LOCUS       NZ_CP126880             2894 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_21 plasmid pEh21_2, complete
            sequence.
ACCESSION   NZ_CP126880
VERSION     NZ_CP126880.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394410
            Assembly: GCF_030848005.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848005.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:59:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,707
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,487
            CDSs (with protein)               :: 4,487
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 34 of 106
            Pseudo Genes (incomplete)         :: 77 of 106
            Pseudo Genes (internal stop)      :: 15 of 106
            Pseudo Genes (multiple problems)  :: 19 of 106
            Pseudo Genes (short protein)      :: 1 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126880.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_21"
                     /isolation_source="stools"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh21_2"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020-06-19"
     gene            complement(join(2790..2894,1..96))
                     /locus_tag="QPR63_RS23505"
                     /old_locus_tag="QPR63_23480"
     CDS             complement(join(2790..2894,1..96))
                     /locus_tag="QPR63_RS23505"
                     /old_locus_tag="QPR63_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_059363303.1"
                     /translation="MADDRTLQLRLTGFRKAEASLRLEGMDPSGTPLYESVKARLLSGE
                     ITYDEGRAEILAHHQKRANSN"
                     /name="antitoxin VbhA family protein"
     gene            complement(978..1262)
                     /locus_tag="QPR63_RS23510"
                     /old_locus_tag="QPR63_23485"
     CDS             complement(978..1262)
                     /locus_tag="QPR63_RS23510"
                     /old_locus_tag="QPR63_23485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_059363302.1"
                     /translation="MDTKFHFRLDENVKMRAQKAAEARGKTLSEACRDFAEQLAREVEP
                     LSQHEKWLKKQVELAVAKANSGDGRFTSNEEVKRVMASRKDEIRAKYKR"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            1452..1856
                     /locus_tag="QPR63_RS23515"
                     /old_locus_tag="QPR63_23490"
     CDS             1452..1856
                     /locus_tag="QPR63_RS23515"
                     /old_locus_tag="QPR63_23490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059363301.1"
                     /translation="MGFISQLSKTKISTRRGSASQITVTQSFKPSTSAEELSIRISTEC
                     LNNISLKIGDKVDVLYDADSDTWMIKKEAEGYKISGKPDAPTGLIRYTLKEGHKKFTST
                     RESLPVKKDSNDDTIVFMGDKMTFKLKESE"
                     /name="hypothetical protein"
     gene            1853..2149
                     /locus_tag="QPR63_RS23520"
                     /old_locus_tag="QPR63_23495"
     CDS             1853..2149
                     /locus_tag="QPR63_RS23520"
                     /old_locus_tag="QPR63_23495"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_077951050.1"
                     /translation="MMASNLIWEEEAIDDRADYFEWLYQQNPKAAEDADQYIEDTVDLL
                     IENPFLGKDSEIVSGVRVLIIKGLSLNVYYQEGHGTVKVLRVLHQSRNIAKLI"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          140..789
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.464"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcgttccg gaaggatcca tgccttcaag cctgagagat gcttcagcct tgcggaaccc
       61 ggtcaggcgc agttgcagtg tccgatcatc agccatgggt tttcctttga gcagtcatgc
      121 gaataaggca ggcgtaaagt gggcccactt tacatatcct gcccggatgt tgccgttgta
      181 cagctgtgca gggaaaagtg gaaaacggca ggaaagcgtt gcaccagagt gtgtaatcag
      241 agaggaaagt ggtgtaccga gcgatacgag ccctgttcag cattacagac attacgcttc
      301 ctcggtcatt cactcacttc gttcggtcat gacgctgcgg cgagcgttac cagctcaggc
      361 agaaagccat gtaaaaggtc tgtaggattg ccggttttcc ataggcttcg ccctcctgac
      421 gaacatcaca gaaatctggc gatttccggt gctcaagtca gaaggggcga aacccgacag
      481 gacttaaaga tccccaccgt ttccgatggg tcgctccctc gtgcgctctc ctgttcccac
      541 cctgaggttt acccgttgca gaccgccgta aggtggcgtt ttctcattcc tacccgtgca
      601 actgggttcc ttttaggtgg ttcgctccaa gctgggctgt atgcacgaac tccccgttca
      661 acccgactac cacgactttc aggtaactgt actcttgaat ccaaccctga aaggcacgac
      721 gaatcgccac tggcggtagc cattggtgac aggtttacgg agcggagcag tgtatataag
      781 tacacataga gttcttgaag agttagccta actacagcta cactggaagg acagatttgg
      841 tcgctgcgct ccgtttaagc cagtcaccac ggttcagaag ttccccaact tactgaacct
      901 tgaaaaaacc gcctccccag gcggtttttt cttttcaacg agattacgca tattcagtat
      961 tagcgtcaat atacaggcta gcgcttgtac ttagctctta tctcgtcttt tcttgaagcc
     1021 ataactcgct tcacttcctc attggaagtg aatctaccgt ctccagaatt agcttttgca
     1081 acagccagct ctacctgctt cttcagccat ttttcatgct gactaagagg ctcaacctct
     1141 cttgccaatt gctcagcaaa atctctgcaa gcttcactta gagttttgcc gcgggcttct
     1201 gccgctttct gggctctcat cttgacgttc tcatcaagcc tgaaatgaaa tttagtatcc
     1261 ataagatcct ccgacacccg taatgttagt acagttgtgc gcacaagcaa cacacatatt
     1321 tatataataa aaacccacta aaaacattga cttatataca atgatcatgt cggatagaaa
     1381 aaaacgaaga aggatgatga tcgatcagtg aagatcagtt aaaatacaaa caagagaaaa
     1441 aaggaggatg tatgggattc ataagccagc tttcaaaaac aaaaatatca acacgccggg
     1501 gatctgctag tcagataaca gtgacccaat cattcaagcc ttcaacgtca gcagaagagc
     1561 tttccatcag gatctcgact gaatgcctca acaacataag cttgaaaatc ggagataagg
     1621 ttgacgtact ttatgatgca gatagcgata catggatgat aaaaaaagag gcggaaggct
     1681 ataaaatatc cggaaaaccg gatgctccga caggactgat ccgctacaca ctgaaagaag
     1741 gccacaagaa atttaccagt acccgggaat cacttccggt taaaaaagat agtaatgacg
     1801 acaccatcgt attcatgggt gacaagatga cgtttaagct gaaggaaagc gaatgatggc
     1861 aagcaatctc atttgggaag aagaagctat tgatgatcgg gctgactact tcgaatggct
     1921 ttatcagcag aatccaaaag ctgctgaaga tgcagatcag tacatagaag acacagttga
     1981 cttactaata gagaatccat ttctcggcaa agatagtgaa atagtgtctg gcgtgagagt
     2041 tctcatcatc aagggcctat cgttaaatgt gtactatcaa gaaggccatg gaacagtaaa
     2101 agttctacga gttctccatc aatcacgaaa catagcaaaa ctcatttaag gcaacgcaac
     2161 ctgcctcagc gagggagaaa gaagcaactc aaaaaatgtt ttaacaatgt attttgtttt
     2221 ctagacagaa gacagagcca gtctacgccc ttataaatta ggcaaacagc tcaaatccta
     2281 aggaaaaagc tcattcaagt agagaccttt ttccttagga tatacgtatt taccataatc
     2341 ccttcttacg cgcaccctct gaaaggccct cagcgtcact ctgcaacgag ttagccattt
     2401 ccagcacctt tcctggccat aaccctgaaa aacgcacctg atgatttctg cgcgtctgca
     2461 tggctatgca cggaagtgaa tggaggatag tttcgcacgg atgttgtaac cagatgctca
     2521 tttccagcac agaactctct agataacggt gagcgggtgc gcagtttact gtgcgaccgt
     2581 gaggcgttgg gcaagccgcc aggcgcggca ggaatatcgc caaaggggtc ggatttaacg
     2641 aggagtggga ccgtagttcc tggcgggtgt cgggcggagc ctgaccagga ggatttggcg
     2701 tgccggcggt actccaaaaa cacatcgtgt cccgctgttt tgtggcgccg gcgcagctgg
     2761 ctgtggtcac aaaacatttc ctgtccgcgt cagttactgt ttgcccgctt ctggtgatgg
     2821 gccagtatct cagcgcggcc ttcgtcgtag gtgatctcac cggaaagaag ccgcgctttt
     2881 acggactcat agag
//
