LOCUS       NZ_CP126810             2495 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain 22KM1314 plasmid pKM58_3, complete
            sequence.
ACCESSION   NZ_CP126810
VERSION     NZ_CP126810.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394402
            Assembly: GCF_030848125.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848125.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:05:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,757
            CDSs (total)                      :: 4,641
            Genes (coding)                    :: 4,561
            CDSs (with protein)               :: 4,561
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 52 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 16 of 80
            Pseudo Genes (short protein)      :: 1 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126810.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="22KM1314"
                     /isolation_source="urine"
                     /host="Cat"
                     /db_xref="taxon:158836"
                     /plasmid="pKM58_3"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022-05-23"
     gene            complement(join(2424..2495,1..12))
                     /locus_tag="QPR76_RS23765"
                     /old_locus_tag="QPR76_23750"
     CDS             complement(join(2424..2495,1..12))
                     /locus_tag="QPR76_RS23765"
                     /old_locus_tag="QPR76_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032623649.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHD"
                     /name="hypothetical protein"
     gene            658..876
                     /locus_tag="QPR76_RS23770"
                     /old_locus_tag="QPR76_23755"
     CDS             658..876
                     /locus_tag="QPR76_RS23770"
                     /old_locus_tag="QPR76_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1167..1352)
                     /locus_tag="QPR76_RS23775"
                     /old_locus_tag="QPR76_23760"
     CDS             complement(1167..1352)
                     /locus_tag="QPR76_RS23775"
                     /old_locus_tag="QPR76_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1718..1945)
                     /locus_tag="QPR76_RS23780"
                     /old_locus_tag="QPR76_23765"
     CDS             complement(1718..1945)
                     /locus_tag="QPR76_RS23780"
                     /old_locus_tag="QPR76_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1033..1120
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..266)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.418"
                     /coverage="84.0"
                     /evalue="1.15e-98"
     Typing          complement(2399..2495)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.735"
                     /coverage="93.0"
                     /evalue="1.1e-21"
ORIGIN
        1 gggttcgtgc atacagtcca tcctggagcg aactgccttc ccggaactga gtgtcaggcg
       61 tggaatgaga aaccgcggcc ataacagcgg agtgacaccg gtaaaccgaa aggcaggaat
      121 gcgggggagc acgagggagc caccaggggg aaacgcctgg tatctttaag ccgcatcggg
      181 tttcgccacc actgatttga gcgtcagatt ctgtgatgct tgtcaggggg gcggagccta
      241 tggaaaaacg gctttggcgc ggccttatgc tttcttcgtt aagtatcttc ctggcatccc
      301 ccaggaaatt tctgatccat ccgtaagccc gtcccgctcg ccgcagccga acgaccgagc
      361 ggagcgagtc agtgagcgag gaagcggaat atattctgta tcacattttc tcctgacgcg
      421 ttttctttca ctttctgcgc ctgtcttatg tggcattaat gctatgtgtt actgccatgc
      481 tacatcttaa gccagtatac actccgctag tgctccgtga ctggtccggc gctgcgcccg
      541 gaacccgcct gtaccggttc agcagccgtt ccggcctgac tgcaattttt tttttttcat
      601 ccctgcccgc taccctgtaa acctttcttc tgcgttgccg ttaacagtag gtaagccatg
      661 tttggttttt cttccggagc cctgatgact cttagtgatg atgagcgaca cctgcttgtg
      721 tccgttgttt cggtctggct acggcgtgcc ggaggggatg ccggagccat gatgctggat
      781 gcataccggc agattttgtc tgaaaccgaa ccggccgtaa ggactgtcat gctcgaattt
      841 cttgaatccg ttcgcattca ttacatttcg tcctgagcat tttctgtatt tctgtccata
      901 cttaaacgag gcggccgtca cggacggtgg tcgtcgttcc atgatgctgt tatggacgag
      961 tgagagtaat ctaatctaac ttgattgagt tccgggcgtg ccgcccggtt ttttttgctg
     1021 tttcccggtg tccgttccgg gacaggatgt gcatttgtaa taccgcgcac gcgccggcat
     1081 cacaaatacc ctggtcaggg ctgcgccccg acaccccgcc atcgcctcac ggccgcgcac
     1141 tgaattgcgc acccgttcac cgattttcac gcattttcct cgcctatctg tgcgttcagc
     1201 atggcgtaaa gcgcttcggc ctgatcgtgc aggtgctcac agcagccggc agcctcatcc
     1261 agatccatct gctcgagccg ctcaaggagg agaagtgtct gtgcgcggat aaatttggcc
     1321 atattgaggg cggcggattc ctgtctggtc actggtgatt ttccccgtct ggtgatgatt
     1381 tttgcgtgaa aagagcatgg cgtcattctg gtgcatagtc atgcatgccg ttaaaattta
     1441 acaggcgcgt ttctgacggg ttccggggtg gtttgttgtg gtttttgtca tggtttcgtc
     1501 aggaacgcgc tgaacgcgtc tcaggcggtg cgggcaacgg atgttatggt aaattcttgg
     1561 gggcttgatt ataaaatacc gcacttcctc ttatgtcttg tgctttttag acaaccctga
     1621 aagcttttag ctttggtgat gcaatttgct gggattttgg cggagaacca caggtaaaga
     1681 aaaaggccac attagcggcc tttttcggag aagatgctca gtgcggaatt tcggagactt
     1741 ctttaacgtc ggttttattg atctgctgtt tgacaccatt cgcgtctgta tagccaagca
     1801 ggcctgagtc tgattcttta ggtttgccgt cgctgacaat agtgcgaccg tcattggtgt
     1861 gtatggcata gcttgtgcgc gtgcagccgg tgagggctga aagcgcaact gccgctgcga
     1921 acactgaaac aaaaatcttt ttcaaagcca gctcctttta tccatatcag ttttggtcac
     1981 taaccatcta actataggtc attttttatg tgaaaagagg cttgatggtg ggctgtatat
     2041 cgagttaggc gcgaggtgct atggtcaaag tgtggtgtca ggcggggagg aggcttatat
     2101 gagatgtaat ccggccgata catataccaa gatgatttaa gtattcctct ttgtgaggtt
     2161 ggatgtctaa tttataaagg atcttcttga gatccttttt ttccgcgcgt aatctcttgc
     2221 cctgtaaacg aaaaaaccac cctggcaggt ggtttttcga aggttaggta atcctggcag
     2281 atcccctaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatttgt cctttcagtg
     2341 tagcctcact aaggccgcca cttcaagaac tcttgagaca tctctcgcac atcctgtttg
     2401 ccaatggccg ttgccaatgg cgattagtcg tgtctttccg ggttggactc aagttgatag
     2461 ttaccggata aggcgcagcg gtcggactga acggg
//
