LOCUS       NZ_CP125178             4052 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 160 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP125178
VERSION     NZ_CP125178.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790394
            Assembly: GCF_037477795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 67x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477795.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,813
            CDSs (total)                      :: 5,687
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 88 of 141
            Pseudo Genes (internal stop)      :: 22 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="160"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            627..968
                     /locus_tag="P4G19_RS29020"
                     /old_locus_tag="P4G19_28975"
     CDS             627..968
                     /locus_tag="P4G19_RS29020"
                     /old_locus_tag="P4G19_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1177..>1608
                     /locus_tag="P4G19_RS29025"
                     /old_locus_tag="P4G19_28980"
                     /pseudo=""
     CDS             1177..>1608
                     /locus_tag="P4G19_RS29025"
                     /old_locus_tag="P4G19_28980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1646..2131
                     /locus_tag="P4G19_RS29030"
                     /old_locus_tag="P4G19_28985"
     CDS             1646..2131
                     /locus_tag="P4G19_RS29030"
                     /old_locus_tag="P4G19_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_180160425.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            2135..2347
                     /locus_tag="P4G19_RS29035"
                     /old_locus_tag="P4G19_28990"
     CDS             2135..2347
                     /locus_tag="P4G19_RS29035"
                     /old_locus_tag="P4G19_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2855..3313)
                     /locus_tag="P4G19_RS29040"
                     /old_locus_tag="P4G19_28995"
     CDS             complement(2855..3313)
                     /locus_tag="P4G19_RS29040"
                     /old_locus_tag="P4G19_28995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          361..540
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(2..378)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.976"
                     /coverage="92.0"
                     /evalue="1.74e-127"
     Typing          3969..4052
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.88e-36"
     Typing          959..2463
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.024"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
       61 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaatggca ggaacaggag
      121 agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct gtcgggtttc
      181 gccaccactg atttgagcgt cagattttat gatactcgtc aggggggcgg agcctatgga
      241 aaaacggcaa cgccgcatcc tcttcccggc ttctcctgat ttgagcgctg cttgcgtatc
      301 ccgtcatctg tgagctggta acgcccgccg cagtctcacg gcaggagcgc agcgactgag
      361 tgagcgagga ggcgtcattt cacctttacc agcatgttgc acttacgccc gtcagccaca
      421 ttttcccctt cccacatgaa gcactttcat acattaccac tccgccgtaa catatttagc
      481 cagtatacac tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa
      541 aagccactga cgcgcctgcg gcttgtccaa caccgtgccg accgggaaac atttcccgcc
      601 cgtcccggtg ttatcaggag gctactctgg cagacaaacg caacaagatg ctcacgatgt
      661 gggtgacgga agacgaacac cggcggctgc tcgagcggtg cgacggcagg cagctggcgg
      721 cgtggatgcg tcagtcctgc ctcgatgaga agccggcgcg tgcctgcaga ctgccgtcaa
      781 tatcgccggc gctgctgcgc cagcttgccg gtatgggtaa caacctgaac cagatcgccc
      841 ggaaagtgaa tgccgttggt gccggtcatg atcgggtgca gattgtggcc gccctgatgg
      901 caatagacgc gggactggag cggctgcggc atgcggtgct ggaaaaaggg ggggatgatg
      961 atcgttaaat ttcatccccg ggggcgcggt ggtggtgccg gtccggtgga ttacctgctg
     1021 ggaaaagacc ggcagcgcga gggggcgacc gtgctgcagg gaaaaccgga ggaagtccgg
     1081 gagcttatcg atgcctcgcc gtatgcgaaa aagtacacct ccggcgtgct gtcgttcgcg
     1141 gaggctgaac tgctgcccgg gcagcgggag cagatcatgg ccagctttga gcgggttctg
     1201 atgcccgggc tcgataaaga ccagtacagc attctgtggg tggagcacac cgacaagggg
     1261 cggctggagc tgaatttcct gatccccaac actgaactgc tgaccgggaa acgcctgcag
     1321 ccgtattacg atcgcgccga ccggccgcgc atcgatgcct ggcagaccgt ggtgaacggc
     1381 agactgggac tgcatgatcc aaacgccccg gagaaccggc ggttactggt tacgccgtcc
     1441 gcactgccgg aaacgaagct ggaagccgct caggcgatta cacgtgggtt gctggcctta
     1501 gcctcatccg gggagctcaa aactcgcgag gacgtcacag gtgcgctcac agcggcaggt
     1561 tttgaggtgg tccgcactac aaaaaacagt atcagcattg ccgacccgga cggggggcga
     1621 aacatccgac tgaagggagc catttatgag cagtctttta gcgctggcga aggacttgga
     1681 gcagcaatcg aaagcgcagc agcagagtac cggcgagatg ctgaaagccg cattcagcga
     1741 gcacgagaaa tctgtaaaag cggaactgag cgcaagcgcg aagagaatca gcgacgccat
     1801 cagcgcccac gagcagggta tgaccgcggc gatgcagtcg aaccgcctga gcgtgctgcg
     1861 tatggtcggc cggacgtggc tgaccatcac cctggtctcg gtactgctga tcgccatgag
     1921 cgggagcatt ctgtggtggc aggggcagca gatcaccggg aattaccaga ctatccgggc
     1981 gcaggagcgg acgcaggcca tgttgtcgga gaaaaaccac ggcgttcagc tgtcgctctg
     2041 cggggagaag aaactgtcct gcgtcaaagt gaacgcgaaa gcgggagctt acggggagga
     2101 agggaactgg atggtactgg agaggaaata gcccatgacg gaactggaaa aacagttgct
     2161 gaccgcatta gagcagctac atcaggacta ctcgcaaagg ctggacgagt gggagagcgc
     2221 cttcgcggaa tggcagaaga tgtctggtct tatgcaacgg gagaacgcgg cgctgaacga
     2281 gcgcgtcacg cgcttgagtc agcaggtggc gaacttgagc aggcagttac agcgtttgag
     2341 ccagtagtgc agcggcatga aatggcggtg gccgccgaac gggcatacga gcagcgacag
     2401 cacgaaaaag agctggcgga agcccgttcc cgtcagcgga gctataacgg tccgacgctg
     2461 ggctgacagg cttcaccagt gacaaacccc acgaagaagg gctcataaat ttcccgctaa
     2521 atacggtcgt gatgaaaaca tcacggcgta tacaacggag ggattatgag actgtttaac
     2581 gtgattgaga cggtgctgaa ctgcctgtac atggcgctgg agatctggga ctggctggag
     2641 aagcattttc ctgtttttaa ggcgttcatc cagattctgg cctgaaatca gaccgtatgc
     2701 atctggctgc atgaccatgc agccgggtaa aatttatctg tcacgaacgg ggcggtgtat
     2761 cgggtggttt gttgccggtt ttacctgact gccgccccgt ttgtgacgaa catgtccgga
     2821 gcggtgcggg caagagatgt tatggtaaat catattagtt ctgaatgatt cgggctccac
     2881 cagtcattac tatttgagca ccacctgaca tttttatact tgtaccctgg ctagtcattg
     2941 tcgtatcacc ttgtatgatc gtttttccag tagcatgttg acctccacca gtgactaaaa
     3001 ttgacgcttg tacttgagat ccatttatca aaacagttcc ggcttggtga tcaaatttta
     3061 ttccgctcaa tgcttttaat tcagctccat ctagctgaat ttcaccattg gctgctgcag
     3121 cctgaatggc cccctgcatc tgagaaaata atttatcaac ttcgtcaaaa ggtaatggtg
     3181 tttcaagcag tgaggcttgt tgttcaactt caggatgttt acttgctaac ttccctatga
     3241 tgcctttgat tgcatcagaa gtaagattgc ttaatgcgcc agtgagaacg gcaagtagtg
     3301 tggtttcaat cattatattt ccctcgttag attgctgtga tgcgtattat ttactcttta
     3361 taatctccga ttctgtccat atggttagtt gcactattac gtttataagt gccactttta
     3421 ccatatatat atccaacgct acattgtgga cattttggcg tctcgtcatt atgagtgaaa
     3481 tttatttctc ctccgacaga tagggttttt acgttttccg gataatctaa tgtagccacg
     3541 ccggtccatt cacaattcga gcagacaaca tcgaaagatg ttgggatact cttgcagttg
     3601 atgttgattt gcatatgcat aacctcgatt ttgcacagaa attacagtgt tattagttgc
     3661 ataccgacta taacctgagt ttgtgatgga gtggtgtttt tttgctttga cttgagtaag
     3721 agaaagagat gatcttcttg agataatctc tttctgagcg tagtcttttg ctctgtaaac
     3781 gaaaaaacca ccctggcagg tggtttttcg aaggttagtt aatcctggca gatttactaa
     3841 ccgtggtaac agtcttgtgc gagacatgtc accaaagctg tccttccagt gtagccgcag
     3901 taaggccacc acttcaagaa ctctgaatac atctctcgca catcctgttt accagtggct
     3961 actgccaatg gcgatttgtc gtgccttttc gggttggact caagacgata gttaccgaaa
     4021 agagcgtggt agtcggactg aacgggggga tc
//
