LOCUS       NZ_CP126062             7948 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain CS00491 plasmid
            p4_CS00491, complete sequence.
ACCESSION   NZ_CP126062
VERSION     NZ_CP126062.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35102991
            Assembly: GCF_030122725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Mellmann,A.
  TITLE     characterization of blaOXA-48 carrying plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2023) Institute of Hygiene, University Hospital
            Muenster, Robert-Koch-Strasse 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link v10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030122725.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:34:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,541
            Genes (coding)                    :: 5,380
            CDSs (with protein)               :: 5,380
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 87 of 161
            Pseudo Genes (incomplete)         :: 73 of 161
            Pseudo Genes (internal stop)      :: 17 of 161
            Pseudo Genes (multiple problems)  :: 16 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126062.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CS00491"
                     /isolation_source="anorectal swab"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p4_CS00491"
                     /geo_loc_name="Germany:Muenster"
                     /lat_lon="51.96 N 7.61 E"
                     /collection_date="2022-05"
                     /collected_by="Institute of Medical Microbiology,
                     University Hospital Muenster, Germany"
     gene            75..317
                     /locus_tag="QM175_RS27575"
     CDS             75..317
                     /locus_tag="QM175_RS27575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(663..1064)
                     /locus_tag="QM175_RS27580"
     CDS             complement(663..1064)
                     /locus_tag="QM175_RS27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1064..1255)
                     /locus_tag="QM175_RS27585"
     CDS             complement(1064..1255)
                     /locus_tag="QM175_RS27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1646..1786
                     /locus_tag="QM175_RS27590"
                     /pseudo=""
     CDS             1646..1786
                     /locus_tag="QM175_RS27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1755..2858
                     /locus_tag="QM175_RS27595"
     CDS             1755..2858
                     /locus_tag="QM175_RS27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_283903545.1"
                     /translation="MSLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCFYDVK
                     NPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYETETNQ
                     RLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQLKED
                     GVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILENLKKI
                     DDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSIILIDE
                     IENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIRLGRE
                     KNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            2858..3490
                     /locus_tag="QM175_RS27600"
     CDS             2858..3490
                     /locus_tag="QM175_RS27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            3856..4098
                     /locus_tag="QM175_RS27605"
     CDS             3856..4098
                     /locus_tag="QM175_RS27605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(4444..4845)
                     /locus_tag="QM175_RS27610"
     CDS             complement(4444..4845)
                     /locus_tag="QM175_RS27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4845..5036)
                     /locus_tag="QM175_RS27615"
     CDS             complement(4845..5036)
                     /locus_tag="QM175_RS27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5452..5565
                     /locus_tag="QM175_RS27620"
                     /pseudo=""
     CDS             5452..5565
                     /locus_tag="QM175_RS27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            5910..7025
                     /locus_tag="QM175_RS27625"
     CDS             5910..7025
                     /locus_tag="QM175_RS27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            7025..7657
                     /locus_tag="QM175_RS27630"
     CDS             7025..7657
                     /locus_tag="QM175_RS27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          1377..1748
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.186"
                     /coverage="99.0"
                     /evalue="1.38e-117"
     Typing          5158..5565
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
       61 gagtatgcgc gcaagtgcat agcgcggaaa ttcacaccat tcagccaggg gaagtcctgt
      121 ttccggcgtt cttctcctcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
      181 aagcgttgcc ggcgcagccg gaaaaccggt tttcagcacc ttcaggaatc gggttcgtcg
      241 acggccggca atcacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga
      301 ttcgaatcgt gaattgaatc aagagtcacc atgagcaaaa aacacccaaa gtgatgaatt
      361 gaatcaagag tcgattcatc ataacaatat gataataaag cgtgattcac atatgaatcg
      421 tgggcaattc gtcagcgatt cgccatagag tcgtccacga cttggatctg cactttgtaa
      481 cacatcaccg cacatttatt tttcattaac ccattgttgt aaataaggaa tctgcaaaat
      541 tttaattttg cgtggggtgt gggtattttt agtggtgagg agcgttcaga gatctgacga
      601 tttttgggaa ggttcaggaa ctccctggga tggccggaga ggaaaactga agcatgacgg
      661 ggtcaggatt ttttccagaa tttccaccat ggcgccgggg attcagttaa ttttgcctgg
      721 tgattttcaa gaaggagcat ggcctgtttc aggctgtcaa tgtgcgaatc cttggacaaa
      781 agtaaggtgt tcaggtgttc aatctcctgt ttcagatgac ggatcacctg ttcactctgg
      841 tcagcagagg ctgtatcacg ctgtccagag acctgtccag aatggtgttc acccttcggt
      901 tgctcaaagg ggccaaagac acgtaaaagc tctgaagtat ctaccatttt tgcattgcgc
      961 tcaccgctac aggtggacag gtcacctgct ttaattaacc tgtgcagtgt tgttctactc
     1021 ttacccgtca gtcgggcagc ctcactaata gacactctgg ccatcagtta ccatcctgta
     1081 atgcatccag tctcgaggac agtgtacgga aaagatgctc cgcatcatcg tggagtttct
     1141 cacaaaggtc ggcctcgaca tcgagatcaa gctcgttaag cttctccagc aggagcagag
     1201 actgattctg gatgaattta gccatgttca gcgctgtttt ctgttgtttc gtcataagag
     1261 gcctcagagc gtcagtggtc agtgtaggga tctcctcttt tccgcttcct cgctcactca
     1321 gtcgctacgc tcctgccgtt cggctgcggc gagcggcagg agcatgcgta gaaacgctcg
     1381 agaaaaccga gtaatcgcat ggaggaaaga aggaaaggcc gcacagcggc cgtttttcca
     1441 taggctccgc ccccctgacg agcatcacgg aatctgacgc tcaaatcagt ggtggcgaaa
     1501 cccgacagga ctataaagat accaggcgtt tccccctttc ctgcctttcg gtttaccggt
     1561 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     1621 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc
     1681 ctaatccggt aactatcgtc ttgagtccaa cccggaaaga cacgacaaag cgccactggc
     1741 agcagccaaa ggaaataagt ttaaagaact tcaaatgctt caatgagcta tatctaaagc
     1801 aattaaagac tttaaatatc atagctggga aaaacaacta cgggaaaaca tctatactgg
     1861 atgctatttt ttgtttttat gatgtaaaaa acccggctgt tttgttaaac attcaagcat
     1921 tcagaaaaga aatggcagaa ataaacaaaa acaagccatt ttgggttagt tactttcatg
     1981 atatggacac atcacaaaag atgtccatag tcattcgaga tgaacgctct gaagtaacac
     2041 aaacatatga aacagaaaca aatcagagat tagaatcaag tttgtctcta aacgttgaca
     2101 ccgtactatc taaccaaaac atacctcgtc aaactatcgg ttctcaggcc acagtaaact
     2161 ctttaaaaat aaatgtgacc gagacaccca atgcgaaaaa cagggtttcc aataagcttt
     2221 tctctatgca attgaaagaa gatggcgtag aaataaaatc cgaaataaaa aacgaaaggg
     2281 atgggattaa ttataaattt aagacagcaa caataataac aacatccaga aagataaaca
     2341 aagaggcaac aattacaaat gtatctagcc tactaacaca aaaaaggaaa aaagacatac
     2401 tagaaaactt aaaaaaaata gatgacagaa ttgtagatat agcaatatct gcgataggta
     2461 acaataagga aatatattta gacattggat ttagtgagct aaatgaaata tcaatgcttg
     2521 gcgagggtat cagtcgagca ttatctttta tcagctcagt gctggtacag gaaaactcaa
     2581 ttattcttat tgatgaaatc gagaacggaa tacattactc agtaattaaa gacataataa
     2641 aatcattaat ttcatctgca aaacaaaaca acaatcaaat atttgcaaca actcatagtc
     2701 aagatgtgat acgagcaata aacgaaatag atagcaaaaa tgaagatatc gcttatatta
     2761 gactaggaag agaaaagaac tccctaaaac caacagccgt acaattcaat atggatgatt
     2821 tctcctactc agttgagaac gactgggagg taagataatg aaagacataa ataatagcaa
     2881 aaagatcatt ttcgttgagg gtgatgatga ttttcatttt ctatgcaacc tacttgaaat
     2941 acaaggcatt accgatgttt ttgtcgaaaa aataaatgga aaatcaaaat taaaacaagc
     3001 acttagagca ttcaaaaaca ttgattcttt cgatgaaaaa gaaagtattt ttattatatt
     3061 agatgctgac acatcatttt ctgacacaga aagatcaata attgccacat tgcgagaact
     3121 acaaatctcc tcccccagct cgcataacga gatagctatg aatggcaata caaaaatttc
     3181 tttctttata atgccaggaa aagacaagca aggtgccatt gaagatctga taattgaaca
     3241 cgcaagcact cgagcgttat atgaatacat aatagaattt ttcgaaaaaa ttaaaaataa
     3301 taaagatgac atctcagcga gtgacaaaga gtacacttat ccaacaaacg aaaaaaaagc
     3361 taaggtacaa gtctatctta gctgcaatag agaatccgac tcaagaatag gaatatccat
     3421 gaaaaatgga ataataaaca cagcagacaa ttgctttgat gaaataaaag attttatttc
     3481 aaaaatataa atccaaatca agccagccct tcacgggctg gcttatcacc agggtgttat
     3541 taaacgccgg ataacgtata gaaagcatca tacactttag ttatcagcaa tctcaaacgt
     3601 cctaaagaca tcaccagatg ccaaaacaat gattgtgcag atctaatgta aaagacccag
     3661 cacaagatgc tcccttaagt tatattctct ctattcactt aaaacaattt aacataaaat
     3721 cataattgta cgcaccgcta aaacggactc aggacgtttc tgacgggaaa ccggtaaaac
     3781 aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt acggcatgca
     3841 tgagtatgcg cgcaagtgca tagcgcggaa attcacacca ttcagccagg ggaagtcctg
     3901 tttccggcgt tcttctcctc cgaaaacggt gaacggggac gcaatttatt gcgtggccgt
     3961 gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac cttcaggaat cgggttcgtc
     4021 gacggccggc aatcacgtta tggcaagtcg ccggacgact caccacacga ttcaccggcg
     4081 attcgaatcg tgaattgaat caagagtcac catgagcaaa aaacacccaa agtgatgaat
     4141 tgaatcaaga gtcgattcat cataacaata tgataataaa gcgtgattca catatgaatc
     4201 gtgggcaatt cgtcagcgat tcgccataga gtcgtccacg acttggatct gcactttgta
     4261 acacatcacc gcacatttat ttttcattaa cccattgttg taaataagga atctgcaaaa
     4321 ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgacg
     4381 atttttggga aggttcagga actccctggg atggccggag aggaaaactg aagcatgacg
     4441 gggtcaggat tttttccaga atttccacca tggcgccggg gattcagtta attttgcctg
     4501 gtgattttca agaaggagca tggcctgttt caggctgtca atgtgcgaat ccttggacaa
     4561 aagtaaggtg ttcaggtgtt caatctcctg tttcagatga cggatcacct gttcactctg
     4621 gtcagcagag gctgtatcac gctgtccaga gacctgtcca gaatggtgtt cacccttcgg
     4681 ttgctcaaag gggccaaaga cacgtaaaag ctctgaagta tctaccattt ttgcattgcg
     4741 ctcaccgcta caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact
     4801 cttacccgtc agtcgggcag cctcactaat agacactctg gccatcagtt accatcctgt
     4861 aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc
     4921 tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga
     4981 gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataaga
     5041 ggcctcagag cgtcagtggt cagtgtaggg atctcctctt ttccgcttcc tcgctcactc
     5101 agtcgctacg ctcctgccgt tcggctgcgg cgagcggcag gagcatgcgt agaaacgctc
     5161 gagaaaaccg agtaatcgca tggaggaaag aaggaaaggc cgcacagcgg ccgtttttcc
     5221 ataggctccg cccccctgac gagcatcacg gaatctgacg ctcaaatcag tggtggcgaa
     5281 acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc gtgcgctctc
     5341 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     5401 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     5461 ccccccgttc agtccgactg ctgcgcctaa tccggtaact atcgtcttga gtccaacccg
     5521 gaaagacacg acaaagcgcc actggcagca gccactggta actgatttag ggagttagtc
     5581 ttgaagttat acgccagtta aagctaaact aaacgaacag ttttggtgac tgcgctccct
     5641 ttaagccagt tacctcggtt catagagcgc caactctact gaacctttcg aaaaaaccgc
     5701 ccgccaaggc ggtttttttc gttcacagcc ggaagcttac ggctgctgcc agataatctc
     5761 aagaagaaag taaaaaaaat cgattaaaac acaagcaatc aaagttaatc tttgacataa
     5821 agtctagcta atcacaatga aagctgtaca ttttattaag gtactaacaa atcgtatggc
     5881 atataacaaa aacacgaaca gaggctatca tgataaagga aataagttta aagaacttca
     5941 aatgcttcaa tgagctatat ctaaagcaat taaagacttt aaatatcata gctgggaaaa
     6001 acaactacgg gaaaacatct atactggatg ctattttttg tttttatgat gtaaaaaacc
     6061 cggctgtttt gttaaacatt caagcattca gaaaagaaat ggcagaaata aacaaaaaca
     6121 agccattttg ggttagttac tttcatgata tggacacatc acaaaagatg tccatagtca
     6181 ttcgagatga acgctctgaa gtaacacaaa catatgaaac agaaacaaat cagagattag
     6241 aatcaagttt gtctctaaac gttgacaccg tactatctaa ccaaaacata cctcgtcaaa
     6301 ctatcggttc tcaggccaca gtaaactctt taaaaataaa tgtgaccgag acacccaatg
     6361 cgaaaaacag ggtttccaat aagcttttct ctatgcaatt gaaagaagat ggcgtagaaa
     6421 taaaatccga aataaaaaac gaaagggatg ggattaatta taaatttaag acagcaacaa
     6481 taataacaac atccagaaag ataaacaaag aggcaacaat tacaaatgta tctagcctac
     6541 taacacaaaa aaggaaaaaa gacatactag aaaacttaaa aaaaatagat gacagaattg
     6601 tagatatagc aatatctgcg ataggtaaca ataaggaaat atatttagac attggattta
     6661 gtgagctaaa tgaaatatca atgcttggcg agggtatcag tcgagcatta tcttttatca
     6721 gctcagtgct ggtacaggaa aactcaatta ttcttattga tgaaatcgag aacggaatac
     6781 attactcagt aattaaagac ataataaaat cattaatttc atctgcaaaa caaaacaaca
     6841 atcaaatatt tgcaacaact catagtcaag atgtgatacg agcaataaac gaaatagata
     6901 gcaaaaatga agatatcgct tatattagac taggaagaga aaagaactcc ctaaaaccaa
     6961 cagccgtaca attcaatatg gatgatttct cctactcagt tgagaacgac tgggaggtaa
     7021 gataatgaaa gacataaata atagcaaaaa gatcattttc gttgagggtg atgatgattt
     7081 tcattttcta tgcaacctac ttgaaataca aggcattacc gatgtttttg tcgaaaaaat
     7141 aaatggaaaa tcaaaattaa aacaagcact tagagcattc aaaaacattg attctttcga
     7201 tgaaaaagaa agtattttta ttatattaga tgctgacaca tcattttctg acacagaaag
     7261 atcaataatt gccacattgc gagaactaca aatctcctcc cccagctcgc ataacgagat
     7321 agctatgaat ggcaatacaa aaatttcttt ctttataatg ccaggaaaag acaagcaagg
     7381 tgccattgaa gatctgataa ttgaacacgc aagcactcga gcgttatatg aatacataat
     7441 agaatttttc gaaaaaatta aaaataataa agatgacatc tcagcgagtg acaaagagta
     7501 cacttatcca acaaacgaaa aaaaagctaa ggtacaagtc tatcttagct gcaatagaga
     7561 atccgactca agaataggaa tatccatgaa aaatggaata ataaacacag cagacaattg
     7621 ctttgatgaa ataaaagatt ttatttcaaa aatataaatc caaatcaagc cagcccttca
     7681 cgggctggct tatcaccagg gtgttattaa acgccggata acgtatagaa agcatcatac
     7741 actttagtta tcagcaatct caaacgtcct aaagacatca ccagatgcca aaacaatgat
     7801 tgtgcagatc taatgtaaaa gacccagcac aagatgctcc cttaagttat attctctcta
     7861 ttcacttaaa acaatttaac ataaaatcat aattgtacgc accgctaaaa cggactcagg
     7921 acgtttctga cgggaaaccg gtaaaaca
//
