LOCUS       NZ_CP126782             3750 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_18 plasmid pEh18_4, complete
            sequence.
ACCESSION   NZ_CP126782
VERSION     NZ_CP126782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394407
            Assembly: GCF_032202905.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_032202905.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:01:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,905
            CDSs (total)                      :: 4,790
            Genes (coding)                    :: 4,704
            CDSs (with protein)               :: 4,704
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 25 of 86
            Pseudo Genes (incomplete)         :: 55 of 86
            Pseudo Genes (internal stop)      :: 18 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            Pseudo Genes (short protein)      :: 1 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126782.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_18"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh18_4"
                     /geo_loc_name="Switzerland"
                     /collection_date="2021-12-20"
     gene            60..1079
                     /locus_tag="QPR62_RS24515"
                     /old_locus_tag="QPR62_24505"
     CDS             60..1079
                     /locus_tag="QPR62_RS24515"
                     /old_locus_tag="QPR62_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(1340..1738)
                     /locus_tag="QPR62_RS24520"
                     /old_locus_tag="QPR62_24510"
     CDS             complement(1340..1738)
                     /locus_tag="QPR62_RS24520"
                     /old_locus_tag="QPR62_24510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1915..2841
                     /locus_tag="QPR62_RS24525"
                     /old_locus_tag="QPR62_24515"
     CDS             1915..2841
                     /locus_tag="QPR62_RS24525"
                     /old_locus_tag="QPR62_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_014342223.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGYDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2844..3377
                     /locus_tag="QPR62_RS24530"
                     /old_locus_tag="QPR62_24520"
     CDS             2844..3377
                     /locus_tag="QPR62_RS24530"
                     /old_locus_tag="QPR62_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     Typing          61..835
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1916..2838
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agattgtcac gagaatacag gggcgaggtc ggtcgataac ctggaaggaa tcgtaacaca
       61 tgagcgccgc gcttcaatac ttcgaagaaa atttacccca ccgcccctat cacacggatg
      121 atctcgcttt cggccttcgc atctccggca aagggcgtgc gcttcttgcg cggtacatcc
      181 agcagaacca gcctcatgcg cagttctggc tggtttttga cgttgaccgc gagggggctg
      241 cgattgactg gagcgaccgg aacgcccccg cgccgaacat caccgttaaa aatcctgtga
      301 acggacacgc tcacctgctc tacgcactca acatcgccgt gagaaccgcg cctgatgcct
      361 cggttaaggc gctgaaatat gccgcagcgg ttgaacgttc gctgtgtgag aaactttgtg
      421 ctgatgtgaa ctacagcggc ctgatttgca aaaatccgtt ccacctggaa tggcaggtga
      481 tggagtggcg cgaggacgcc tataccctcg atgaactggc agattatctc gatttgagcg
      541 cctcagagcg tcgtagcatc gataaacatt acgggatggg gcgaaactgc cacctgttcg
      601 aaatgacgcg caaatgggct tacagggcga ttcgtcaggg ctggccagcg ttctcacagt
      661 ggcttgaagc ggtgatccag cgtgtcgaaa tgtacaatgc atcgcttccc gttccgcttt
      721 cactggctga atgtcgggct attggcaaga gcattgcgaa atacacgcac aggaacttca
      781 cgccggaaac tttcgcacag tatgtggctg atacgcacac gccagaaatt caggcagcac
      841 gcggtcgcaa gggcgggaag gccaattcgt cagaaaatca gtcggacaaa gggaaaaaaa
      901 gcgccgctgt tcggtggact gctaacgacg acaaacgtcg tagggcgctt gatatgtata
      961 tcttgggagc gtcaacggaa gatattgcag tggcggttgg tgttagcagc cggactattc
     1021 gacgctggat ggataattct ggagaatggc taaccaagaa acaaataatt aaatgttagt
     1081 taacaaagaa gtaacactac cccccgtttt ttgttaatgg ggggttaaat tcgcggacat
     1141 gaacaagcct atatcagata acagcgcctt tcggcgtctt tttgagcagc gggctttccg
     1201 gcggttttct cctctcagcc cagcaatggt gcggcttacc gcatgattga ggtggtcgtg
     1261 ctcgccgcag tctcatgacc gagcgtagcg agcgaatgag cgaggaagca ccagggaacc
     1321 ggaacacgga tggaggcact tacgcgtctg ggtttgggga tgtctccaga tcgtacttaa
     1381 tttccttaag tgcctgtaat atcctgtctt ttttatagca tagctcgcct gtttttgtgt
     1441 ctaccagtcc tgcttcgatc agtagcccag ccgcgagaaa gagtcggtga ttgcgttctc
     1501 gtcgttgttt ttttccctcg attcgttttt ggaccgcctg ttttcgttcg ctctcctgtg
     1561 cctgtaagtc gtttttttcc gctgttgcca gtacgtggag gagtttgcgt tcctgttctg
     1621 tgagtcctgc ttcgttgtcg agcaggtcaa taatctgtct ctgtcgtgcg gtctgttctc
     1681 cgagcttcct gatgaatccg atccgtttct tcagccattc gatgttgctg atgcgcatgt
     1741 tcgtggtgct ccctggtgtt tttttgcggt gtgttgtggt cgtgcagtat atcgcttgcg
     1801 gtcggcgggt gcagggggta tcttccgtgc taattcgtaa aattagaagg aagcgcactt
     1861 atatgtttct ctccttcgtc gagaaacagt gcgctcctgc ggagggctga aaccgtggca
     1921 acctatcacc tcagcgttaa atttggtggc aaggggcagg ctgcaaacca cgccgattac
     1981 atcgagcgca aagagaaata ccgcgaccgt caggatttgg aatattccgc acacggaaac
     2041 atgccggaat gggcgcgaga taatccctca catttctggc aggctgctga ccagtttgaa
     2101 cgtgcgaacg gttcgaccta tcgggaactt gaaattgcat taccccgtga actgacagcg
     2161 gaacagcgcc ttgaactggt gcaggatttt gtacgtcagg aagctggcga acggcacgcc
     2221 tggagttttg caattcacaa tccgaaagcg agcattgacg gcggcgaaca gcctcatgcg
     2281 catatcatga tgagccagcg tgtgaatgat ggcattgaac gtacaccgga acagtatttc
     2341 cggcgctata acgccaggta tcccgagcgg ggaggtgcga aaaaggattc cggaagcctg
     2401 actctcactc agcaaaaaga gcagttgcgg gaattgcgaa aacgctggga ggtgaagcac
     2461 aacgaacata tgagaaagca tggctttgaa aggggcttca ttgactgccg gacgctgaaa
     2521 gaacagggca ttgaacgacg tcccgaagtg catctgggtt ttgaagctgc cggacggctt
     2581 gatgatgccc agcgtcatga catcatgcaa aaacggtctg aaccggacta ttcccgccat
     2641 cttgaacagg cgcgaacggt cgggtatgac atcagggaat ggctggacac tccggttaag
     2701 caacatgaac tggtcagaag gctgcaacat gatgttgcgc gggagcatgg ctttgacatg
     2761 agagacagag tgagcagaca gaaccgaact gaacctgaac gggttaaatc accgtcacgt
     2821 ggattttcta tgggattctg aacatggcca gcacagaaga aaaattactc acgctcctgt
     2881 tatcggtgga gcatctgcaa cataccgcaa tggagcaaca gaaagctgtt gctgattctc
     2941 tggctgtggc aagggaaaca cttcgcggat atcgggatat ttctgacggt gtggcaaaac
     3001 aggtcaggga tgaagtcaga aacgaggtgc agaagctgga tgttgcaggg ctgataggtg
     3061 agcgcatcag ccaggatttt cagcggctgg aagagtcagt cgcaaacatg aaggcaaacg
     3121 cggatgccct gaacagggat attgcgacaa ttcacagcaa tatcgtgcag gaatacaaca
     3181 cccttaaagg gtatagctgg aaatggctgg gtggggtgtc agtggctttt ctggttatgt
     3241 tctgtgtgct tggatgggga atgaaaacaa tgctcgacag gaactacaac gccacagcgg
     3301 cggtgtatca gaaagttgtg acactggaag ataaactggc ggagaaacct gtcacgggca
     3361 agagccggaa aaactgacgc gcttcgcttg tccaacgcct cacgacccgc gaaaaaattc
     3421 cgcgtcccgt tcaccgtttt ctgatgagaa agtttgctgt tttgacccgt tcgcccttct
     3481 gaacgctcga aacagatcgc gcatcgcttt cgtgcatagt tatgcaacgc gctaaaaacg
     3541 attctgacgc atttttctgg tttagcctgg tgtttccttg tctttttgcg ttttttgcgc
     3601 cagagagcga ctgagggcgt tttaaggggg cgtacaacgg gagttatggt aaatggatcg
     3661 gttttgcggg aaggctcgac aggttttgtt gttgggggta gtgtaagcga ctgaaaaaca
     3721 aacgcccctg aaattcgtgt ctctccgcca
//
