LOCUS       NZ_CP126795             9294 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_19 plasmid pEh19_3, complete
            sequence.
ACCESSION   NZ_CP126795
VERSION     NZ_CP126795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394408
            Assembly: GCF_030848045.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848045.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:08:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,760
            CDSs (total)                      :: 4,644
            Genes (coding)                    :: 4,562
            CDSs (with protein)               :: 4,562
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 25 of 82
            Pseudo Genes (incomplete)         :: 51 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 10 of 82
            Pseudo Genes (short protein)      :: 1 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126795.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_19"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh19_3"
                     /geo_loc_name="Switzerland"
                     /collection_date="2021-07-14"
     gene            join(7648..9294,1..318)
                     /locus_tag="QPR73_RS23680"
                     /old_locus_tag="QPR73_23645"
     CDS             join(7648..9294,1..318)
                     /locus_tag="QPR73_RS23680"
                     /old_locus_tag="QPR73_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            318..1049
                     /locus_tag="QPR73_RS23685"
                     /old_locus_tag="QPR73_23650"
     CDS             318..1049
                     /locus_tag="QPR73_RS23685"
                     /old_locus_tag="QPR73_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            1056..1586
                     /locus_tag="QPR73_RS23690"
                     /old_locus_tag="QPR73_23655"
     CDS             1056..1586
                     /locus_tag="QPR73_RS23690"
                     /old_locus_tag="QPR73_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1614..1793)
                     /locus_tag="QPR73_RS23695"
                     /old_locus_tag="QPR73_23660"
     CDS             complement(1614..1793)
                     /locus_tag="QPR73_RS23695"
                     /old_locus_tag="QPR73_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1862..2239
                     /locus_tag="QPR73_RS23700"
                     /old_locus_tag="QPR73_23665"
     CDS             1862..2239
                     /locus_tag="QPR73_RS23700"
                     /old_locus_tag="QPR73_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(2301..2600)
                     /locus_tag="QPR73_RS23705"
                     /old_locus_tag="QPR73_23670"
     CDS             complement(2301..2600)
                     /locus_tag="QPR73_RS23705"
                     /old_locus_tag="QPR73_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2590..2853)
                     /locus_tag="QPR73_RS23710"
                     /old_locus_tag="QPR73_23675"
     CDS             complement(2590..2853)
                     /locus_tag="QPR73_RS23710"
                     /old_locus_tag="QPR73_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(3159..3578)
                     /locus_tag="QPR73_RS23715"
                     /old_locus_tag="QPR73_23680"
     CDS             complement(3159..3578)
                     /locus_tag="QPR73_RS23715"
                     /old_locus_tag="QPR73_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3981..5666
                     /locus_tag="QPR73_RS23720"
                     /old_locus_tag="QPR73_23685"
     CDS             3981..5666
                     /locus_tag="QPR73_RS23720"
                     /old_locus_tag="QPR73_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5676..5933
                     /locus_tag="QPR73_RS23725"
                     /old_locus_tag="QPR73_23690"
     CDS             5676..5933
                     /locus_tag="QPR73_RS23725"
                     /old_locus_tag="QPR73_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            6018..6167
                     /locus_tag="QPR73_RS23730"
                     /old_locus_tag="QPR73_23695"
     CDS             6018..6167
                     /locus_tag="QPR73_RS23730"
                     /old_locus_tag="QPR73_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6271..6849)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          319..1046
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agggtggacg tggagcgccg ccgtgccagc cgggaggcgg ggaacgtgga gcatatcagc
       61 ggggacagct tcatccagaa ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacac
      121 ctgccggacg ggttcgcggt catgaccggg ctgggtgtgg cccggatggc gttcagcagt
      181 ccgctgcggg ttgagcgccg ggaaatcccg ttgcagcgtt tcccggtgct ggcgaaaacc
      241 gatcccctgg cggagttccg gcccccggcc gacggcggcg ggccgaaagc ggatgatgat
      301 tttgcgggga tttactgatg gcgctggagc tgtacaacgt cagccacgcg aaacgacagg
      361 cgcggaacgc ggagaaaacc cggctgaccc tgcgctggtt gcgggaagag ctgtgttcca
      421 cggcggagct ggttgcgcgg cggctgggga ttgcggcggt gcagccggtc taccgcttcc
      481 tcgacagcct ggtggcgaaa gggctgctgg tccggaccaa atatccggtg gacggccgtc
      541 aggtcagcgt gtgggggctg acgccgcatg gcgtggcgtt ctcgttcgac gaggacgaac
      601 cgctggcgga tgttattccg tttcagccgt caagggtgtc ggcagcacat ctgccgcacc
      661 ggctggccgt gcaatccctg cgccttgata tggaagccag aggggccacc ggctggcgca
      721 ttctgcaccg tctgagtctt aaaggcatga aagtgccgga cgccctggcg gagctggacg
      781 gtaaaacggt ggcgtttgag gtggagcgca ccgtcaagag ccgtcgccgg tatcaggaag
      841 tggtgtccgg gtatctgttc aaccgcaaag ctaacggcat cgatgaaatc tggtacatct
      901 gcccggaccg ggcgacgcag gtcagggtgc agcgggcaat cctgtcggtg gatgaaatcg
      961 tgaacccaca gaccggcgag gcccgcaaaa cggcagagct ggatcgtgaa cggttgtttg
     1021 cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg gtatttattg
     1081 aagacgcgga ggaccggaac ggcgagcgct gcatcatgcc gaccagcgag catctggaag
     1141 atatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc
     1201 agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac agcgacgaaa
     1261 aagccacgta ctttttcact ggtaaaaatc cgcaatggga acggaacaaa cccacaggaa
     1321 tgaagctgtg tgattacctc tatcgcaagg tggagcggtt cgccatcgag aacggtgtgc
     1381 aggtggttga tgccgggaag tgctcaccgg agcagattaa cgcgatgatt gacgtggtga
     1441 gtgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat
     1501 ataacagccg ctattttaac gtgccggtac ctgaactgcc ggaggacaaa cctgcccgcc
     1561 ggtggtggcc gttcggcaaa aaataaatgc cagcaccggc catgccggtg ctgttattcc
     1621 tccccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg tagccgctcg
     1681 cacatgtcgg ccggggtatc catatccagc tcattcagtt tatcgagcaa tagcagcgac
     1741 tgctccttaa taaatttagc catattgagg gctgtttttt cctgttttgt catgattctt
     1801 tccttccggg catggtgtgc gcctgtcagg tatactgtct caaaaatagc ggggtgagac
     1861 aatggcgtgg gtgacggtga gacaggcgac ggaactgacg ggtaaagccc gaagttccct
     1921 gtatcgggat atggcgaaag gccgcgtatc ataccgtacg gaagctgatg gcggccgggt
     1981 ggtggatacc agcgagctta tccgggtgta cggtgagtta agacaggatg agactcacga
     2041 gcgggacgag ttgagactgt cgggtgagac tgaaaaaatg gcctccgacg cgttgatcgc
     2101 ggagataaaa gcactcaggg aggaggtggc cgggctacgg caggaaatgc agtccatgag
     2161 actgctggaa cacaaaccgg agacagcaga gaccccggcc ggaacaccgc gccggtggtg
     2221 gccgtgggga aaacgctgac gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat
     2281 tgcgtccccg tcaccggtta ttagagaaca atatggggat tctggcgctg gccgaccacg
     2341 gccagaaccc ggacttttct ggtctggtca tcaacatcat agaggacatg ctgtcccatc
     2401 agactgatcc ggcgaacgtc ctcgccatag cgtgagtcca cttcatagag tcgcgggaag
     2461 tcggccagtt tgtccgccgc agcgagaacc cggtcggtaa cgttctgcgc atcccacagg
     2521 gatgaatttt ccgcgatata gtcaaacagc gtcgttacat cctcgatggc tccctgcttc
     2581 cattccacgt catacggcat cgcgcagttc ctgataacgg ccatgcaggc gctggcggag
     2641 ttgttccgtg gtcatacccg gacgcgtgtc agcacgggcg gcggtcactt tggcgcgaac
     2701 ctgatcttca tactctgcct gggccagttc ttcgttagcc agtcgctgca acagcgcagc
     2761 tttgtccggg ccaccttccg gcagtatgcg gggcaggctt tcagccagtt cccgaagaaa
     2821 gttggcgttt tcgtagtgtg tagtgttgct cattgcggag cctcccgggt taatgcggca
     2881 atatgaagga agtttagcag ggggcggtct gaaagaatat ggtcgtgtca gtcaggctga
     2941 tgaagcagaa agtcggtaaa aaagggctta cggcatccat tttacgtcaa aacatatgcc
     3001 atgcagttgc gtgcataggc atgcattagg ataaaattta tcgggcgcgt ttccggcagt
     3061 tttccggggg ggttgttgcc tgttttatcc cgtagccgcc ggaaacgccc tgagcccgtc
     3121 tgagcggtgc gcgtaatgac gcgttatggt aaatccactt actgtctgcc ttcatagcca
     3181 tcgagataaa cagctgaaat ggtgtcagcc agtagccttg attttttcgg tgtgaggtct
     3241 ttgctgcggt aatcggtacc cgcaaattgt gttgtgcctt ggagtgagtt taaaaattcc
     3301 tgactgtgaa actgctgaat gcgttgctgc acatcactcc gtgattttcc ggtcgcttta
     3361 tcctttgcgc ccatatccca gaactgccgg aaaaaaggca ccatcattcg tgttgtgtca
     3421 gctttctggg tggaaaaatt gggatcaaaa ccgtcatctg aagcatatac gtaatgccgg
     3481 gtatgctgtt ccacgctgcc ctttggagca caggcagtga gagttgctgt cgctgttatc
     3541 atggcgataa gacacagttt gctgccggaa cgtctcatga gcgtctcctt acctttaatt
     3601 cttttttctc attgagttat atatccgcag gctatcttat catcagtttc tgaccgtgtt
     3661 cagtgaccga acaacccgaa ccagaccccc cgtttctttt cctgtcccgg agggggcttt
     3721 ctggtatctc atataatgat ttcagtaaaa catactgagg ccggagaacg gcgccgtttg
     3781 tgctggtttt tgttgcatct ggaggggatt gcgcatggtg tgagtggctg tttttttgat
     3841 cattttcaca aaaacgcaaa tttacagact tgacatgaaa aacaaggcag cgtaattttt
     3901 gtactgtata taaaaccagt ggttatatgt acagtatttt attttagttt attgttttaa
     3961 aagtcaaaga ggaaaagtat atgagtggcg gagacggacg aggtccgggt aattcaggtc
     4021 tgggacacaa tggtggtcag gccagtggga atgtgaacgg tacatcaggt aaaggtggcc
     4081 cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg acgcatacac
     4141 ctaacgggga tattcataac tataacccgg gggagtttgg taacggcggg agtaaacccg
     4201 gtggtaatgg cggtaacagc ggcaatcata gcgggagctc tggtggcgga cagtcttcgg
     4261 ccaccgcgat ggccttcggt ctgcctgccc tggccactcc gggggctgaa ggactggctt
     4321 tatccgtttc cggcgatgcg ttgtcggccg ctgttgctga tgtgctggct gccctgaaag
     4381 gtccgtttaa gtttggtctg tgggggattg cgatttacgg agtgctgcct tctgagatag
     4441 caaaagatga tccgaaaatg atgtcaaaaa tcgtgacgtc attgccggca gacacggtaa
     4501 cggagacgcc ggtcagctcc ctgccgctgg accaggcgac ggtcagcgtc actaaacgtg
     4561 tggcggatat tgtgaaggac gagcggcagc atattgcggt tgtcaccggc cggccaatga
     4621 gtgttcctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc
     4681 cgggtctccc gtctctgcag gtgagcgtac ctaaaggtgt tccggccgcg aaagccccgc
     4741 caaaaggcat tattgctgaa aaaggtgatt cacgtccggc tggttttacg gccggtggta
     4801 actcccgtga ggccgttatt cgtttcccga aagagagcgg acagaaaccg gtctatgtgt
     4861 cggtgacgga tgttcttact ccggcacagg taaaacagcg tctggaggaa gaaaagcgtc
     4921 gccagcaggc atgggacgct gctcacccgg aagaggggct gaaaagagag tatgataaag
     4981 cgaaagctga gctggatgct gaagataaaa atattgcgac cttaaacagt cgcattgcat
     5041 cgacagagaa ggcgatcccc ggtgcaaggg ctgctgttca ggaagccgat aaaaaggtaa
     5101 aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat gaatatggct
     5161 ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag aatgagaaat
     5221 taaaagctgc ccagacatct ttaaacgaaa tgaatgaatc cttatccagg gataaggctg
     5281 cgctttccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa
     5341 ataaattaaa tgaggaaaag aaaaaacctc gtaagggaac taaagattac ggccatgatt
     5401 attttcctga tcccaagact gaagatatta aggggttggg agagttgaaa gagggtaaac
     5461 ctaaaacccc taaacaaggt ggtggtggta agcgggcccg atggtatggt gataaaaagc
     5521 gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtaccgc gccagtgatg
     5581 gcgaacacct cggggcattc gatccaaaaa cgggtaagca ggttaaaggg ccggatccaa
     5641 aacgaaacat taaaaaatat ctttaagagg taaatatggg gcttaaatta catattcatt
     5701 ggtttgataa gaaaaccgaa gagtttaaag gtggtgaata ctcaaaagac ttcggtgatg
     5761 atggttctgt cattgaaagt ctggggatgc ctttaaagga taatattaat aatggttggt
     5821 ttgatgttga aaaatcatgg gtttcgatat tacagccaca ctttaaaaat gtaatcgata
     5881 ttagtaaatt tgattacttt gtatcctttg tttaccggga tggtaactgg taagcaaaag
     5941 ttatcaagga tgatttgaaa tacagacttc cggtgttcag ggatgaatac cggggttttt
     6001 aaatacaggg aaggtatatg aaaaaggtaa aggttgtttt tctttttata ttgatttttt
     6061 caggctttct tctggtcgca tgtcaggcaa actatatccg ggatgttcag ggtggaacgg
     6121 tggcaccatc gtcctcctct gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg
     6181 atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac
     6241 ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac cgtggtaact
     6301 ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac tttggcgcac
     6361 acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc agttaccaat
     6421 ggctgctgcc agtggcgttt tgcgtgcttt tccgggttgg actcaagtga acagttaccg
     6481 gataaggcgc agcagtcggg ctgaacgggg ggttcttgct tacagcccag cttggagcga
     6541 acgacctaca ccgagccgag ataccagagt gtgagctatg agaaagcgcc acacttcccg
     6601 caagggagaa aggcggaaca ggtatccggg aaacggcagg gttggaacag gagagcgcaa
     6661 gagggagcga ttcatcggaa acggtgatga tctttaagtc ctgtcgggtt tcgccactcc
     6721 tgactgattc atggttgagc caccggctcc cacagatgca ccgaaaaagc gtctgtttat
     6781 gtgaactcag tcaggagggc ggagcctatg gaaaaacgcc acctgcgtgg ccttgccgtt
     6841 ttgcctcaca tgttagtccc ctgcttattc acggaatctg tgggtaactt tgcatgtgtc
     6901 cgcagcgccc gccgcagtct cacggccgga gcgtagcgac cgagtgagcg aggaggcgca
     6961 atttccccgg cacctacaag acggacaccg gacgcgctga tgtggcatat tgtcccctga
     7021 ttaagtcttt atattccctg ataataaatt accaggatag gtgaatcata tcggtttttc
     7081 gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttacgga gaaatacagg
     7141 tcatccccgc gagggcgtgg ctgacctcag ggggatatcc cctgcttacg ctggctggac
     7201 gccaccgacg cacggtctct ccccctcttt ctggctccgt ccggctggac gccggccacg
     7261 ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa atccacggtg
     7321 ctggacgcac ctggcgaagc agcaaagctg ctcctgattt tgcgggtggt cagccgctcg
     7381 ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt
     7441 ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg
     7501 ggcggaaaga ggctggcctc cgtggtgaaa gctgaacgca atcaccacgg acgtgaccgc
     7561 cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcac atcacgccgg
     7621 caaaccatcg tctgaaggag agagaacatg tttaatacgg attcgcttgc ctggaagtgg
     7681 cacattccgg agctggcccg gcagatttat ccgtgggtca gctggctgta ccaggagtat
     7741 tacggtctgg ccggggcttt cctagttatc ggcctgtttt tcggtctggc tgaactttgg
     7801 gccggacgga tgcgttttgt ggggctgagt cataaggagc gacgggagta tgccgatcgc
     7861 tataagcgcg ataataaacg gccagcgcca ttttcggtgc tggcctcgct ggattcgccc
     7921 gggcggatgg tcagggcacg tcgacatgtc tggaactacg cgtactggct gaccggcgcg
     7981 gccggtctgg cccttctggg ggggctggtt gtcgccgtgt ttatcgagag ctggggagaa
     8041 accgtcctgc gtccggacct ctacgcaaac gcagattatt acctgctgca gccggctaac
     8101 ctgttcggga ttgcctggtg cgcgggcggg ctggctgtcg ggattgtgtt ggcgcggctg
     8161 ctggcgttcc tgctgcttga cggtcatttt gcggctgctg acgaggcggt gaacgcgaaa
     8221 ctgaatcagg agagccggcg cagcagccag cggacaggag agatgaccga cgtgcgccac
     8281 ctgcatttcg gggagccggt accggttaat gcgctggccg atttttcctc ggagcaggcc
     8341 cggcaggagc aggcggtgtt tctgggtaag gatgagcagg gcaagccggt gctggttccg
     8401 cgtgatacct ggcgcaagac caacattcag atcctcggcc tgcccgggag cggcaagagc
     8461 gtgatggcca ccaacgcgct cattcgctgc gtacgcgatt tcggggacgc ggtggtgtac
     8521 ttcgacccca aaggggacgc atgggcgccg cacgtcttcc gggcgcactg tccggacttc
     8581 accctgctgg atctgcggcc ggggaaaccg gcgcagctga atctgttccg cgatctcgac
     8641 cagtacgcgc tgaaaaacct gctgattgcc gggcttaacc tgggcgagac cggcgacgtg
     8701 gcggaccact accgcatcag cgagcagaaa gcggcgaagc tgattgcgga gcagtttccg
     8761 cagggggcaa acattcagca ggtgctggcg gcggcgtacg cgctgccgga ggccctgaaa
     8821 aaggacgtga aggggttgat cactaggctg gaaaacgtcg ctgacctgag cgtcctgcag
     8881 acggacagtg gcatcgatgt ggcggggatt atcaacggcg gcggctgcct gtacgttatc
     8941 ggctcgatgg acgacgaggc ggtgatccgt gtgcaaaaaa tgctgtttgc ccgctgcgcg
     9001 caaatcatca ttgcgcggga cgagttccgg gagtggccac acgtcagcgt gatgctcgat
     9061 gaaattaaat atctgctgtc gaagtatgta ctgaacgcac tggggacgct gcgcagccgg
     9121 gactgcaatc tgttgctcgc gcaccagtcg ctgggcgatt tcgggcagtg cgggcaggat
     9181 ctgcccgctg aatttgtgaa aaccacggta ctggataaca cgccgatccg ctggttttac
     9241 cgggcggcca gcctggagtc cgcgcagtgg gcggcggggc agaccgggga aatc
//
