LOCUS       NZ_CP125866             1373 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Staphylococcus aureus strain 19-00012 plasmid p19-00012_3, complete
            sequence.
ACCESSION   NZ_CP125866
VERSION     NZ_CP125866.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35074234
            Assembly: GCF_038789165.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Schuermann,J., Fischer,M.A., Reemtsma,T., Karte,J., Staerk,H.-J.,
            Strommenger,B., Werner,G., Layer-Nicolaou,F. and Schuster,C.F.
  TITLE     The genes mgtE and spoVG are involved in zinc tolerance of
            Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Schuermann,J., Layer-Nicolaou,F., Schuster,C.F., Fischer,M.A. and
            Werner,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2023) Unit 13: Nosocomial Pathogens and Antibiotic
            Resistances, Robert Koch Institute, Burgstr. 37, Wernigerode 38855,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-MAR-2023
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: 19-00012_RKI_Sau
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038789165.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 23:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,775
            CDSs (total)                      :: 2,693
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 41 of 88
            Pseudo Genes (incomplete)         :: 44 of 88
            Pseudo Genes (internal stop)      :: 24 of 88
            Pseudo Genes (multiple problems)  :: 18 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125866.1.
            Bacteria and source DNA available from Robert Koch Institute, Unit
            13 Nosocomial Pathogens and Antibiotic Resistances, Burgstr. 37,
            Wernigerode 38855, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="19-00012"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="p19-00012_3"
                     /geo_loc_name="Germany"
                     /collection_date="2019"
                     /collected_by="Robert Koch Institute"
                     /genotype="ST398"
     gene            450..629
                     /locus_tag="QM319_RS13870"
                     /old_locus_tag="QM319_13855"
     CDS             450..629
                     /locus_tag="QM319_RS13870"
                     /old_locus_tag="QM319_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019835937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031920706.1"
                     /translation="MKEVSDLEEKNERKRMNITLPPELIEELEKVNKKYKINKSKQLET
                     LVRKYLRKEYGDLG"
                     /name="hypothetical protein"
     gene            714..1277
                     /locus_tag="QM319_RS13875"
                     /old_locus_tag="QM319_13860"
     CDS             714..1277
                     /locus_tag="QM319_RS13875"
                     /old_locus_tag="QM319_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_106095103.1"
                     /translation="MAKYTKSRTWGVVVYPESAPENWVEILDESRIQFAVSPLHDKDVN
                     PDGEIKKSHWHVILCWDGPVTDVAVKKLTDKIEAPNPIKLESVRGAYRYFTHMDHPDKY
                     QYDDKEIKVFNGFDISSYIALTKEERYEAITAIIRYINENKVTEYLDLLNELMTHDYSL
                     FKVACDNTILFTSVIRSNRHKTDR"
                     /name="replication protein"
ORIGIN
        1 agtgagtggc tagcaaagcc agtgcttgcc aaacactaac ttatgcaaat aagtctagtg
       61 tgttagactt taacgatttt aaagggactg tgactttatg ccgagaaaac ttttgccgag
      121 caaagaaata atgatagcca cttcagtggc gattggtgcc ggttttaagt tggattaatt
      181 ccaacttaac aaccaatcca aatcattact ttcgatatcg tgaaggcaaa aggaaattgg
      241 aataaatact taaacagatc tgatgaatat cagttctgtt ttttaggttt tgaacttgga
      301 atttggacct agatttttga tgatttttag aggattttcc taggggggta ctacgacccc
      361 ccctaggggt gcattgtgca atgtgcaaat ttttgtgtat gtacttgact ttatacacat
      421 aaaagcgtat tttcgacata tatctaaaaa tgaaggaagt gagcgatttg gaagaaaaaa
      481 atgagcgaaa aagaatgaat attacattac ctccagaact gattgaagaa ttagaaaaag
      541 tgaataagaa atacaaaatt aataagtcaa aacagttgga aacgttagtg agaaaatatc
      601 ttagaaagga gtatggtgat ttgggataaa aaaagaacct tgcggtaaca aggctctttt
      661 tgaatatact catgacatga cgtggcatga tatgcaatca ccaatataat agcatggcaa
      721 aatatactaa atcaagaaca tggggagtag tagtataccc agaatcagcg ccggaaaatt
      781 gggttgagat tttagacgaa tcgagaatac aatttgcagt ttcgcctttg catgataaag
      841 atgtaaatcc tgatggagaa ataaaaaaat cgcattggca tgtgatttta tgctgggacg
      901 gtccggtaac agatgttgca gttaaaaaat taacagataa aatcgaagca cctaatccga
      961 taaagctaga aagtgttaga ggagcttatc gctattttac gcatatggat caccccgata
     1021 aatatcagta tgatgacaaa gaaattaaag tatttaatgg ttttgatata tcttcttata
     1081 ttgcgttaac taaagaagag cgttatgagg caattacagc gattataaga tacattaacg
     1141 aaaataaggt tactgagtac ctagacttgc taaatgagct tatgacacat gattatagcc
     1201 tgtttaaggt ggcttgtgat aatacaatac tgtttacttc tgttattcgt tcaaatcggc
     1261 ataaaactga tagataagct agccattgtg gctagctttt tgcgttagca aaaataaggg
     1321 gtttggggag tatccccaac aagcaggcga gcagacacga atgagcgtta gcg
//
