LOCUS       NZ_CP125149             5010 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 180 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP125149
VERSION     NZ_CP125149.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790398
            Assembly: GCF_037477765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477765.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,513
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 38 of 109
            Pseudo Genes (incomplete)         :: 60 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 10 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125149.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="180"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(354..515)
                     /locus_tag="P4G17_RS27465"
                     /old_locus_tag="P4G17_27445"
     CDS             complement(354..515)
                     /locus_tag="P4G17_RS27465"
                     /old_locus_tag="P4G17_27445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            752..997
                     /locus_tag="P4G17_RS27470"
                     /old_locus_tag="P4G17_27450"
     CDS             752..997
                     /locus_tag="P4G17_RS27470"
                     /old_locus_tag="P4G17_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            978..1253
                     /locus_tag="P4G17_RS27475"
                     /old_locus_tag="P4G17_27455"
     CDS             978..1253
                     /locus_tag="P4G17_RS27475"
                     /old_locus_tag="P4G17_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            1501..2406
                     /locus_tag="P4G17_RS27480"
                     /old_locus_tag="P4G17_27460"
     CDS             1501..2406
                     /locus_tag="P4G17_RS27480"
                     /old_locus_tag="P4G17_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            2403..3110
                     /locus_tag="P4G17_RS27485"
                     /old_locus_tag="P4G17_27465"
     CDS             2403..3110
                     /locus_tag="P4G17_RS27485"
                     /old_locus_tag="P4G17_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(3147..3761)
                     /locus_tag="P4G17_RS27490"
                     /old_locus_tag="P4G17_27470"
     CDS             complement(3147..3761)
                     /locus_tag="P4G17_RS27490"
                     /old_locus_tag="P4G17_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            4163..4564
                     /locus_tag="P4G17_RS27495"
                     /old_locus_tag="P4G17_27475"
     CDS             4163..4564
                     /locus_tag="P4G17_RS27495"
                     /old_locus_tag="P4G17_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ctgaaaaccg gttttccggc tgcgccggca acgcttcacg gccacgcaat aaattgcgtc
       61 cccgttcacc gttttcggat gggaagaacg ccggaaacag gacttcccct ggctgaatgg
      121 tgtcaatttc cgcgctatgc acttgtgcgc atactcatgc atgccgtaaa aacagagcct
      181 gcgcgtttct ggcgggtttt cgggtggttt gttgccttgt ttaccggttt cccgtcagaa
      241 acgccctgag ggcatctcag gcggtgctga taacggatgt tatggtaaat caagtgaatg
      301 gcaggtacaa aaaaagcccc agagggcttt tagtttagac ttctgaagat attttatttt
      361 tcttggaagt ggtggttgtt tttgctcgga gggaacttat cacccttgct cacatggcga
      421 tcatattcag aaccagcgta tgagttgtcg gtatcatgat attgaccgta agtacctgtt
      481 tttggacatt cttgcccaga ccaatgtttt gccatttact tttcctttgc tcgttttagg
      541 agctaagctc ctagcggtac gaaattgtgc cgcacttcat aaatgtaatt gcttgaaatt
      601 atgcttcaat gtattgactt gttgtttttg tggttattcg attgagttca cattttgagc
      661 tgatttggtt gtgggttttt agggaaatat ctttatgtac ttatttgcgt acatgttcta
      721 tgctaagccc caacaaatgg gggttaacaa catgcatgca atcaatttta ctgatgcccg
      781 taaacatttt gccgagacga tgaagcgcgt cactgatgat gctgaaccgg tacgtgtcat
      841 gcgccgtgat gcgcctgata ttgttatgat cgatgcggga gagtatgagg cgctaatcga
      901 aacggtctat ctgttcagca atccggcgaa cgcagagcat atcaatgagt caatgaaaca
      961 ggcggagcgc ggcgaatttg tcgaagtgga atattaagtt cactaggaat ggcgctgatg
     1021 accttaacta ctggcgtgat actgacaaaa agaagtttga gcgtatccgg caactgttgc
     1081 tcagcatcga ggctgatccg ctgacaggta taggaaaacc cgaacggctg cggcaccaca
     1141 aagatccggc actctactcc cgcaggattg atcagagcca ccgtctggtc tattcaatcc
     1201 agaacggaga aatcatcatc tacgcagccc gttatcacta tggtgacaaa tagcaaaagt
     1261 cgatatagcc cgcttgatgc gggcttttca tgcaaggcgt acaacggatg ttagggtaag
     1321 tcgctgtcag atcgacacaa aggcaaagtc ggtgtaacgt tagcctcaga aaaaacaaag
     1381 ccccggaaat catgtcatat cttggcggat cgcatgataa ccagggctag gtcgaaacct
     1441 tgaaaggata ttagcacatg aaccgtgcca gacaacagcc cgccataagc gcagggcaca
     1501 ttgcataacg ccgcactcga acttttcaac gaacgcttac cacacaagcc gtacttttcg
     1561 gacgatctgc actttggtgt ccgtatcgcc ggtaaggaac gcgctattct ggcgaaatat
     1621 atccagttca atcagcctca cgccatgttc tggctgggat ttgacgttga ccgcgccggt
     1681 tcctccatcg actggagcga ccgcaacgcg cctgcaccga cgctgaccat aactaacccc
     1741 gaaaacggac acgcgcacct gctgtacgcg ctggaaaact cgatacgcac cgcgccggac
     1801 ggcaaaataa aaccgttacg ctacgccgcc tccgtggaga atgcgctacg taaaaaatta
     1861 gaggctgacg ctggatattc ggggctcatc tgcaaaaatc cgaaccacaa ccactggaaa
     1921 atcgccgtgt ggcagccgaa gctctacact ctggactggc tggccgactt tctcgatctg
     1981 aacacaggga atgacaagga aatcgttgcc gactacggct taggccgtaa ctgcacgttg
     2041 ttcgacaaaa cccgtaagtg ggcatatcgc gccatccgtc agggctggcc tgagtatgag
     2101 cagtggctgc aagcctgcta cgaacgcgct tgcgcataca acctgcaatt tgcacggcct
     2161 ctggatgaaa atgaggtaag agggatagct aagagcattg ctaaatggac atcaaataaa
     2221 ttttcacctg aagaattcag taaatttgtt gatattactc attcctctga aattcaatca
     2281 aagagaggga aaaagtcagg acagtcaagg aggaaaggaa gcctagagga aataaaacct
     2341 tgggtagcta tgggaataag caggcggaag tatttttata tcaagaaaaa tggtgaaata
     2401 agatgaaaac cttcgtaact ctttggacta tagttttttt agcatttatt gccgtgatgt
     2461 gtactgttga tatatctaat tacattccgt ctgtttacac agtttttagt aagaatagac
     2521 ctcttgtaac tggcgccctt tctattcttt tgatatctgt ttttatttgg ctgatggtat
     2581 ccctctactt acttaaaaag tattcggtta aggttgagaa acttagcctt ggtggagtga
     2641 atatactttt caatgagagt gctacacttt acaaaaagag tgtaaaaaat cacctagatt
     2701 caaaaagagc aattttcaaa ttaagaaaaa acgttgatgc ctttgatgag gttatttcat
     2761 catattatca gacttaccaa tttattcgtg atgaaatgaa aatactaaac cctaaaaaag
     2821 atcgtgagat ttatgatttc agcaatgaga tgctcatggt gttaaataga tttctaacta
     2881 actaccagaa caactacaag cgttggtata agtacatatc agacaaagat gaggttgttg
     2941 atagggttag cggtgatgtc atgagatttc atgcaaccag cataaataaa atacaaatgc
     3001 agtattatga ttatgagcgt attttaaaag gttttaaaga tgttaaccgg ttctttgtta
     3061 atgatgtgaa gcctaagttt ggaatcaata cagataagtg gaactggtaa attaaaggag
     3121 tagccaaggc tactccttgg atagtactat gaattatgca ttctcatttc tctcgacata
     3181 tcatacgttg ctttttctct atcgttataa gtcatgtcta ttaattttct gagtgtttct
     3241 gggttgtttt ctatttgatt ccagttgacc actgatatat aagacccgct cttcatgaag
     3301 ttgtctatta ttcttgctgg catatatggg aacctttcct cataagttga gcgcttatca
     3361 atagaaaacc attcagttac ggtaggatga acttctgatt tttcattagt tccatgtgtt
     3421 gaacgaatgt acgtagtatt tgttgacggg agattgacga ctaaaattcc tgattttttg
     3481 ttaatggtac tatctctcat gctagagtaa agttcccaat ctacgtgttt tctgtttttt
     3541 gtttcagttc caacaagaag gataagaact gatgtatctc taagatattc atctcttatt
     3601 atctctctta ttttttgtgg ggcttcatct tcatctatat cacctaatga tactgagcga
     3661 ttcacgaata tttcatgttc ctcattcatt ttttcaagtg cgtttttgta gtactgatca
     3721 ttcgcatggt ggtagctaat gaatacctta tgctttccca tttttattgt cctttttttg
     3781 tttgtgtgca ctctattttg cacttgaacc tatatcagat aacagctctc ttttagagag
     3841 ctcttttttt ctcagccagc acacataggg tcccttcagc aaggtctcgc catgactggt
     3901 ttagggcggg ctcgttgctt ccctaaacgc cttgagaaga gagctatcac ctcactttat
     3961 tcttacgtac cgctcgccgc agtcttatga ccgaacgtag tgagcgaatg cgcgaggaag
     4021 cggaaagtca tttgttgcct gcattttgca cggtcactcc tgtgtttcat cgcccttctg
     4081 aaaaacacat cacttctgga ccagtatgtc gtaagctggc ccctgtttcc ttgagactga
     4141 atgtattaaa ggatgataac tgatgaccag agtgtctatt agtgaggctg cacggctgac
     4201 gggtaagagc cggacaacgc tgcacaggtt aatcaaaaca ggtgaactgt ccacttgtag
     4261 cggtgaacga aatacaaaaa tggtcgatat atcagagctt ttacgtgttt tcggaccgtt
     4321 tgaccaaccg aagaatgaac accttactcg acaagtacct gaacagcgtg atacagcgcc
     4381 tgctgaccag agtgaacatg tcattcgtca actgaaacag cagattgaac accttaacat
     4441 attgatttta tcgaaggatt cgcacattga cagcctgaaa caggccatgc tccttcttga
     4501 aaatcaccag gcaaaattaa ctgaaccccc ggcgccgtgg tggaaattct ggaaaaaatc
     4561 ctgaccccgt catgcttcag ttttcctctc cggccattcc ggggagttcc tgaaccttcc
     4621 cgaaaatcgt cagatctctg aacgctcctc accacgaaaa atacccacac cccacgcaaa
     4681 attttaattt tgcagatttg tatttaatat cagcagctta ctttactgaa atttcgtttt
     4741 ttaaatttta tttatataga tccgactcta tgacgagtcg tcatagagtc cccggcgagt
     4801 cgtcatagag tcggtaacga ctcaggccta taaaaacaat gagttaataa cgacccgact
     4861 ctagatccga ctccccgttt ggttgttttt tagacgtggc gactctgggt acgactctag
     4921 tgaagagtcg ccggggaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc
     4981 cggccgtgga cgaaccgatt cctgaacgtg
//
