LOCUS       NZ_CP125148             6914 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 180 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP125148
VERSION     NZ_CP125148.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790398
            Assembly: GCF_037477765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477765.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,513
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 38 of 109
            Pseudo Genes (incomplete)         :: 60 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 10 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125148.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="180"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            join(5416..6914,1..607)
                     /locus_tag="P4G17_RS27420"
                     /old_locus_tag="P4G17_27400"
     CDS             join(5416..6914,1..607)
                     /locus_tag="P4G17_RS27420"
                     /old_locus_tag="P4G17_27400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_042096180.1"
                     /translation="MKKLSLNWSGNVSQSIEKARTLAAEYSRRDVPDNAPADLRDSASQ
                     GLETSKGFALSGRLLQSELVYGSDVPLAQFGTVLIPLVLAFTTLLLYWLPAWLAFIPSV
                     LWALFAIMTGGFTLGWFAGVLALLLLWGSDISLFSMGGTAQMLQFGTGMTLTLISLAAL
                     PLCHLLRRAERFTIINDDAKAGAIILSASRQAKAREKQTERAARDTSPLIWLGEAQGVT
                     WEKGDIMAPDPASRVALSAADLSTGLLVLGGTGTGKTSGVFRPVIRQWCEHNCGGLLLM
                     DGKGQLPAELAESNRGITLLTPDNPETPVPLIDGLNAEEVAAVLVSVSGKGNDKGSDWT
                     FAAEKQFRFSALLVELAVSVQAAPWTLATIARCVSDTAFRDTLIEAVSARTETLSPELT
                     TAALDFWLKEYPELAKAEGYTSSVQGIVTAWLSPLVGHRKMHGWINAAGGVNPSDVLRG
                     ARYGIGIDSSVYGELCTAAVNGFIKKRLYNEATRRGDNWKADSTNTAAMIVMDEADVLL
                     DETDAGILPRGRSLGLNFVVGLQAVEQAEAAFGGTQNAPKALAMLAQLRSVVALSSTHH
                     TAQYVSDRVGFGLPLEPVSLSTVPDMRYTVLQRAAASPFNDEENEFRSSITLDIPHVIS
                     HTVGKMMDHKNAAGERLQLAKEPKPWISPQECNMLTATEGVAVVVLNRAGAPRRDAIEL
                     VPEYRAG"
                     /name="TraM recognition domain-containing protein"
     gene            complement(635..2074)
                     /locus_tag="P4G17_RS27425"
                     /old_locus_tag="P4G17_27405"
     CDS             complement(635..2074)
                     /locus_tag="P4G17_RS27425"
                     /old_locus_tag="P4G17_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_153937069.1"
                     /translation="MAIARLSMKAGMKGRGADHSAYISREGKYANRLERGERLEATDHG
                     NMPEWAKDNPQQFWLAADAYERKNGTSYREMEIALPRELDSEQREALVRDWTEQELGDK
                     HAYQWAIHVPTAADGGEQPHVHLMFSERQRDGIERDPEQYFKRYNSRNPEAGGARKGYG
                     EHGGQPRTLEQRREELVALRGRWEAKANEHLERAGQRERIDMRSYTAQGKDAIPEKKQL
                     PSEWRDERKRAEILDFRAAKAEQEQAQKDAGKVLYEHNSAVEHYRGVVAAVPEHRLKNS
                     SPDSIALDLREKLVGQRVDSDPAVLAAREQEEKTGKALENARQERDKTRQEAEQQRQEQ
                     QRWEEENRMRRWLHDKGIKKDERYTGLQQQREKSEQQAQAAVQKEQQAEMQHRQAVTAR
                     EETETDVRQQANEKWFEHHHTAVKDAAAERQRELERQEREQQQAKQAEYQQLIAQQQRQ
                     REQEKEQEHTPERQHGRGMSM"
                     /name="MobA/MobL family protein"
     gene            2247..2705
                     /gene="traD"
                     /locus_tag="P4G17_RS27430"
                     /old_locus_tag="P4G17_27410"
     CDS             2247..2705
                     /gene="traD"
                     /locus_tag="P4G17_RS27430"
                     /old_locus_tag="P4G17_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_143550668.1"
                     /translation="MHENEWLEKRLAYIRGLNAPNEQQRLLLMLAQLPQPTPNDRRKLG
                     VLIRAEKAAERALKARADAAKVLNAERRAVRKARDHELYKAAGLMGVAGVVDRETGRLN
                     VDAGLLVGLLMELAAGTLPENLHQEALKSRGDAAIAAREKKNKTNQAG"
                     /name="traD"
     gene            3407..4108
                     /locus_tag="P4G17_RS27435"
                     /old_locus_tag="P4G17_27415"
     CDS             3407..4108
                     /locus_tag="P4G17_RS27435"
                     /old_locus_tag="P4G17_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_153937068.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDAPSPDE
                     QKTMLITAAEFHSAFPDMGRQNAEAKLKEAIDRLWDRSVIIKNEVKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLAVTVETVKKGRTVVALRFR
                     FKEDKQIKMAV"
                     /name="replication initiation protein"
     gene            4105..4230
                     /locus_tag="P4G17_RS27440"
                     /old_locus_tag="P4G17_27420"
     CDS             4105..4230
                     /locus_tag="P4G17_RS27440"
                     /old_locus_tag="P4G17_27420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_255556175.1"
                     /translation="MNNETKKLTLDELLAQCNPDAGMSDEDRQWVNQPPAGDEIA"
                     /name="hypothetical protein"
     gene            4245..4508
                     /locus_tag="P4G17_RS27445"
                     /old_locus_tag="P4G17_27425"
     CDS             4245..4508
                     /locus_tag="P4G17_RS27445"
                     /old_locus_tag="P4G17_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042096185.1"
                     /translation="MNSTTHYENANFLRELAESLPRILPDGGADKAALLQRLANEELAQ
                     AEYDERVRAKVAAARADKRPGMSTAQLRQQLQGRYQELSNDL"
                     /name="hypothetical protein"
     gene            4498..4797
                     /locus_tag="P4G17_RS27450"
                     /old_locus_tag="P4G17_27430"
     CDS             4498..4797
                     /locus_tag="P4G17_RS27450"
                     /old_locus_tag="P4G17_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_042096184.1"
                     /translation="MTYEIDWRPGAVEDMAALFEYLAENASLWDARNVTDRLIAATERL
                     EDYPRLYETDERYGEGVRRISLLGQHVLYEVNDELRRVNILAVVGQRQNPLSVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4805..4966)
                     /locus_tag="P4G17_RS27455"
                     /old_locus_tag="P4G17_27435"
     CDS             complement(4805..4966)
                     /locus_tag="P4G17_RS27455"
                     /old_locus_tag="P4G17_27435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153937067.1"
                     /translation="MTEKKQSANARRQAAFKARQREAGRTQRVLWLTDEEVNAVKALLD
                     KMRGDERP"
                     /name="hypothetical protein"
     gene            5015..5404
                     /locus_tag="P4G17_RS27460"
                     /old_locus_tag="P4G17_27440"
     CDS             5015..5404
                     /locus_tag="P4G17_RS27460"
                     /old_locus_tag="P4G17_27440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153937066.1"
                     /translation="MKKLSVYSRLSRKAGAKIYPNSNPDVHLHKADRIIPVRQEYAGAA
                     AATAGRIAVEVERQTLRSMTYKRLYPWVLALSVLCLLPPFASLGVDMFHAKQAQAEMQR
                     QQKQQLEEMNRRQQERINRLRSGIY"
                     /name="hypothetical protein"
     Typing          3408..4108
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.773"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(639..2074)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.539"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagcacaaat acagcggcca tgattgtgat ggatgaagct gatgtgctgc tcgatgaaac
       61 cgatgccggc attctgccgc gtgggcgctc actggggctg aatttcgtgg ttggcttgca
      121 ggcagtggaa caggcggaag ccgctttcgg cgggacgcag aacgcgccaa aagccctggc
      181 gatgctggca cagctgcgct ccgttgtcgc gctgtcatca acccatcaca ccgcgcagta
      241 tgtctccgat cgtgtggggt tcgggctgcc actggagccg gtatcgctct ccaccgtgcc
      301 ggacatgcgc tataccgtac tgcaacgggc ggcggcctca ccgtttaacg acgaggaaaa
      361 cgagttccgc agctcgataa cgctcgatat tccgcatgtg attagtcata cggttggcaa
      421 aatgatggat cacaaaaatg ctgccggcga acgtctccag ctggcaaaag agcctaagcc
      481 gtggatctca ccgcaggaat gcaacatgct gacggcgacg gagggcgtgg ccgttgtggt
      541 tctcaaccgc gccggcgctc cccgccgcga tgcgatagag ctggttcccg aataccgcgc
      601 cggctgaaaa aaaaggcacc gcaaggtgcc ttttttacat gctcattccg cgcccgtgct
      661 gccgttccgg tgtgtgctcc tgctcttttt cctgctcacg ttgccgctgt tgctgggcga
      721 tcaactgctg atattcagcc tgttttgcct gctgctgctc ccgctcctgc cgctcaagtt
      781 cccgctggcg ttcggcggca gcatctttca cggccgtatg atgatgctcg aaccattttt
      841 cattggcctg ctgacggaca tcagtttcgg tttcttcccg cgcagtgacg gcctgccggt
      901 gctgcatttc tgcctgttgc tccttctgca cggcggcctg tgcctgctgc tctgattttt
      961 cccgttgctg ttgcagtccg gtataacgct cgtccttttt gatgcccttg tcatggagcc
     1021 agcggcgcat ccggttttct tcttcccatc gttgctgctc ctgccgctgc tgctcggctt
     1081 cctgccgggt tttgtcccgt tcctgccgcg cattctccag cgccttaccg gttttttctt
     1141 cctgctcccg tgcggccagt acggccggat cgctgtcaac gcgctgcccg acgagttttt
     1201 ctcgcaaatc cagagcgatg ctgtccggtg aggagttttt cagccggtgc tccggcaccg
     1261 cggccacaac gccgcgatag tgctctaccg cgctgttgtg ctcataaagc accttgccgg
     1321 cgtctttctg tgcctgttcc tgctcggctt ttgcagcgcg gaaatccagt atttctgcac
     1381 gcttccgctc gtcgcgccat tcactcggta gctgcttttt ttcaggaatg gcgtccttgc
     1441 cctgcgctgt atagctgcgc atgtcgatac gttcgcgctg gccggctcgc tcaagatgtt
     1501 cgttagcttt ggcctcccaa cggccgcgca gggcgacaag ttcctcgcgc cgctgctcca
     1561 gcgtgcgcgg ctggccgcca tgctcaccgt accccttccg cgccccgcct gcttccgggt
     1621 tccggctgtt atagcgtttg aaatactgct ctggatcgcg ctcaatcccg tcccgctgcc
     1681 gttcggagaa catcaggtgt acgtgtggct gctcacctcc gtcagcggcg gtgggaacgt
     1741 ggatagccca ctgataagcg tgtttgtcgc ccagctcctg ctccgtccag tccctgacaa
     1801 gcgcctcacg ctgttcgctg tccagctcac gaggcagggc gatttccatc tcacggtatg
     1861 aggtgccgtt cttgcgctca tacgcatcgg cggccagcca gaactgttgc ggattgtcct
     1921 tcgcccactc cggcatattg ccgtggtcgg tggcctccag acgctctccc cgttccagac
     1981 gatttgcata cttgccctca cgggaaatgt aggccgagtg gtctgcgcca cggcctttca
     2041 ttcccgcttt catgctgagt cgtgcaatcg ccatttcttt accctgtaaa tttggggtgt
     2101 ggggcttgcc ccaccgcgca gtttctcacc gaaggggaga aactactaag cgcgccttcc
     2161 gaaaatcgaa gatttccgag aaggatacac cctccgccgc aactgtggta gttttatgca
     2221 ggcacgaaac gagcagcggg ggaaaaatgc acgaaaatga atggctggag aaacggctcg
     2281 catatattcg cggactgaac gcaccgaacg aacagcagcg ccttttgctg atgctggcgc
     2341 agttgcccca gccaacaccg aatgaccgcc gcaagctggg agtactcatt cgtgctgaaa
     2401 aagccgcaga gagggcgcta aaggctcgcg ctgatgccgc taaagtgctg aatgccgaac
     2461 gtcgcgccgt ccgcaaagcg cgtgaccacg aactctacaa ggcggctggt ctgatgggcg
     2521 tggccggcgt ggttgaccgt gaaaccgggc ggctcaatgt ggatgccggc ctgcttgtcg
     2581 gattgctcat ggagttggct gccggtacac tgccggaaaa cctgcaccag gaagcactta
     2641 aatctcgcgg tgatgctgct atcgctgccc gtgagaaaaa aaataaaacg aatcaggctg
     2701 gctaatgaac ctcacacttg tttgccaata gccctgagaa gccgcagaaa tcatcctgcg
     2761 gctttttttt gccttcgggt agggaaacgt acagggaaaa gaaaaaaacg cttacagtgc
     2821 gttatagggc gttttagagc aatccgccaa tttagtgctg gttcccggct gtgacgattg
     2881 ccgcccctcc atcctgaaaa gactggtttc ccccttctct gccagtgaag cgacaccaga
     2941 tcctgacggt cgaagacgta cagcctttca gcgatttgcc cgcccgtccg ggcgggtgct
     3001 acctcctgcc atagcgcgtt attcacaggg atttacggcc attcagcgtc ctgcagaaca
     3061 ggtatattca taacctactg tttattttgg cgtttttttt cgtggcttca ttttatccac
     3121 agcgccgccg ttaagttttt aagatctttt tttttagatc ttctttttta aaaccttttt
     3181 cggtctcgta agccaatgat tctaatcatt ttctggcgag ataggtggag gttttgaagg
     3241 tgataggtgg aggttttgaa ggtgataggt ggaggttttg aaggtgatag gtggaggttt
     3301 tgaaggtgta atccacagaa aatgggggag gataaaaaaa aacttccacc aattagataa
     3361 gtggcgtaca gttaaaaaac accaccaata gcaacgagca agcggaatgt ctgaccttat
     3421 agcctataaa tcaaacgccc ttatcgaggc cagttacaag ctcaccttac aggagcagcg
     3481 cttcctgctg gtctgtatca gccgcctgaa atccggggaa gatgcccctt cgccggatga
     3541 acaaaaaacc atgttaatca cggccgctga gtttcattcg gccttccctg acatggggcg
     3601 gcagaacgct gaagccaagc tcaaggaagc gatagatcgc ctctgggaca ggtcagtcat
     3661 catcaaaaac gaggtaaaac gagaagagtt ccgctgggtg cagtaccggg cgcagtattt
     3721 caaaggtgag gcgaaagtag agatcacctt ctccgatgcc atcatgccct atctgacaca
     3781 gctcaagggg cagtttacgc gggtagtggt gaagaacgtc tccagcctct ccagcaccta
     3841 cagcatccgc atttacgagc tgctccagca gttccgtagt accggtgaca gaacgatagc
     3901 tatcgatgat ttccgaacca tgcttgccgt tgaagataaa tatcaacaat tcaaagattt
     3961 gaataagatg ataatcaagc cagccatcgc tgagctgaac gaaaaaagcg atctggccgt
     4021 caccgtcgag acggtgaaaa aggggcgaac cgttgtcgcg ttgcggttcc ggttcaaaga
     4081 ggacaagcag atcaagatgg ccgtatgaat aacgaaacca aaaagctgac gctggatgaa
     4141 cttctggcac aatgcaatcc cgatgccggc atgagcgatg aagatcgcca gtgggtaaat
     4201 cagccccctg ccggcgatga aatcgcgtga atggagatat gaccatgaac agcactaccc
     4261 actacgaaaa cgccaatttt ctgcgcgaac tggccgagag cttgccccgt attctgccag
     4321 atggcggcgc ggataaggcc gctctgctcc agcgactcgc taacgaagaa ctggcacagg
     4381 ctgaatacga cgagcgggtt cgcgcaaagg ttgccgccgc ccgcgctgac aagcggccgg
     4441 gcatgagtac cgcgcagctg cgccagcagt tacagggacg ctatcaggaa ctgagcaatg
     4501 acctatgaaa tcgactggcg gccgggggcg gttgaagata tggccgccct gtttgagtat
     4561 ctggccgaaa atgcctcgct atgggacgcc agaaacgtga cagaccgcct gatagccgcc
     4621 actgaacgac tggaagatta tccccgtttg tacgagaccg acgagcgata cggcgagggc
     4681 gtccggcgta tcagcctgct cggccagcat gtgctgtatg aggtgaacga cgagttacgg
     4741 cgggtaaaca ttctggccgt ggtcggtcag cgtcagaacc ccttatcggt acgctgaaaa
     4801 cgccttacgg acgttcatcc ccccgcattt tatccagtaa cgccttgacg gcgttcacct
     4861 cttcatcggt cagccacagc acccgctgag tgcggccggc ttctctctgt ctggccttaa
     4921 acgccgcctg acgcctggca tttgctgatt gctttttctc tgtcattcgt tactatccga
     4981 tgcgttacta gtaacgctta aggagtggtt tctgatgaaa aaactgtctg tttattcacg
     5041 tcttagccgg aaagccggcg cgaaaatata ccctaacagt aaccccgatg ttcacctgca
     5101 taaagcagat cgcattatcc ccgtgcgcca ggagtatgcc ggcgcggccg ccgcaacggc
     5161 tggccgcatt gctgtggaag ttgagcgcca gacgctgcgc agcatgacct ataagcgcct
     5221 gtatccgtgg gtgctggcgc tgtccgttct ctgcctgctc ccgccgtttg cctcgctggg
     5281 ggtcgatatg ttccatgcaa aacaggcgca ggcggaaatg cagcgccagc agaaacaaca
     5341 gctggaagag atgaatcgcc ggcagcagga aagaattaat cgcctgcgca gcggcattta
     5401 ctgagggatc agaacatgaa aaaactgtca cttaactggt ctggcaatgt gagtcagtcc
     5461 atcgaaaaag cccgcacact ggccgccgaa tactcccgcc gtgatgtacc cgacaacgca
     5521 ccggccgatc tgcgcgactc ggccagtcag ggactggaaa cgagcaaggg gtttgcgttg
     5581 tctggccgtc tgctgcaaag cgaactggtc tacggttcag atgtgccgct ggcgcagttc
     5641 ggcactgtgc tgatcccgct ggtactggcg ttcacaaccc tgcttctgta ctggctcccg
     5701 gcatggctgg cattcattcc gtccgtctta tgggcgctgt ttgccatcat gaccggtggt
     5761 tttacgctgg gctggtttgc cggcgttctg gcgctgctcc tgctctgggg atctgatatt
     5821 tcgctgtttt cgatgggcgg gacagcgcag atgctccagt ttggcaccgg tatgacgctg
     5881 acgctgattt cgctggccgc tctgccgctc tgtcaccttc tgcgccgcgc cgagcgcttt
     5941 accatcatca acgatgacgc taaagccggc gcaatcattc tgtccgcctc acgtcaggca
     6001 aaagcccgcg aaaagcagac agaacgagcc gcacgcgaca cctcgccgct gatctggctg
     6061 ggtgaggcgc agggcgtgac atgggagaag ggggatatta tggcgcccga tccggcgtca
     6121 cgcgttgccc tgtcggccgc tgacctgtcc acgggattgc ttgttctcgg tggcaccggc
     6181 accggcaaaa cgtccggcgt gttcaggccg gttatccgtc agtggtgtga gcacaattgc
     6241 ggtggcctgc ttctgatgga cggaaagggg cagttaccgg ccgaactggc cgaaagcaac
     6301 aggggaataa cgctgctgac cccggataac ccggaaacgc ctgtcccgct gatcgatggc
     6361 ctgaatgccg aagaagtggc cgccgtgctg gtttctgtct ccggcaaggg gaatgataaa
     6421 ggctctgact ggacatttgc ggccgaaaaa cagttccgtt tttctgccct gctcgttgag
     6481 ctggccgtca gcgttcaggc cgcaccgtgg acgctggcca ctattgcccg ctgcgtgtca
     6541 gatacggcat tcagggacac gctgattgag gctgtaagcg cccgtacaga aacactgtca
     6601 cctgaactga ccacggccgc actggatttc tggctcaagg aatacccgga actggcaaaa
     6661 gccgaagggt ataccagttc tgtgcagggg attgtgaccg cgtggctttc tccgctggtc
     6721 ggtcacagga aaatgcacgg ctggattaat gccgccggag gggtaaaccc ttctgatgtc
     6781 ctgcgcgggg cgcggtacgg catagggatt gattcatcgg tatatggcga actctgcacc
     6841 gcagcggtta acgggttcat caaaaaacgg ctgtacaacg aggcaacgcg gcgcggtgat
     6901 aactggaaag cgga
//
