LOCUS       NZ_CP126856             5986 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_7 plasmid pEh7_5, complete
            sequence.
ACCESSION   NZ_CP126856
VERSION     NZ_CP126856.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394390
            Assembly: GCF_032202685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_032202685.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:01:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,993
            Genes (coding)                    :: 4,900
            CDSs (with protein)               :: 4,900
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 25 of 93
            Pseudo Genes (incomplete)         :: 69 of 93
            Pseudo Genes (internal stop)      :: 13 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126856.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_7"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh7_5"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-12-07"
     gene            complement(208..339)
                     /locus_tag="QPR83_RS25395"
                     /old_locus_tag="QPR83_25380"
     CDS             complement(208..339)
                     /locus_tag="QPR83_RS25395"
                     /old_locus_tag="QPR83_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074172424.1"
                     /translation="MHEPPVQSDCCACSGNYQLESNPERHDKAPLAAATGKQDVREM"
                     /name="replication initiation protein"
     gene            987..1310
                     /locus_tag="QPR83_RS25400"
                     /old_locus_tag="QPR83_25385"
     CDS             987..1310
                     /locus_tag="QPR83_RS25400"
                     /old_locus_tag="QPR83_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_029593203.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1519..>1959
                     /locus_tag="QPR83_RS25405"
                     /old_locus_tag="QPR83_25390"
                     /pseudo=""
     CDS             1519..>1959
                     /locus_tag="QPR83_RS25405"
                     /old_locus_tag="QPR83_25390"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1988..2485
                     /locus_tag="QPR83_RS25410"
                     /old_locus_tag="QPR83_25395"
     CDS             1988..2485
                     /locus_tag="QPR83_RS25410"
                     /old_locus_tag="QPR83_25395"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_164474471.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVGAELSASA
                     RRISDAILAHELSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGASGSILWWQGQQIT
                     DNYTTLRQQEDALAKMSAKTWGVSYQSDSNGRFLVLPKGAKAETGWTVDSGKRNAVKLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            2492..2707
                     /locus_tag="QPR83_RS25415"
                     /old_locus_tag="QPR83_25400"
     CDS             2492..2707
                     /locus_tag="QPR83_RS25415"
                     /old_locus_tag="QPR83_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_164474472.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFEEWRRMSGLMQRENA
                     MLNERVSGLSRQVQSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2888..3394
                     /locus_tag="QPR83_RS25420"
                     /old_locus_tag="QPR83_25405"
     CDS             2888..3394
                     /locus_tag="QPR83_RS25420"
                     /old_locus_tag="QPR83_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933225.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_164474473.1"
                     /translation="MVNESSRRLTNFTTVLLLLIKECRLERGVHQAQLAEKINKSPSSF
                     AKIETGKSPLTMEVFLACCSTLMISPSAVMSTAERYGNLLTNNGWILLNSSLEENDDEL
                     MKESQEYYSSPGYKSRLNMTSALNGPFYYPNGNVDGLAVFLFAISTHFKNQQLEAQNSL
                     LSGWN"
                     /name="helix-turn-helix transcriptional regulator"
     gene            4071..4595
                     /locus_tag="QPR83_RS25425"
                     /old_locus_tag="QPR83_25410"
     CDS             4071..4595
                     /locus_tag="QPR83_RS25425"
                     /old_locus_tag="QPR83_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063959429.1"
                     /translation="MQKVTDLYPPEIARHNLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVVHSLKQKYLPVTAITISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKQTNAQKEMSRLYKEFGEDGILALLRNVSRSNGN
                     GTPPPSGQPAS"
                     /name="hypothetical protein"
     gene            complement(4722..5150)
                     /locus_tag="QPR83_RS25430"
                     /old_locus_tag="QPR83_25415"
     CDS             complement(4722..5150)
                     /locus_tag="QPR83_RS25430"
                     /old_locus_tag="QPR83_25415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063959428.1"
                     /translation="MRVVIDIEESIVHDILNHANENEDGMSFEETVASLLNDAVNSKKT
                     KTLNDEEINQAILQMVSFAIKNRKENKSFKANELYFKALDDSWNRLSPSTRKSLGRRFR
                     TFVNELWDKATEGEFVIEFKNRNINNAAIYEVVKKVDL"
                     /name="hypothetical protein"
     gene            complement(5461..5976)
                     /locus_tag="QPR83_RS25435"
                     /old_locus_tag="QPR83_25420"
     CDS             complement(5461..5976)
                     /locus_tag="QPR83_RS25435"
                     /old_locus_tag="QPR83_25420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_133257823.1"
                     /translation="MAPPSSTLMNDPRVIGAVIAALLAFLAPTIRDFWITNRTKKNLVK
                     ILIVDVNARIKKIDKFLVPFQHAINKAKLEDTYMPWVSYNEGLEDHIDWKDEKWLMPKD
                     LVSEIVGFYSNTRSLIKFIETINSDRYKEISKDRQIAMLEGLLGDLKESYMEGFTLLQL
                     LQAKQGGR"
                     /name="hypothetical protein"
     Typing          700..873
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(193..719)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.084"
                     /coverage="87.0"
                     /evalue="0.0"
     Typing          1301..2802
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.392"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actccatgta agggaagagt aagaattgac gataagttat tataaagcgt aagctaacaa
       61 acgaaaaaac cacccggcaa ggtggttttt cgaaggtcca gcaaaccggc aagtttctga
      121 accttggtaa cagtcttgtg cgagacgtgt caccaaatct gtccttccag tgtagcctca
      181 ctcaggccac tacttcaaga actcttgcta catctctcgc acatcctgtt taccagtggc
      241 tgctgccagt ggcgctttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
      301 acaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
      361 tgccttcccg gaaccgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggagt
      421 gacaccggca accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac
      481 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagattctg
      541 tgatgcttgt caggggggcg gagcctatgg aaaaacggct tcgccgaggc cttgtcgttt
      601 ccctgttaag cttcttcctg gcatctacca ggaaatttcc gccccgttca taagcccgca
      661 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatatc
      721 tcctgtatcg catattctgc tgacgcgcta gtgccacctt ttttctcctt ccacatgaag
      781 cacttcactg acatcctcat ccgtgccaac atattaagcc agtatacact ccgctagcgc
      841 tgcgtgactg gttcagggct gcgccccgaa acccgctaaa agccactgac gcgcctgcgg
      901 cttgtccaac cccggccgac cgggaaaggt tttcccgccc gtcccggcgt tatcaggaga
      961 ccggtttgtc agagaaacgg aacaagatgc tcaccatgtg ggtgacggag gacgagcacc
     1021 ggcggctgct cgagcgctgc gacggcaggc agctggccgc gtggatgcgg cagacctgcc
     1081 tggacgaaaa gcctgcgcgt accggaaagc ttccctcact ctcgccggcg ctgctgcgcc
     1141 agcttgccgg catgggcaac aacctgaacc agatagcccg ccgcgttaat gccggcggcg
     1201 gtacgggcca tgacagggtg cagattgtgg cggcgctgat ggccattgat gccggacttg
     1261 agcggctgcg gcacgccgtt cttgagaaag gaacggacga tgatcgttaa atttcatccg
     1321 cgcggccgcg gcggtggcgc cgggcctgtg gattatctgc tgggtaaaga ccggcagcgt
     1381 gacggtgcca ccgtcctgca ggggaagccg gaagaagtcc gggagctgat tgacgcatcg
     1441 ccttacgtga agaaatacac ctccggcgtc ctgtcgtttg cggaggccga tctgcctccc
     1501 ggccagcggg aaaagctgat ggccagcttc gagcgggttc tgatgcccgg actcgataaa
     1561 gatcagtaca gcattctgtg ggtggagcac gcggacaagg ggcgtctgga gctgaacttc
     1621 ctgatcccca ataccgagct gctgactggc aggcgactcc agccatacta cgaccgcgcc
     1681 gaccggccgc gcatcgatgc ctggcagacc atcgtgaacg gcagactggg gctgcacgac
     1741 ccgaacgcgc cggaaaaccg cagggcgctg gtcacgccgt ccggcctgcc gaaaacgaag
     1801 caggaggccg cagaagccat tacccggggg ctgctgagct tagcctcgtc cggggagctg
     1861 aaaagccgcc aggacgtcac tgacactctt gaaggtgcag gttttgaggt ggtgcgcacc
     1921 acgaaaagca gtatcagcat cgccgacccg gacggggggc gaaacatccg actgaaggga
     1981 gccatctatg agcaatcttt ttacggcctc gagggacttc gaggagaaat tgaaagcgca
     2041 gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgagc agtctgtcgg
     2101 agcggaactg agcgcaagcg cgaggagaat cagcgacgcc atcctcgccc acgagctgag
     2161 tatgagcgag gcgatggaga aaaaccggcg gagcgtgctg ctgacggcag ggaggacatg
     2221 gctcaccatc ctgatggttt cagcgctgct gatcggcgcg agcgggagca ttctgtggtg
     2281 gcaggggcag cagataaccg acaattacac gacccttcgc cagcaggagg acgccctggc
     2341 gaagatgagc gcgaaaacat ggggtgtgag ctaccagagc gacagcaacg gacggttcct
     2401 ggtgctgccg aagggagcga aagccgaaac aggctggaca gtggacagcg ggaagcggaa
     2461 cgcggtgaag ctggtacagg agtagagcag aatgacagag ctggaaaagc agttgctgag
     2521 cgcattagag cagctacagc aggactactc gcaaaggctg gacgaatggg agagcgcctt
     2581 cgaggaatgg cggaggatgt ctgggcttat gcaacgggag aacgcgatgc tgaacgagcg
     2641 cgtatcgggc ttgagcaggc aggtgcagag tttgagcgag cagctgcgcc gcttgtccgg
     2701 gagctgaatg ccattcagca gaacaaggcg caggaaaagc tgcatatgaa agatatgaca
     2761 gctgagcggc aatctaaaaa aacatacggt gggccaacgt taggataatt ttcaatttcc
     2821 tgttgtgtaa tatcgtttcc tgttatattg tttttgagaa cttaaaattc acctcaggaa
     2881 atttgaaatg gtcaatgaat catctagaag gcttactaat tttacaactg tactacttct
     2941 tcttatcaaa gaatgtcgac tcgagagggg agttcatcag gctcaactag ctgaaaagat
     3001 aaataaaagc ccgagttcat ttgcaaaaat cgagactggt aagagccctc ttacgatgga
     3061 ggtctttctt gcatgttgca gtacgttgat gatttcacca tcagctgtaa tgtctactgc
     3121 ggagcgttat ggaaacctgt taaccaataa tggttggatc ttgcttaaca gttctctgga
     3181 agagaatgat gatgaactta tgaaagaatc ccaggagtac tattcatcac ctggttataa
     3241 aagcaggcta aatatgactt cagctcttaa tgggccgttc tattatccaa atggtaatgt
     3301 tgatgggtta gctgttttcc tttttgctat atctacccac tttaaaaatc aacagcttga
     3361 agctcaaaac tctcttttat ctggatggaa ttaaagtccg ataaatagag tgtatggagt
     3421 taattttccc taagatggct ccacatacac atttcagcat agttatgcat tcggttaaaa
     3481 tttacctgtc gcgatcgggg gggtttctcg ggtggtttgt tacccgtttt acctgatagc
     3541 cgccccgttc gcggcgaacc tgtctggagg ggttcgggca acggatgtta tggtaaatgt
     3601 cgagattttg agttgttcag ctgacgattt tcgcatcaaa tttttgctgt atgtttaaac
     3661 atgattttaa catcaatgtc atctgagggt gttgttgcta atttcatcta cctgacaggt
     3721 aatttccttt taaatcaaga gcgaaagatc agcgaaaaag ctatgtttgc gtttgtggaa
     3781 tagggtagaa taggggattc tgatgatcgt gaggttgtca gtaaaaacga ggcagctatc
     3841 agatcttcgg cctgagaaac taaggattct gatagctgcc ggtgactcag ttcgcgaaaa
     3901 ctgaatctta cctgatcaac aaacgcccga taagggcgtt tgtcaagtgg tgctgcatta
     3961 tgtagtaact tggtacctac ttgccaggca tttttcacag ttttttgatg tgcgaaggct
     4021 catccggaca ccgtcacctg ttatctaatc cttataaaca ggtgctataa atgcaaaaag
     4081 taactgatct gtatccccct gaaattgctc gtcataatct tcaaaatcac ttcgctggca
     4141 atactgttat gctggagtta atccagaaac taaataaaac cagcttatgt acttttgcgg
     4201 cgctctgtga cggcaacgtc gtgaccactt ccggctataa cattacggcc gatctgtgtg
     4261 ttaaccgtgc gtcagccgtg gtccattcgc ttaagcaaaa atatctgccg gttacggcca
     4321 taacgatttc gactaaggcc gatgtaggtg gtgcggtaaa gcaggcagct ttctgtatcg
     4381 atgaggacga tcttgcaagg ctgaaatcag agccggaaaa gatgatgaaa gagtgcgaac
     4441 gtaacctgaa cagccagaag cagaccaatg ctcagaagga aatgagcagg ttgtacaagg
     4501 agtttggtga agatggaatt ctggctctat tgcgcaacgt ttcccgttcg aacgggaatg
     4561 gcacgccgcc gccaagcggg cagcctgcga gctgatgatc cttagctaac tggaaatact
     4621 aaagcccgct ttgcgggctt tttcatacgt ttaatacaaa ggaataatta tttctcagac
     4681 tttacaaaaa aactattaag gccgcttttg taattcactg cctacagatc aacctttttc
     4741 acgacctcat atattgctgc gttattgatg ttgcgatttt tgaattcaat tacgaactca
     4801 ccttctgttg ctttgtccca cagttcgttt acaaatgtgc gaaagcgacg acctaaagac
     4861 ttcctggttg atgggctaag cctgttccaa gaatcatcta acgctttgaa atataattca
     4921 tttgctttga agcttttgtt ttcttttcta ttttttattg caaaagaaac catttgaagt
     4981 attgcttggt taatttcttc atcgtttagc gttttggttt ttttgctgtt aactgcgtca
     5041 ttcagcaatg atgcgaccgt ttcttcaaaa ctcatcccat cttcgttttc atttgcatgg
     5101 ttaagaatgt catgcacgat agactcttct atatcaataa caactctcat ataaacccct
     5161 tgtttttggt tcttttgtgt tgtttttaaa tatagcgcca aaaattaaac atgtaaacat
     5221 gttttttgtt gtttcttgtt gtttcttgtt gtttgtggtt gttttctctt tgtggggcgt
     5281 caggggagtt gaaggcttga agaggtcgaa gatacttgtt acaaaatcgc cctaatgcag
     5341 attaggcatt agagcgcttt tctttgacat tgtgagttgg ttcgtcaaca atcgacagta
     5401 ggaatctaat ttacagaggt gtgatatgta taaagatatt ggaccaggcc caatcgtatt
     5461 ctaacgacca ccttgctttg cttggaggag ttgcagcaat gtgaacccct ccatgtagct
     5521 ttcttttaag tctcccagca acccctcaag catagctatt tgtctgtcct tagatatttc
     5581 cttgtatctg tctgaattga tggtttctat aaacttgata agagaccgag tattgctata
     5641 aaaacctacg atttcagaaa ctaaatcttt cggcattagc catttctcat ctttccaatc
     5701 aatgtgatct tcaaggcctt cgttataact aacccacggc atatacgtat cttcaagttt
     5761 ggctttgttg atagcgtgtt gaaatggaac aagaaattta tctattttct ttatgcgtgc
     5821 gttaacgtcc acaatgagaa tcttaacgag gtttttcttt gtcctattgg taatccaaaa
     5881 atctctgatt gttggagcta gaaatgccaa aagggctgct atcactgcgc ctatgactct
     5941 gggatcattc attaaagttg agcttggcgg agccattcca ggagat
//
