LOCUS       NZ_CP125179             3511 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 160 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP125179
VERSION     NZ_CP125179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790394
            Assembly: GCF_037477795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 67x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477795.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,813
            CDSs (total)                      :: 5,687
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 88 of 141
            Pseudo Genes (internal stop)      :: 22 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="160"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            564..905
                     /locus_tag="P4G19_RS29045"
                     /old_locus_tag="P4G19_29000"
     CDS             564..905
                     /locus_tag="P4G19_RS29045"
                     /old_locus_tag="P4G19_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1114..>1542
                     /locus_tag="P4G19_RS29050"
                     /old_locus_tag="P4G19_29005"
                     /pseudo=""
     CDS             1114..>1542
                     /locus_tag="P4G19_RS29050"
                     /old_locus_tag="P4G19_29005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1583..2080
                     /locus_tag="P4G19_RS29055"
                     /old_locus_tag="P4G19_29010"
     CDS             1583..2080
                     /locus_tag="P4G19_RS29055"
                     /old_locus_tag="P4G19_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            2087..2302
                     /locus_tag="P4G19_RS29060"
                     /old_locus_tag="P4G19_29015"
     CDS             2087..2302
                     /locus_tag="P4G19_RS29060"
                     /old_locus_tag="P4G19_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2697..2879
                     /locus_tag="P4G19_RS29065"
                     /old_locus_tag="P4G19_29020"
     CDS             2697..2879
                     /locus_tag="P4G19_RS29065"
                     /old_locus_tag="P4G19_29020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          306..479
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(4..382)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.099"
                     /coverage="92.0"
                     /evalue="1.9999999999999997e-142"
     Typing          complement(3123..3511)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.13"
                     /coverage="100.0"
                     /evalue="1.44e-93"
     Typing          896..2418
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
       61 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      121 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
      181 acggcttcgc cgcggcccta tcgcttccct gttaagtatc ttcctggcat cttccaggaa
      241 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
      301 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcgccggtgc
      361 cgcctttttt ctcctgccac atgaagcact tcactgacat cctcatccgt gccaacatag
      421 taagccagta tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc
      481 cactgacgcg cctgcggctt gtccaacccc gcgccgaccg ggaaaggctt tcccgtccgt
      541 cacggcgtta tcaggaggcg attctggcag ataaacgcaa caagatgctc acgatgtggg
      601 tgacggaaga cgaacaccgg cggttgcttg agcggtgcga cggaaaacaa ctggcggcgt
      661 ggatgcgcca gacctgcctt gatgagaaac cgacacgcgc cggcaggctt ccctcgattt
      721 cgcccgcgct gctgcgtcag cttgccggca tgggcaataa cctgaaccag atcgcccgga
      781 aagtgaacgc cggtggtgcc ggtcatgacc gggtgcagat cgtggcagcg ctgatggcga
      841 tcgatgccgg tctggaacgg ttgcggcatg ccgtactgga aaaagggccg gacgatgatt
      901 gttaaattcc atccccgggg tcgcggcggg ggcgccggtc ctgtggatta tcttctgggt
      961 aaagaccggc agcgtgaagg tgccagcgtc ctgcagggga agccggaaga agtccgggag
     1021 ctgattgatg cgtcgccgta tgtgaaaaaa tatacctccg gggtgctgtc attcgcggag
     1081 gcggacctgc cgccgggtca gcgtgaaaaa ctgatggcca gctttgagcg ggttctgatg
     1141 cccggactcg ataaagacca gtacagcatt ctgtgggtgg aacacgcaga caagggtcgt
     1201 cttgagctga attttctgat ccccaatacc gaattactga cggggaaacg cctccagccg
     1261 tattacgatc gtgccgaccg gccgcgcatc gatgcgtggc agacagtggt caacggcagg
     1321 ctggggctac atgatccgaa cgacccggag aaccggcggg cgctggtcac gccttcagga
     1381 ctgccagaaa cgaagcagga agccgcagag acgattacac ggggtttgct gatgttagcc
     1441 tcatcaggag agctgaaaac gcgtgatgac gtcacagggg cgctcacagc ggccggtttt
     1501 gaggtggtca gaaccacaaa aaacagtatc agcattgccg acccggacgg ggggcgaaat
     1561 atccgactga ggggagccat ttatgagcag tcttttaacg gcctcgaggg acttcgagga
     1621 gaaatcgaaa gcacagcagc agcttaccga agagaggctg aaagccgcat tcagcaagca
     1681 cgagaacgtt gtcaaatcgg aactgaacgc aagcgcgaag agaatcaacg acgccatcag
     1741 cgcccacgaa caggggatga acgcggcgat gcagtcgaac cggctgagcg tgctgcgaat
     1801 ggtcgggcgg acgtggctga ccatcaccat ggtgtcaggg ctgctgttcg ccagcctgag
     1861 cggggtgctg tggtaccagg ggaaccggat agcggcgaac ctggcggaaa tcaggcagca
     1921 gagagatacg ctgtcgaaac tgcacatgca gacgtggggc gtgacctatc tggagaaccg
     1981 caacggacgg tttctggtgc tgccggaggg gatgaaagcc gaaacgggcc ggacggtgga
     2041 caacaagacg cggaacgcgg tgaagctggt acgggagtag agcagaatga cggaactgga
     2101 aaaacaattg ctgagcgcgt tagagcagct acagcaggac tactcgagaa ggctggacga
     2161 gtgggagagc gccttcgagg aatggcggaa gatgtctggt cttatgcaac gggagaacgc
     2221 ggcgctgagc gagcgcgtgt cgggcttgag caggcaggtg cagagtttga gcgagcagct
     2281 gcgccggtta tcgacaggct gaatgagatt gagcaacagc gatacgggca aacaacggaa
     2341 aaggaaccag agaagacagc agatctggta ccggaacggc aacgcacatt atctcatggt
     2401 gacagggagt atggagactg ataaacgccc gtctcaggtg gcctgagcgt gacggggatc
     2461 atggccattt tctgcgattt cagcacacag agagtctttt ttaaccgaaa tcaccggcta
     2521 tgcagtgatg tgaataagtg atgcatatgc gtaaaataaa tcaggtgcgt ttctggtggt
     2581 tttcggtgta cttgccacca ccttttgccc gtcagccatc agaaacgccc tgagtgcctc
     2641 cggcgcggtg cgggtaagag atgttatgtt taatgttgat aacaggagta agagtgatgc
     2701 ttacatttat tggtgtagtg gtagtcgccg ttattgcatt taaattattt atgctttttt
     2761 ttagttcatc cgaatctgcc gtcgaacgtg ctacccgtaa atatatggag aaccctacga
     2821 gtgctaacta taaaataatg gtggctacac gcatgagaaa tgagaagaaa agagggtgat
     2881 atatgtttga gtttattggt tttttatttt tacttttggt gtgttatgtc tttatacaaa
     2941 tggttgctgt cagggtgttt cctgaatatg gagtacggaa agcggaggaa cgataccgtc
     3001 gaaatccatc agcagaaaat gaggatctgt tatggagagc tagaagtcgt ataactaaac
     3061 gcaagtaatg tttcctaagt gtgttgatag gcaaacatgg tgggagttac tactctcgga
     3121 gttaatgatc ttcttgtgat cctgtcgttt cagtcgtaat ctcttgctct gaaaacgaaa
     3181 aaaccacctg gggaggtggt ttgatcgaag gttaagtctg ttggggaact gcttaaccag
     3241 gtaactgggt atacgagacc gcagatacca aaactgttct ttcagtgtag ccgcagtcag
     3301 gcccatactt caagacctct gaatctctct ctcgtatatc ccagttacca gtggctgctg
     3361 ccagtggcgc tttgtcgtgc tttctcgggt tggactcaag atgatagtta ccgggtaagg
     3421 cgcagcagtc ggactgaacg gggggtttgt gcatacagtc cagcttggag cgaactgcct
     3481 acccggaact gagtgtcagg cgtggaatga g
//
