LOCUS       NZ_CP126765             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain Ehh_24 plasmid pEh24_4, complete
            sequence.
ACCESSION   NZ_CP126765
VERSION     NZ_CP126765.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35394413
            Assembly: GCF_030847945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss
            companion animal clinic and their genetic relationship to clinical
            human isolates
  JOURNAL   J Antimicrob Chemother 78 (12), 2950-2960 (2023)
   PUBMED   37923369
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Dona,V., Nordmann,P., Kittl,S., Schuller,S., Bouvier,M., Poirel,L.,
            Endimiani,A. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2023) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030847945.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:59:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,940
            CDSs (total)                      :: 4,823
            Genes (coding)                    :: 4,713
            CDSs (with protein)               :: 4,713
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 31 of 110
            Pseudo Genes (incomplete)         :: 81 of 110
            Pseudo Genes (internal stop)      :: 19 of 110
            Pseudo Genes (multiple problems)  :: 18 of 110
            Pseudo Genes (short protein)      :: 1 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126765.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Ehh_24"
                     /isolation_source="lower respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pEh24_4"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018-08-09"
     gene            complement(200..310)
                     /locus_tag="QPR70_RS24655"
                     /old_locus_tag="QPR70_24620"
     CDS             complement(200..310)
                     /locus_tag="QPR70_RS24655"
                     /old_locus_tag="QPR70_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            954..1277
                     /locus_tag="QPR70_RS24660"
                     /old_locus_tag="QPR70_24625"
     CDS             954..1277
                     /locus_tag="QPR70_RS24660"
                     /old_locus_tag="QPR70_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1486..>1920
                     /locus_tag="QPR70_RS24665"
                     /old_locus_tag="QPR70_24630"
                     /pseudo=""
     CDS             1486..>1920
                     /locus_tag="QPR70_RS24665"
                     /old_locus_tag="QPR70_24630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1955..2440
                     /locus_tag="QPR70_RS24670"
                     /old_locus_tag="QPR70_24635"
     CDS             1955..2440
                     /locus_tag="QPR70_RS24670"
                     /old_locus_tag="QPR70_24635"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2444..2659
                     /locus_tag="QPR70_RS24675"
                     /old_locus_tag="QPR70_24640"
     CDS             2444..2659
                     /locus_tag="QPR70_RS24675"
                     /old_locus_tag="QPR70_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2930..3844
                     /gene="lpxO"
                     /locus_tag="QPR70_RS24680"
                     /old_locus_tag="QPR70_24645"
     CDS             2930..3844
                     /gene="lpxO"
                     /locus_tag="QPR70_RS24680"
                     /old_locus_tag="QPR70_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            4244..4705
                     /locus_tag="QPR70_RS24685"
                     /old_locus_tag="QPR70_24650"
     CDS             4244..4705
                     /locus_tag="QPR70_RS24685"
                     /old_locus_tag="QPR70_24650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          667..850
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(201..684)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          1268..2757
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgctctgcg cgtaatctct tgctctgaaa acgaaaaaac cacctgggga ggtggttttt
       61 cgaaggttca gtaagttggg gaacttctga accgtggtaa cagtcttgtg cgagacgtgt
      121 caccaaatct gtccttccag tgtagccgta gtccggccac cacttcaaga actctcgata
      181 catctctcgc acatcctgct taccagtggc taccgccaat ggcgattttg tcgtgtcttt
      241 ccgggttgga ctcaagttga tagttaccgg aatgggcgtg gtagtcggac tgaacggggg
      301 ggtcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      421 caggagagcg cacgagggag ctaccagggg gaaacgcctg ggatctttat agtcctgtcg
      481 ggtttcgccg ccactgattt gagcgtcata tttcgtgatg cttgtcaggg gggcggagcc
      541 tatggaaaaa cggcaacgcc gcatcctttt cccagccctt cctgatttga gcgccatctc
      601 tttatcccgc cattcgtgag ccggtaacgc ccgccgcagt ctcacggcag gagcgtagcg
      661 accgagtgag cgaggaggcg tcatttcatc gtccccagca tttcgcactt acgccccaca
      721 gacgcatttt ttacctgcca catgaagcac ttccctgaat tcccacttcg atttaccata
      781 ttaagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg ccccgaaacc
      841 cgctaaaacc actgacgcgc ctgcggcttg tccaacaccg cgccgaccgg gaaaagattt
      901 cccgcccgtc ccggcgttat caggaggccg gattggcaga caaacgcagc aaaatgctca
      961 cgatgtgggt gaccgaggat gagcaccggc gtctgctgga gcgctgcgac ggtaaacagc
     1021 ttgcagcctg gatgcgccag acgtgcctgg acgagaaacc cgcccgcgcc ggcaagcttc
     1081 cctcgatctc gccggcgctg cttcgtcagc ttgccggcat ggggaacaac cttaaccaga
     1141 ttgcccggca ggttaacgcc ggtggtggca gcgggcacga ccgcgtgcag gtcgtcgccg
     1201 cgctgatggc catagatgcc ggactcgagc ggctgcggca tgccgtgctg gaaaagggtg
     1261 cagacgatga tcgttaagtt tcacccgcga gggcgcggcg gtggcgccgg tccggtggat
     1321 tatctgctgg ggaaagaccg acagcgcgag ggcgccaccg ttctgcaggg gaagccggaa
     1381 gaagtcaggg agcttatcga cgcctcaccc tacgcaaaaa agtacacatc cggcgtcctg
     1441 tcctttgccg aaaaggattt accgcccggg cagcgcgaaa agctgatggc gagctttgaa
     1501 cgggttctga tgcccggact cgacaaagac cagtacagcg tgctgtgggt tgagcaccag
     1561 gataaggggc ggctggagct gaacttcctg atcccgaaca cggagctgct gactggcaag
     1621 cgtctccagc cgtattacga ccgcgccgac cgtccgcgca ttgatgcctg gcagaccatc
     1681 gtgaacggca ggctggggct gcatgacccg aacgcgcctg aaaaccggcg ggcgctggtc
     1741 acgccgtccg cgctgccgga aacgaagcag gaagccgctc aggcaattac acggggttta
     1801 ctggccttgg cctcgtccgg ggagctgaaa acccgccagg acgtcactga ggcgctggaa
     1861 agcgcaggtt ttgaggtggt acgcaccaca aaaagcagca tcagcattgc cgacccggac
     1921 ggggggcgaa acatccgact gaagggagcc atctatgaac agtcttttaa cgctggcgaa
     1981 ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc
     2041 attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcgaga agagaatcag
     2101 cgccgccatc ctcgaccacg accggaagct gtcctcagcc atgagccagc gtacgaaagg
     2161 gatgctgcgc atggtcagcc agacgtggct gaccatcgtc ctggtctccg tgctgctgat
     2221 cgcctcgaac gcggccattc tgtggtggca gagtcagcag atactcgaca attacgtgag
     2281 catccgggag cagaagagca cgcaggcgat gctgtccgag cggaacagcg gcgtgcagct
     2341 ctcgacctgc ggcgagcaga gacgccgctg cgtgagggtg aacccggaag cgggacggtt
     2401 cggagaggat tcgagctgga tgatactggc ggggaaatag cacatgacag agctggaaaa
     2461 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
     2521 ggagagcgcc ttcgcggaat ggcggacgat gtctggtctt atgcaacggg agaacgcggc
     2581 gctgagcgag cgcgtcacgg gcttgagcag gcaggtgcag agtttgagcg atcagctgcg
     2641 ccggttgtcg agcggctgaa tgccgtcgag ctgcagcagg tgcgggaggc gcggcatcag
     2701 aaagcgctgg agctcgaacg atcacagcga cagcgggtat atcacggtcc gtccctttga
     2761 ttcatttcag cataattcct gctgaaatcc tctctgcctg tgtagtagat atgttctggt
     2821 gaagcttcga ttatactatt gataaatagc atttagatta taatttaagt gcattcatta
     2881 tgttatcttt aagagatttt tattattggc gtgaacaagg aatatacaca tgctgtcact
     2941 tattattatt tttgtttttc ttatcagtgt attgtatgcg cattctcgcg gtaaaataaa
     3001 acaaaagttt tccaggcagt tatttgatca ttcaacattt atggctccga taaatatgtt
     3061 tatgacaggc ttttcgaagc tgccgtcaaa gcaggcatgg tttcccccag aaacatttcc
     3121 tgaacttcaa ccattaattg ataactggca ggttataaga gacgaagctg ttaatttgca
     3181 agggcacatt aaatcatctg aaaaaaataa tgatgcagga tttaatacat tttttaagcg
     3241 cggttggaag cgcttttacc tcaagtggta tggagatgct catccatcag cggagcggct
     3301 ttgcccagta acaacccaac tggttagctc tattccctgt attaaagcag cgatgtttgc
     3361 tgaattgcct tcaggaagcc atttaggaaa gcatcgtgac ccttatgccg gttcagttcg
     3421 ttatcattta ggattatcaa caccaaatga tgaacggtgt tttattgaag ttgatggtgt
     3481 acgccgcagt tggcatgatg gtgaagtgat aatttttgac gaaacttttg ttcactgggc
     3541 tcagaatgat accgagcaga cacgtattat tttgttctgt gatatagaaa ggccgatgaa
     3601 gtggcggtgg tcacaaaaaa taaataggtg gataggtggg gtgctaatgt cagcggcagc
     3661 atcacccaac gataataatg acaggactgg tgctataaat catttattta aatatgttaa
     3721 tgctattcgt gatgcaggtc agaagctaaa aaaaaggaat cgaaaactat attatttgat
     3781 taaaaatgga gttatttctt tgatttttgt ttggcttgct gtctcagtgt ttagatggct
     3841 gtgatttgtt tttttctatt gagtcataca taaatttggg tcttctatgc tcaatgtatg
     3901 gttatagata aaaatcgcct gctaaaaaat tctattgaaa tctttttagt aagaaaggcc
     3961 cgaatctgac gctcagcggg cctttcgtga agaagtgcgg cagtagacag atttcatctg
     4021 atcacgttca tgtgctgctg tgcaattaag cgtttctttc ggagctttca cggtatttcc
     4081 ttcacgaaac cggccgccat tcactcttgt gcatagccat gcagccgcgc agaaattatc
     4141 aggggcgttt ttcagggttt gggccgggga aaggtgccgg aaacggccct caggccgcag
     4201 aaacgcgctg aggggctctc agggggtgcg gccaagaagc gttatggtaa atacatatta
     4261 tggttattta ttaggagtaa ctctgggaga gtgggctatg ggatcagatg ttcaatggca
     4321 gatagataat ggttatttgg cgcaagttat gggggatttt tcgacagagt cgcctagctt
     4381 tgaaacgcca atgtttgggc gatattttga tacctttcaa gctgctatac atgttgcaaa
     4441 agctttccca gggagtacgt tgcagccatc agagtacgaa gataaaactg ggatgctaat
     4501 acgtcattat tatgtggatt gtcatccgta ttatgatcga ctccctcctg aaaaaagcga
     4561 taattttcag tatgatgagt acccttctgc tattcatgtg gctgcgtgta ttgatcaagc
     4621 tagcaaagaa ctcgacgaac tattgtttgg tggtggatat ggggaaaatg gtgaggggca
     4681 tgcaagcccg ataattttat attagtgtta ggggcggagg gatattatcc aatttgtaaa
     4741 atgatcttct tgagatcgtt
//
