LOCUS       NZ_CP125869             3125 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Staphylococcus aureus strain 19-02247 plasmid p19-02247_2, complete
            sequence.
ACCESSION   NZ_CP125869
VERSION     NZ_CP125869.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35074235
            Assembly: GCF_038789195.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Schuermann,J., Fischer,M.A., Reemtsma,T., Karte,J., Staerk,H.-J.,
            Strommenger,B., Werner,G., Layer-Nicolaou,F. and Schuster,C.F.
  TITLE     The genes mgtE and spoVG are involved in zinc tolerance of
            Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Schuermann,J., Layer-Nicolaou,F., Schuster,C.F., Fischer,M.A. and
            Werner,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2023) Unit 13: Nosocomial Pathogens and Antibiotic
            Resistances, Robert Koch Institute, Burgstr. 37, Wernigerode 38855,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-MAR-2023
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: 19-02247_RKI_Sau
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 111.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038789195.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 23:48:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,803
            Genes (coding)                    :: 2,721
            CDSs (with protein)               :: 2,721
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 44 of 82
            Pseudo Genes (incomplete)         :: 41 of 82
            Pseudo Genes (internal stop)      :: 17 of 82
            Pseudo Genes (multiple problems)  :: 19 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125869.1.
            Bacteria and source DNA available from Robert Koch Institute, Unit
            13 Nosocomial Pathogens and Antibiotic Resistances, Burgstr. 37,
            Wernigerode 38855, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="19-02247"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="p19-02247_2"
                     /geo_loc_name="Germany"
                     /collection_date="2019"
                     /collected_by="Robert Koch Institute"
                     /genotype="ST3586"
     gene            complement(306..494)
                     /locus_tag="QM318_RS14415"
                     /old_locus_tag="QM318_14415"
     CDS             complement(306..494)
                     /locus_tag="QM318_RS14415"
                     /old_locus_tag="QM318_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1077..2072)
                     /locus_tag="QM318_RS14420"
                     /old_locus_tag="QM318_14420"
     CDS             complement(1077..2072)
                     /locus_tag="QM318_RS14420"
                     /old_locus_tag="QM318_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2524..3105
                     /locus_tag="QM318_RS14425"
                     /old_locus_tag="QM318_14425"
     CDS             2524..3105
                     /locus_tag="QM318_RS14425"
                     /old_locus_tag="QM318_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          complement(843..1007)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1078..2072)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttattgacg ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa tggtcggctt
       61 aatagctcac gctatgccga cattcgtctg caagtttagt taagggttct tctcaacatc
      121 aataaatttt ctcggcataa atgcgtcgtc tattttttat tatcttcatg ataatcatat
      181 ttttcttcca tctcgtcaaa agcatcatct ttatagaaaa acaaaaagta agcaacaaca
      241 ccaataggaa taaaaaaaca gtgtgaaaac aagaagaaac tcgtttcaaa atcccctcaa
      301 atattctaat tattcttttt gaaattcata accttaacaa ccaaataaat agcatttatt
      361 cccatcaaaa taacatacgg aattaatata tataaatttt ccaaatgaga tataaaaatt
      421 aatccaagta aaaagaaaac atttaaaata gttgctataa cgtaaccttc atctaacact
      481 gtgtttttat tcaaaataaa cactcctttt ttaaaaaact ttatcacaag acttactcaa
      541 attataaata cctttatttt gagttttaag ggggtatttt aaaggtttag ggggtatcat
      601 ataggttttt aacttaaaaa tatatacaag ctcttaaaac gcaaatatga gccaaataaa
      661 tatatggtaa tttcacaaac aaaaatttca gcaaaaccag tgactaattt tttaacactg
      721 cccatttaca tgcaaattaa aaattagcat aaaatccttt aaaacttcca catgtttttc
      781 taaattcctg cagggcgttt tttatcaaaa atcgagaatt tttaattttt ctctaaaaac
      841 tccgtaagag cataaggaga atttgctatc attcaaaaat gataggtctg gcaaagccag
      901 aaattccagg ggttttaagg tattttaaag tgatatcata caaagctgta ctgtcttatt
      961 tttgtgacac aatatattgc gtttgtctta tttttgtgac actacataca gcatttgtca
     1021 caaaaataag acagtacttt tttataaaaa atcaacaaaa aagacattag tcaacactaa
     1081 tgtcttaaat aataattttg cttttcgaaa ttccaaattg cggtaattga atgtgctttt
     1141 tcttcttcgt ctgttgtttt atcttcatca cttgcattaa tcaaattacc atcttcgaca
     1201 tcgtctaagt ttaaaatttt atgtttttgt ttaagcaatc caccataact taacatacgt
     1261 tttcgataca aacctttttc taaatcactt acaatttctt gatttttttc atcatcatca
     1321 gttaaaaaat cagatgattt aaccgaatat tttgaggtct ctttgattgc cgattcaatg
     1381 tctttatcgc cttttctatt cggtttgatc gctttaacat tagcaacagg tcgatagtca
     1441 acttgtaatg ctctttgcca taaattaacc cattcttctt gagttatata attctctttt
     1501 tttctaaaat atgcattttc aacacataac aaaacatgca tgtgctgatt ataactaccg
     1561 tcatttttat taacggtaac ttctgttgaa cgcataaatc caacaagatt ttgtttaacc
     1621 tttttatatc tactcaacct atcaaatgct ttagttagat gcttcaaact ttgttctaaa
     1681 gtatctccat ctatcgcatt ttttgttgaa agtgttaaaa acaaccaacg tgcttttggc
     1741 ttttccttaa ttactttttc aatcactttt tgagcttgat aactattttt catagcacgt
     1801 ctccaattac aaaccggaca taattttgat ttacaaaacc atgtcttatg taacttcaaa
     1861 tagccttcat cagttggctt aaattctaaa atattaccac attgttttac attaaaagcc
     1921 tttttgattt ttaaaatttc tagtatatct gcatagctta cattatctat tttcttttct
     1981 ctccatgggc gttgtttccc acttttcgtc atatctttta acgttttatt atcttgattt
     2041 tcggttatac ttctagtagt attatattgc atataaaaag acccccagta ttatttaaga
     2101 ttcgacacct taataatata actggggttt tttattgtca aattttcctt tcacgcacaa
     2161 aaacacgttt aaacactgta atatcaacag ttacagctat atatataaaa tcgagttatt
     2221 tctatatagt atcaagacaa gaagaaactc gttcacactc gtttcaaaaa acaacctatt
     2281 ccttcttctt tttatgaccc tctaaaccta aataaattaa tgtgaaaacc aatgaataaa
     2341 taaaactttc taaaaagtca aaaggattat caaataaaat cgaattaaca aaattaaaaa
     2401 ttaaaaatac agataataaa ataatcaaaa agtaaaaaaa cttattcata ttaaactcct
     2461 ttgtttttat atgttaatat tatattgaat tcatttaaat aacaaaacaa gggggcattt
     2521 acaatgaatg caattcaaaa cagtatatca ctaagaaaga ttttagtagt atctttaagt
     2581 attttaacca tactttcaat ggtcttggat tttaatttca aagaagctca agcccaaaac
     2641 aaaaataaca ttgcagataa aaacgttgaa actttaaacg aaaaagaaat tgaaaaagaa
     2701 ctaaaataca tatatggcaa aattattatt ttagataaag acggaacagc taaagatgtt
     2761 aatctagaaa atgccaaatc tcgatatgga tatgttcctg aaggattcca aaaactaaaa
     2821 aacgacatag agaataaaaa gaaaactagt tcaatatcac ctagagcagt tggtgggaac
     2881 tacaaaaata gcggtgactg tttctattct gaagttttaa actcatatgg agaactttta
     2941 actggaaaca taatcacagc agtttttgaa gatgttaaag ccaaaaactt aaagggcgta
     3001 gcaaaaaaat tagctaaaat tgggataaaa ggaaacctag ctggaatcgc agctactatg
     3061 gtttcgaagg atgtacaatg taatcttaaa tacgggttac tttagatact atttattcca
     3121 attgc
//
