LOCUS       NZ_CP125150             4167 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 180 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP125150
VERSION     NZ_CP125150.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790398
            Assembly: GCF_037477765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477765.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,513
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 38 of 109
            Pseudo Genes (incomplete)         :: 60 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 10 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125150.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="180"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(4006..4167,1..81))
                     /locus_tag="P4G17_RS27500"
                     /old_locus_tag="P4G17_27480"
     CDS             complement(join(4006..4167,1..81))
                     /locus_tag="P4G17_RS27500"
                     /old_locus_tag="P4G17_27480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(447..1079)
                     /locus_tag="P4G17_RS27505"
                     /old_locus_tag="P4G17_27485"
     CDS             complement(447..1079)
                     /locus_tag="P4G17_RS27505"
                     /old_locus_tag="P4G17_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1079..2194)
                     /locus_tag="P4G17_RS27510"
                     /old_locus_tag="P4G17_27490"
     CDS             complement(1079..2194)
                     /locus_tag="P4G17_RS27510"
                     /old_locus_tag="P4G17_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2539..2652)
                     /locus_tag="P4G17_RS27515"
                     /old_locus_tag="P4G17_27495"
                     /pseudo=""
     CDS             complement(2539..2652)
                     /locus_tag="P4G17_RS27515"
                     /old_locus_tag="P4G17_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3068..3259
                     /locus_tag="P4G17_RS27520"
                     /old_locus_tag="P4G17_27500"
     CDS             3068..3259
                     /locus_tag="P4G17_RS27520"
                     /old_locus_tag="P4G17_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3259..3660
                     /locus_tag="P4G17_RS27525"
                     /old_locus_tag="P4G17_27505"
     CDS             3259..3660
                     /locus_tag="P4G17_RS27525"
                     /old_locus_tag="P4G17_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2540..2947)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 ccgttcaccg ttttcggagg agaagaacgc cggaaacagg acttcccctg gctgaatggt
       61 gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg
      121 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa
      181 cgtcctgagt ccgttttagc ggtgcgtaca attatgattt tatgttaaat tgttttaagt
      241 gaatagagag aatataactt aagggagcat cttgtgctgg gtcttttaca ttagatctgc
      301 acaatcattg ttttggcatc tggtgatgtc tttaggacgt ttgagattgc tgataactaa
      361 agtgtatgat gctttctata cgttatccgg cgtttaataa caccctggtg ataagccagc
      421 ccgtgaaggg ctggcttgat ttggatttat atttttgaaa taaaatcttt tatttcatca
      481 aagcaattgt ctgctgtgtt tattattcca tttttcatgg atattcctat tcttgagtcg
      541 gattctctat tgcagctaag atagacttgt accttagctt ttttttcgtt tgttggataa
      601 gtgtactctt tgtcactcgc tgagatgtca tctttattat ttttaatttt ttcgaaaaat
      661 tctattatgt attcatataa cgctcgagtg cttgcgtgtt caattatcag atcttcaatg
      721 gcaccttgct tgtcttttcc tggcattata aagaaagaaa tttttgtatt gccattcata
      781 gctatctcgt tatgcgagct gggggaggag atttgtagtt ctcgcaatgt ggcaattatt
      841 gatctttctg tgtcagaaaa tgatgtgtca gcatctaata taataaaaat actttctttt
      901 tcatcgaaag aatcaatgtt tttgaatgct ctaagtgctt gttttaattt tgattttcca
      961 tttatttttt cgacaaaaac atcggtaatg ccttgtattt caagtaggtt gcatagaaaa
     1021 tgaaaatcat catcaccctc aacgaaaatg atctttttgc tattatttat gtctttcatt
     1081 atcttacctc ccagtcgttc tcaactgagt aggagaaatc atccatattg aattgtacgg
     1141 ctgttggttt tagggagttc ttttctcttc ctagtctaat ataagcgata tcttcatttt
     1201 tgctatctat ttcgtttatt gctcgtatca catcttgact atgagttgtt gcaaatattt
     1261 gattgttgtt ttgttttgca gatgaaatta atgattttat tatgtcttta attactgagt
     1321 aatgtattcc gttctcgatt tcatcaataa gaataattga gttttcctgt accagcactg
     1381 agctgataaa agataatgct cgactgatac cctcgccaag cattgatatt tcatttagct
     1441 cactaaatcc aatgtctaaa tatatttcct tattgttacc tatcgcagat attgctatat
     1501 ctacaattct gtcatctatt ttttttaagt tttctagtat gtcttttttc cttttttgtg
     1561 ttagtaggct agatacattt gtaattgttg cctctttgtt tatctttctg gatgttgtta
     1621 ttattgttgc tgtcttaaat ttataattaa tcccatccct ttcgtttttt atttcggatt
     1681 ttatttctac gccatcttct ttcaattgca tagagaaaag cttattggaa accctgtttt
     1741 tcgcattggg tgtctcggtc acatttattt ttaaagagtt tactgtggcc tgagaaccga
     1801 tagtttgacg aggtatgttt tggttagata gtacggtgtc aacgtttaga gacaaacttg
     1861 attctaatct ctgatttgtt tctgtttcat atgtttgtgt tacttcagag cgttcatctc
     1921 gaatgactat ggacatcttt tgtgatgtgt ccatatcatg aaagtaacta acccaaaatg
     1981 gcttgttttt gtttatttct gccatttctt ttctgaatgc ttgaatgttt aacaaaacag
     2041 ccgggttttt tacatcataa aaacaaaaaa tagcatccag tatagatgtt ttcccgtagt
     2101 tgtttttccc agctatgata tttaaagtct ttaattgctt tagatatagc tcattgaagc
     2161 atttgaagtt ctttaaactt atttccttta tcatgatagc ctctgttcgt gtttttgtta
     2221 tatgccatac gatttgttag taccttaata aaatgtacag ctttcattgt gattagctag
     2281 actttatgtc aaagattaac tttgattgct tgtgttttaa tcgatttttt ttactttctt
     2341 cttgagatta tctggcagca gccgtaagct tccggctgtg aacgaaaaaa accgccttgg
     2401 cgggcggttt tttcgaaagg ttcagtagag ttggcgctct atgaaccgag gtaactggct
     2461 taaagggagc gcagtcacca aaactgttcg tttagtttag ctttaactgg cgtataactt
     2521 caagactaac tccctaaatc agttaccagt ggctgctgcc agtggcgctt tgtcgtgtct
     2581 ttccgggttg gactcaagac gatagttacc ggattaggcg cagcagtcgg actgaacggg
     2641 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     2701 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     2761 caggagagcg cacgagggag ccatcagggg gaaacgcctg gtatctttat agtcctgtcg
     2821 ggtttcgcca ccactgattt gagcgtcaga ttccgtgatg ctcgtcaggg gggcggagcc
     2881 tatggaaaaa cggccgctgt gcggcctttc cttctttcct ccatgcgatt actcggtttt
     2941 ctcgagcgtt tctacgcatg ctcctgccgc tcgccgcagc cgaacggcag gagcgtagcg
     3001 actgagtgag cgaggaagcg gaaaagagga gatccctaca ctgaccactg acgctctgag
     3061 gcctcttatg acgaaacaac agaaaacagc gctgaacatg gctaaattca tccagaatca
     3121 gtctctgctc ctgctggaga agcttaacga gcttgatctc gatgtcgagg ccgacctttg
     3181 tgagaaactc cacgatgatg cggagcatct tttccgtaca ctgtcctcga gactggatgc
     3241 attacaggat ggtaactgat ggccagagtg tctattagtg aggctgcccg actgacgggt
     3301 aagagtagaa caacactgca caggttaatt aaagcaggtg acctgtccac ctgtagcggt
     3361 gagcgcaatg caaaaatggt agatacttca gagcttttac gtgtctttgg cccctttgag
     3421 caaccgaagg gtgaacacca ttctggacag gtctctggac agcgtgatac agcctctgct
     3481 gaccagagtg aacaggtgat ccgtcatctg aaacaggaga ttgaacacct gaacacctta
     3541 cttttgtcca aggattcgca cattgacagc ctgaaacagg ccatgctcct tcttgaaaat
     3601 caccaggcaa aattaactga atccccggcg ccatggtgga aattctggaa aaaatcctga
     3661 ccccgtcatg cttcagtttt cctctccggc catcccaggg agttcctgaa ccttcccaaa
     3721 aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac ccacacccca cgcaaaatta
     3781 aaattttgca gattccttat ttacaacaat gggttaatga aaaataaatg tgcggtgatg
     3841 tgttacaaag tgcagatcca agtcgtggac gactctatgg cgaatcgctg acgaattgcc
     3901 cacgattcat atgtgaatca cgctttatta tcatattgtt atgatgaatc gactcttgat
     3961 tcaattcatc actttgggtg ttttttgctc atggtgactc ttgattcaat tcacgattcg
     4021 aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgccggcc
     4081 gtcgacgaac ccgattcctg aaggtgctga aaaccggttt tccggctgcg ccggcaacgc
     4141 ttcacggcca cgcaataaat tgcgtcc
//
