LOCUS       NZ_CP125147             8351 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 180 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP125147
VERSION     NZ_CP125147.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790398
            Assembly: GCF_037477765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477765.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,513
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 38 of 109
            Pseudo Genes (incomplete)         :: 60 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 10 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="180"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            1..840
                     /locus_tag="P4G17_RS27360"
                     /old_locus_tag="P4G17_27340"
     CDS             1..840
                     /locus_tag="P4G17_RS27360"
                     /old_locus_tag="P4G17_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="P4G17_RS27365"
                     /old_locus_tag="P4G17_27345"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="P4G17_RS27365"
                     /old_locus_tag="P4G17_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            <1853..2500
                     /locus_tag="P4G17_RS27370"
                     /old_locus_tag="P4G17_27350"
                     /pseudo=""
     CDS             <1853..2500
                     /locus_tag="P4G17_RS27370"
                     /old_locus_tag="P4G17_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family protein"
                     /name="aminoglycoside phosphotransferase family protein"
     gene            2905..3684
                     /locus_tag="P4G17_RS27375"
                     /old_locus_tag="P4G17_27355"
     CDS             2905..3684
                     /locus_tag="P4G17_RS27375"
                     /old_locus_tag="P4G17_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_094965899.1"
                     /translation="MKLPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTATTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEVLNLLNSIAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            3728..3928
                     /locus_tag="P4G17_RS27380"
                     /old_locus_tag="P4G17_27360"
     CDS             3728..3928
                     /locus_tag="P4G17_RS27380"
                     /old_locus_tag="P4G17_27360"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094965900.1"
                     /translation="MLWTVDAEQDRERIYDYLDERNPIAAIELDDLIREKVSLLAHNNL
                     MGRTGQKKGTRELKTMSEANP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(<4270..4674)
                     /locus_tag="P4G17_RS27385"
                     /old_locus_tag="P4G17_27365"
                     /pseudo=""
     CDS             complement(<4270..4674)
                     /locus_tag="P4G17_RS27385"
                     /old_locus_tag="P4G17_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(5228..5512)
                     /locus_tag="P4G17_RS27390"
                     /old_locus_tag="P4G17_27370"
     CDS             complement(5228..5512)
                     /locus_tag="P4G17_RS27390"
                     /old_locus_tag="P4G17_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5712..>6353
                     /locus_tag="P4G17_RS27395"
                     /old_locus_tag="P4G17_27375"
                     /pseudo=""
     CDS             5712..>6353
                     /locus_tag="P4G17_RS27395"
                     /old_locus_tag="P4G17_27375"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6460..6873
                     /locus_tag="P4G17_RS27400"
                     /old_locus_tag="P4G17_27380"
     CDS             6460..6873
                     /locus_tag="P4G17_RS27400"
                     /old_locus_tag="P4G17_27380"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6972..7841
                     /locus_tag="P4G17_RS27405"
                     /old_locus_tag="P4G17_27385"
     CDS             6972..7841
                     /locus_tag="P4G17_RS27405"
                     /old_locus_tag="P4G17_27385"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7902..8114
                     /locus_tag="P4G17_RS27410"
                     /old_locus_tag="P4G17_27390"
     CDS             7902..8114
                     /locus_tag="P4G17_RS27410"
                     /old_locus_tag="P4G17_27390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            8116..8322
                     /gene="urfF"
                     /locus_tag="P4G17_RS27415"
                     /old_locus_tag="P4G17_27395"
     CDS             8116..8322
                     /gene="urfF"
                     /locus_tag="P4G17_RS27415"
                     /old_locus_tag="P4G17_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             2906..3681
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.07"
                     /coverage="100.0"
     ARG             1872..2500
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.04"
                     /coverage="75.18"
     Typing          5467..5623
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.367"
                     /coverage="100.0"
                     /evalue="2.97e-75"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5713..7838
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgggagggg
     1861 agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg
     1921 aatgctctct cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga
     1981 actaatggcg aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat
     2041 ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca
     2101 aactgactac gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact
     2161 gcgtgggcta catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct
     2221 ggtgatagat cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta
     2281 cgatccggct gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga
     2341 cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta
     2401 tgggtgcctt tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat
     2461 cgcggccgcg atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta
     2521 tcccctggga acacatcaat cttaccggag aatatcgttg gccaaagcct tagcgtagga
     2581 tttcgccctc tcccgcaaac gaccccgaca aggcaattga ttaagtgctt aatgattgaa
     2641 tatttgacac aggtcaacgc atcagcttgt ttaagatatg gagaaacaag atgattgatc
     2701 cctaaaatca tagcgcaact cgggtttgat taaaaaaaag cctctttttt aagaggagct
     2761 acagctgtgt cggctgggac aataattttg caagttcttc tagggaataa gccatatttt
     2821 tgctacagtg aaccaaatta agatcatcta tttactaggc ctcgcatttg cggggttttt
     2881 aatactgaat aaaaggaaag cttgatgaaa ttgcccaata ttattcaaca atttattgga
     2941 aacagcgttt tagagccaaa taaaattggt cagtcgccat cggatgttta ttcttttaat
     3001 cgaaataatg aaactttttt tcttaagcga tctagcactt tatatacagc gaccacatac
     3061 agtgtttctc gtgaagcgaa aatgttgagt tggctctctg agaagttaaa ggtgcctgaa
     3121 ctcatcatga cttttcagga tgagcagttt gaattcatga tcactaaagc gatcaatgca
     3181 aaaccaattt cagcgctttt tttaacagac caagaattgc ttgctatcta taaggaggta
     3241 ctcaatctgt taaattcaat tgctattatt gattgtccat ttatttcaaa cattgatcat
     3301 cggttaaaag agtcaaaatt ttttattgat aaccaactcc ttgatgatat agatcaagat
     3361 gattttgacg ctgaattatg gggagaccat aaaacttacc taagtctatg gaatgagtta
     3421 aatgagactc gtgttgaaga aagattggtt ttttctcatg gcgatatcac ggatagtaat
     3481 atttttatag ataaattcaa tgaaatttat tttttagatc ttggccgtgc tgggttagca
     3541 gatgagtttg tagatatatc ctttgttgaa cgttgcctaa gagaggatgc atcggaggaa
     3601 actgctaaaa tatttttaaa gcatttaaaa aatgatagac ctgacaaaag gaattatttt
     3661 ttaaaacttg atgaattgaa ttgattccaa gcattttcta aaaatactta attgtcattt
     3721 tacgtcgatt ctttggaccg tagatgcaga acaagatcgt gaaagaattt atgattatct
     3781 tgatgaaaga aatccaattg ctgcaattga attggatgat ttaattcggg aaaaagttag
     3841 tttgcttgct cataataatc tcatggggag aactggacag aaaaaaggaa caagagagtt
     3901 aaaaactatg agcgaagcga atccatagtc gcttttgctt tttgaagtca caaacaggat
     3961 tagaaaataa ctgcccccac gaccgagcaa agcgaggtca aaaagtacga gcaaagcgag
     4021 tacatagggc agttatccac aggttttttt aaattttttc tttttttaat attttttaat
     4081 atttttaaat atttttagct cctcgctatg ccttgctcta cgtggctttg acgggggtaa
     4141 ctatgacaga ttgacgcaca actatatcaa gcgacttctc ctatcccctg ggaacacatc
     4201 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     4261 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     4321 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4381 acagctcggg cagaaaccgt gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4441 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4501 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4561 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4621 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4681 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4741 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccgtactaa
     4801 ctgtcacgaa cccctgcaat aactgtcacg cccccctgca ataactgtca cgaacccctg
     4861 caataactgt cacgccccca aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt
     4921 ggaaaaatcc atccatgatt atctaagaat aatccactag gcgcggttat cagcgccctt
     4981 gtggggcgct gctgcccttg cccaatatgc ccggccagag gccggatagc tggtctattc
     5041 gctgcgctag gctacacacc gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc
     5101 cgctaaaccc cacaccaaac cccgcagaaa tacgctggag cgcttttagc cgctttagcg
     5161 gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt
     5221 cgatcattca gcccggctca tccttctggc gtggcggcag accgaacaag gcgcggtcgt
     5281 ggtcgcgttc aaggtacgca tccattgccg ccatgagccg atcctccggc cactcgctgc
     5341 tgttcacctt ggccaaaatc atggccccca ccagcacctt gcgccttgtt tcgttcttgc
     5401 gctcttgctg ctgttccctt gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt
     5461 gttcttcgag cttggccagc cgatccgccg ccttgttgct ccccttaacc atcttgacac
     5521 cccattgtta atgtgctatc tcgtaggcta tcatggaggc acagcggcgg caatcccgac
     5581 cctactttgt agggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc
     5641 cacccttcgg gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac
     5701 agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc
     5761 aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg
     5821 atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact
     5881 actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat
     5941 ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc
     6001 agcacctgac cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg
     6061 agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg
     6121 ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga
     6181 ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg
     6241 ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg
     6301 cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag
     6361 gccggggcat ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc
     6421 agatcatcga cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg
     6481 aagaaatcca gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg
     6541 gcgacactgg ccgacgaagc ccggcaggtc atgagccaga cccagcaggc cagcgaggcg
     6601 caggcggcgg agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc
     6661 aaagagttgc gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg
     6721 tcgcgggggt ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg
     6781 gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac
     6841 ggctcgatct ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag
     6901 gccgacagct caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgccacca
     6961 ccggccagat gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc
     7021 tcaagcggat gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc
     7081 atggtctggt gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg
     7141 gccgggagcc tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg
     7201 tggccgacgc cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt
     7261 acgacgccga cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca
     7321 accgcaagga caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat
     7381 ccaagggcaa gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg
     7441 agcaggccca gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc
     7501 ggcagcttag ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc
     7561 tggtcaagcg cttcggtgat gacctcagca agtgcgactt tatcgccgcg cagaagctgg
     7621 ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc
     7681 tggcagagcg caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca
     7741 tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc
     7801 agcgaggcat ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac
     7861 gcctgttata ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg
     7921 cttcagggtc ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg
     7981 gttatacgtc aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta
     8041 accttgacgg ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt
     8101 ttctcggtga ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc
     8161 cctgacgctg tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag
     8221 cgcaagttct ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc
     8281 agagcggcgc agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa
     8341 ggaggcaatc a
//
