             join(9316..10060,1..941)
                     /locus_tag="QP573_RS27245"
                     /old_locus_tag="QP573_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            951..1208
                     /locus_tag="QP573_RS27250"
                     /old_locus_tag="QP573_27250"
     CDS             951..1208
                     /locus_tag="QP573_RS27250"
                     /old_locus_tag="QP573_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1293..1442
                     /locus_tag="QP573_RS27255"
                     /old_locus_tag="QP573_27255"
     CDS             1293..1442
                     /locus_tag="QP573_RS27255"
                     /old_locus_tag="QP573_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2926..4890
                     /locus_tag="QP573_RS27260"
                     /old_locus_tag="QP573_27260"
     CDS             2926..4890
                     /locus_tag="QP573_RS27260"
                     /old_locus_tag="QP573_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4890..5621
                     /gene="mobC"
                     /locus_tag="QP573_RS27265"
                     /old_locus_tag="QP573_27265"
     CDS             4890..5621
                     /gene="mobC"
                     /locus_tag="QP573_RS27265"
                     /old_locus_tag="QP573_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5628..6158
                     /locus_tag="QP573_RS27270"
                     /old_locus_tag="QP573_27270"
     CDS             5628..6158
                     /locus_tag="QP573_RS27270"
                     /old_locus_tag="QP573_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6185..6364)
                     /locus_tag="QP573_RS27275"
                     /old_locus_tag="QP573_27275"
     CDS             complement(6185..6364)
                     /locus_tag="QP573_RS27275"
                     /old_locus_tag="QP573_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6390..6818)
                     /locus_tag="QP573_RS27280"
                     /old_locus_tag="QP573_27280"
     CDS             complement(6390..6818)
                     /locus_tag="QP573_RS27280"
                     /old_locus_tag="QP573_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6982..7296)
                     /locus_tag="QP573_RS27285"
                     /old_locus_tag="QP573_27285"
     CDS             complement(6982..7296)
                     /locus_tag="QP573_RS27285"
                     /old_locus_tag="QP573_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7284..7562)
                     /locus_tag="QP573_RS27290"
                     /old_locus_tag="QP573_27290"
     CDS             complement(7284..7562)
                     /locus_tag="QP573_RS27290"
                     /old_locus_tag="QP573_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7737..8102)
                     /locus_tag="QP573_RS27295"
                     /old_locus_tag="QP573_27295"
     CDS             complement(7737..8102)
                     /locus_tag="QP573_RS27295"
                     /old_locus_tag="QP573_27295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8099..8470)
                     /gene="tolA"
                     /locus_tag="QP573_RS27300"
                     /old_locus_tag="QP573_27300"
     CDS             complement(8099..8470)
                     /gene="tolA"
                     /locus_tag="QP573_RS27300"
                     /old_locus_tag="QP573_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8744..8989)
                     /locus_tag="QP573_RS27305"
                     /old_locus_tag="QP573_27305"
     CDS             complement(8744..8989)
                     /locus_tag="QP573_RS27305"
                     /old_locus_tag="QP573_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1546..2124)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4891..5618
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg tgattcacgt ccggctggtt
       61 ttacagccgg tggtaactcc cgtgaggccg ttattcgttt cccgaaagag accggacaga
      121 agccggttta tgtgtcggtg acagatgttc ttaccccggc acaggtaaaa cagcgtcagg
      181 aggaagaaaa gcgtcgccag caggcatggg acgccgctca tccggaagag gggctgaaaa
      241 gagactatga taaagcgaaa gctgagctgg atgccgaaga taaaaatatt gcgaccttaa
      301 acagccgcat tgcatcgaca gagaaggcgc tccccggtgc aagggctgct gttcaggaag
      361 ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga ttttgtgact tataacccgc
      421 ctcatgaata tggctccggg tggcaggatc aggttcgcta tcttgataag gatattcaga
      481 atcagaatga gaaattaaaa gcggctcagg catctttaaa cgcaatgaat gaatccttat
      541 ccagagataa ggctgcactt accggggcga tggagagccg gaaacaaaag gagaaaaaag
      601 cgaaggatgc agaaaataag ttaaatgagg aaaagaacaa gcctcgcaag gggactaaag
      661 actacggcca tgattatcat ccagtaccta aaacagaaga cataaaggga cttggagagt
      721 taaagcgagg tgatcctaaa acacctaaac aaggtggtgg tggtaagcgg gcccgatggt
      781 atggcgataa aaaacgtaaa atttatgaat gggattccca gcacggtgag cttgaagggt
      841 atcgtgccag tgatggcgaa cacctaggcg catttgatcc caaaacgggt aagcaggtta
      901 aagggccaga tccaaaacgg aacattaaaa aatatctttg agaggtaaat atggggctta
      961 aattacgttt gacatggttt gataagaaaa ctgaagagtt taaaggggaa gagtattcta
     1021 aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta aaggataata
     1081 ttaataatgg tgagtttgat ttgaagaaag aatgggttcc tttactacag ccatttttta
     1141 aaaatcaaat aaaacctgat gtggataatt gttatatagc ttttgattat cgggacggta
     1201 actggtaaca aaaaattatc aaggatgatt tgaaatactg acttccggtg ttcagggatg
     1261 aatgccggag tctttaaata cagggaaagt atatgaaaaa ggtaaagact atttttcttt
     1321 ttatattgat tgttgctggc tttcttctgg ccgcatgtca ggcaaattat atccgggatg
     1381 ttcagggcgg aacggtggca ccatcgtcct cctctgaact gacggggatc gcggttcagt
     1441 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct tttgccctgt
     1501 aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt ggggaactgc
     1561 ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc
     1621 cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa
     1681 ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg gttggactca
     1741 agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc ttgcttacag
     1801 cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa
     1861 gcgccacact tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg cagggtcgga
     1921 acaggagagc gcaagaggga gcgacctacc ggaaacggtg gggatcttta agtcctgtcg
     1981 ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag atgcaccgaa
     2041 aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa acgccaccgg
     2101 cgcggccctg ccgttttgcc tcacatgtta gtcccctgct tatccacgga atctgtgggt
     2161 aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt
     2221 gagcgaggag gcgcaatttc cccggcacct gcaagacggt caccggacgc gctgatgtgg
     2281 gatattgtcc cctgattaag tctttctatt ccctgacgat caattatcag gataggtgaa
     2341 ccatgtcggt ttttcgctga acattttacg ttaacctatc tctgttgctt ttttctcctt
     2401 gcggagaaat gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa aagaccttgc
     2461 ttacgctggc tggacgccac cgacgcacgg tcttttcccc tctttctggc tctgtccggc
     2521 tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca
     2581 caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg
     2641 gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca
     2701 ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc
     2761 gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac
     2821 cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc
     2881 tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat
     2941 tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc
     3001 tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc
     3061 ggtctggctg aactctgggc cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg
     3121 cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgtgccatt ttcggtgctg
     3181 gcctcgctgg attcacccgg gcggatggtc agggcacggc gacatgtctg gaactacgcg
     3241 tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt
     3301 atcgagagct ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc agattattac
     3361 ctgctgcagc cggctaacct gttcgggata gcctggtgtg ccggagggct tgtcgccggc
     3421 gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac
     3481 gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa
     3541 atgacagacg tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat
     3601 ttttccacgg agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc
     3661 cagccggtgc tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg
     3721 cccgggagcg gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc
     3781 ggggacgctg tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg
     3841 gcgcactgcc cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat
     3901 ctgttccgcg atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg
     3961 agtgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg
     4021 attgcggagc agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg
     4081 ttgccggagg ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc
     4141 gacctgagcg tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc
     4201 ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg
     4261 ctgtttgccc gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac
     4321 gcgagcatca tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg
     4381 gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc
     4441 ggtcagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg
     4501 ccgatccgct ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag
     4561 accggggaga tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg
     4621 gagcacatca gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac
     4681 accctgcaac acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg
     4741 gcgttcagca gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg
     4801 ctggcgaaaa cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa
     4861 gcggatgatg attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg
     4921 cgaagcggca ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag
     4981 agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg
     5041 tttaccgctt cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg
     5101 ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg
     5161 acgaggacga gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc
     5221 agctgccgca ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca
     5281 ccggctggcg gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg
     5341 cggagctgga cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc
     5401 ggtatcagga agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa
     5461 tctggtacat ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg
     5521 tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg
     5581 agaggctgtt cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca
     5641 tggtatttat tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg
     5701 agcatctgga agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg
     5761 tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca
     5821 acagcgacga aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca
     5881 aacccacagg aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg
     5941 agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga
     6001 ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc
     6061 tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca
     6121 aacccgcccg ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt
     6181 gctgttattc ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt
     6241 gaagccgttc gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca
     6301 acagcagcga ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg
     6361 tcatgttctc cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt
     6421 ttcttctgtg tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca
     6481 tcatcgtgct ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca
     6541 gcctgccgga gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt
     6601 ttttgtgtat tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt
     6661 ccgtatacac ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac
     6721 acggtaacct ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct
     6781 tgtgctgcct gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg
     6841 gtgtgaaagg tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg
     6901 ttcaggatgt ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa
     6961 ttgcgccccc gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg
     7021 atgcctgacg gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac
     7081 atagccactg tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac
     7141 cgggcggcca atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc
     7201 tccttttctg gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc
     7261 gcgctgtgcc agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca
     7321 gttccccagg tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc
     7381 tgatagtgct gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg
     7441 tcgatgtatt cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg
     7501 gccagcgttt tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac
     7561 ataaaagccc ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct
     7621 atttgccaga aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc
     7681 tgctacaggc tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac
     7741 tggcggcaac catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg
     7801 tcatttttca gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga
     7861 tatagccagt gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc
     7921 gacacgttgt ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg
     7981 atgcgttttg ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt
     8041 ggtatcgcat gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc
     8101 atggtctgaa atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg
     8161 gcggaatttt ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc
     8221 cttcagcaat ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca
     8281 tttttcccgc ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg
     8341 cagcgtagct actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg
     8401 tggaatgctt acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta
     8461 ttaaacgcat cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct
     8521 ttacggccac gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc
     8581 cgccatgcag ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg
     8641 cggttttccg ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac
     8701 cgtctgagcg gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg
     8761 gctgccaggt gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc
     8821 gcctggcttc acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta
     8881 ccgcctttgg cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg
     8941 cgtccgccag gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg
     9001 ggcgtctcag gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt
     9061 cagatgtgtc tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg
     9121 ttgccatgag aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt
     9181 tttgtgcctg aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag
     9241 gtaataatta tactgtgtat atgtacagta tgttggcttt aatttattgt tttaaatgtc
     9301 aaagaggaaa acggtatgag tggtggagac ggtcgaggtc cgggtaattc aggtctggga
     9361 cataatggtg gtcaggccag tgggaatgtg aacggtacgt ctggtaaagg tggcccttca
     9421 tcaggtggtg gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacgccgaat
     9481 ggagatattc ataactataa tccgggggag tttggtaacg gcgggagtaa acccggtggt
     9541 aatggtggta acagcggcaa tcatagcggt agctctggtg gcggacagtc ttcggccact
     9601 gcgatggcct tcggtctgcc tgccctggct actccggggg ctgaaggact ggctttatca
     9661 gtttccggcg atgcgttgtc ggccgccgtt gctgatgtgc tggctgccct gaaggggccg
     9721 tttaagtttg gtctgtgggg gattgcgatc tacggtgtgc tgccttctga gattgcaaaa
     9781 gatgatccga aaatgatgtc aaaaattatg acgtcattac cggccgatgc ggtgacggag
     9841 actccggcaa gtactttacc actggaccag gcgacggttc gtgtcagaca acgggttgtg
     9901 gatgtggtga aggatgagcg gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc
     9961 cctgtggtgg atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt
    10021 ctcccgtctc tgcaggtgag cgtaccgaaa ggtgttccga
//
