LOCUS       NZ_CP125172             8181 bp    DNA     linear   CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 161 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP125172
VERSION     NZ_CP125172.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790395
            Assembly: GCF_037477785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 103x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477785.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:33:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,463
            CDSs (total)                      :: 5,343
            Genes (coding)                    :: 5,169
            CDSs (with protein)               :: 5,169
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 73 of 174
            Pseudo Genes (incomplete)         :: 104 of 174
            Pseudo Genes (internal stop)      :: 32 of 174
            Pseudo Genes (multiple problems)  :: 33 of 174
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="161"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(6..857)
                     /gene="repC"
                     /locus_tag="P4G16_RS27220"
                     /old_locus_tag="P4G16_27215"
     CDS             complement(6..857)
                     /gene="repC"
                     /locus_tag="P4G16_RS27220"
                     /old_locus_tag="P4G16_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(844..1683)
                     /locus_tag="P4G16_RS27225"
                     /old_locus_tag="P4G16_27220"
     CDS             complement(844..1683)
                     /locus_tag="P4G16_RS27225"
                     /old_locus_tag="P4G16_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(1713..1919)
                     /gene="urfF"
                     /locus_tag="P4G16_RS27230"
                     /old_locus_tag="P4G16_27225"
     CDS             complement(1713..1919)
                     /gene="urfF"
                     /locus_tag="P4G16_RS27230"
                     /old_locus_tag="P4G16_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(1921..2133)
                     /locus_tag="P4G16_RS27235"
                     /old_locus_tag="P4G16_27230"
     CDS             complement(1921..2133)
                     /locus_tag="P4G16_RS27235"
                     /old_locus_tag="P4G16_27230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(2194..3165)
                     /locus_tag="P4G16_RS27240"
                     /old_locus_tag="P4G16_27235"
     CDS             complement(2194..3165)
                     /locus_tag="P4G16_RS27240"
                     /old_locus_tag="P4G16_27235"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_010890158.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(3162..3575)
                     /locus_tag="P4G16_RS27245"
                     /old_locus_tag="P4G16_27240"
     CDS             complement(3162..3575)
                     /locus_tag="P4G16_RS27245"
                     /old_locus_tag="P4G16_27240"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(<3682..4323)
                     /locus_tag="P4G16_RS27250"
                     /old_locus_tag="P4G16_27245"
                     /pseudo=""
     CDS             complement(<3682..4323)
                     /locus_tag="P4G16_RS27250"
                     /old_locus_tag="P4G16_27245"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            4523..4807
                     /locus_tag="P4G16_RS27255"
                     /old_locus_tag="P4G16_27250"
     CDS             4523..4807
                     /locus_tag="P4G16_RS27255"
                     /old_locus_tag="P4G16_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5361..>5765
                     /locus_tag="P4G16_RS27260"
                     /old_locus_tag="P4G16_27255"
                     /pseudo=""
     CDS             5361..>5765
                     /locus_tag="P4G16_RS27260"
                     /old_locus_tag="P4G16_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(6107..6355)
                     /locus_tag="P4G16_RS27265"
                     /old_locus_tag="P4G16_27260"
     CDS             complement(6107..6355)
                     /locus_tag="P4G16_RS27265"
                     /old_locus_tag="P4G16_27260"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_319839125.1"
                     /translation="MDSKHFLKILNCHFTSILWTVDAEQDRERIYDYLDERNPIAAIEL
                     DDLIREKVSLLAHNNLMGRTGQKKGTRELKTMSEANP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6351..7130)
                     /locus_tag="P4G16_RS27270"
                     /old_locus_tag="P4G16_27265"
     CDS             complement(6351..7130)
                     /locus_tag="P4G16_RS27270"
                     /old_locus_tag="P4G16_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_094965899.1"
                     /translation="MKLPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTATTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEVLNLLNSIAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(7535..>8181)
                     /locus_tag="P4G16_RS27275"
                     /old_locus_tag="P4G16_27270"
     CDS             complement(7535..>8181)
                     /locus_tag="P4G16_RS27275"
                     /old_locus_tag="P4G16_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_309200587.1"
                     /translation="RGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIAAELMA
                     KLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNASELRG
                     LHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIAQMADA
                     FSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside phosphotransferase family protein"
     ARG             complement(6355..7130)
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.07"
                     /coverage="100.0"
     ARG             complement(7536..8179)
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.53"
                     /coverage="76.98"
     Typing          complement(4413..4569)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.367"
                     /coverage="100.0"
                     /evalue="2.97e-75"
     Typing          complement(845..1666)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(2198..4323)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggggtcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt
       61 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg
      121 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa
      181 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg
      241 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc
      301 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg
      361 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg
      421 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg
      481 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca
      541 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc
      601 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg
      661 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg
      721 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
      781 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg
      841 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
      901 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
      961 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
     1021 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
     1081 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
     1141 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
     1201 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
     1261 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
     1321 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
     1381 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
     1441 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
     1501 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
     1561 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
     1621 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
     1681 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     1741 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     1801 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     1861 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     1921 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     1981 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     2041 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     2101 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     2161 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
     2221 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
     2281 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
     2341 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
     2401 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
     2461 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
     2521 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg
     2581 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
     2641 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
     2701 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
     2761 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
     2821 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
     2881 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
     2941 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
     3001 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
     3061 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
     3121 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcgc
     3181 agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt cgagcaagag caacgatgcg
     3241 atcagcagca ccaccgtagg catcatggaa gccagcatca cggttagcca tagcttccag
     3301 tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc tgctcacctc ggcggctacc
     3361 tcccgcaact ctttggccag ctccacccat gccgcccctg tctggcgctg ggctttcagc
     3421 cactccgccg cctgcgcctc gctggcctgc tgggtctggc tcatgacctg ccgggcttcg
     3481 tcggccagtg tcgccatgct ctgggccagc ggttcgatct gctccgctaa ctcgttgatg
     3541 cctctggatt tcttcactct gtcgattgcg ttcatggtct attgcctccc ggtattcctg
     3601 taagtcgatg atctgggcgt tggcggtgtc gatgttcagg gccacgtctg cccggtcggt
     3661 gcggatgccc cggccttcca tctccaccac gttcggcccc aggtgaacac cgggcaggcg
     3721 ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg gcgtcgtggc cagcccgctc
     3781 taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc tgctcaagcc atgccttggg
     3841 cttgagcgct tcggtcttct gtgccccgcc cttctccggg gtcttgccgt tgtaccgctt
     3901 gaaccactga gcggcgggcc gctcgatgcc gtcattgatc cgctcggaga tcatcaggtg
     3961 gcagtgcggg ttctcgccgc caccggcatg gatggccagc gtatacggca ggcgctcggc
     4021 accggtcagg tgctgggcga actcggacgc cagcgccttc tgctggtcga gggtcagctc
     4081 gaccggcagg gcaaattcga cctccttgaa cagccgccca ttggcgcgtt catacaggtc
     4141 ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc ggcatgtgcc cggattcggc
     4201 gtgcaagact tcatccatgt cgcgggcata cttgccttcg cgctggatgt agtcggcctt
     4261 ggccctggcc gattggccgc ccgacctgct gccggttttc gccgtaaggt gataaatcgc
     4321 catgctgcct cgctgttgct tttgcttttc ggctccatgc aatggccctc ggagagcgca
     4381 ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga aaccggtaag tgcgccctcc
     4441 ccctacaaag tagggtcggg attgccgccg ctgtgcctcc atgatagcct acgagatagc
     4501 acattaacaa tggggtgtca agatggttaa ggggagcaac aaggcggcgg atcggctggc
     4561 caagctcgaa gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga
     4621 acagcagcaa gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt
     4681 ggccaaggtg aacagcagcg agtggccgga ggatcggctc atggcggcaa tggatgcgta
     4741 ccttgaacgc gaccacgacc gcgccttgtt cggtctgccg ccacgccaga aggatgagcc
     4801 gggctgaatg atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg
     4861 gtagggggaa aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg
     4921 tgtggggttt agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg
     4981 tagcctagcg cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg
     5041 cagcagcgcc ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat
     5101 ggatggattt ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg
     5161 cgtgacagtt attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca
     5221 ggggttcgtg acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc
     5281 aggcatttcg gctgagggta aaagaacttt ccgctaagcg atagactgta tgtaaacaca
     5341 gtattgcaag gacgcggaac atgcctcatg tggcggccag gacggccagc cgggatcggg
     5401 atactggtcg ttaccagagc caccgacccg agcaaaccct tctctatcag atcgttgacg
     5461 agtattaccc ggcattcgct gcgcttatgg cagagcaggg aaaggaattg ccgggctatg
     5521 tgcaacggga atttgaagaa tttctccaat gcgggcggct ggagcatggc tttctacggg
     5581 ttcgctgcga gtcttgccac gccgagcacc tggtcgcttt cagctgtaag cgtcacggtt
     5641 tctgcccgag ctgtggggcg cggcggatgg ccgaaagtgc cgccttgctg gttgatgaag
     5701 tactgcctga acaacccatg cgtcagtggg tgttgagctt cccgtttcag ctgcgtttcc
     5761 tgtttggggt cgtttgcggg aaggggcgga atcctacgct aaggctttgg ccagcgatat
     5821 tctccggtga gattgatgtg ttcccagggg ataggagaag tcgcttgata tagttgtgcg
     5881 tcaatctgtc atagttaccc ccgtcaaagc cacgtagagc aaggcatagc gaggagctaa
     5941 aaatatttaa aaatattaaa aaatattaaa aaaagaaaaa atttaaaaaa acctgtggat
     6001 aactgcccta tgtactcgct ttgctcgtac tttttgacct cgctttgctc ggtcgtgggg
     6061 gcagttattt tctaatcctg tttgtgactt caaaaagcaa aagcgactat ggattcgctt
     6121 cgctcatagt ttttaactct cttgttcctt ttttctgtcc agttctcccc atgagattat
     6181 tatgagcaag caaactaact ttttcccgaa ttaaatcatc caattcaatt gcagcaattg
     6241 gatttctttc atcaagataa tcataaattc tttcacgatc ttgttctgca tctacggtcc
     6301 aaagaatcga cgtaaaatga caattaagta tttttagaaa atgcttggaa tcaattcaat
     6361 tcatcaagtt ttaaaaaata attccttttg tcaggtctat cattttttaa atgctttaaa
     6421 aatattttag cagtttcctc cgatgcatcc tctcttaggc aacgttcaac aaaggatata
     6481 tctacaaact catctgctaa cccagcacgg ccaagatcta aaaaataaat ttcattgaat
     6541 ttatctataa aaatattact atccgtgata tcgccatgag aaaaaaccaa tctttcttca
     6601 acacgagtct catttaactc attccataga cttaggtaag ttttatggtc tccccataat
     6661 tcagcgtcaa aatcatcttg atctatatca tcaaggagtt ggttatcaat aaaaaatttt
     6721 gactctttta accgatgatc aatgtttgaa ataaatggac aatcaataat agcaattgaa
     6781 tttaacagat tgagtacctc cttatagata gcaagcaatt cttggtctgt taaaaaaagc
     6841 gctgaaattg gttttgcatt gatcgcttta gtgatcatga attcaaactg ctcatcctga
     6901 aaagtcatga tgagttcagg cacctttaac ttctcagaga gccaactcaa cattttcgct
     6961 tcacgagaaa cactgtatgt ggtcgctgta tataaagtgc tagatcgctt aagaaaaaaa
     7021 gtttcattat ttcgattaaa agaataaaca tccgatggcg actgaccaat tttatttggc
     7081 tctaaaacgc tgtttccaat aaattgttga ataatattgg gcaatttcat caagctttcc
     7141 ttttattcag tattaaaaac cccgcaaatg cgaggcctag taaatagatg atcttaattt
     7201 ggttcactgt agcaaaaata tggcttattc cctagaagaa cttgcaaaat tattgtccca
     7261 gccgacacag ctgtagctcc tcttaaaaaa gaggcttttt tttaatcaaa cccgagttgc
     7321 gctatgattt tagggatcaa tcatcttgtt tctccatatc ttaaacaagc tgatgcgttg
     7381 acctgtgtca aatattcaat cattaagcac ttaatcaatt gccttgtcgg ggtcgtttgc
     7441 gggagagggc gaaatcctac gctaaggctt tggccaacga tattctccgg taagattgat
     7501 gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct
     7561 tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg
     7621 cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg
     7681 cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc
     7741 tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga
     7801 cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc
     7861 catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt
     7921 ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg
     7981 caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca
     8041 gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga
     8101 tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga
     8161 gcaaccggac tgctcccctc c
//
