LOCUS       NZ_CP126803             4853 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_15 plasmid pEh15_5, complete
            sequence.
ACCESSION   NZ_CP126803
VERSION     NZ_CP126803.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394404
            Assembly: GCF_030848085.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848085.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:24:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,415
            CDSs (total)                      :: 5,298
            Genes (coding)                    :: 5,168
            CDSs (with protein)               :: 5,168
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 36 of 130
            Pseudo Genes (incomplete)         :: 100 of 130
            Pseudo Genes (internal stop)      :: 19 of 130
            Pseudo Genes (multiple problems)  :: 22 of 130
            Pseudo Genes (short protein)      :: 1 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126803.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_15"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh15_5"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-10-31"
     gene            898..1221
                     /locus_tag="QPR56_RS27040"
                     /old_locus_tag="QPR56_27040"
     CDS             898..1221
                     /locus_tag="QPR56_RS27040"
                     /old_locus_tag="QPR56_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015060398.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1430..>1864
                     /locus_tag="QPR56_RS27045"
                     /old_locus_tag="QPR56_27045"
                     /pseudo=""
     CDS             1430..>1864
                     /locus_tag="QPR56_RS27045"
                     /old_locus_tag="QPR56_27045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1899..2390
                     /locus_tag="QPR56_RS27050"
                     /old_locus_tag="QPR56_27050"
     CDS             1899..2390
                     /locus_tag="QPR56_RS27050"
                     /old_locus_tag="QPR56_27050"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2397..2612
                     /locus_tag="QPR56_RS27055"
                     /old_locus_tag="QPR56_27055"
     CDS             2397..2612
                     /locus_tag="QPR56_RS27055"
                     /old_locus_tag="QPR56_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3076..3567
                     /locus_tag="QPR56_RS27060"
                     /old_locus_tag="QPR56_27060"
     CDS             3076..3567
                     /locus_tag="QPR56_RS27060"
                     /old_locus_tag="QPR56_27060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127627441.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIQP
                     "
                     /name="hypothetical protein"
     gene            complement(3624..4052)
                     /locus_tag="QPR56_RS27065"
                     /old_locus_tag="QPR56_27065"
     CDS             complement(3624..4052)
                     /locus_tag="QPR56_RS27065"
                     /old_locus_tag="QPR56_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_306675462.1"
                     /translation="MSYAIEKKWKVGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            4337..4765
                     /locus_tag="QPR56_RS27070"
                     /old_locus_tag="QPR56_27070"
     CDS             4337..4765
                     /locus_tag="QPR56_RS27070"
                     /old_locus_tag="QPR56_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          610..783
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(140..686)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.455"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          899..1215
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="1.6e-61"
     Typing          1212..2710
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.287"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggctttttcg aaggttcgca agagttgacg ctcttttgaa ccgcggtaac tggcttggag
       61 gagcacagtc accaaaactg tcctttcagt gtagccttat cgcacccgaa acttcaagac
      121 tctcctcctc taaacctgtt accagtggct gctgccagtg gcgctttgtc gtgtttttcc
      181 ggattggact caagttgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      241 tcgtgcatac agtccagctt ggagcgaact gccttcccgg aactgagtgt caggcgtgga
      301 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
      361 agagcgcacg agggagccac cagggggaaa cgcctggtat ctttaagtcc tgtcgggttt
      421 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
      481 aaaaacggct tcgccgcggc cttatcgttt ccctgttaag tttcttcctg gcatctacca
      541 ggaaatttcc gccccattcg taagcccgca ccgctcgccg cagccgaacg accgagcgca
      601 gcgagtcagt gagcgaggaa gcggaatata tcctgtatcg catattctgc ttacgcgccg
      661 gtgccgcctt ttttctcctg ccacatgaag cacttcactg attcccgcac ccgtgccaac
      721 atagtaagcc agtatacact ccgctagcgc tgcgtgactg gttcagggct gcgccccgaa
      781 acccgctaaa agccactgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg
      841 ctttcccgcc cgtcccggcg ttatcaggag gccggtttgt cagagaaacg gaacaagatg
      901 ctcaccatgt gggtgacgga ggacgagcac cggcggctgc ttgagcgctg cgacggcagg
      961 cagctggcag cgtggatgcg gcagacctgc ctggacgaaa agccggcacg ttccggaaag
     1021 ctcccctcac tctcgccggc gctgctgcgc cagcttgccg gtatgggcaa taacctgaac
     1081 cagatagccc gccgcgttaa cgccggcggc ggcactggcc atgacagggt gcagattgtg
     1141 gcggcgctga tggccatcga tgccggactt gagcggctgc ggcacgccgt tcttgagaaa
     1201 ggaacggacg atgatcgtta aatttcatcc acgcggtcgc ggcggtggcg ccgggccggt
     1261 ggactacctg ctgggtaaag accggcagcg tgatggtgcc accgtcctgc aggggaagcc
     1321 ggaagaagtc cgggagctga ttgacgcgtc gccttacgtg aagaaataca cttccggcgt
     1381 cctgtcgttt gcggaggccg atctgcctcc cggccagcgg gaaaaactga tggccagctt
     1441 cgagcgggtt ctgatgcccg gacttgataa agatcagtac agcattctgt gggtggagca
     1501 cgcggacaag gggcgtctgg agctgaactt tctgatcccc aataccgagc tgctgaccgg
     1561 caggcggctc cagccgtact acgaccgcgc cgaccggccg cgcatcgatg cctggcagac
     1621 catcgtgaac ggcagactgg ggctgcatga cccgaacgcg ccggaaaacc gccgggcgct
     1681 ggtcacgccg tccggactgc cgaaaacgaa gcaggaggcc gcagaagcca tcacgcgtgg
     1741 actgctgacc cttgcttcgt ccggggagct gaaaagccgt caggatgtca ctgaggcgct
     1801 ggaacggtca ggttttgagg tggtgcgcac cacgaaaagc agtatcagca ttgccgaccc
     1861 ggacgggggg cgaaacatcc gactgaaggg agccatctat gagcagtctt ttaacgctgg
     1921 cgaaggactt agagcagcaa tcgaaagcgc agaagcagag taccggcgag atgctgaaag
     1981 ccgcattcag cgagcacgag cagtctgtca gagcggaact gagcgcaagc gcgaggagaa
     2041 tcagcgacgc catcctcgcc cacgagcaga gtatgagcga ggcgatggag aaaaaccggc
     2101 ggagcgtact gcggactgcc gggcggacat ggctcaccat cctgatggtg tcagcgctgc
     2161 tgatcggcac gagcgggagc attctgtggt ggcaggggca gcagataacc gacaattaca
     2221 cgcatcttcg tcagcaggaa gataccctgg cgaaaatgac cgcccggacg tggggagtgc
     2281 gctatcagga gagcagcgac ggccggaggt ttcttatcct gccgccgggg atgcaaaccg
     2341 aagccatccc ttacgacggg acgacatgga tccgcctgaa acaggagtag cggaagatga
     2401 cagagctgga aaagcagttg ctgagcgcat tagagcagct acagcaggac tactcgcaaa
     2461 ggctggacga atgggagagc gccttcgcgg aatggcggag catgtgtggg cttatgcaac
     2521 gggagaacgc ggtgctgaac gagcgcgtgt cggacttgag cacgcaggtg ctgagtttaa
     2581 gcgagcagct gcgccgtttg tccgggaact gaatgccatc gaggagaagg ctgtactgga
     2641 gtatcagcgt cagaacgagc tggagctgac gctccgtcag aagcagaaga gttttgatgg
     2701 tccgaagatg aggtgacagc ccgcacgaag aagggctcat cgcgttcctg ctaaataggc
     2761 tgactaccgg aatggtagtg acttatcccg gatgaagtga tgaaaattct taacgttatt
     2821 gaagccctgc tgaactgttt ttacctggtg atggagctgt gtgagttgcc ggagaggcat
     2881 ttgccggttt ttaaggcgtt cgcgctgatt ttagtgtgac tttacgcggc atgcatctgg
     2941 ctgcatgact atgcataagt gtaatatcca gcggctgcgt ttccggtgtt ttttcgtcat
     3001 gtctgtcgcc actttctcgc gtctggcacc ggaaacgcag ccaaccccgt ttcagctgtg
     3061 cgggcaacag atgttatggt aaatcaaccg tccgttgata aaggttctga taatacccat
     3121 gtaggtgatg ttgtttgctt tttatctaca cactgcctga tggagctatt tatgaatgct
     3181 gatgaagtta cacgaattct tgcgcaatcg agtcaaaatg attggattgt ggatgatgag
     3241 acaggggcgt ttacttataa aaatgattta gcactgcaca ttaagagagc tgattacgac
     3301 gactacaact cgtttaatga gccttgggct gttcgtcatc ctgatagtaa tgcgtgttca
     3361 gtcgagtaca ccgttcacta tggttcttcg tttgttgata gaaaaacctt ggtttccgtc
     3421 gatggtcatc gtgcaacctt gcctatgcct gcatccgcaa cggatctaaa cgtgtcatcg
     3481 gctgatgtga atttcgccaa aattgtaaac attggcgatc gcgttgatga atacattcaa
     3541 cgttctggtc ttacgattca gccctaattt aacgataagc ccggcctcca aatgcgggct
     3601 gttcctgcat gtaaccagca taactaatcg gcttttttcc ttactgtata tattggcttt
     3661 ttatgtcgcg gctttcttac ttgctcggac acagaaggac caattatgtc taaataatct
     3721 acaagctttg taaggtcgta tatgttgcgg ccaccatatc ggttgtcgct ttttgatgtc
     3781 ggttttttat ctaagagatt ggctttttct aggtcattaa tagccctttg caccgtcctt
     3841 actgacaccc ccatccgtat agccaggctc tccattgagg ggtgcggcca ctcctgtaga
     3901 ttccaccagt gtactatgag attcagcaag gtattgaatg ctacagggct caatttcttc
     3961 tcattttgta taaaaaacaa cacagtaggt actgcagtcc atcctaatga cagacctcca
     4021 accttccatt ttttttctat tgcgtaactc attgattatc ctgtaaatat tttttttgag
     4081 atatatataa tataagtatt catatgaata gggtgacacc agcgccatta ttacacaact
     4141 ataaaaggct aatctgagaa cagaaaaaca tttctatctg cattaaatac ggagttagtg
     4201 atatgaaaac caaacaacat catgatcttg taaaagatac tgaaatattt ggtgtatcct
     4261 tgtctctgtt gatttccaga gcgaatgaga gatctgttac aatgccatct ggtctaagta
     4321 gggagcagag aagagcatgg gcagcggatc aagcacgcaa aatccacaat tgaatgttgt
     4381 ctctttctct gagacattca atctttatta cacatcccgc ttttctcaaa aaacactcga
     4441 taaaatcgat gacttcatcg aacatttcga gaagtacaat ctttttggcg atgccttaaa
     4501 aggccatccg gcttggattg gcaaggtatc gccatcttgg aacgtgcctg acagttacga
     4561 aaacaaagaa gctattgaag aacatgccag ggctaataaa ttgtggcatg cccatatagg
     4621 cgatcccgtt ttcatcgata catttcacgg gaagtacaaa gtctctgatt gggtcatcca
     4681 cttccaacgt ctagcgccga accatataaa actgttagag cttgggtatc atgaccctat
     4741 gcaactcccc caaaatttcg aatgatctct tgttcggatg aatgatccct tgagattcgt
     4801 tgaccctcgt cgtaatctct tgcggtgaaa acgaaaaaac cacccggcaa ggt
//
