LOCUS       NZ_CP125205             5010 bp    DNA     circular CON 09-APR-2024
DEFINITION  Klebsiella pneumoniae strain 50 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP125205
VERSION     NZ_CP125205.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790390
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8
            Genome Representation        :: Full
            Expected Final Version       :: No
            Genome Coverage              :: 111x
            Sequencing Technology        :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038010575.1-RS_2024_04_08
            Annotation Date              :: 04/08/2024 16:08:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="50"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(4750..5010,1..645))
                     /locus_tag="P4G27_RS00005"
                     /old_locus_tag="P4G27_28810"
     CDS             complement(join(4750..5010,1..645))
                     /locus_tag="P4G27_RS00005"
                     /old_locus_tag="P4G27_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            complement(893..1168)
                     /locus_tag="P4G27_RS00010"
                     /old_locus_tag="P4G27_28815"
     CDS             complement(893..1168)
                     /locus_tag="P4G27_RS00010"
                     /old_locus_tag="P4G27_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(1149..1394)
                     /locus_tag="P4G27_RS00015"
                     /old_locus_tag="P4G27_28820"
     CDS             complement(1149..1394)
                     /locus_tag="P4G27_RS00015"
                     /old_locus_tag="P4G27_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            1631..1792
                     /locus_tag="P4G27_RS00020"
                     /old_locus_tag="P4G27_28825"
     CDS             1631..1792
                     /locus_tag="P4G27_RS00020"
                     /old_locus_tag="P4G27_28825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            complement(2592..2993)
                     /locus_tag="P4G27_RS00025"
                     /old_locus_tag="P4G27_28830"
     CDS             complement(2592..2993)
                     /locus_tag="P4G27_RS00025"
                     /old_locus_tag="P4G27_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3395..4009
                     /locus_tag="P4G27_RS00030"
                     /old_locus_tag="P4G27_28835"
     CDS             3395..4009
                     /locus_tag="P4G27_RS00030"
                     /old_locus_tag="P4G27_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(4046..4753)
                     /locus_tag="P4G27_RS00035"
                     /old_locus_tag="P4G27_28840"
     CDS             complement(4046..4753)
                     /locus_tag="P4G27_RS00035"
                     /old_locus_tag="P4G27_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
ORIGIN
        1 caggttgtat gcgcaagcgc gttcgtagca ggcttgcagc cactgctcat actcaggcca
       61 gccctgacgg atggcgcgat atgcccactt acgggttttg tcgaacaacg tgcagttacg
      121 gcctaagccg tagtcggcaa cgatttcctt gtcattccct gtgttcagat cgagaaagtc
      181 ggccagccag tccagagtgt agagcttcgg ctgccacacg gcgattttcc agtggttgtg
      241 gttcggattt ttgcagatga gccccgaata tccagcgtca gcctctaatt ttttacgtag
      301 cgcattctcc acggaggcgg cgtagcgtaa cggttttatt ttgccgtccg gcgcggtgcg
      361 tatcgagttt tccagcgcgt acagcaggtg cgcgtgtccg ttttcggggt tagttatggt
      421 cagcgtcggt gcaggcgcgt tgcggtcgct ccagtcgatg gaggaaccgg cgcggtcaac
      481 gtcaaatccc agccagaaca tggcgtgagg ctgattgaac tggatatatt tcgccagaat
      541 agcgcgttcc ttaccggcga tacggacacc aaagtgcaga tcgtccgaaa agtacggctt
      601 gtgtggtaag cgttcgttga aaagttcgag tgcggcgtta tgcaatgtgc cctgcgctta
      661 tggcgggctg ttgtctggca cggttcatgt gctaatatcc tttcaaggtt tcgacctagc
      721 cctggttatc atgcgatccg ccaagatatg acatgatttc cggggctttg ttttttctga
      781 ggctaacgtt acaccgactt tgcctttgtg tcgatctgac agcgacttac cctaacatcc
      841 gttgtacgcc ttgcatgaaa agcccgcatc aagcgggcta tatcgacttt tgctatttgt
      901 caccatagtg ataacgggct gcgtagatga tgatttctcc gttctggatt gaatagacca
      961 gacggtggct ctgatcaatc ctgcgggagt agagtgccgg atctttgtgg tgccgcagcc
     1021 gttcgggttt tcctatacct gtcagcggat cagcctcgat gctgagcaac agttgccgga
     1081 tacgctcaaa cttctttttg tcagtatcac gccagtagtt aaggtcatca gcgccattcc
     1141 tagtgaactt aatattccac ttcgacaaat tcgccgcgct ccgcctgttt cattgactca
     1201 ttgatatgct ctgcgttcgc cggattgctg aacagataga ccgtttcgat tagcgcctca
     1261 tactctcccg catcgatcat aacaatatca ggcgcatcac ggcgcatgac acgtaccggt
     1321 tcagcatcat cagtgacgcg cttcatcgtc tcggcaaaat gtttacgggc atcagtaaaa
     1381 ttgattgcat gcatgttgtt aacccccatt tgttggggct tagcatagaa catgtacgca
     1441 aataagtaca taaagatatt tccctaaaaa cccacaacca aatcagctca aaatgtgaac
     1501 tcaatcgaat aaccacaaaa acaacaagtc aatacattga agcataattt caagcaatta
     1561 catttatgaa gtgcggcaca atttcgtacc gctaggagct tagctcctaa aacgagcaaa
     1621 ggaaaagtaa atggcaaaac attggtctgg gcaagaatgt ccaaaaacag gtacttacgg
     1681 tcaatatcat gataccgaca actcatacgc tggttctgaa tatgatcgcc atgtgagcaa
     1741 gggtgataag ttccctccga gcaaaaacaa ccaccacttc caagaaaaat aaaatatctt
     1801 cagaagtcta aactaaaagc cctctggggc tttttttgta cctgccattc acttgattta
     1861 ccataacatc cgttatcagc accgcctgag atgccctcag ggcgtttctg acgggaaacc
     1921 ggtaaacaag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
     1981 ggcatgcatg agtatgcgca caagtgcata gcgcggaaat tgacaccatt cagccagggg
     2041 aagtcctgtt tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc
     2101 gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt ttcagcacgt tcaggaatcg
     2161 gttcgtccac ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc
     2221 cccggcgact cttcactaga gtcgtaccca gagtcgccac gtctaaaaaa caaccaaacg
     2281 gggagtcgga tctagagtcg ggtcgttatt aactcattgt ttttataggc ctgagtcgtt
     2341 accgactcta tgacgactcg ccggggactc tatgacgact cgtcatagag tcggatctat
     2401 ataaataaaa tttaaaaaac gaaatttcag taaagtaagc tgctgatatt aaatacaaat
     2461 ctgcaaaatt aaaattttgc gtggggtgtg ggtatttttc gtggtgagga gcgttcagag
     2521 atctgacgat tttcgggaag gttcaggaac tccccggaat ggccggagag gaaaactgaa
     2581 gcatgacggg gtcaggattt tttccagaat ttccaccacg gcgccggggg ttcagttaat
     2641 tttgcctggt gattttcaag aaggagcatg gcctgtttca ggctgtcaat gtgcgaatcc
     2701 ttcgataaaa tcaatatgtt aaggtgttca atctgctgtt tcagttgacg aatgacatgt
     2761 tcactctggt cagcaggcgc tgtatcacgc tgttcaggta cttgtcgagt aaggtgttca
     2821 ttcttcggtt ggtcaaacgg tccgaaaaca cgtaaaagct ctgatatatc gaccattttt
     2881 gtatttcgtt caccgctaca agtggacagt tcacctgttt tgattaacct gtgcagcgtt
     2941 gtccggctct tacccgtcag ccgtgcagcc tcactaatag acactctggt catcagttat
     3001 catcctttaa tacattcagt ctcaaggaaa caggggccag cttacgacat actggtccag
     3061 aagtgatgtg tttttcagaa gggcgatgaa acacaggagt gaccgtgcaa aatgcaggca
     3121 acaaatgact ttccgcttcc tcgcgcattc gctcactacg ttcggtcata agactgcggc
     3181 gagcggtacg taagaataaa gtgaggtgat agctctcttc tcaaggcgtt tagggaagca
     3241 acgagcccgc cctaaaccag tcatggcgag accttgctga agggacccta tgtgtgctgg
     3301 ctgagaaaaa aagagctctc taaaagagag ctgttatctg atataggttc aagtgcaaaa
     3361 tagagtgcac acaaacaaaa aaaggacaat aaaaatggga aagcataagg tattcattag
     3421 ctaccaccat gcgaatgatc agtactacaa aaacgcactt gaaaaaatga atgaggaaca
     3481 tgaaatattc gtgaatcgct cagtatcatt aggtgatata gatgaagatg aagccccaca
     3541 aaaaataaga gagataataa gagatgaata tcttagagat acatcagttc ttatccttct
     3601 tgttggaact gaaacaaaaa acagaaaaca cgtagattgg gaactttact ctagcatgag
     3661 agatagtacc attaacaaaa aatcaggaat tttagtcgtc aatctcccgt caacaaatac
     3721 tacgtacatt cgttcaacac atggaactaa tgaaaaatca gaagttcatc ctaccgtaac
     3781 tgaatggttt tctattgata agcgctcaac ttatgaggaa aggttcccat atatgccagc
     3841 aagaataata gacaacttca tgaagagcgg gtcttatata tcagtggtca actggaatca
     3901 aatagaaaac aacccagaaa cactcagaaa attaatagac atgacttata acgatagaga
     3961 aaaagcaacg tatgatatgt cgagagaaat gagaatgcat aattcatagt actatccaag
     4021 gagtagcctt ggctactcct ttaatttacc agttccactt atctgtattg attccaaact
     4081 taggcttcac atcattaaca aagaaccggt taacatcttt aaaacctttt aaaatacgct
     4141 cataatcata atactgcatt tgtattttat ttatgctggt tgcatgaaat ctcatgacat
     4201 caccgctaac cctatcaaca acctcatctt tgtctgatat gtacttatac caacgcttgt
     4261 agttgttctg gtagttagtt agaaatctat ttaacaccat gagcatctca ttgctgaaat
     4321 cataaatctc acgatctttt ttagggttta gtattttcat ttcatcacga ataaattggt
     4381 aagtctgata atatgatgaa ataacctcat caaaggcatc aacgtttttt cttaatttga
     4441 aaattgctct ttttgaatct aggtgatttt ttacactctt tttgtaaagt gtagcactct
     4501 cattgaaaag tatattcact ccaccaaggc taagtttctc aaccttaacc gaatactttt
     4561 taagtaagta gagggatacc atcagccaaa taaaaacaga tatcaaaaga atagaaaggg
     4621 cgccagttac aagaggtcta ttcttactaa aaactgtgta aacagacgga atgtaattag
     4681 atatatcaac agtacacatc acggcaataa atgctaaaaa aactatagtc caaagagtta
     4741 cgaaggtttt catcttattt caccattttt cttgatataa aaatacttcc gcctgcttat
     4801 tcccatagct acccaaggtt ttatttcctc taggcttcct ttcctccttg actgtcctga
     4861 ctttttccct ctctttgatt gaatttcaga ggaatgagta atatcaacaa atttactgaa
     4921 ttcttcaggt gaaaatttat ttgatgtcca tttagcaatg ctcttagcta tccctcttac
     4981 ctcattttca tccagaggcc gtgcaaattg
//
