LOCUS       NZ_CP125177             5010 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 160 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP125177
VERSION     NZ_CP125177.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790394
            Assembly: GCF_037477795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 67x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477795.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,813
            CDSs (total)                      :: 5,687
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 52 of 141
            Pseudo Genes (incomplete)         :: 88 of 141
            Pseudo Genes (internal stop)      :: 22 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125177.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="160"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(4964..5010,1..859))
                     /locus_tag="P4G19_RS28985"
                     /old_locus_tag="P4G19_28940"
     CDS             complement(join(4964..5010,1..859))
                     /locus_tag="P4G19_RS28985"
                     /old_locus_tag="P4G19_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            complement(1107..1382)
                     /locus_tag="P4G19_RS28990"
                     /old_locus_tag="P4G19_28945"
     CDS             complement(1107..1382)
                     /locus_tag="P4G19_RS28990"
                     /old_locus_tag="P4G19_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(1363..1608)
                     /locus_tag="P4G19_RS28995"
                     /old_locus_tag="P4G19_28950"
     CDS             complement(1363..1608)
                     /locus_tag="P4G19_RS28995"
                     /old_locus_tag="P4G19_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            1845..2006
                     /locus_tag="P4G19_RS29000"
                     /old_locus_tag="P4G19_28955"
     CDS             1845..2006
                     /locus_tag="P4G19_RS29000"
                     /old_locus_tag="P4G19_28955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            complement(2806..3207)
                     /locus_tag="P4G19_RS29005"
                     /old_locus_tag="P4G19_28960"
     CDS             complement(2806..3207)
                     /locus_tag="P4G19_RS29005"
                     /old_locus_tag="P4G19_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3609..4223
                     /locus_tag="P4G19_RS29010"
                     /old_locus_tag="P4G19_28965"
     CDS             3609..4223
                     /locus_tag="P4G19_RS29010"
                     /old_locus_tag="P4G19_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(4260..4967)
                     /locus_tag="P4G19_RS29015"
                     /old_locus_tag="P4G19_28970"
     CDS             complement(4260..4967)
                     /locus_tag="P4G19_RS29015"
                     /old_locus_tag="P4G19_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
ORIGIN
        1 ttattcccat agctacccaa ggttttattt cctctaggct tcctttcctc cttgactgtc
       61 ctgacttttt ccctctcttt gattgaattt cagaggaatg agtaatatca acaaatttac
      121 tgaattcttc aggtgaaaat ttatttgatg tccatttagc aatgctctta gctatccctc
      181 ttacctcatt ttcatccaga ggccgtgcaa attgcaggtt gtatgcgcaa gcgcgttcgt
      241 agcaggcttg cagccactgc tcatactcag gccagccctg acggatggcg cgatatgccc
      301 acttacgggt tttgtcgaac aacgtgcagt tacggcctaa gccgtagtcg gcaacgattt
      361 ccttgtcatt ccctgtgttc agatcgagaa agtcggccag ccagtccaga gtgtagagct
      421 tcggctgcca cacggcgatt ttccagtggt tgtggttcgg atttttgcag atgagccccg
      481 aatatccagc gtcagcctct aattttttac gtagcgcatt ctccacggag gcggcgtagc
      541 gtaacggttt tattttgccg tccggcgcgg tgcgtatcga gttttccagc gcgtacagca
      601 ggtgcgcgtg tccgttttcg gggttagtta tggtcagcgt cggtgcaggc gcgttgcggt
      661 cgctccagtc gatggaggaa ccggcgcggt caacgtcaaa tcccagccag aacatggcgt
      721 gaggctgatt gaactggata tatttcgcca gaatagcgcg ttccttaccg gcgatacgga
      781 caccaaagtg cagatcgtcc gaaaagtacg gcttgtgtgg taagcgttcg ttgaaaagtt
      841 cgagtgcggc gttatgcaat gtgccctgcg cttatggcgg gctgttgtct ggcacggttc
      901 atgtgctaat atcctttcaa ggtttcgacc tagccctggt tatcatgcga tccgccaaga
      961 tatgacatga tttccggggc tttgtttttt ctgaggctaa cgttacaccg actttgcctt
     1021 tgtgtcgatc tgacagcgac ttaccctaac atccgttgta cgccttgcat gaaaagcccg
     1081 catcaagcgg gctatatcga cttttgctat ttgtcaccat agtgataacg ggctgcgtag
     1141 atgatgattt ctccgttctg gattgaatag accagacggt ggctctgatc aatcctgcgg
     1201 gagtagagtg ccggatcttt gtggtgccgc agccgttcgg gttttcctat acctgtcagc
     1261 ggatcagcct cgatgctgag caacagttgc cggatacgct caaacttctt tttgtcagta
     1321 tcacgccagt agttaaggtc atcagcgcca ttcctagtga acttaatatt ccacttcgac
     1381 aaattcgccg cgctccgcct gtttcattga ctcattgata tgctctgcgt tcgccggatt
     1441 gctgaacaga tagaccgttt cgattagcgc ctcatactct cccgcatcga tcataacaat
     1501 atcaggcgca tcacggcgca tgacacgtac cggttcagca tcatcagtga cgcgcttcat
     1561 cgtctcggca aaatgtttac gggcatcagt aaaattgatt gcatgcatgt tgttaacccc
     1621 catttgttgg ggcttagcat agaacatgta cgcaaataag tacataaaga tatttcccta
     1681 aaaacccaca accaaatcag ctcaaaatgt gaactcaatc gaataaccac aaaaacaaca
     1741 agtcaataca ttgaagcata atttcaagca attacattta tgaagtgcgg cacaatttcg
     1801 taccgctagg agcttagctc ctaaaacgag caaaggaaaa gtaaatggca aaacattggt
     1861 ctgggcaaga atgtccaaaa acaggtactt acggtcaata tcatgatacc gacaactcat
     1921 acgctggttc tgaatatgat cgccatgtga gcaagggtga taagttccct ccgagcaaaa
     1981 acaaccacca cttccaagaa aaataaaata tcttcagaag tctaaactaa aagccctctg
     2041 gggctttttt tgtacctgcc attcacttga tttaccataa catccgttat cagcaccgcc
     2101 tgagatgccc tcagggcgtt tctgacggga aaccggtaaa caaggcaaca aaccacccga
     2161 aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcacaagtg
     2221 catagcgcgg aaattgacac cattcagcca ggggaagtcc tgtttccggc gttcttccca
     2281 tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag
     2341 ccggaaaacc ggttttcagc acgttcagga atcggttcgt ccacggccgg caatcacgtt
     2401 atggcaagtc gccggacgac tcaccacacg attccccggc gactcttcac tagagtcgta
     2461 cccagagtcg ccacgtctaa aaaacaacca aacggggagt cggatctaga gtcgggtcgt
     2521 tattaactca ttgtttttat aggcctgagt cgttaccgac tctatgacga ctcgccgggg
     2581 actctatgac gactcgtcat agagtcggat ctatataaat aaaatttaaa aaacgaaatt
     2641 tcagtaaagt aagctgctga tattaaatac aaatctgcaa aattaaaatt ttgcgtgggg
     2701 tgtgggtatt tttcgtggtg aggagcgttc agagatctga cgattttcgg gaaggttcag
     2761 gaactccccg gaatggccgg agaggaaaac tgaagcatga cggggtcagg attttttcca
     2821 gaatttccac cacggcgccg ggggttcagt taattttgcc tggtgatttt caagaaggag
     2881 catggcctgt ttcaggctgt caatgtgcga atccttcgat aaaatcaata tgttaaggtg
     2941 ttcaatctgc tgtttcagtt gacgaatgac atgttcactc tggtcagcag gcgctgtatc
     3001 acgctgttca ggtacttgtc gagtaaggtg ttcattcttc ggttggtcaa acggtccgaa
     3061 aacacgtaaa agctctgata tatcgaccat ttttgtattt cgttcaccgc tacaagtgga
     3121 cagttcacct gttttgatta acctgtgcag cgttgtccgg ctcttacccg tcagccgtgc
     3181 agcctcacta atagacactc tggtcatcag ttatcatcct ttaatacatt cagtctcaag
     3241 gaaacagggg ccagcttacg acatactggt ccagaagtga tgtgtttttc agaagggcga
     3301 tgaaacacag gagtgaccgt gcaaaatgca ggcaacaaat gactttccgc ttcctcgcgc
     3361 attcgctcac tacgttcggt cataagactg cggcgagcgg tacgtaagaa taaagtgagg
     3421 tgatagctct cttctcaagg cgtttaggga agcaacgagc ccgccctaaa ccagtcatgg
     3481 cgagaccttg ctgaagggac cctatgtgtg ctggctgaga aaaaaagagc tctctaaaag
     3541 agagctgtta tctgatatag gttcaagtgc aaaatagagt gcacacaaac aaaaaaagga
     3601 caataaaaat gggaaagcat aaggtattca ttagctacca ccatgcgaat gatcagtact
     3661 acaaaaacgc acttgaaaaa atgaatgagg aacatgaaat attcgtgaat cgctcagtat
     3721 cattaggtga tatagatgaa gatgaagccc cacaaaaaat aagagagata ataagagatg
     3781 aatatcttag agatacatca gttcttatcc ttcttgttgg aactgaaaca aaaaacagaa
     3841 aacacgtaga ttgggaactt tactctagca tgagagatag taccattaac aaaaaatcag
     3901 gaattttagt cgtcaatctc ccgtcaacaa atactacgta cattcgttca acacatggaa
     3961 ctaatgaaaa atcagaagtt catcctaccg taactgaatg gttttctatt gataagcgct
     4021 caacttatga ggaaaggttc ccatatatgc cagcaagaat aatagacaac ttcatgaaga
     4081 gcgggtctta tatatcagtg gtcaactgga atcaaataga aaacaaccca gaaacactca
     4141 gaaaattaat agacatgact tataacgata gagaaaaagc aacgtatgat atgtcgagag
     4201 aaatgagaat gcataattca tagtactatc caaggagtag ccttggctac tcctttaatt
     4261 taccagttcc acttatctgt attgattcca aacttaggct tcacatcatt aacaaagaac
     4321 cggttaacat ctttaaaacc ttttaaaata cgctcataat cataatactg catttgtatt
     4381 ttatttatgc tggttgcatg aaatctcatg acatcaccgc taaccctatc aacaacctca
     4441 tctttgtctg atatgtactt ataccaacgc ttgtagttgt tctggtagtt agttagaaat
     4501 ctatttaaca ccatgagcat ctcattgctg aaatcataaa tctcacgatc ttttttaggg
     4561 tttagtattt tcatttcatc acgaataaat tggtaagtct gataatatga tgaaataacc
     4621 tcatcaaagg catcaacgtt ttttcttaat ttgaaaattg ctctttttga atctaggtga
     4681 ttttttacac tctttttgta aagtgtagca ctctcattga aaagtatatt cactccacca
     4741 aggctaagtt tctcaacctt aaccgaatac tttttaagta agtagaggga taccatcagc
     4801 caaataaaaa cagatatcaa aagaatagaa agggcgccag ttacaagagg tctattctta
     4861 ctaaaaactg tgtaaacaga cggaatgtaa ttagatatat caacagtaca catcacggca
     4921 ataaatgcta aaaaaactat agtccaaaga gttacgaagg ttttcatctt atttcaccat
     4981 ttttcttgat ataaaaatac ttccgcctgc
//
