LOCUS       NZ_CP126771             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_23 plasmid pEh23_4, complete
            sequence.
ACCESSION   NZ_CP126771
VERSION     NZ_CP126771.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394412
            Assembly: GCF_030847965.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030847965.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:59:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,918
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 33 of 108
            Pseudo Genes (incomplete)         :: 78 of 108
            Pseudo Genes (internal stop)      :: 20 of 108
            Pseudo Genes (multiple problems)  :: 19 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126771.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_23"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh23_4"
                     /geo_loc_name="Switzerland"
                     /collection_date="2017-08-29"
     gene            complement(<174..878)
                     /locus_tag="QPR74_RS24530"
                     /pseudo=""
     CDS             complement(<174..878)
                     /locus_tag="QPR74_RS24530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(817..1140)
                     /locus_tag="QPR74_RS24535"
                     /old_locus_tag="QPR74_24495"
     CDS             complement(817..1140)
                     /locus_tag="QPR74_RS24535"
                     /old_locus_tag="QPR74_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1784..1894
                     /locus_tag="QPR74_RS24540"
                     /old_locus_tag="QPR74_24500"
     CDS             1784..1894
                     /locus_tag="QPR74_RS24540"
                     /old_locus_tag="QPR74_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(2149..2610)
                     /locus_tag="QPR74_RS24545"
                     /old_locus_tag="QPR74_24505"
     CDS             complement(2149..2610)
                     /locus_tag="QPR74_RS24545"
                     /old_locus_tag="QPR74_24505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3010..3924)
                     /gene="lpxO"
                     /locus_tag="QPR74_RS24550"
                     /old_locus_tag="QPR74_24510"
     CDS             complement(3010..3924)
                     /gene="lpxO"
                     /locus_tag="QPR74_RS24550"
                     /old_locus_tag="QPR74_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(4195..4410)
                     /locus_tag="QPR74_RS24555"
                     /old_locus_tag="QPR74_24515"
     CDS             complement(4195..4410)
                     /locus_tag="QPR74_RS24555"
                     /old_locus_tag="QPR74_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1242..1428)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1408..1894
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(4..827)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.455"
                     /coverage="99.0"
                     /evalue="1.39e-179"
     Typing          complement(4098..4759)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.753"
                     /coverage="99.0"
                     /evalue="7.4600000000000035e-109"
ORIGIN
        1 tcttctcgct ttcgctcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt
       61 tcagcatctc gccggtactc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca
      121 gcgttaaaag actgttcata gatggctccc ttcagtcgga tgtttcgccc cccgtccggg
      181 tcggcaatgc tgatgctgct ttttgtggtg cgtaccacct caaaacctgc gctttccagc
      241 gcctcagtga cgtcctggcg ggttttcagc tccccggacg aggccaaggc cagtaaaccc
      301 cgtgtaattg cctgagcggc ttcctgcttc gtttccggca gcgcggacgg cgtgaccagc
      361 gcccgccggt tttcaggcgc gttcgggtca tgcagcccca gcctgccgtt cacgatggtc
      421 tgccaggcat caatgcgcgg acggtcggcg cggtcgtaat acggctggag acgcttgcca
      481 gtcagcagct ccgtgttcgg gatcaggaag ttcagctcca gccgcccctt atcctggtgc
      541 tcaacccaca gcacgctgta ctggtctttg tcgagtccgg gcatcagaac ccgttcaaag
      601 ctcgccatca gcttttcgcg ctgcccgggc ggtaaatcct tttcggcaaa ggacaggacg
      661 ccggatgtgt acttttttgc gtagggtgag gcgtcgataa gctccctgac ttcttccggc
      721 ttcccctgca gaacggtggc gccctcgcgc tgtcggtctt tccccagcag ataatccacc
      781 ggaccggcgc caccgccgcg ccctcgcggg tgaaacttaa cgatcatcgt ctgcaccctt
      841 ttccagcacg gcatgccgca gccgctcgag tccggcatct atggccatca gcgcggcgac
      901 gacctgcacg cggtcgtgcc cgctgccacc accggcgtta acctgccggg caatctggtt
      961 aaggttgttc cccatgccgg caagctgacg aagcagcgcc ggcgagatcg agggaagctt
     1021 gccggcgcgg gcgggtttct cgtccaggca cgtctggcgc atccaggctg caagctgttt
     1081 accgtcgcag cgctccagca gacgccggtg ctcatcctcg gtcacccaca tcgtgagcat
     1141 tttgctgcgt ttgtctgcca atccggcctc ctgataacgc cgggacgggc gggaaatctt
     1201 ttcccggtcg gcgcggtgtt ggacaagccg caggcgcgtc agtggtttta gcgggtttcg
     1261 gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct taatatggta
     1321 aatcgaagtg ggaattcagg gaagtgcttc atgtggcagg taaaaaatgc gtctgtgggg
     1381 cgtaagtgcg aaatgctggg gacgatgaaa tgacgcctcc tcgctcactc ggtcgctacg
     1441 ctcctgccgt gagactgcgg cgggcgttac cggctcacga atggcgggat aaagagatgg
     1501 cgctcaaatc aggaagggct gggaaaagga tgcggcgttg ccgtttttcc ataggctccg
     1561 cccccctgac aagcatcacg aaatatgacg ctcaaatcag tggcggcgaa acccgacagg
     1621 actataaaga tcccaggcgt ttccccctgg tagctccctc gtgcgctctc ctgttcctgc
     1681 ctttcggttt accggtgtca ttccgctgtt atgtggccgc gtttgtctca ttccacgcct
     1741 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg acccccccgt
     1801 tcagtccgac taccacgccc attccggtaa ctatcaactt gagtccaacc cggaaagaca
     1861 cgacaaaatc gccattggcg gtagccactg gtaagcagga tgtgcgagag atgtatcgag
     1921 agttcttgaa gtggtggccg gactacggct acactggaag gacagatttg gtgacacgtc
     1981 tcgcacaaga ctgttaccac ggttcagaag ttccccaact tactgaacct tcgaaaaacc
     2041 acctccccag gtggtttttt cgttttcaga gcaagagatt acgcgcagag caaaacgatc
     2101 tcaagaagat cattttacaa attggataat atccctccgc ccctaacact aatataaaat
     2161 tatcgggctt gcatgcccct caccattttc cccatatcca ccaccaaaca atagttcgtc
     2221 gagttctttg ctagcttgat caatacacgc agccacatga atagcagaag ggtactcatc
     2281 atactgaaaa ttatcgcttt tttcaggagg gagtcgatca taatacggat gacaatccac
     2341 ataataatga cgtattagca tcccagtttt atcttcgtac tctgatggct gcaacgtact
     2401 ccctgggaaa gcttttgcaa catgtatagc agcttgaaag gtatcaaaat atcgcccaaa
     2461 cattggcgtt tcaaagctag gcgactctgt cgaaaaatcc cccataactt gcgccaaata
     2521 accattatct atctgccatt gaacatctga tcccatagcc cactctccca gagttactcc
     2581 taataaataa ccataatatg tatttaccat aacgcttctt ggccgcaccc cctgagagcc
     2641 cctcagcgcg tttctgcggc ctgagggccg tttccggcac ctttccccgg cccaaaccct
     2701 gaaaaacgcc cctgataatt tctgcgcggc tgcatggcta tgcacaagag tgaatggcgg
     2761 ccggtttcgt gaaggaaata ccgtgaaagc tccgaaagaa acgcttaatt gcacagcagc
     2821 acatgaacgt gatcagatga aatctgtcta ctgccgcact tcttcacgaa aggcccgctg
     2881 agcgtcagat tcgggccttt cttactaaaa agatttcaat agaatttttt agcaggcgat
     2941 ttttatctat aaccatacat tgagcataga agacccaaat ttatgtatga ctcaatagaa
     3001 aaaaacaaat cacagccatc taaacactga gacagcaagc caaacaaaaa tcaaagaaat
     3061 aactccattt ttaatcaaat aatatagttt tcgattcctt ttttttagct tctgacctgc
     3121 atcacgaata gcattaacat atttaaataa atgatttata gcaccagtcc tgtcattatt
     3181 atcgttgggt gatgctgccg ctgacattag caccccacct atccacctat ttattttttg
     3241 tgaccaccgc cacttcatcg gcctttctat atcacagaac aaaataatac gtgtctgctc
     3301 ggtatcattc tgagcccagt gaacaaaagt ttcgtcaaaa attatcactt caccatcatg
     3361 ccaactgcgg cgtacaccat caacttcaat aaaacaccgt tcatcatttg gtgttgataa
     3421 tcctaaatga taacgaactg aaccggcata agggtcacga tgctttccta aatggcttcc
     3481 tgaaggcaat tcagcaaaca tcgctgcttt aatacaggga atagagctaa ccagttgggt
     3541 tgttactggg caaagccgct ccgctgatgg atgagcatct ccataccact tgaggtaaaa
     3601 gcgcttccaa ccgcgcttaa aaaatgtatt aaatcctgca tcattatttt tttcagatga
     3661 tttaatgtgc ccttgcaaat taacagcttc gtctcttata acctgccagt tatcaattaa
     3721 tggttgaagt tcaggaaatg tttctggggg aaaccatgcc tgctttgacg gcagcttcga
     3781 aaagcctgtc ataaacatat ttatcggagc cataaatgtt gaatgatcaa ataactgcct
     3841 ggaaaacttt tgttttattt taccgcgaga atgcgcatac aatacactga taagaaaaac
     3901 aaaaataata ataagtgaca gcatgtgtat attccttgtt cacgccaata ataaaaatct
     3961 cttaaagata acataatgaa tgcacttaaa ttataatcta aatgctattt atcaatagta
     4021 taatcgaagc ttcaccagaa catatctact acacaggcag agaggatttc agcaggaatt
     4081 atgctgaaat gaatcaaagg gacggaccgt gatatacccg ctgtcgctgt gatcgttcga
     4141 gctccagcgc tttctgatgc cgcgcctccc gcacctgctg cagctcgacg gcattcagcc
     4201 gctcgacaac cggcgcagct gatcgctcaa actctgcacc tgcctgctca agcccgtgac
     4261 gcgctcgctc agcgccgcgt tctcccgttg cataagacca gacatcgtcc gccattccgc
     4321 gaaggcgctc tcccactcgt ccagcctttt cgagtagtcc tgctgtagct gctctaatgc
     4381 gctcagcaac tgtttttcca gctctgtcat gtgctatttc cccgccagta tcatccagct
     4441 cgaatcctct ccgaaccgtc ccgcttccgg gttcaccctc acgcagcggc gtctctgctc
     4501 gccgcaggtc gagagctgca cgccgctgtt ccgctcggac agcatcgcct gcgtgctctt
     4561 ctgctcccgg atgctcacgt aattgtcgag tatctgctga ctctgccacc acagaatggc
     4621 cgcgttcgag gcgatcagca gcacggagac caggacgatg gtcagccacg tctggctgac
     4681 catgcgcagc atccctttcg tacgctggct catggctgag gacagcttcc ggtcgtggtc
     4741 gaggatggcg gcgctgattc
//
