LOCUS       NZ_CP126868             9923 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain 21KM1498 plasmid pKM98_p4, complete
            sequence.
ACCESSION   NZ_CP126868
VERSION     NZ_CP126868.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394388
            Assembly: GCF_030847805.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030847805.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:06:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,886
            CDSs (total)                      :: 4,768
            Genes (coding)                    :: 4,668
            CDSs (with protein)               :: 4,668
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 58 of 100
            Pseudo Genes (incomplete)         :: 47 of 100
            Pseudo Genes (internal stop)      :: 9 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126868.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="21KM1498"
                     /isolation_source="central vein catheter"
                     /host="Dog"
                     /db_xref="taxon:158836"
                     /plasmid="pKM98_p4"
                     /geo_loc_name="Switzerland"
                     /collection_date="2021-06-22"
     gene            386..544
                     /locus_tag="QPR64_RS24375"
     CDS             386..544
                     /locus_tag="QPR64_RS24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(781..1149)
                     /locus_tag="QPR64_RS24380"
                     /old_locus_tag="QPR64_24345"
     CDS             complement(781..1149)
                     /locus_tag="QPR64_RS24380"
                     /old_locus_tag="QPR64_24345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704866.1"
                     /translation="MALLISLVVVAGLYYQFWYKPHRAPILRIWGIAPDGQEFRLLLTE
                     HGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYLEDAHKNELLIELNESDLYPGGAIELQ
                     HNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            1309..1542
                     /locus_tag="QPR64_RS24385"
                     /old_locus_tag="QPR64_24350"
     CDS             1309..1542
                     /locus_tag="QPR64_RS24385"
                     /old_locus_tag="QPR64_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_044704865.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDGRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1564..1791
                     /locus_tag="QPR64_RS24390"
                     /old_locus_tag="QPR64_24355"
     CDS             1564..1791
                     /locus_tag="QPR64_RS24390"
                     /old_locus_tag="QPR64_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2393..5278)
                     /locus_tag="QPR64_RS24395"
                     /old_locus_tag="QPR64_24360"
     CDS             complement(2393..5278)
                     /locus_tag="QPR64_RS24395"
                     /old_locus_tag="QPR64_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_001138082.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRRA
                     HNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHEHL
                     AELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATSTIE
                     RLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGSNSSS
                     ANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLAILAVC
                     VSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIGGALVDA
                     QDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRYAPRMLRL
                     LDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDARLWEIAVLF
                     HLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLADRQARLDMRL
                     RELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPTRITDLLLEVD
                     AATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHTFWELIRIGRWH
                     VEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEAMNLVNAKYGNTP
                     GLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQFTDTGGFTDHVFA
                     ACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAMIERNWPDILRIAAT
                     IAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWILDAELQRRAQIGLNK
                     GEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIFWNTMKLGEVVANQKRD
                     GKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
                     /name="Tn3 family transposase"
     gene            5404..6018
                     /locus_tag="QPR64_RS24400"
                     /old_locus_tag="QPR64_24365"
     CDS             5404..6018
                     /locus_tag="QPR64_RS24400"
                     /old_locus_tag="QPR64_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000904906.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDD
                     RPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQIDTT
                     TASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQAAMA
                     QRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT"
                     /name="recombinase family protein"
     gene            6084..6887
                     /gene="aph(3'')-Ib"
                     /locus_tag="QPR64_RS24405"
                     /old_locus_tag="QPR64_24370"
     ARG             6085..6884
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6887..7723
                     /locus_tag="QPR64_RS24410"
                     /old_locus_tag="QPR64_24375"
     CDS             6887..7723
                     /locus_tag="QPR64_RS24410"
                     /old_locus_tag="QPR64_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(7958..8173)
                     /locus_tag="QPR64_RS24415"
                     /old_locus_tag="QPR64_24380"
     CDS             complement(7958..8173)
                     /locus_tag="QPR64_RS24415"
                     /old_locus_tag="QPR64_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_044704874.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8180..8671)
                     /locus_tag="QPR64_RS24420"
                     /old_locus_tag="QPR64_24385"
     CDS             complement(8180..8671)
                     /locus_tag="QPR64_RS24420"
                     /old_locus_tag="QPR64_24385"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_063844470.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRAWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<8706..9410)
                     /locus_tag="QPR64_RS24425"
                     /pseudo=""
     CDS             complement(<8706..9410)
                     /locus_tag="QPR64_RS24425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9349..9672)
                     /locus_tag="QPR64_RS24430"
                     /old_locus_tag="QPR64_24395"
     CDS             complement(9349..9672)
                     /locus_tag="QPR64_RS24430"
                     /old_locus_tag="QPR64_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170949721.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLEQLRHAVLEKGTD
                     DDH"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             6888..7720
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9779..9857)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.75"
                     /coverage="100.0"
                     /evalue="2.7400000000000003e-32"
     Typing          10..498
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.345"
                     /coverage="94.0"
                     /evalue="1.69e-147"
     Typing          complement(7867..9359)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.8"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9356..9672)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.92e-61"
ORIGIN
        1 atgcctgtcc cggtggatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
       61 ggctgcggcg agcggtacgg actcacggat aaagacgtgg gggggaaata cgtgaatgca
      121 ggaggggaaa aaaggatgcg gcgctgccgg ttttccatag gctccgcccc cctgacaagc
      181 atcacagaat ctgacgctca aatcagtggt ggcgaaaccc gatgcggctt aaagatacca
      241 ggcgtttccc cctgatggct ccctcgtgct cccccgcatt cctgcctttc ggtttaccgg
      301 tgtcattccg ctgttatggc cgcggtttct cattccacgc ctgacactca gttccgcgaa
      361 ggcagttcgc tccaggattg actgtatgca cgaacccccc gttcagtccg actgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggaaaga cacgaaaaat cgctactggc
      481 agcagccatt agtaactgga ggtatacgag agacaaatta agaggtcttg aagtatgggc
      541 ctgagagagg ctacactgaa agaacatttt tggtttctgc ggtctcgtat acccagttac
      601 ctggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc caggtggttt
      661 tttcgttttc agagcaagag attacgactg taacgacagg atcacaagaa gatcatttac
      721 tcagagagac taactaccac tatgtttgcc tatcaacata cttaggaaac attacttgag
      781 ttaacgactc caccttactt ttagtatctc gcaatgaatc ttccgaccgc cgttgtgttg
      841 aagttctatt gcgccaccgg ggtataaatc actttcgttt aactcaatta aaagctcgtt
      901 cttgtgagca tcctctaaat aaaggtaata acacccacca taaaggttct gcgtatctgg
      961 cacggcaaca aaagatttct ttccgaacag taaaacagcc tctttttctc catgctctgt
     1021 tagcaataat cgaaattctt gtccatcagg cgcgatcccc catatacgca agatcggcgc
     1081 tctatgtggc ttataccaaa attgatagta aagacctgcc actacaacca gtgaaattaa
     1141 taaggccatt tcaatcctca aaaacaagca acattccaaa atcgtttttc attcattttt
     1201 actgctgggc atgatgccca ccttcaatcc tcttttcgca tgaaaaaacg acctatactt
     1261 agatggttag tgaccatatc cactttacct ttaagcaggg aacctattat gaataaaaaa
     1321 acgctgttgg cggccatgat ggtcgcgttg gtgacctttg ccgctgctgg atgttcttca
     1381 aaccaagaaa taaaaaccac agatggtcgc actatcgtta cagacggcaa acccaaagtt
     1441 gacagtgata cggggttagt atcctacaaa gatgcccata caggccgaac cgaacagatc
     1501 aaccgtgaca aaattaccaa catgagtgaa ctagacaact gatacggata aaaggagctg
     1561 gctttgaaaa agatttttgt ttcagtgttc gcagcggcag ttgcgctttc agccctaacc
     1621 ggctgcacgc gcacaagcta tgccatccac accaacgacg ggcgtacaat tgtcagcgac
     1681 ggcaagccta cagaatcaga ctcaggcctg ctgggctata aagacgcaaa tggtgtcaaa
     1741 cagcagatca ataaagccga cgttaaagaa gtctctgaaa ttccgcattg aggcatcgct
     1801 ctctccgaaa agggccgcta atgcggccct tttctttatc tgccccaaca acaggcaaaa
     1861 aaagcccccg ctgggggcca aatacctgca gtggctggat agtactgcat tcctgatgaa
     1921 ggagacgaat aagtatgggc cggatttttg cgtctgcagg ctcggtcgcc acaatttttc
     1981 cgtacccgcc aggcatcatg cttttaccat aacatccgtt gtctgcaccg ccggatcggc
     2041 actgacggcg cagacggcac ctggcacccg aaaacccggc ttaagcaacg ctacatcctc
     2101 cgttaagccg ggagataatt tttacccgac tgcatagcca tgcagccagg tgaatgacgg
     2161 caggattcag cccaaaaaca gcgtgaaggc cccgaaaacc ggcaaatgct tttccagcca
     2221 ctcccacagt tctgccgcca gatgcaggca gttaagtaca gcttcaataa cattaagcag
     2281 tttcataatt ccccctggta taggccgtga tctttgcatc acgacgtatt tagcggggaa
     2341 tttcgcagcc cttcttcttg gggtcgtttg cgggaggggg cggaatccta cgctaaggct
     2401 ttggccagcg atattctccg gtgagattga tgtgttccca tccgagcggc gaaacatggg
     2461 ccaagagatc gggcgatagc agctttccat cgcgtttctg gtttgcaacg acctcgccga
     2521 gcttcatggt gttccagaag atgatgatgg cggcgagcag attcatgccg gcgatgcggt
     2581 aatgctggcc ttcggcggaa cggtcgcgga tttcaccgcg gcggtggaag ctgattgccc
     2641 gcttcagcgc atgatgagct tcgcctttgt tgagcccgat ctgggcacgc cgttggagtt
     2701 cggcatccag aatccagtcg atcatgaaca gggtgcgctc gacgcgaccg acttcccgca
     2761 gggctgtcgc gagctcgttc tgccgcggat aggaggcgag tttccgcaga atctggcttg
     2821 gcgcgacggt cccggcagca atggtggcgg cgatgcgcag gatgtcgggc caattgcgct
     2881 cgatcatggc ttggttgacc tttccgccga tcaacgctcg caggtgcgcc ggggcggccg
     2941 acggattgaa cgcgtagagc cgtttggatg gcaggtcgcg gatgcgcgga gcgaaccggt
     3001 agccgagaat ggcacatgcg gcaaagacgt gatcggtgaa gccgcccgtg tcggtgaact
     3061 gctcgcggat atggcgtcca gcatcgttca tcagcaggcc atcgaggatg taaggcgctt
     3121 cgcttgccgt tgcaggaatc acctgggttg cgaacggcgc atattggtcg gagacgtggc
     3181 tataggcttt caggcccggg gtattgccat atttcgcgtt gaccaggttc atggcctcac
     3241 cttgctctgt agcgacgaag aactgtccgt cgctcgaagc cgacgtgccc atgccccaga
     3301 accgggccat gggtaacgct gcctgtgcct cgaccaccat ggccagcgcc cggtcatagg
     3361 cttcgccctc gacatgccac cgtccaatgc ggatcaattc ccagaaggtg tgggtgtttg
     3421 tcgcatccgc cattttgcgc aagccgaggt tgatcccttc cgccaagata acgttcatta
     3481 gcccgatccg gtcagcgcag ggtgctcctg tgcgcagatg ggtgaacgct tcggtgaagc
     3541 cggtcgccgc atccacctcc agcaggagat cggtgatgcg cgtgggcggg atctgcttgt
     3601 agagatcgag caccagatct tcggcgcctg tcggcgcggc ggcttcgagt ttctcgatat
     3661 gcagaacgcc gttttcaatc gacccgcccg ggatcgtgcc tgcgcgagcg gcacggccaa
     3721 gctcgcgcaa ccgcatgtcg aggcgagctt gccggtctgc cagccattcc tccggccgca
     3781 atggcacagc gagacgaccg ccttccgcga tggattgtgc cggaacgagt gcgtgtttca
     3841 gatcgccata gcgccgggac ctagtaagcc agacatctcc ggagcggaac gcatcgcgca
     3901 gatggaacag caccgcgatc tcccataggc gagcgtcgcc agccctctgg gcccgaaggt
     3961 ggcgatgcca tttcgagctg ggccgcaaga agctggtcat cgcggcatcg ttcaaaccgg
     4021 tacgaagggc cgtcaccgct tccagaagcg gcagtgcaac gggcgcagct cgcagatcga
     4081 gcaggcgcaa catgcgtgga gcgtatcggc ggaagcggtg ataaccgtcg agcacatgat
     4141 tgagcggatc gtcggccatg gtggcggtca gcctggttgc cattgcaaca agggttttta
     4201 agccgtccca ccctgaccca ctcgcgatga catcgcccag cggctggcca tcatcctgtg
     4261 catcgaccag ggcgcccccg atctcggcga aggatttcag ggtgtcacgc accacccccg
     4321 cttcgtctgc gacctttgca tggcaaatac gctccgaagc acggtagaga cggccgacga
     4381 tccggtcgtg ggtttcgacc actgcgtcgg ccaacatcgc ctgccattcc gagacgcaaa
     4441 cagccaagat cgcaagccgc ctgtcctccg ggagatcgcg catgccgtcg gcataatacc
     4501 gttcaccctg cctgcgcaga cgagtcaccc gatgggcagg aacgccggca agcagatcct
     4561 cggggagatc gatgcgttgc agatattcga gccggtcgag cagccggttg gccgacgaag
     4621 agttcgagcc aggctcgaac tggcgcagcc acacaaaacg ggtcacccga tcatcagccg
     4681 tctcctcgag caatgccagc aactgttctc ggatcgacat aggcagccga ctggcgatcc
     4741 tcgtctcgat gcgtcgctcg gcatcgacga gagccgcggc acaaagccgc tcgatcgtgg
     4801 atgtcgcggg aaggacagtg cgggtgcgtc ggcactcggc tacgaagcga cgggcgatat
     4861 cctcgttcga caccgccatc tcggcttctc ggaacaacca ttccttcagc tcgctcgcac
     4921 cacgtccgga gaaggtgcgg aagccgtaga gcccccgtaa ctcggcaaga tgctcgtgcc
     4981 gtgtttcctc gcgggcagca tagtctacga gatcgtcggc acccaggcca agctgcgctc
     5041 cgataaattc gatgacctct gcagggatca gttcgcctgg agccagcacc cggccgggat
     5101 agcgcaggac acacaattgc agggcgaagc cgaacctgtt gtgagcgcgc cgacgcagcc
     5161 tgatatgccc aaggtcttca tcactcagcg tatagtgctt gagcaaatcc gtctgtgaag
     5221 tcggcaagcg caacagcgcg tctttctgcc gatcggttag agtgacgcga cgcggcatac
     5281 atgttccttt ttcaaaatct gatagcgttc aagacgcttt gtttatgaag ctggttgaga
     5341 tacatttcca gaggtcaatg caatcgtggc cgaagcgccg cctcaaacca acgtttgtga
     5401 tacatgctga tcggatatgc ccgcgtctcc aaagccgatg gctcgcagtc tctcgacctg
     5461 cagcacgacg ccttgcgcgc cgcaggtgtc gaacgggaca atatctatga tgatcttgct
     5521 tccggcggtc gtgatgatcg ccctggcttg actgcctgcc tcaagtcatt gcgtgacggc
     5581 gatgtgctgg tggtctggaa gctcgatcgc ctcggacgat cgcttgccca tctggtcaac
     5641 acggtgaagg agctgtcaga ccgcaagatc ggcctgcggg ttctgactgg aaagggcgct
     5701 cagatcgaca ccacgactgc gtccggtcgc atggtgttcg gaatcttcgc caccttggcc
     5761 gagttcgagc gggatctgat ccgagagcgc accatggcgg gtctcgcctc cgcgagagcg
     5821 cgcggtcgca agggcggacg aaaattcgcg ctcaccaaag ctcaggtgcg tctcgcgcaa
     5881 gccgccatgg cccagcgcga tacttcagtt tccgatctct gcaaggaact cggcatcgag
     5941 cgcgtcactc tctaccgata tgtcggtccc aaaggcgagc tcagagacca tggaaagcat
     6001 gttctcggac ttacgtagca actcgtttct tttcgcaggt tgagccacct ccgcgcttca
     6061 tcagaaaact gaaggaacct ccattgaatc gaactaatat tttttttggt gaatcgcatt
     6121 ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttttcga cgtggtgacg
     6181 ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg
     6241 accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtgatc aactggcagg
     6301 aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg gcggctgatc
     6361 tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc
     6421 acagcctatc ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg
     6481 ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac gaggacaaga
     6541 gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc
     6601 aagagcgcac cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg
     6661 accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc
     6721 gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg
     6781 aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg
     6841 aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc
     6901 tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag
     6961 cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc
     7021 tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag
     7081 gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga
     7141 gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc
     7201 ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc
     7261 gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg
     7321 tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga
     7381 actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg
     7441 gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt
     7501 ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc
     7561 ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc
     7621 ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc
     7681 tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc
     7741 ctatcccctg ggaacacatc aatcttaccg gagaatatcg ttggccaaag ccttagcgta
     7801 ggatttcgcc ctctcccgca aacgacccct tcttggggtt tatcacgggc cggcatccgt
     7861 cagcccagcg tcggtccttt gtaggttttc tgcggctggc gctccagctc cagtgttttc
     7921 tgatactcat tctccccctg ccggaactcg gtggcattca gttcccggac aagcggcgca
     7981 gctgctcgct taaactcagc acctgcgtgc tcaagtccga cacgcgctcg ttcagcaccg
     8041 cgttctcccg ttgcataagc ccagacatct tctgccattc cgcgaaggcg ctctcccatt
     8101 cgtccagcct ttgcgagtag tcctgatgta gctgctctaa tgcggtcagc aactgctttt
     8161 ccagctctgt cattttccgc tactcctgtt tcaggcggat ccaggtcgtc ccgtcgtagg
     8221 ggatggcttc ggcttgcatc cccggcggca ggataaggaa cctccggccg tcgctgctct
     8281 cctgatagcg cactccccac gtccgggcag tcattttcgc cagggtatct tcctgctgac
     8341 gaagatgtgt gtaattgtcg gttatctgct gcccctgcca ccacagaatg ctcccgctcg
     8401 tggcgatcag cagcgctgac accatcagga tggtgagcca tgccctcccg gctgtcagca
     8461 gcacgctccg ccggtttttc tccatcgcct cgctcatact ctgttcgtgg gcgctgatgg
     8521 cgtcgctgat tctcctcgcg cttgcgctca gttccgctct gacagactgc tcgtgctcgc
     8581 tgaatgcggc tttcagcatc tcgccggtac tctgctgctg cgctttcgat ttctgctcca
     8641 agtccttcgc cagcgttaaa agactgctca tagatggctc ccttcagtcg gatgtttcgc
     8701 cccccgtccg ggtcggcaat gctgatgctg cttttcgtgc tgcgcaccac ctcaaaacct
     8761 gcgctttcca gcgcctcagt gacgtcctga cgggttttca gctccccgga tgaggcaagg
     8821 gcaagcaggc cgcgcgtaat cgcctgggca gcttcctgct tcgcttccgg cagcgcggac
     8881 ggcgtgacca gcgcccgccg gttttccggc gcgttcgggt cgtgcagccc cagccggccg
     8941 ttgaccaccg tctgccaggc atcgatgcgc ggccggtcgg cgcggtcgta gtacggctgg
     9001 agccgcctgc cggtcagcag ctcggtattg gggatcagaa agttcagctc cagacgcccc
     9061 ttgtccgcgt gctccaccca cagaatgctg tactgatctt tatcgaggcc gggcatcaga
     9121 acacgctcga agctggccat cagtttttcc cgctggccgg gaggcagatc tgcctccgca
     9181 aacgacagta cgccggaggt gtatttcttc acgtaaggcg acacatcaat cagctcccgg
     9241 acttcttccg gcttgccctg caggacggtg gcgccttcac gctgccggtc tttacccagc
     9301 aaatagtcca ccggcccggc gccaccgccg cgaccgcgcg gatgaaattt aatgatcatc
     9361 gtccgttcct ttctcaagaa cggcgtgccg cagctgctca aggccggcat cgattgccat
     9421 cagcgccgcc acaatctgca ccctgtcatg gccggtaccg ccgccggcgt taacgcggcg
     9481 ggctatctgg ttcaggttgt tgcccatgcc ggcaagctgg cgcagcagcg ccggcgagat
     9541 cgagggtagc tttccggtac gcgcaggctt ttcatcaaga caggtctgcc gcatccacgc
     9601 cgccagctgc ctgccgtcac agcgttcgag cagccgccgg tgctcgtcct ccgtcaccca
     9661 catggtgagc atcttgttcc gtttctctga caaaccggtc tcctgataac gccgggacgg
     9721 gcgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcaggctttt
     9781 ggcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc
     9841 ttactatggt ggcacgacgg gaatcgggat gaaatagtca gtcataaaaa ctgtgagatg
     9901 aggctcaggt gcgtaagggg ctg
//
