LOCUS       NZ_CP126791             4783 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_20 plasmid pEh20_3, complete
            sequence.
ACCESSION   NZ_CP126791
VERSION     NZ_CP126791.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394409
            Assembly: GCF_030848025.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030848025.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:59:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,161
            CDSs (total)                      :: 5,043
            Genes (coding)                    :: 4,940
            CDSs (with protein)               :: 4,940
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 29 of 103
            Pseudo Genes (incomplete)         :: 74 of 103
            Pseudo Genes (internal stop)      :: 18 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126791.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_20"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh20_3"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020-12-02"
     gene            complement(226..582)
                     /locus_tag="QPR81_RS25765"
                     /old_locus_tag="QPR81_25760"
     CDS             complement(226..582)
                     /locus_tag="QPR81_RS25765"
                     /old_locus_tag="QPR81_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(785..1102)
                     /locus_tag="QPR81_RS25770"
                     /old_locus_tag="QPR81_25765"
     CDS             complement(785..1102)
                     /locus_tag="QPR81_RS25770"
                     /old_locus_tag="QPR81_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1289..1531
                     /locus_tag="QPR81_RS25775"
                     /old_locus_tag="QPR81_25770"
     CDS             1289..1531
                     /locus_tag="QPR81_RS25775"
                     /old_locus_tag="QPR81_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(2202..2417)
                     /locus_tag="QPR81_RS25780"
                     /old_locus_tag="QPR81_25775"
     CDS             complement(2202..2417)
                     /locus_tag="QPR81_RS25780"
                     /old_locus_tag="QPR81_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2424..2915)
                     /locus_tag="QPR81_RS25785"
                     /old_locus_tag="QPR81_25780"
     CDS             complement(2424..2915)
                     /locus_tag="QPR81_RS25785"
                     /old_locus_tag="QPR81_25780"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142764349.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2950..3654)
                     /locus_tag="QPR81_RS25790"
                     /pseudo=""
     CDS             complement(<2950..3654)
                     /locus_tag="QPR81_RS25790"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3593..3916)
                     /locus_tag="QPR81_RS25795"
                     /old_locus_tag="QPR81_25790"
     CDS             complement(3593..3916)
                     /locus_tag="QPR81_RS25795"
                     /old_locus_tag="QPR81_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4567..>4689
                     /locus_tag="QPR81_RS25800"
                     /pseudo=""
     CDS             4567..>4689
                     /locus_tag="QPR81_RS25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(4032..4205)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          4129..4676
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2111..3603)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.8"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3600..3916)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 agccagttac caggttaagc agttccccaa ctgacttaac cttcgaaaaa ccgcctcccc
       61 aggcggtttt ttcgtttaca gagcaagaga ttacgcgtac agagcacgga tctcaagaag
      121 atcgtaagaa gaaagttaac atgagcgttt ttatccctca aaaagaaggc ttctagttat
      181 agaagccttc tttttgaggg ataacttata tttttttaaa aaaaatcatg caatagcatg
      241 tttcttctca gaactatcaa caatgtgttt cctttctatg atttcatgag cgggaatcac
      301 tctgtctatt tcttcttgca atcgagggct actagtaacc tcccataaat caacagactg
      361 ctgtatattt agccagaact ttgcagtggt atcgaaggct ttagccaacc taaaagccat
      421 atcggtagac agctttctat tattgttaac taaagcactg gcagagtttc tatgcacatt
      481 caacatttca gccaagtcat tgatttttaa accacttggt tcgagatatt cataaagaat
      541 aatatcccct acagttgatg gttttctagt tgcttgggac atgctttact cctaaatttt
      601 ttacttctct ctgttagatg aggaagataa gtatttcgtc tattaccagc tgtagcgatg
      661 agtaattatc ttaaatatag tcacgtcatt ttttttatta atcattattt gtaatgacat
      721 tttaatgtag gtgagggaac gcctcaccta cttgttttat aagataacag ccacgacctg
      781 ttatctatcg gtaatcatgt ggatcgagat acaaatcctc gactccgttt tcaccccact
      841 taaatatcaa gcggtacttc tcattcaccc gtatggatga ataccctgtc aatggagggt
      901 tgagattctc aaatcggttt cccgggggag accgcaaatc tccaacttta tcagcagcat
      961 tcagcatatc cagcttgcga cctaatgaag cactaatcgc agttggaatc tttcgattgg
     1021 ctttaccata tttgtaaaac tggtatagcc aatcgtctcg aaactgaatg cggcaatcat
     1081 catgttcatg atcttgctgc atttttcttt tacctcaatt tttgccgcaa cttggcaatt
     1141 ttttcgatca ccttcttacc tccgatatag tacagcaaac atatcaaatg cacaacagca
     1201 cagtgcactt gtgcaatcaa tggctatgct gtagaatgca ctcatgcaca gtgcaggtgt
     1261 gcaaaataaa tgataatttg gaggttgcat ggctcttgtt cgctcaggaa acaaaagcaa
     1321 gatttttgaa gcagatacga agtttaactc ggtttttgac actgaatata agccgggaga
     1381 aacatgtgaa tatgaaggga tttataaatg taccggttgc ggtaaagaag taactatcgc
     1441 aggcgggaaa acaatcccac cacaaaacca ccaccaacat acccccgcgc aagggaacat
     1501 tcgttggcaa ttgtttgttt atgctcagtg aggtatagaa aatggcagat cgtcgaccac
     1561 tcaacccaca cgttgtcgtt cctaaaccag gcggcggaac tcaacctcgt gaccgtaaca
     1621 atgacggaag ggtcagagga aaacgctcag atgcaggaaa gccacgaaaa taacagcaag
     1681 ccgccagcct ggcggctttt ttatatctgc agctgatact gatttaccat aacatccgtt
     1741 gggcgcaccg ctgaaacggg gttggctgcg tttccggtgc cagacgcgag aaagtggcga
     1801 cagacataac gaaaaaacac cagaaacgca gccgctgaat attacactgg tgcatagtca
     1861 tgcagccaga tgcatggatg ttaaagtcac actaaaatcc gcgccaatgc cttaaaaacc
     1921 gacaaatgct tctccagcca gtcccacagc tccgccgcca gatacaggca gttaagcaat
     1981 gattcaatga gatttaacag cttcatttcc cgctcctgaa taagtcgtga cctttttgat
     2041 cacgaaccta tttagcggga gaaataagag cccctcttcg tgcgggttgt cagctgcctg
     2101 gatggtttaa agtgtcggcc cgcggtaggt tttctgcggc tggcgctcca gctccagtgt
     2161 tttctgatac tcattctccc tctgccggag ctcgatggca ttcagttccc ggacaagcgg
     2221 cgcagctgct cgcttaaact cagcacctgc gtgctcaagt ccgacacgcg ctcgttcagc
     2281 accgcgttct cccgttgcat aagcccacac atgctccgcc attccgcgaa ggcgctctcc
     2341 cattcgtcca gcctttgcga gtagtcctgc tgtaactgct ctaatgcgct cagcaactgc
     2401 ttttccagct ctgtcatttt ccgctactcc tgtttcaggc ggatccatgt cgtcccgtcg
     2461 taggggatgg cttcggtttg catcccgggc ggcaggataa gaaacctccg gccgtcgctg
     2521 ctctcctgat agcgcactcc ccacgtccgg gcagtcattt tcgccagggt atcttcctgc
     2581 tgacgaagat gcgtgtaatt gtcggttatc tgctgcccct gccaccacag tatgctcccg
     2641 ctcgtgccga tcagcagcgc tgacaccatc aggatggtga accatgtccg cccggcagtc
     2701 cgcagtacgc tccgccggtt tttctccatc gcctcgctca tactctgctc gtgtgcgagg
     2761 atggcgtcgc tgattctcct cgcgcttgcg ctcagttccg ctctgacaga ctgctcgtgc
     2821 tcgctgaatg cggctttcag catctcgccg gtactctgct gctgcgcttt cgatttctgc
     2881 tctaagtcct tcgccagcgt taaaagactg ctcatagatg gctcccttca gtcggatgtt
     2941 tcgccccccg tccgggtcgg caatgctgat actgcttttc gtggtgcgca ccacctcaaa
     3001 acctgaccgt tccagcgcct cagtgacgtc ctgacggttt ttcagctccc cggacgaagc
     3061 aagggtcagc agtccgcgcg tgatggcttc agtggcctcc tgcttcgttt tcggcagtcc
     3121 ggacggcgtg accagcgccc ggcggttttc cggcgcgttc gggtcatgca gccccagtct
     3181 gccgttcacg atggtctgcc aggcatcgat gcgcggccgg tcggcgcggt cgtagtacgg
     3241 ctggagccgc ctgccggtca gcagttcggt attggggatc agaaagttca gctccagacg
     3301 ccccttgtcc gcgtgctcca cccacagaat gctgtactga tctttatcga gtccgggcat
     3361 cagaacccgc tcgaagctgg ccatcagttt ttcccgctgg ccgggaggca gatcggcctc
     3421 cgcaaacgac agtacgccgg aggtgtattt cttcacgtaa ggcgacgcgt caatcagctc
     3481 ccggacttct tccggcttcc cctgcaggac gctggcgcct tcacgctgcc ggtctttacc
     3541 cagcagatag tccaccggcc cggcgccacc gccgcgaccg cgtggatgaa atttaacgat
     3601 catcgtccgt tcctttctca agaacggcgt gccgcagccg ctcaagcccg gcatcgatgg
     3661 ccatcagcgc cgccacaatc tgtaccctgt catggccggt gccgccgccg gcgttaacac
     3721 ggcgggctat ctggttcagg ttattgccca taccggcaag ctggcgcagc agcgccggcg
     3781 agagtgaggg gagctttccg gtgcgcgcag gcttttcgtc caggcaggtc tgccgcatcc
     3841 acgccgccag cggcctgccg tcgcagcgct caagcagccg ccggtgctcg ccctccgtca
     3901 cccacatggt gagcatcttg ttccgtttct ctgacaaacc ggcctcctga taacgccggg
     3961 acgggcggga aagcctttcc cggtcggcgc ggggttggac aagccgcagg cgcgtcagtg
     4021 gcttttggcg ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat
     4081 actggcttaa tatgttggca cggatacgga aatcagtgaa gtgcttcatg tggcaggaga
     4141 aaaaaggcgg caacggcgcg tcagcagaat atgcgataca ggatatattc cgcttcctcg
     4201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtgcgggc ttacgaacgg
     4261 ggcggaaatt tcctggaaga tgccaggaag aaacttaaca gggaagcgat aaggccacgg
     4321 cagagccggt tttccatagg ctccgccccc ctgacgagca tcacagaatc tgacgctcaa
     4381 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctgatggct
     4441 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     4501 cgcgtttgtc tcattccacg cctgacactc ggttccgggt aggcagttcg ctccaagctg
     4561 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg cctgttccgg taactatcga
     4621 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccac tggtaacagg
     4681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4741 ggctacactg gaaggacagt atttggtatc tgcgctctgc tga
//
