LOCUS       NZ_CP126621             2987 bp    DNA     circular CON 10-MAY-2024
DEFINITION  Klebsiella pneumoniae strain K8 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP126621
VERSION     NZ_CP126621.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35344985
            Assembly: GCF_039566125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Stein,M., Cho,G.-S., Brinks,E. and Franz,C.M.A.P.
  TITLE     Complete genome sequence data from fresh produce 3rd batch
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Stein,M., Cho,G.-S., Brinks,E. and Franz,C.M.A.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2023) Microbiology and Biotechnology, Max
            Rubner-Institut, Hermann-Weigmann-Strasse 1, Kiel 24103, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-JUL-2021
            Assembly Method                   :: Unicycler v. v.04.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 119x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039566125.1-RS_2024_05_09
            Annotation Date                   :: 05/09/2024 19:04:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,194
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 38 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 16 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126621.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K8"
                     /isolation_source="Onion sprouts"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany"
                     /collection_date="2015-05-02"
     gene            41..232
                     /locus_tag="QPT69_RS00300"
                     /old_locus_tag="QPT69_00305"
     CDS             41..232
                     /locus_tag="QPT69_RS00300"
                     /old_locus_tag="QPT69_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1542..1790
                     /locus_tag="QPT69_RS00305"
                     /old_locus_tag="QPT69_00310"
     CDS             1542..1790
                     /locus_tag="QPT69_RS00305"
                     /old_locus_tag="QPT69_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017096688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029793011.1"
                     /translation="MKNVRMQFDLPEDRLDELDSLMKKCGISTKKELFNYALTMLEWAV
                     DESESGHEIAAIDRDSKQFYALRMPILKRVNRTSTAN"
                     /name="hypothetical protein"
     gene            1863..2234
                     /locus_tag="QPT69_RS00310"
                     /old_locus_tag="QPT69_00315"
     CDS             1863..2234
                     /locus_tag="QPT69_RS00310"
                     /old_locus_tag="QPT69_00315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024242345.1"
                     /translation="MSDSLDGFTEGDTSKEKQAVKQPKKFFSDVDDSADRRHLRWTKSG
                     VTLAMTILVAVMNWMVISMLYHVVDEELSLIRSGFMQADARTVTTSVYLALIAGTVAEV
                     SALFLIIVKSVFKNNNNDI"
                     /name="hypothetical protein"
     Typing          complement(2304..2729)
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.766"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catcagaacg tacagtaagc actttcgcgc cggggctttc atgaacaaac aacaaaagac
       61 tgccgttaac atggctaaat tcatccagga tcagtccctg cttcttctgg acaggcttaa
      121 tgagctggat ctggatcatg aggctgattt ctgcgagaaa ctccatgagg atgcggaacg
      181 cctttaccga agcctgtcag cgcgcctgaa tgagcagcag gacggtgcgt aatggctccg
      241 ttctgggtat taccggagta ataccggcat cgccttaatt ttcattgtct tagcgcttaa
      301 cattattacc ccggtaatac ccagaatccc tgtatttttt cttttttatt tatttttcat
      361 gcagttgcga tgaaagtcgc ggctgcgtta ggtgtatgac cattttgagg ttgaaaaata
      421 atcaaccatg gcgggtgatt tacgcaggtt aaggggagat cgtgacaaaa aaagcgttcc
      481 tcactgtatc gttcatgcct gcatgaacgg gttattcttc ctgactacag aattatatcg
      541 ctccttcagt tcatacatca cttttacaac actccttcag tctgtacatc acttttacaa
      601 cacttttgca tcacttttac atcatttgta catcactttt acatcattcc ttcagctcat
      661 acgtcacttt tacgtcagtt ccatcctctg tgccatttgt acatccgacc cccttcctga
      721 acgtgctgaa aacggttttt ccggctgtgc cggcaacgcc tcacggccac gcaataaatt
      781 gcgtccccgt tcaccgtttt cggataagaa gaacatcgga aacaggactt accccggctg
      841 aatggcgtga atttccgcgc tatgcacttg cgcgcatact catgcatgcc gtaaaaacag
      901 agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc ctgttttacc ggttttccgt
      961 cagaaacgcc ctgaggccgt tttagcggtg cgtacaatac ttgagttatg ttaaatgcac
     1021 ctaagatttg gatgattgtt tcatctcgtt acaactactg ttgtcttttt aacctatatc
     1081 gttttatact cgtaatatgc tccaattaca ctcgacaatg gaggtgtttg ggtgtaagat
     1141 acaatctgga gcccattacg aggtgtggtg ggtaaactgg agatttcacc tgtgagcgag
     1201 ttaactcaaa actgctcctc gacctccagg aacagtgagg actgcaccga cttcgaagaa
     1261 ctgcctccta aagagctttc tttgaaggat tggttctcag tctttactga ggtcgatgag
     1321 taataaccac catggaggag ttttcacaac ctcctccatg gtggggataa cctgcttgcg
     1381 gagaagagat aaggctaagc cttatccaaa ttaggagtat cataagtata tgaaacttaa
     1441 tttatttata ggtagagata tataaatacc aacggtccta atggtgaaga atgtgcagca
     1501 tagagtacaa ggatgacatg ctggcccctc ggagattagt tatgaaaaat gtacgtatgc
     1561 aattcgatct acctgaagat aggttggatg aactcgattc tttaatgaag aagtgcggca
     1621 taagtactaa aaaagagttg ttcaactacg cgttaacaat gcttgagtgg gctgtggacg
     1681 agtccgaaag tggtcacgaa atcgcagcta tcgatagaga cagtaagcaa ttttatgctc
     1741 tacgaatgcc tattcttaag cgagtgaacc gtacatctac tgcaaattaa cttatcactt
     1801 gatgtgttaa ggagaaggcc agcttaaact ggccttcttt ttttattcaa gagggtttga
     1861 ttgtgtccga tagcctagat ggatttactg aaggcgatac ctcaaaagaa aaacaagcgg
     1921 taaaacaacc caagaagttc tttagtgacg tcgacgattc agcagataga cggcatctga
     1981 gatggacaaa aagtggcgtt actctggcta tgacaatttt ggttgctgta atgaactgga
     2041 tggtcataag catgctatat cacgtagttg atgaagagct ttctctgata cgaagtggct
     2101 ttatgcaagc agacgcaagg actgtaacta ctagtgttta tttggctttg attgctggaa
     2161 cagttgctga ggtatctgct ttatttttga taattgtaaa gtcagttttt aagaacaata
     2221 acaatgatat atgaagttga ttccagtcgt tagatgtagt gtcacacatg gttttgttct
     2281 gtctgtcttg agatcactct aatcaaaatg taatctcttg ggactgaaac gaaaaaacca
     2341 cccggcaagg tggttttttc gaaggttcat taaccggcaa gtttctgaac cgtggtaact
     2401 ggctttcgca gagcacagaa accgaattcg ttttaacagt gtagccgtag tctgccttac
     2461 acttcaacac ctcaacgctt ctttgttctg cgaatctgtt acctgtggct gctgccattg
     2521 gcgttttaac gcgttttttc cgggttggac tcaaggtgat agttactgga aaaaacgcag
     2581 cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg
     2641 gatctgagtg tcaggcgtgg agtgagaaaa cgcggccata acagcggaat gacaccggta
     2701 aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat
     2761 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtccgattct gtgatgcttg
     2821 tcaggggggg cggagcctat ggaaaaacgc cccgccgggg cgtggccttt tagcccttgt
     2881 ccttagcttt cgttatgctt accgttacgt tatctgctta tcttccctcc gctcgccgca
     2941 gactcatgac cgaacggagt gagcgaatga gcgaggaagc ggaaaac
//
