LOCUS       NZ_CP126772             3372 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_23 plasmid pEh23_5, complete
            sequence.
ACCESSION   NZ_CP126772
VERSION     NZ_CP126772.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394412
            Assembly: GCF_030847965.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030847965.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:59:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,918
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 33 of 108
            Pseudo Genes (incomplete)         :: 78 of 108
            Pseudo Genes (internal stop)      :: 20 of 108
            Pseudo Genes (multiple problems)  :: 19 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126772.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_23"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh23_5"
                     /geo_loc_name="Switzerland"
                     /collection_date="2017-08-29"
     gene            639..1199
                     /locus_tag="QPR74_RS24560"
                     /old_locus_tag="QPR74_24520"
     CDS             639..1199
                     /locus_tag="QPR74_RS24560"
                     /old_locus_tag="QPR74_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1206..1439
                     /locus_tag="QPR74_RS24565"
                     /old_locus_tag="QPR74_24525"
     CDS             1206..1439
                     /locus_tag="QPR74_RS24565"
                     /old_locus_tag="QPR74_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2755..2946)
                     /locus_tag="QPR74_RS24570"
                     /old_locus_tag="QPR74_24530"
     CDS             complement(2755..2946)
                     /locus_tag="QPR74_RS24570"
                     /old_locus_tag="QPR74_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2995..3333
                     /locus_tag="QPR74_RS24575"
                     /old_locus_tag="QPR74_24535"
     CDS             2995..3333
                     /locus_tag="QPR74_RS24575"
                     /old_locus_tag="QPR74_24535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_266097495.1"
                     /translation="MKREKMLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTG
                     KLPTLAPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVR
                     EQGVRDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2591..2674
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="95.0"
                     /evalue="4.11e-16"
     Typing          3..1522
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.383"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2978..3330
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.593"
                     /coverage="100.0"
                     /evalue="4.48e-59"
ORIGIN
        1 cgattacctg ctggggcggg agcgtaaccg cgaaggcgca acggtgctcc ggggtaaccc
       61 ggaagaagtg cgggaactca ttgattccac gccattttcg aagaaataca cgtcaggcgt
      121 tctgtcgttc gcggagaagg agctaccacc gggtgaacgc gaaagagtga tgacgagctt
      181 tgagcgtgtt cttatgcccg gtctcgaaaa agaccagtac agcattctct gggtggaaca
      241 ccaggataag gggcggctcg aactgaattt tgtcatcccg aacatggagc tacagagcgg
      301 taagcgcctc cagccgtact acgaccgtgc ggacaggccg aggataaatg cctggcagac
      361 gctggttaat catcattacg ggctgcgtga cccgaacgca ccggagaaca gacggacgct
      421 tgtcacgcca aataatctgc caaaagcgaa acaggaggcc gcagaagcga ttacacgggg
      481 tcttgaggct ctttaccatg ccggagcaat aaaaacgcgt caggatgtca cagaggcgct
      541 tacagcggca ggttttgagg tggtgaggac aaccaaaagc agcatcagta ttgccgaccc
      601 ggaggggggg cggaatctca ggctaaaggg ggcgctgtat gagcaatctt ttacaaatgg
      661 gaacagactt cgagaagaaa ctgaaagagc gagccgcttc taccgagaac atgctgaaca
      721 gcgagttcag gaagctcgaa gagtctgtag acaaggcatt gagcttaaac aggcagaaaa
      781 tcagagacgc tatcagcgaa catacaacat ccgtgaaaaa gcaactggat acgctgagca
      841 ccaccgtgag catgcagttc agcaccacag aggccgaact gtcccggcag cagaaaaaac
      901 tgctctggcg ggtgatcaaa gggcggatac tgtttccggc gctgaccgcg ctcagcgtca
      961 cgggcgggat tttcctgggg tgctgggggc tgatggagtg gcaggaaagc aagattgcga
     1021 aaaatatcct gactatcaga gagcaggaaa acacgctggc gaaactcgag gcaaaaacct
     1081 ggggggtgac gttcgtcaat ggcgagaacg ggaaatttct ggtgttgccg gacggggtga
     1141 aaggggaaaa cacctggacg gtgggcgaca agaacgccgt gaggctggtc agggagtaaa
     1201 cgagtatgac ggaactggaa gggcatttgc tgaacgcctt agagcatttg caacaggact
     1261 atatgcggcg gctgaacgag tgggagagcg ccttcgcgga attgcagaag atgcacgagg
     1321 ttacgcaacg gaacaacgcg atactgaacg agcgcgtcgt gatattgagt cagcaggtgc
     1381 agagattagc cgggcaagtg gatcgcttga gtcggttatt tatcgcgaac aacagctaag
     1441 ggatgaacag gagcgtgccc ggtacagaca tgaggtacag gaacgcgcgg cacgggttcg
     1501 gagtgggcca tcaatggaga tgtgaccggg gaagtgacga aatcttgcca ttttgacccg
     1561 ttcgacggct ggggcgagcg aaacaaatcg tgtgccgtat ttgtgcatag ctatgcaaca
     1621 tcctgaaaac gcatttcacg cgtttttggg gattcagcca ggggaaaaca tggttttttt
     1681 actttttgag cgtaaagacg tgaaaaatct cgttattgca cttgcgatct ttcgtcggcg
     1741 ggcgtagaac gaaaacagct cgccaggaag gcgagctgtt tcgggttcat tgaggtctct
     1801 gacctcctca atgttcctgt ggaaactgtt gagatccaca acagaaacca gttcattcag
     1861 tttgcatgag ccttctgtat tcttcaagaa tttttcaaca gcacagtctc ccaggaaccc
     1921 cgaaaaaaac ctgtttttgc agatacttgc gtatttgcga gaatcgtttg cggttggggg
     1981 agctgtgacg acggcgaccg cacgcgtgag agttccgcta ccggcgcaga gagtttcatc
     2041 gacagcggac acctgatgag ttgccacaaa aaccgtatgt agcgcggcta gattggttga
     2101 gttagacctt ggcggcagtt cccggtatgt ggtggcgtga cgggtgagat ggtgcggcga
     2161 agacgcatac gtcacgcagg cgcgggttct tccactgacg cgccccgtga tcgatggcct
     2221 tggctccgtc cggcatatcg agagcggcaa actacccgtc ccttcactgg gaggggtgag
     2281 ttgttgccgt tccctccgac cttccaccat ccagcatatc gcacgatccc atgtgcaccg
     2341 ctactcctga taactccagt gcaaaaaaaa aacggatctg catcacaacg agagtgatcc
     2401 caccgctcgc cgcagcccca gcgaccgaac gtagtgagcg agtgggcgag gaagcggaag
     2461 agtcacagag gcaagcattg tgcacttacg cgcctgtgcc agtgtccgcg tgtgtgttgt
     2521 ttatcgggcg cgctgttttt acgcatctgt gcggcttttt actcctgaca tggtgcactt
     2581 cccatccact ctgcaccgat gcaaaatgag gaaagccagt atacacatcg ctatcgctct
     2641 gtgaactggg tcagggctgc gccccgacac ccgcactgac gcgccctggc gggcttgtct
     2701 gctcccggca tccgctcaca ggcaagctgt gaacgtctcc gggagctgca tgtttcagtt
     2761 gctttcaccg tcatcgccga agcgtgcgag gcaactgcgg tagagttcgt cggcgtggtc
     2821 gtgaagtgat tcgcagatgt ctgcctgttc gtccgcgtcc agatcgttga gtttttccag
     2881 cagggttagc gtctggcttc tgataaagcg agccatgtta agggctgttt tttcctgttt
     2941 ggtcatggtc ttaaggctcc ttgtatgggg gatttgttag ggggaatgat accgatgaaa
     3001 cgcgagaaga tgctcacgat acgggttacc gatgaggagc acgcccggtt actggaacgc
     3061 tgcgagggta aacgactggc ggaatggatg cggcgggtct gtctggggga gccggttgca
     3121 cgcacaggaa aactgccgac actggcaccg ccattgctgc ggcatctggc tgccatcggg
     3181 aacaacctga accagacagc ccggaaggtg aacagcggcc agtggtcttc gattgaccgg
     3241 gttcatgtgg tggccgcact gatggccatc gagggtgaat tgcgccagtt gcggcaggct
     3301 gtcagggaac agggggtacg ggatgatagt taaatttcat gccaggggga aaggcggtgg
     3361 aagtggcccg gt
//
