LOCUS       NZ_CP126814             2495 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain 22KM0858 plasmid pKM58_3, complete
            sequence.
ACCESSION   NZ_CP126814
VERSION     NZ_CP126814.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394401
            Assembly: GCF_030848145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848145.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:56:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,941
            CDSs (total)                      :: 4,825
            Genes (coding)                    :: 4,737
            CDSs (with protein)               :: 4,737
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 33 of 88
            Pseudo Genes (incomplete)         :: 61 of 88
            Pseudo Genes (internal stop)      :: 13 of 88
            Pseudo Genes (multiple problems)  :: 16 of 88
            Pseudo Genes (short protein)      :: 1 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126814.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="22KM0858"
                     /isolation_source="swab hip joint"
                     /host="Dog"
                     /db_xref="taxon:158836"
                     /plasmid="pKM58_3"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022-03-28"
     gene            48..239
                     /locus_tag="QPR77_RS24690"
                     /old_locus_tag="QPR77_24680"
     CDS             48..239
                     /locus_tag="QPR77_RS24690"
                     /old_locus_tag="QPR77_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(530..715)
                     /locus_tag="QPR77_RS24695"
                     /old_locus_tag="QPR77_24685"
     CDS             complement(530..715)
                     /locus_tag="QPR77_RS24695"
                     /old_locus_tag="QPR77_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1081..1308)
                     /locus_tag="QPR77_RS24700"
                     /old_locus_tag="QPR77_24690"
     CDS             complement(1081..1308)
                     /locus_tag="QPR77_RS24700"
                     /old_locus_tag="QPR77_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          396..483
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1724..2252)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ccgttaacag taggtaagcc atgtttggtt tttcttccgg agccctgatg actcttagtg
       61 atgatgagcg acacctgctt gtgtccgttg tttcggtctg gctacggcgt gccggagggg
      121 atgccggagc catgatgctg gatgcatacc ggcagatttt gtctgaaacc gaaccggccg
      181 taaggactgt catgctcgaa tttcttgaat ccgttcgcat tcattacatt tcgtcctgag
      241 cattttctgt atttctgtcc atacttaaac gaggcggccg tcacggacgg tggtcgtcgt
      301 tccatgatgc tgttatggac gagtgagagt aatctaatct aacttgattg agttccgggc
      361 gtgccgcccg gttttttttg ctgtttcccg gtgtccgttc cgggacagga tgtgcatttg
      421 taataccgcg cacgcgccgg catcacaaat accctggtca gggctgcgcc ccgacacccc
      481 gccatcgcct cacggccgcg cactgaattg cgcacccgtt caccgatttt cacgcatttt
      541 cctcgcctat ctgtgcgttc agcatggcgt aaagcgcttc ggcctgatcg tgcaggtgct
      601 cacagcagcc ggcagcctca tccagatcca tctgctcgag ccgctcaagg aggagaagtg
      661 tctgtgcgcg gataaatttg gccatattga gggcggcgga ttcctgtctg gtcactggtg
      721 attttccccg tctggtgatg atttttgcgt gaaaagagca tggcgtcatt ctggtgcata
      781 gtcatgcatg ccgttaaaat ttaacaggcg cgtttctgac gggttccggg gtggtttgtt
      841 gtggtttttg tcatggtttc gtcaggaacg cgctgaacgc gtctcaggcg gtgcgggcaa
      901 cggatgttat ggtaaattct tgggggcttg attataaaat accgcacttc ctcttatgtc
      961 ttgtgctttt tagacaaccc tgaaagcttt tagctttggt gatgcaattt gctgggattt
     1021 tggcggagaa ccacaggtaa agaaaaaggc cacattagcg gcctttttcg gagaagatgc
     1081 tcagtgcgga atttcggaga cttctttaac gtcggtttta ttgatctgct gtttgacacc
     1141 attcgcgtct gtatagccaa gcaggcctga gtctgattct ttaggtttgc cgtcgctgac
     1201 aatagtgcga ccgtcattgg tgtgtatggc atagcttgtg cgcgtgcagc cggtgagggc
     1261 tgaaagcgca actgccgctg cgaacactga aacaaaaatc tttttcaaag ccagctcctt
     1321 ttatccatat cagttttggt cactaaccat ctaactatag gtcatttttt atgtgaaaag
     1381 aggcttgatg gtgggctgta tatcgagtta ggcgcgaggt gctatggtca aagtgtggtg
     1441 tcaggcgggg aggaggctta tatgagatgt aatccggccg atacatatac caagatgatt
     1501 taagtattcc tctttgtgag gttggatgtc taatttataa aggatcttct tgagatcctt
     1561 tttttccgcg cgtaatctct tgccctgtaa acgaaaaaac caccctggca ggtggttttt
     1621 cgaaggttag gtaatcctgg cagatcccct aaccgtggta acagtcttgt gcgagacatg
     1681 tcaccaaatt tgtcctttca gtgtagcctc actaaggccg ccacttcaag aactcttgag
     1741 acatctctcg cacatcctgt ttgccaatgg ccgttgccaa tggcgattag tcgtgtcttt
     1801 ccgggttgga ctcaagttga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
     1861 gttcgtgcat acagtccatc ctggagcgaa ctgccttccc ggaactgagt gtcaggcgtg
     1921 gaatgagaaa ccgcggccat aacagcggag tgacaccggt aaaccgaaag gcaggaatgc
     1981 gggggagcac gagggagcca ccagggggaa acgcctggta tctttaagcc gcatcgggtt
     2041 tcgccaccac tgatttgagc gtcagattct gtgatgcttg tcaggggggc ggagcctatg
     2101 gaaaaacggc tttggcgcgg ccttatgctt tcttcgttaa gtatcttcct ggcatccccc
     2161 aggaaatttc tgatccatcc gtaagcccgt cccgctcgcc gcagccgaac gaccgagcgg
     2221 agcgagtcag tgagcgagga agcggaatat attctgtatc acattttctc ctgacgcgtt
     2281 ttctttcact ttctgcgcct gtcttatgtg gcattaatgc tatgtgttac tgccatgcta
     2341 catcttaagc cagtatacac tccgctagtg ctccgtgact ggtccggcgc tgcgcccgga
     2401 acccgcctgt accggttcag cagccgttcc ggcctgactg caattttttt tttttcatcc
     2461 ctgcccgcta ccctgtaaac ctttcttctg cgttg
//
