LOCUS       NZ_CP126797             3223 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_19 plasmid pEh19_5, complete
            sequence.
ACCESSION   NZ_CP126797
VERSION     NZ_CP126797.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394408
            Assembly: GCF_030848045.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848045.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:08:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,760
            CDSs (total)                      :: 4,644
            Genes (coding)                    :: 4,562
            CDSs (with protein)               :: 4,562
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 25 of 82
            Pseudo Genes (incomplete)         :: 51 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            Pseudo Genes (short protein)      :: 1 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126797.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_19"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh19_5"
                     /geo_loc_name="Switzerland"
                     /collection_date="2021-07-14"
     gene            199..684
                     /locus_tag="QPR73_RS23780"
                     /old_locus_tag="QPR73_23745"
     CDS             199..684
                     /locus_tag="QPR73_RS23780"
                     /old_locus_tag="QPR73_23745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            688..903
                     /locus_tag="QPR73_RS23785"
                     /old_locus_tag="QPR73_23750"
     CDS             688..903
                     /locus_tag="QPR73_RS23785"
                     /old_locus_tag="QPR73_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1071..1403
                     /locus_tag="QPR73_RS23790"
                     /old_locus_tag="QPR73_23755"
     CDS             1071..1403
                     /locus_tag="QPR73_RS23790"
                     /old_locus_tag="QPR73_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1612..1782)
                     /locus_tag="QPR73_RS23795"
                     /old_locus_tag="QPR73_23760"
     CDS             complement(1612..1782)
                     /locus_tag="QPR73_RS23795"
                     /old_locus_tag="QPR73_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2421..2744
                     /locus_tag="QPR73_RS23800"
                     /old_locus_tag="QPR73_23765"
     CDS             2421..2744
                     /locus_tag="QPR73_RS23800"
                     /old_locus_tag="QPR73_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2134..2325
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1671..2151)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..1001
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.149"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2735..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.319"
                     /coverage="99.0"
                     /evalue="7.15e-101"
ORIGIN
        1 cggaagcgaa gcaggaagcc gcccaggcga ttacgagcgg cctgcttgcc cttgcctcat
       61 ccggggagct gaaaacgcgt caggacgtca ctgaggcgct ggaaagcgca ggttttgagg
      121 tggtgcgcac cacaaaaagc agcatcagca ttgccgaccc ggacgggggg cgaaacatcc
      181 gactgaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa
      241 tcgaaagcgc agcagcagac taccggcgag atgctgaaag ccgcattcag cgagcacgag
      301 aagtctgtca gagcggaact gagcgaaagc gagaagagaa tcagcgccgc catcctcgac
      361 cacgaccgga agctgtcctc agccatgagc cagcgcacga aagggatgct gcgcatggtc
      421 agccagacgt ggctgaccat cgttctggtc tccgcgctgc tgatagcgtc gagcgcgggc
      481 attctgtggt ggcaggggca gcagatactc gagaactata cgaccatccg ggagcagaag
      541 agcacgcagg ccatgctgtc agagaggaac agcggcgtac agctctcgac ctgcggcgag
      601 cagagacgcc gctgcgtgag ggtgaacccg gaagcgggac agttcggaga ggactcgagc
      661 tggatgatac tggcggggaa atagcacatg acggagctgg aaaaacagtt gctgagcgca
      721 ttagagcagc tacagcagga ctactcgaaa aggctggacg agtgggagaa cgccttcgcg
      781 gaatggcgga cgatgtctgg tcttattcaa cgggagaacg cggcgctgaa cgagcgcgtc
      841 acggtcttga gcaggcaggt gcagagtttg agcgagcagc tgcgccggtt gtcgaaaggc
      901 tgaacgcgat agaaactcac cggcagcagg agcgggcggt gcagcaccag aaagcgctgg
      961 agctggaacg atcgcagtgg cagcatcatg gtccttcgct atgataagcc gggttctatt
     1021 aattagttgt tatgtaaaga tatttaaatt tttcaacatg aggtattttt atgaatacat
     1081 atattttcct gggaggggct attgtggctg aagtcatagg caccaccttt atgaaatatt
     1141 cagaaggatt tacacgcctg tggccgtctg taggaactgc aataagttat tgcgctgcct
     1201 tttatctgct ttcccaaaca ttggaaaata tacctaccgg tattgcttac gcaatttggt
     1261 ctggtgcggg aattgtacta ataagcctag ctggttggtt ggttagcggt caaaaacttg
     1321 attggcccgc aattgcagga atgttcctta tttgcgttgg agttttggtt attaatattt
     1381 tttcaaaatc ttctgcgcat taacttttta ccgaagcata ttggccaaag caaaaggatt
     1441 tccttgagat cctttctttc tgcgcgtaat ctttttccct gtaaacaaaa aaaccaccgc
     1501 taccaacggt ggttcgtttg ccggaattca gagctaccaa ctctttgttc cgatggtaac
     1561 tggctttcac ggagcgcaga tacctaaact gtcctttcag tgtagccgca ctcaggcttt
     1621 cacttcaaga actctgtagg cattctctac aatctctgct ccgtgaaccc agttaccaat
     1681 ggctaccgcc actggcgatt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc
     1741 ggaacgggcg tggtagtcgg actgaacggg gggttcgtgc atacagtcca tcctggagcg
     1801 aactgcctac ccggaaccga gtttcaggcg tggaatgaga aaccgcggcc ataacagcgg
     1861 aatgacaccg gtaaaccgaa aggcaggaat gcgggggagc acgagggagc caccaggggg
     1921 aaacgcctgg tatctttaag ccgcatcggg tttcgccacc gctgatttga gcgtcatttt
     1981 cgtgatactc gtcagggggg cggagcctat ggaaaaacgg caactccgca tccttttctt
     2041 ggcctttcct gcttcgagcg gcatttgctt atcccgtaat ctgtgagctg gtaacgcccg
     2101 ccgcagtctc acggcaggag cgtagcgacc gagtgagcga ggaggcgtca tttcatcacc
     2161 acctgcattt tgcacttacg cccggcagcc gcatttttcc cctgtcacat gaagcacttt
     2221 cataaattgc cactccgtca taacatgttt agccagtata cactccgcta gcgctacgtg
     2281 actggttcag ggctgcgccc cgaaacccgc taaaaccact gccgcgcctg cggcttgtcc
     2341 aacaccgtgc cgaccgggaa aagatttccc gtccgtcccg gcgttatcag gaggccggat
     2401 tggcagacaa acgcagcaaa atgctcacca tgtgggtgac tgaggacgag caccggcgtc
     2461 tgctggagcg ctgcgagggt aagcaactcg cggcctggat gcggcagacg tgcctggacg
     2521 agaagcccgc acgcgccggc aaacttccgt cgatctcgcc ggcgctgctt cgtcagcttg
     2581 ccggcatggg gaacaacctt aaccagattg cccgccaggt taacgccggt ggtggaagcg
     2641 gacacgaccg cgtgcagatt gtcgccgcgc tgatggccat cgatgccgga ctcgagcggt
     2701 tgcggcatgc cgtactggaa aagggtgctg atgatgatcg ttaagtttca tcctcggggg
     2761 cgcggcggcg gcggcgggcc ggtcgattat ctgctgggta aagatcgcca gcgcgacggc
     2821 gccagcgttc tgcaggggaa gccggatgaa gtccgggagc ttatcgacgc ctcgccctac
     2881 gccaaaaagt acacttccgg cgttctgtct tttgccgaac aggatttacc gcccggccag
     2941 cgcgaaaagc tgatggcgag tttcgagcgg gttctgatgc ccggactcga taaagaccag
     3001 tacagcgtgc tgtgggttga gcaccgggac aaggggcggc tggagctgaa cttcctgatc
     3061 ccgaacacgg agcttctgac cggcaagcgg ctacagccgt attacgaccg cgccgaccgt
     3121 ccgcgcatcg atgcctggca gaccatagtg aacggcaggc tggggctgca tgacccgaac
     3181 gcgccggaga accggcgggt gctggtctcg ccttccgcgc tgc
//
