LOCUS       NZ_CP125209             3511 bp    DNA     circular CON 09-APR-2024
DEFINITION  Klebsiella pneumoniae strain 50 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP125209
VERSION     NZ_CP125209.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790390
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 111x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038010595.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 16:05:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125209.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="50"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(<3404..3511,1..6))
                     /locus_tag="P4G27_RS00005"
                     /pseudo=""
     CDS             complement(join(<3404..3511,1..6))
                     /locus_tag="P4G27_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            627..968
                     /locus_tag="P4G27_RS00010"
                     /old_locus_tag="P4G27_28905"
     CDS             627..968
                     /locus_tag="P4G27_RS00010"
                     /old_locus_tag="P4G27_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1177..>1605
                     /locus_tag="P4G27_RS00015"
                     /old_locus_tag="P4G27_28910"
                     /pseudo=""
     CDS             1177..>1605
                     /locus_tag="P4G27_RS00015"
                     /old_locus_tag="P4G27_28910"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1646..2143
                     /locus_tag="P4G27_RS00020"
                     /old_locus_tag="P4G27_28915"
     CDS             1646..2143
                     /locus_tag="P4G27_RS00020"
                     /old_locus_tag="P4G27_28915"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            2150..2365
                     /locus_tag="P4G27_RS00025"
                     /old_locus_tag="P4G27_28920"
     CDS             2150..2365
                     /locus_tag="P4G27_RS00025"
                     /old_locus_tag="P4G27_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2736..2942
                     /locus_tag="P4G27_RS00030"
                     /old_locus_tag="P4G27_28925"
     CDS             2736..2942
                     /locus_tag="P4G27_RS00030"
                     /old_locus_tag="P4G27_28925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064408406.1"
                     /translation="MLITGVRVMLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYM
                     ENPTSANYKIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          369..542
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2..402)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.02"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(3186..3510)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.29"
                     /coverage="99.0"
                     /evalue="1.17e-59"
     Typing          959..2481
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
       61 gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
      121 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      181 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
      241 aaaacggctt cgccgcggcc ctatcgcttc cctgttaagt atcttcctgg catcttccag
      301 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
      361 cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct gacgcgccgg
      421 tgccgccttt tttctcctgc cacatgaagc acttcactga catcctcatc cgtgccaaca
      481 tagtaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa
      541 ccccactgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg ctttcccgtc
      601 cgtcacggcg ttatcaggag gcgattctgg cagataaacg caacaagatg ctcacgatgt
      661 gggtgacgga agacgaacac cggcggttgc ttgagcggtg cgacggaaaa caactggcgg
      721 cgtggatgcg ccagacctgc cttgatgaga aaccgacacg cgccggcagg cttccctcga
      781 tttcgcccgc gctgctgcgt cagcttgccg gcatgggcaa taacctgaac cagatcgccc
      841 ggaaagtgaa cgccggtggt gccggtcatg accgggtgca gatcgtggca gcgctgatgg
      901 cgatcgatgc cggtctggaa cggttgcggc atgccgtact ggaaaaaggg ccggacgatg
      961 attgttaaat tccatccccg gggtcgcggc gggggcgccg gtcctgtgga ttatcttctg
     1021 ggtaaagacc ggcagcgtga aggtgccagc gtcctgcagg ggaagccgga agaagtccgg
     1081 gagctgattg atgcgtcgcc gtatgtgaaa aaatatacct ccggggtgct gtcattcgcg
     1141 gaggcggacc tgccgccggg tcagcgtgaa aaactgatgg ccagctttga gcgggttctg
     1201 atgcccggac tcgataaaga ccagtacagc attctgtggg tggaacacgc agacaagggt
     1261 cgtcttgagc tgaattttct gatccccaat accgaattac tgacggggaa acgcctccag
     1321 ccgtattacg atcgtgccga ccggccgcgc atcgatgcgt ggcagacagt ggtcaacggc
     1381 aggctggggc tacatgatcc gaacgacccg gagaaccggc gggcgctggt cacgccttca
     1441 ggactgccag aaacgaagca ggaagccgca gagacgatta cacggggttt gctgatgtta
     1501 gcctcatcag gagagctgaa aacgcgtgat gacgtcacag gggcgctcac agcggccggt
     1561 tttgaggtgg tcagaaccac aaaaaacagt atcagcattg ccgacccgga cggggggcga
     1621 aatatccgac tgaggggagc catttatgag cagtctttta acggcctcga gggacttcga
     1681 ggagaaatcg aaagcacagc agcagcttac cgaagagagg ctgaaagccg cattcagcaa
     1741 gcacgagaac gttgtcaaat cggaactgaa cgcaagcgcg aagagaatca acgacgccat
     1801 cagcgcccac gaacagggga tgaacgcggc gatgcagtcg aaccggctga gcgtgctgcg
     1861 aatggtcggg cggacgtggc tgaccatcac catggtgtca gggctgctgt tcgccagcct
     1921 gagcggggtg ctgtggtacc aggggaaccg gatagcggcg aacctggcgg aaatcaggca
     1981 gcagagagat acgctgtcga aactgcacat gcagacgtgg ggcgtgacct atctggagaa
     2041 ccgcaacgga cggtttctgg tgctgccgga ggggatgaaa gccgaaacgg gccggacggt
     2101 ggacaacaag acgcggaacg cggtgaagct ggtacgggag tagagcagaa tgacggaact
     2161 ggaaaaacaa ttgctgagcg cgttagagca gctacagcag gactactcga gaaggctgga
     2221 cgagtgggag agcgccttcg aggaatggcg gaagatgtct ggtcttatgc aacgggagaa
     2281 cgcggcgctg agcgagcgcg tgtcgggctt gagcaggcag gtgcagagtt tgagcgagca
     2341 gctgcgccgg ttatcgacag gctgaatgag attgagcaac agcgatacgg gcaaacaacg
     2401 gaaaaggaac cagagaagac agcagatctg gtaccggaac ggcaacgcac attatctcat
     2461 ggtgacaggg agtatggaga ctgataaacg cccgtctcag gtggcctgag cgtgacgggg
     2521 atcatggcca ttttctgcga tttcagcaca cagagagtct tttttaaccg aaatcaccgg
     2581 ctatgcagtg atgtgaataa gtgatgcata tgcgtaaaat aaatcaggtg cgtttctggt
     2641 ggttttcggt gtacttgcca ccaccttttg cccgtcagcc atcagaaacg ccctgagtgc
     2701 ctccggcgcg gtgcgggtaa gagatgttat gtttaatgtt gataacagga gtaagagtga
     2761 tgcttacatt tattggtgta gtggtagtcg ccgttattgc atttaaatta tttatgcttt
     2821 tttttagttc atccgaatct gccgtcgaac gtgctacccg taaatatatg gagaacccta
     2881 cgagtgctaa ctataaaata atggtggcta cacgcatgag aaatgagaag aaaagagggt
     2941 gatatatgtt tgagtttatt ggttttttat ttttactttt ggtgtgttat gtctttatac
     3001 aaatggttgc tgtcagggtg tttcctgaat atggagtacg gaaagcggag gaacgatacc
     3061 gtcgaaatcc atcagcagaa aatgaggatc tgttatggag agctagaagt cgtataacta
     3121 aacgcaagta atgtttccta agtgtgttga taggcaaaca tggtgggagt tactactctc
     3181 ggagttaatg atcttcttgt gatcctgtcg tttcagtcgt aatctcttgc tctgaaaacg
     3241 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt ctgttgggga actgcttaac
     3301 caggtaactg ggtatacgag accgcagata ccaaaactgt tctttcagtg tagccgcagt
     3361 caggcccata cttcaagacc tctgaatctc tctctcgtat atcccagtta ccagtggctg
     3421 ctgccagtgg cgctttgtcg tgctttctcg ggttggactc aagatgatag ttaccgggta
     3481 aggcgcagca gtcggactga acggggggtt t
//
