LOCUS       NZ_CP126061             3561 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain CS00491 plasmid
            p3_CS00491, complete sequence.
ACCESSION   NZ_CP126061
VERSION     NZ_CP126061.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35102991
            Assembly: GCF_030122725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Mellmann,A.
  TITLE     characterization of blaOXA-48 carrying plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2023) Institute of Hygiene, University Hospital
            Muenster, Robert-Koch-Strasse 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link v10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030122725.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:34:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,541
            Genes (coding)                    :: 5,380
            CDSs (with protein)               :: 5,380
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 87 of 161
            Pseudo Genes (incomplete)         :: 73 of 161
            Pseudo Genes (internal stop)      :: 17 of 161
            Pseudo Genes (multiple problems)  :: 16 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP126061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CS00491"
                     /isolation_source="anorectal swab"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p3_CS00491"
                     /geo_loc_name="Germany:Muenster"
                     /lat_lon="51.96 N 7.61 E"
                     /collection_date="2022-05"
                     /collected_by="Institute of Medical Microbiology,
                     University Hospital Muenster, Germany"
     gene            712..936
                     /locus_tag="QM175_RS27545"
     CDS             712..936
                     /locus_tag="QM175_RS27545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            938..1582
                     /locus_tag="QM175_RS27550"
     CDS             938..1582
                     /locus_tag="QM175_RS27550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            1582..2058
                     /locus_tag="QM175_RS27555"
     CDS             1582..2058
                     /locus_tag="QM175_RS27555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2265..2378)
                     /locus_tag="QM175_RS27560"
     CDS             complement(2265..2378)
                     /locus_tag="QM175_RS27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            2794..2985
                     /locus_tag="QM175_RS27565"
     CDS             2794..2985
                     /locus_tag="QM175_RS27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2985..3409
                     /locus_tag="QM175_RS27570"
                     /pseudo=""
     CDS             2985..3409
                     /locus_tag="QM175_RS27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2266..2673)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 tttacgttct ataaatttcg tttttttaat tttatttata cagatccgac tctatgacga
       61 ctcgtcatag agtccccggc gagtcgtcat agagtcggta acgactcagg actataaaaa
      121 caataagtta acaacgaccc gactctagac ccgactcccc gtttggttgt tttttagacg
      181 tgacgactct gggtacgact ctagtgaaga gtcgccggtg aatcgtgtgg tgagtcgtcc
      241 ggcgacttgc cataacgtga ttgccggccg tcgacgaacc cgattcctga acgtgctgaa
      301 aaccggtttt ccggctgcgc cggcaacgct tcacggccac gcaataaatt gcgtccccgt
      361 tcaccgtttt cggatgggaa gaacgccgga aacaggactt accctggctg aatagcgtga
      421 attttcgcgc tatgcacttg cgtgcatact catgcatgcc gtaaaaacag agcctgcgcg
      481 tttctggcgg gttttcgggt ggtttgttgc ctattttacc ggtttcccgt cagaaacgcc
      541 ctgaggccgt tttagcggtg cgtacaattt tggtgttatg ttaaatcatg gccacaaaat
      601 tccataaagt gaccaatgta atgttaaatg agcattgtgg atgaaagatg ttgttttatg
      661 gtattatttt ttatgttcta aacttaaatt tatttctatc ggggtattat aatgagcaat
      721 aaagattctt tagaaagaaa agtaacacca agaaaaaatg aacctgtatt cccgatggct
      781 ctaagagttg ggatagctga agatatttgt tttttagagt tagtggactt tcctgaaaaa
      841 gaatcaggaa gggtttttta ttccatagct ctaaccaaaa aacatgcaaa gaaccttatc
      901 gatggattga ataaattcgt tgagtcaggt gattagtatg aaatacatta accactgcga
      961 tagtgaagat tattatatta ctaatgatag cattgatctc ggtgttcgtt atgatgtttc
     1021 tgattataat aagttagagc taaatgattt aagagatccg gatttgttta aagttttggg
     1081 tcgagcattt gttgttgttg agaattctgt ttgggagtct tggaatgatg atgttaaaac
     1141 attagattgc tctggtgacg gttatgagtc aattttaaaa cattacgaat cattagaatt
     1201 atcaggtgaa ttcttattgt tttttaaaaa aataagaagt gccatttctg tttataatag
     1261 agaatacgag ttctgcactg gtgacctaga accatctttg caggctattg ttcagttgat
     1321 taggtatgcc cctgaacttg ctgcaaaaaa agacgctcag atttactttg ataaaaaatc
     1381 agagtgtttt gggcttatat ataaacaaac taagaaaagc aaaggcacgc taaatttaat
     1441 agtgaaaaca aataacgaga ttattttttc ctttgttaga aaagtttctg gagtggttaa
     1501 ggttacaggt agggcatata ttggcgatag ccttgataac tccattgcta tcaaatcatt
     1561 atttaaaatg atggagtggt gatgactgtg ttatcaactt taaataacct caaatcaatc
     1621 ccttgtatta atgcatttac gggcagagca aataattcat atttatggcg ttatgttatg
     1681 gaagatcata aagatgagtt tggcgctaca actattaagt gtttttatct tagggaggaa
     1741 atttcagagg attatgtgtc tatttatcat tcaaataaag attattatga aggaatggta
     1801 agggatgtac ataaaagaat ccctgtaggg aaaaccaaac ctgccatata tctctcattt
     1861 gatgcagcgg aagtgatcga gcaagttaac aaaccagata aatgtattga tataaaggac
     1921 gaaggttacc ctcacttggg tttgtggtat ttatttgata ttaatgatga atttactttg
     1981 attgaggtga ctacaactat tttggaatta tcctcttttt ttcaaagaaa tgaaggtggc
     2041 gtggttgttc ctctttagcg aaaaccgtct tgacgtcatc aagtgtgacc tagtactctt
     2101 cttgaaaaag aaaaaccgcc tttgcggggc ggtttttctt aggttctaag agtttgcggc
     2161 tctatgaacc aaggtaactg gctagttaga ggagcgcagt aaccaaaatc gttctttcag
     2221 tttagcctta actggcacat aacttcaaga ctaactcctc taaatcagtt accagtggct
     2281 gctgccagtg gcgcttcgtc gtgtctttcc gggttggact caagttgata gttaccggaa
     2341 aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     2401 gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat
     2461 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa
     2521 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
     2581 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc cgctgtgcgg cctctccttc
     2641 tccccatcac gcgatcgctc ggttttctcg agcttttata cgcatgctcc tgccgctcgc
     2701 cgcagccgaa cggcaggagc gtagcgactg agtgagcgag gaagcggaaa agagaacatc
     2761 cctacactga gcactgacgc tctgaggcct cttatgacga aacaacagaa aacagcgctg
     2821 aacatggcta aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt
     2881 gatcttgatg tagaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc
     2941 cgtacactgt cttcgggact ggatgaatta caggatggaa actgatggcc agagtgtcta
     3001 ttagtgaagc ggctcggctt gttaaggtta gtcgccccac aatttacaag atgattaaca
     3061 gtgggaaact aagttacacc tctgttgtaa agcatgggaa aaatactaaa gttgttgata
     3121 catcagagct tattagggtt tttggctcgc tttatggtgg gggggcgaca ctgtaaaaca
     3181 tgatgtaaaa ggtgacgcag gcgctacagg ggttaacatc tctggtttac atgatttaca
     3241 gcataaaatt gctttgcttg aagctgaaaa cgatggtctt aaaggggctg ttaaagctcg
     3301 tgatgaacat atcgattctc ttcgccaggc tatgcagctt ctggaacaca agctcgaacc
     3361 ctctccacag gttcattctc cgtggtggaa attctggaag aaatcctgac cccgtcatgc
     3421 ttcagttttc ctctccggcc attccgggga gttcctgaac cttcccaaaa atcgccagat
     3481 cgctgaacgc tcctcaccac taaaaatatc cacaccccac gcaaaattaa aattttgcag
     3541 atttgtattt tatatcagta g
//
