LOCUS       NZ_CP126538             2175 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Citrobacter freundii strain CRNMS1 plasmid pCRNMS1-P3, complete
            sequence.
ACCESSION   NZ_CP126538
VERSION     NZ_CP126538.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35332011
            Assembly: GCF_030177735.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Fang,H.
  TITLE     Genomic and phylogenetic analysis of a Citrobacter freundii strain
            carring blaNDM-5 in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Fang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030177735.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 05:21:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,532
            CDSs (total)                      :: 5,411
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 39 of 179
            Pseudo Genes (incomplete)         :: 136 of 179
            Pseudo Genes (internal stop)      :: 23 of 179
            Pseudo Genes (multiple problems)  :: 18 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126538.1.
            Bacteria and source DNA available from Zhejiang Provincial People's
            Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CRNMS1"
                     /isolation_source="Patients"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCRNMS1-P3"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collection_date="2023-01"
                     /collected_by="F,HE"
     gene            complement(join(2044..2175,1..30))
                     /locus_tag="QOK75_RS27640"
                     /old_locus_tag="QOK75_27640"
     CDS             complement(join(2044..2175,1..30))
                     /locus_tag="QOK75_RS27640"
                     /old_locus_tag="QOK75_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045343414.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            717..953
                     /locus_tag="QOK75_RS27645"
                     /old_locus_tag="QOK75_27645"
     CDS             717..953
                     /locus_tag="QOK75_RS27645"
                     /old_locus_tag="QOK75_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1219..1644)
                     /locus_tag="QOK75_RS27650"
                     /old_locus_tag="QOK75_27650"
     CDS             complement(1219..1644)
                     /locus_tag="QOK75_RS27650"
                     /old_locus_tag="QOK75_27650"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_015328245.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     Typing          381..553
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(4..434)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.919"
                     /coverage="83.0"
                     /evalue="0.0"
ORIGIN
        1 gtagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
       61 ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga atgacaccgg
      121 tacaccgaaa ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg
      181 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct
      241 tgtcaggggg gcggagccta tggaaaaacg gcagcgccgc atcctttttc tgccccgttt
      301 gtcatggcat atgcctttgc tcttctccgt gtgcaggtaa cgcccgccgc agccgaacgg
      361 caggagcgtc cgcgactgag tgagcgagga ggcgtcatat cctctgaact tccattttct
      421 gccttacgcc cgcagctgca ttttcctctg tcacatgaag cactttcctg attaccgcac
      481 tcatgccaac ataataagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
      541 gcgccccgaa acccgctaaa tccactgacg cgcctgcggc ttgtccaacg cctcacggca
      601 ccggattaaa atccgctccc gttcaccgtt tctgactgcc tttcaccgtc gcttacgctt
      661 catcgttcct actgtgtctg gcagactgct gggactattc tcatacggag ataaacatga
      721 cgagacttac aaacctgacc cccgctgaga aaaagtttat cgacgacgct atagccgctg
      781 ccgaacgtgc ggccgggaag aagctcaatc agcctaaccg gcacatcgtt ctgaacagag
      841 cgcgtgcgca gattgagtcg cagcgttacg ccgaccgcca gcgtgcatta cgtgaggatg
      901 agcggcagca gtccgatttt gcctggtcac gtccgcgcgc gccgcgccgc tgattgcgga
      961 ttttgtcggt ttagtggtgc tgacgctgcg aaatcacctg cgattcaccc gcgtgcataa
     1021 cgattcatgc gctcaaaaat caccagagcg tttctgaacg tttttcagga ggtcatacgt
     1081 gttcttttgc gtattagggc ggagaaacgc cctgagagcc tctgaggcgg tgcgggcaac
     1141 gggtgttatg gtaaatccag ctgattggca ggtacaaaaa aagccccgta gggctttttt
     1201 tgtaatgcta cgaggaagct actgctgctt agaattcatt tcctcgatat gattgttcac
     1261 aatcgatctg aattgcattg gcgccttgat gtttggaatt ggagtgttgg ttccgccagt
     1321 gcctttaatc acgatagacc catagttaaa gattcgtccc agtatccctt gatgtatgcc
     1381 aaggctttca actttatcaa tacggagttc caccgtattg cgtcggatca aacccacttt
     1441 tgcgatgact ctttggttag ttaatgcaag ctccgttgac caaactctaa ggagcacagg
     1501 gattattata agtaacccta agccgtaaaa aggcagcaac aagattccga taattactgg
     1561 gatgattgtt ggaatccagg aggtttgagc gcgagatatt acggattcat ttcttgtcaa
     1621 tgattggtct acgtagcttc ccataaggtt atatccattt agaaaaaata ggatttttcc
     1681 ctttttaagg cgaatcagca atagaaactt tatgtctccg tggtaggtat acaaaattta
     1741 cggagagcgt agcgaccgtc gcacagttcg gttctttcct ttcgtgccgc aactggcttg
     1801 attctgtgat ggtggtggtg gctgctgcta tgtctcatat actcttggcg ttgcaatcct
     1861 ttatcccata tctgtcgaat atcacagatg atcttcttga gatcttgtcc gttgcgtcgt
     1921 aatctcttgc tctggaaacg aaaaaaccgc cctgcaaggc ggtttttcga aggttctcag
     1981 agctaccaac tctttgaacc gcggtaactg gcttggagga gcgcagtaac caaatctgtc
     2041 ctttcagttt agccttaaca ggcgcataac ttcaagacta actcctctaa atccgttacc
     2101 aatggctacc gccactggcg atttgtcgtg tctttccggg ttggactcaa gttgatagtt
     2161 accggaacgg gcgtg
//
