LOCUS       NZ_CP126796             4995 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_19 plasmid pEh19_4, complete
            sequence.
ACCESSION   NZ_CP126796
VERSION     NZ_CP126796.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394408
            Assembly: GCF_030848045.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848045.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:08:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,760
            CDSs (total)                      :: 4,644
            Genes (coding)                    :: 4,562
            CDSs (with protein)               :: 4,562
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 25 of 82
            Pseudo Genes (incomplete)         :: 51 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            Pseudo Genes (short protein)      :: 1 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126796.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_19"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh19_4"
                     /geo_loc_name="Switzerland"
                     /collection_date="2021-07-14"
     gene            join(4892..4995,1..28)
                     /locus_tag="QPR73_RS23735"
                     /old_locus_tag="QPR73_23700"
     CDS             join(4892..4995,1..28)
                     /locus_tag="QPR73_RS23735"
                     /old_locus_tag="QPR73_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058645281.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPEKHDLTPLAAATGKGCTRDN"
                     /name="hypothetical protein"
     gene            378..686
                     /locus_tag="QPR73_RS23740"
                     /old_locus_tag="QPR73_23705"
     CDS             378..686
                     /locus_tag="QPR73_RS23740"
                     /old_locus_tag="QPR73_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341209.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_012477691.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNKWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSVDTLLEMVFKMGYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(702..1034)
                     /locus_tag="QPR73_RS23745"
                     /old_locus_tag="QPR73_23710"
     CDS             complement(702..1034)
                     /locus_tag="QPR73_RS23745"
                     /old_locus_tag="QPR73_23710"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_045332894.1"
                     /translation="MSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGVDP
                     GMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATTA
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1476..1679
                     /locus_tag="QPR73_RS23750"
                     /old_locus_tag="QPR73_23715"
     CDS             1476..1679
                     /locus_tag="QPR73_RS23750"
                     /old_locus_tag="QPR73_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071686691.1"
                     /translation="MAIYVGPHSKVGRNGGSFQECDINTTNHTVTRKYGRYCTIRDNEY
                     APPTSVPGRHWLQIFRTPDNTE"
                     /name="hypothetical protein"
     gene            1815..2042
                     /locus_tag="QPR73_RS23755"
                     /old_locus_tag="QPR73_23720"
     CDS             1815..2042
                     /locus_tag="QPR73_RS23755"
                     /old_locus_tag="QPR73_23720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053504437.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     gene            complement(2529..2744)
                     /locus_tag="QPR73_RS23760"
                     /old_locus_tag="QPR73_23725"
     CDS             complement(2529..2744)
                     /locus_tag="QPR73_RS23760"
                     /old_locus_tag="QPR73_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2751..3242)
                     /locus_tag="QPR73_RS23765"
                     /old_locus_tag="QPR73_23730"
     CDS             complement(2751..3242)
                     /locus_tag="QPR73_RS23765"
                     /old_locus_tag="QPR73_23730"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3277..3981)
                     /locus_tag="QPR73_RS23770"
                     /old_locus_tag="QPR73_23735"
                     /pseudo=""
     CDS             complement(<3277..3981)
                     /locus_tag="QPR73_RS23770"
                     /old_locus_tag="QPR73_23735"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3920..4243)
                     /locus_tag="QPR73_RS23775"
                     /old_locus_tag="QPR73_23740"
     CDS             complement(3920..4243)
                     /locus_tag="QPR73_RS23775"
                     /old_locus_tag="QPR73_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4357..4530)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          34..179
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.757"
                     /coverage="87.0"
                     /evalue="2.72e-25"
     Typing          4497..4995
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.8"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(2438..3930)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtaaaggg tgtacaagag ataactgatg cagagttctt gaagtatcag tccaactacg
       61 gctaaactgg aaggacgaat taggtggcag cggtcttgta caccctgtta ccacggttca
      121 taaacttgcc ggtttaatga accttcgaaa aaccaccttg ccgggtggtt ttttcgttta
      181 tgggcaaaga ctactctttg ctggattgat acgtcaacac aataagcttt acttaaaaga
      241 tcaaaatgtc tttacacttt ttcctcttga aagcaatatc attacttgta taatgccccg
      301 cccgtatata tatataagga gttccttgcc ggaactttta tcattgcgat acaggtaatc
      361 cgaagtggag ctgttctatg aaaaattcat ttgagctgat cagtacagac ccagtaaagt
      421 tcaatacatg gtcgatcaag tcaaaactca ttatcataat tagaaaacta atgattgata
      481 acaaatggtc gcaatcagaa gcggcagaaa agttaggtat cagtcaacca agagtaagca
      541 atatgctgaa cggaaaactg gacaaattct ccgtcgatac gctgttggaa atggttttca
      601 agatggggta caagctggat atggacttca ctccattaaa cacagagtcg cccctgacta
      661 tggttgtaaa aaaggcgatg gtgtaaccat cgccaatttg attagagcaa cttgtagcgc
      721 gaagctgtgg ttgccatgtt tttcttatct ggtccttggc acgttttcac aaacgcatgc
      781 agtaagtcca cctggccggg tgccttactg cggtatacac agcgataagc tgggcgacca
      841 ttgattctca actcaacaac gccggggccg atgaccgtca agtgtgcgat ggacatcccc
      901 ggatccaccc cgtagaacac cgaatccaat gcgtgcacga atgcagcctg aacccattct
      961 gggagtgatt ttaatgcctt gatagcctgg gcattaacag gtttgaacgt gtattcattt
     1021 tcgtcgtttg acataaacat cctcatctag gttattgatt tttagcaact taactattgt
     1081 taaaacgttt cgcatataaa ctccgattga ttgattgatt tatgagcata aaaaaaccac
     1141 accgccctat atttaggaaa gtgcgcgcat ggttattgct cgttgaacca acaattcatt
     1201 ggcaatctcg ccaacgatgc catagtatat aaattcttat atgtcatcaa ttcttattag
     1261 tgcttcaaac gtctgtttga ataaaaaaaa atcaatttga cttaggcgca gaagacctaa
     1321 cagttacatg tgttggtttt ccttacatca agatatgagg attttatcga cgcaatgtgg
     1381 aaagccaaca aagcacacta tgatattgat aaatatagaa attaataaaa tctacttaga
     1441 tactggtaat caactatctg acgaaggagc ttagaatggc tatttatgtt ggtcctcaca
     1501 gcaaagtagg tcgtaatggt ggttcattcc aggaatgtga tatcaacaca acgaatcata
     1561 cggtaacccg aaaatatggc cgctactgca caatccgcga taatgagtac gcccctccaa
     1621 ccagcgttcc aggccgccat tggctgcaaa ttttccgcac gccagataac acagagtaat
     1681 tcatcttctg cttagtaaag cggtaatatc ccttgtataa accggatagg attatcattg
     1741 taagatactg aaaacactga catttaatgc ttttcagtat cttagtattg agctgcataa
     1801 agcgaggcaa gcgaatgggt taccattaca aggaattaac cgtcatcgaa ttaactgcgg
     1861 aagagtttga gcttcataaa gagggatact ctttagagct tttaactaac atgagaaatg
     1921 aagaacacac caagtattgg gaagtttgcc cttcctgcga aggaaaaatg gatccagaag
     1981 aaaatcaagg gcatgaaggt tactgtgcca catgctggct cgataaccct gactggtggt
     2041 agttaataat ttaccataac atccgttgcc cgcaccgctg aaacggggtt ggctgcgttt
     2101 ccggtgccag acgcgagaaa gtggcgacag acataacgaa aaaacaccag aaacgcagcc
     2161 gctgaatatt acactggtgc atagtcatgc agccagatgc atggatgtta aagtcacact
     2221 aaaatccgcg ccaatgcctt aaaaaccgac aaatgcttct ccagccagtc ccacagctcc
     2281 gccgccagat acaggcagtt aagcaatgat tcaataagat ttaacagctt catttcccgc
     2341 tcctgaataa gtcgtgacct ttttgatcac gaacctattt agcgggagaa ataagagccc
     2401 ctcttcgtgc gggttgtcag ctgcctggat ggtttaaagt gtcggcccac ggtaggtttt
     2461 ctgcggctgg cgctccagct ccagtgtttt ctgatactca ttctccctct gccggagctc
     2521 gatggcattc agttcccgga caagcggcgc agctgctcgc ttaaactcag cacctgcgtg
     2581 ctcaagtccg acacgcgctc gttcagcatc gcgttctccc gttgcataag cccacacatg
     2641 ctccgccatt ccgcgaaggc gctctcccat tcgtccagcc tttgcgagta gtcctgctgt
     2701 agctgctcta atgcgctcag caactgcttt tccagctctg tcatcttccg ctactcctgt
     2761 ttcaggcgga tccatgtcgt cccgtcgtag gggatggctt cggtttgcat ccccggcggc
     2821 aggataagaa acctccggcc gtcgctgctc tcctgatagc gcactcccca cgtccgggcg
     2881 gtcattttcg ccagggtatc ttcctgctga cgaagatgcg tgtaattgtc ggttatctgc
     2941 tgcccctgcc accacagaat gctcccgctc gtgccgatca gcagcgctga caccatcagg
     3001 atggtgagcc atgtccgccc ggcagtccgc agtacgctcc gccggttttt ctccatcgcc
     3061 tcgctcatac tctgctcgtg ggcgaggatg gcgtcgctga ttctcctcgc gcttgcgctc
     3121 agttccgctc tgacagactg ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta
     3181 ctctgctgct gcgctttcga ttgctgctct aagtccttcg ccagcgttaa aagactgctc
     3241 atagatggct cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact
     3301 gcttttcgtg gtgcgcacca cctcaaaacc tgcaccttca agagtgtcag tgacatcctg
     3361 acggcttttc agctccccgg acgaagcaag ggtcagcagt ccccgggtaa tggcttctgc
     3421 ggcctcctgc ttcgttttcg gcagcccgga cggcgtgacc agcgcccggc ggttttccgg
     3481 cgcgttcggg tcgtgcagcc ccagtctgcc gttcacgata gtctgccagg catcgatgcg
     3541 cggccggtcg gcgcggtcgt agtaaggctg gagccgcctg ccggtcagca gctcggtatt
     3601 ggggatcaga aagttcagct ccagacgccc cttgtccgcg tgctccaccc acagaatgct
     3661 gtactgatct ttatcgagtc cgggcatcag aacccgctcg aagctggcta tcagtttttc
     3721 ccgctggccg ggaggcagat cggcctccgc aaacgacaga acgccggagg tgtatttctt
     3781 aacgtaaggc gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct gcaggacggt
     3841 ggcgccttca cgctgccgat ctttacccag cagatagtcc accggcccgg cgccaccgcc
     3901 gcggccgcgc ggatgaaatt taacgatcat cgtccgttcc tttctcaaga acggcgtgcc
     3961 gcagccgctc aaggccggca tcgatggcca tcagcgccgc cacaatctgt accctgtcat
     4021 ggccggtgcc gccgccggcg ttaacacggc gggctatctg gttcaggtta ttgcccatgc
     4081 cggcaagctg gcgcagcagc gccggcgaga tcgaggggag ctttccggaa cgtgccggct
     4141 tttcgtccag gcagatctgc cgcatccacg ccgccagctg cctgccgtca cagcgctcga
     4201 gcagccgccg gtgctcgtcc tccgtcaccc acatggtgag catcttgctc cgtttctctg
     4261 acaaaccggc ctcctgataa cgccgggacg ggcgggaaag cctttcccgg tcggcgcggg
     4321 gttggacaag ccgcaggcgc gtcaggcttt tggcgggttt cggggcgcag ccctgaacca
     4381 gtcacgtagc gctagcggag tgtatactgg cttactatgt tggcacgaat gcggatgtca
     4441 gtgaagtgct tcatgtggaa ggagaaaaaa tacggcaccg gcgcgtcagc agaatatgcg
     4501 atacaggata tattccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4561 gcgagcggaa atggcttact ggcgggacgg aaatgtcctg gaagatgcca ggaagaaact
     4621 tagcagggaa gcaataaggc cgcggcgaag ccgtttttcc ataggctccg cccccctgac
     4681 aagcatcaca aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     4741 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     4801 accggtgtca ctccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcggttc
     4861 cgggaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg
     4921 ctgcgcctgt tccggtaact atcgacttga gtccaacccg gaaaagcacg acttaacgcc
     4981 actggcggca gccac
//
