LOCUS       NZ_CP126858             4665 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_7 plasmid pEh7_7, complete
            sequence.
ACCESSION   NZ_CP126858
VERSION     NZ_CP126858.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394390
            Assembly: GCF_032202685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_032202685.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:01:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,900
            CDSs (with protein)               :: 4,900
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 25 of 93
            Pseudo Genes (incomplete)         :: 69 of 93
            Pseudo Genes (internal stop)      :: 13 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126858.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_7"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh7_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-12-07"
     gene            760..1083
                     /locus_tag="QPR83_RS25485"
                     /old_locus_tag="QPR83_25465"
     CDS             760..1083
                     /locus_tag="QPR83_RS25485"
                     /old_locus_tag="QPR83_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1292..>1726
                     /locus_tag="QPR83_RS25490"
                     /old_locus_tag="QPR83_25470"
                     /pseudo=""
     CDS             1292..>1726
                     /locus_tag="QPR83_RS25490"
                     /old_locus_tag="QPR83_25470"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1761..2246
                     /locus_tag="QPR83_RS25495"
                     /old_locus_tag="QPR83_25475"
     CDS             1761..2246
                     /locus_tag="QPR83_RS25495"
                     /old_locus_tag="QPR83_25475"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2250..2465
                     /locus_tag="QPR83_RS25500"
                     /old_locus_tag="QPR83_25480"
     CDS             2250..2465
                     /locus_tag="QPR83_RS25500"
                     /old_locus_tag="QPR83_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2811..4367
                     /locus_tag="QPR83_RS25505"
                     /old_locus_tag="QPR83_25485"
     CDS             2811..4367
                     /locus_tag="QPR83_RS25505"
                     /old_locus_tag="QPR83_25485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          474..647
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(3..550)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.848"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          761..1077
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
     Typing          1074..2569
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttactaat ggctaccgcc actggcgatt tttcgtgtct gttccgggtt ggactcaaga
       61 tgatagttac cgggacaacg tggtagtcgg gctgaacggg gggttcgtgc atacagtcca
      121 gcttggagcg aactgccttc ccggaaccga gtgtcaggcg tggaatgaga taaacgcggc
      181 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
      241 ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac cactgatttg
      301 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg
      361 cggccttctc gcttccctgt tacgttcctt cctggcatct tccaggaaat ttccgcccca
      421 cctgtaagcc ggcaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      481 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc gccggtgccg ccttttttct
      541 cctgccacat gaagcacttc actgacaccc tcatccgtgc caacatatta agccagtata
      601 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc caaaagcctg
      661 acgcgcctgc ggcttgtcca accccgtgcc gaccgggaaa ggttttcccg cccgtcccgg
      721 cgttatcagg agaccggttt gtcagagaaa cggaacaaga tgctcaccat gtgggtgacg
      781 gaggacgagc accggcggct gcttgagcgc tgtgacggca ggcagctggc ggcgtggatg
      841 cggcagacct gcctggacga aaagccggca cgttccggaa agctcccctc aatctcgccg
      901 gcgctgctgc gccagcttgc cggcatgggc aataacctga accagatagc ccgccgtgtt
      961 aacgccggcg gcggtaccgg ccatgacagg gtgcagattg ttgcggcgct gatggccatc
     1021 gatgccggac ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt
     1081 taaatttcat ccacgcggtc gcggcggtgg cgccgggccg gtggactatc tgctgggtaa
     1141 agaccggcag cgtgaaggcg ccaccgtcct gcaggggaag ccggaagaag tccgggagct
     1201 gattgacgcg tcgccttacg ttaagaaata cacctccggg gtactgtcgt ttgcggaagc
     1261 ggatttacct tccggccagc gggaaaagct gatggcaagc tttgagcggg ttctgatgcc
     1321 cggacttgat aaagaccagt acagcatcct gtgggtggag catcgtgaca aggggcggct
     1381 tgagctgaat tttctgatcc cgaacacgga gctgctgacc ggcagacggc tccagccgta
     1441 ttacgatcgc gccgaccgtc cacgcatcga tgcctggcag accatcgtga acggcagact
     1501 ggggctgcac gacccgaacg caccggagaa ccggcgggca ctggtcacac cgtctgcact
     1561 gcctaaagcg aagcaggagg ctgcagaagc gattacacgc ggtctgcttg ctcttgcctc
     1621 atccggggag ctgaaaaccc gtcaggatgt cactgaggcg ctggaaagcg caggttttga
     1681 ggtggtccgc accacaaaaa gcagtatcag cattgccgac ccggacgggg ggcgaaacat
     1741 ccgactgaag ggagccattt atgaacagtc ttttaacgct ggcgaaggac ttagagcaga
     1801 aatcgaaagc gcagcagcag aataccggcg agatgctgaa agccgcattc agcgagcacg
     1861 agaaatctgt aaaagcggaa ctgaacgaaa gcgcgaagag aatcagcgcc gccatcctcg
     1921 accacgaccg gaaactgtcc tcagccatga gccagcgcac gaaaggcatg gtgcgcatgg
     1981 tcagccagac gtggctgacc atcgtcctgg tctccgcgct gctgatcgcc tcgagcgcgg
     2041 gcattctgtg gtggcagggg cagcagatac tcgacaacta cacgaccatc cgggagcaga
     2101 agagcacgca ggccatgctg tcagagagga acagcggcgt gcagctctcg acctgcggcg
     2161 agcagagacg ccgctgcgtg aaggtgaacc cggaagcggg acggttcgga gaggattcga
     2221 gctggatgat actggcgggg aaatagcaca tgacggagct ggaaaaacag ttgctgagcg
     2281 cattagagca gctacagcag gactactcga aaaggctgga cgagtgggag agcgccttcg
     2341 cggaatggcg gacgatgtct ggtcttatgc tacgggagaa cgcagcgctg agcgagcgcg
     2401 tcacgggctt gagcaggcag gtgcagagtt tgagcgagca gctgcgccgg ttgtcgagcg
     2461 actgaatgcc gtcgagctgc accgggagca ggagcgcgtg gtgcagcatc agaaagcgct
     2521 ggagctggag cgatctcagc gacagcggac gtataacagc ccttcactgt gataacctga
     2581 taaaacgggg ctttcggcgg acatctgcgc tgaaagccgg agccattcac ccgactgcat
     2641 agccatgcag ccgggtgaaa tttatctggc gcagaatgcg cggtttagcg gagagtttgc
     2701 tgccggtttt accgggctga cgccgcattt gttccgaacc cgtccggggc ggtgctgata
     2761 agggatgtta tggtaaatgc ggcatttttt attatatgga gtgtattttt atgagaatta
     2821 aaaagcctca ccttcgtccg atagtcataa ccttgtccgt tggtgggatt ttgttaactt
     2881 ctatttttct gattgccgtt attatggttt ttcagcgcga aaatatagaa aaagatttgc
     2941 tttatgctaa ttcatcgtac gctatgaaaa tgtcagatgt aatgagtggt tatattgaaa
     3001 tggctcaagg tgaattagca tatggagcaa aaaaaataaa atcaactaat gatattgact
     3061 cacttaaaga tgaggcggat aggttaagat tgcagtctgg tatgtttaat tcagttcttg
     3121 ttgttagtaa tgatgctgtt gttctggcga cttcgccaga atctcttgat ttagttggta
     3181 cccgcttgaa ttcaagtgcc agcaaattgg cgattgagga aaagaaacca ttcatatctt
     3241 caccgttaaa atcagttact gggaatttga ttattttgtt gtcacatcct atttatgata
     3301 agggtggcaa ttacataggg tatgtcggtg ggtctatata tctaaaaaaa cataatttat
     3361 ttggcgatgt tttgagcagg cattttttca agaacaacac tgaaattagc attgttagtg
     3421 atgatgggaa tgtgatcttt aataacgata aaagtgctgt tgatcggcct atggttatgt
     3481 ctgatgattt gaaatcaaag ttaactgcgt ctgatagagg tgagggggca tttttttctt
     3541 cggggcatag atatcttctt ggttatgcta gtatgaaaaa tactgcttgg aatgtgttgg
     3601 tttatagtcg tgctgacagt gtgactacta ttcttattgc ttatgctaaa agtgtacttg
     3661 ttctgctaat tataataatt attgtgtttt cacttatttc atatttcatt gcctccagta
     3721 tttcacttcc tttagaaaag cttgctatat ctaccaatgc taaagatatc ggagcttcgc
     3781 tagtttatat taaagctatc aatgcttggt atgccgaagc tgataggctt aaaaaggcgc
     3841 tttttacgta tattaaggta atgatggaaa gagttgattt tctaaatgag gtggcgctta
     3901 aagatcccct taccggcgtg aacaatagat tggggttttt aagtaaaata cagaatcata
     3961 agcgggattc agggtattca gttattgtaa ttgatgttga tttttttaaa gagataaatg
     4021 acagtttcgg gcatggggtc ggagatgagg tgcttgtttc tttggctcat attattaatt
     4081 cctgttgccg tagtgaagat gttgtttgtc ggtttggtgg cgaagagttt gttgttttcc
     4141 tgccgaatac atctgtggta acagctaagt gtgttgcgga gcgtattagg agtgttattg
     4201 aaagtactgt cttccctaat aacttgcggg tcaccatatc tgctggtgtg gcaacacaag
     4261 atgatcctct tggtaggatc gattttttgc tgaaaaatgc tgacgatgct ctttatcaag
     4321 ccaaaaggga ggggcgtaat aaagtgattg tatatagcgc tatttgagtt ttttgatgat
     4381 gctttacgcg ctagtgtgtc gttttttttg tggcgaagag tgggcaagaa gcggatcctc
     4441 ttgttatccc ttcgatgtcg tcgtaatctc ataccctgta aacaaaaaaa ccaccgctac
     4501 caacggtggt tcgtttgccg gaattcagag ctaccaactc tttcttccga aggtaactgg
     4561 ctttcacgga gcgcaggtac caaaactgtc cttccagtgt agccgcagtc aggccaccac
     4621 ttcaagaact ctgtagaaca ctctactact ccgctccgtg aatcc
//
